• Compare Products

  • Table of Contents

ELISA Kits -> High Sensitivity ELISA Kits


BOSTER BIO HIGH SENSITIVITY ELISA KITS



Detect low-abundance biomarkers with sub-pg/mL sensitivity. Reliable results from even the most challenging samples.

Featured High Sensitivity Kits:

Browse All High Sensitivity ELISA Kits

Download Flyer

Boster Bio ELISA Kits Flyer 2023

Browse high sensitivity elisa kits

Product
Figures
Species
Applications
Price
sensitivity
assay range
Mouse GM-CSF High Sensitivity ELISA Kit PicoKine®

hsek0365_1

1 images

Mouse
ELISA
$519
Cat # HSEK0365
96 wells/kit, with removable strips.
<0.1 pg/ml
1.56-100 pg/ml
Human G-CSF High Sensitivity ELISA Kit PicoKine®

hsek0360_1

1 images

Human
ELISA
$519
Cat # HSEK0360
96 wells/kit, with removable strips.
<0.3 pg/ml
4.69-300 pg/ml
Rat TNF Alpha/Tumor necrosis factor High Sensitivity ELISA Kit PicoKine®

hsek0526_1

1 images

Rat
ELISA
$519
Cat # HSEK0526
96 wells/kit, with removable strips.
<0.006 pg/ml
1.56-100 pg/ml
Human IL-10/Interleukin-10 High Sensitivity ELISA Kit PicoKine®

hsek0416_3

1 images

Human
ELISA
$519
Cat # HSEK0416
96 wells/kit, with removable strips.
<0.06 pg/ml
0.78-50pg/ml
Mouse IL-6 High Sensitivity ELISA Kit PicoKine®

hsek0411

1 images

Mouse
ELISA
$519
Cat # HSEK0411
96 wells/kit, with removable strips.
<0.17 pg/ml
1.56-100pg/ml
Mouse IFN Gamma High Sensitivity ELISA Kit PicoKine®

hsek0375

1 images

Mouse
ELISA
$519
Cat # HSEK0375
96 wells/kit, with removable strips.
<0.3 pg/ml
3.12-200pg/ml
Mouse IL-2 High Sensitivity ELISA Kit PicoKine®

hsek0398

1 images

Mouse
ELISA
$519
Cat # HSEK0398
96 wells/kit, with removable strips.
<0.005 pg/ml
0.31-20pg/ml
Mouse IL-17 High Sensitivity ELISA Kit PicoKine®

hsek0431

1 images

Mouse
ELISA
$519
Cat # HSEK0431
96 wells/kit, with removable strips.
<0.03 pg/ml
0.78-50pg/ml
Mouse IL-1 Beta High Sensitivity ELISA Kit PicoKine®

hsek0394

1 images

Mouse
ELISA
$519
Cat # HSEK0394
96 wells/kit, with removable strips.
<0.23 pg/ml
1.56-100pg/ml
Mouse TNF Alpha High Sensitivity ELISA Kit PicoKine®

hsek0527

1 images

Mouse
ELISA
$519
Cat # HSEK0527
96 wells/kit, with removable strips.
<0.006 pg/ml
1.56-100pg/ml

Displaying Items 1-10 of Many

Why Choose Boster Bio High Sensitivity ELISA Kits?

For over 30 years, Boster Bio has specialized in developing ultrasensitive immunoassays for life science research. Our High Sensitivity ELISA platform is engineered to overcome the limitations of standard kits when working with low-concentration targets.

Whether you’re studying immune regulation, biomarker discovery, or preclinical drug response, our High Sensitivity kits provide publication-ready data you can trust.

Why High Sensitivity Matters

  • When analytes sit near baseline, conventional ELISA often shows high CV or non-detects.
  • High sensitivity improves signal-to-noise in the low pg/mL range.
  • Better low-end performance supports early timepoints, small fold-changes, and limited sample volume.

High Sensitivity vs. Traditional ELISA

Feature High Sensitivity ELISA Traditional ELISA
Detection limit (LOD) Sub-pg/mL (selected kits down to 0.005 pg/mL) Varies by assay; higher LOD may lead to non-detects at low levels
Low-end precision Improved precision at low concentrations (kit-dependent) Often higher variability near the detection limit
Non-detect rate Fewer non-detects in baseline/early samples More non-detects when analyte concentration is low
Sample flexibility Allows greater dilution to reduce matrix effects while staying detectable* Limited dilution flexibility for low-abundance targets
Best for Baseline cytokines, limited-volume samples, early timepoints Stimulated/high-expression samples, routine screening
Workflow Standard ELISA protocol — no special equipment required Standard ELISA protocol

*Higher sensitivity enables larger sample dilution to minimize matrix interference while maintaining detectable signal.

Analyze Your Data with Confidence—Free ELISA Calculator Included

Struggling with curve fitting or concentration interpolation?

Use our free online ELISA data analysis tool to:

  • ● Automatically fit 4PL/5PL standard curves
  • ● Calculate sample concentrations from OD values
  • ● Troubleshoot common assay issues

No software download required. Works with any Boster or third-party ELISA kit.

Try the Free ELISA Calculator Now

Performance Validation: Human IL-10/Interleukin-10 High Sensitivity ELISA Kit PicoKine®

Price: $519
Size: 96 wells/kit, with removable strips.
Reactivity: Human
Sample Type: Cell culture supernatants, serum, and plasma (heparin, EDTA, citrate).
Application: ELISA

Real validation data (from Boster Bio product page)

Sensitivity: < 0.06 pg/mL
Assay Range: 0.78 – 50 pg/mL

Key Highlights:

  • Detection limit < 0.06 pg/mL enables quantification of baseline IL-10 in healthy human serum
  • Consistent precision even at low concentrations (CV < 8% at 1 pg/mL)
  • Linear response across full assay range
  • No detectable cross-reactivity with other cytokines
  • Validated for direct use in serum/plasma without extensive pre-treatment

Intra-assay Precision (16 replicates per concentration):

  • Low concentration (1 pg/mL): Mean 1.00 pg/mL, CV 7.1%
  • Medium concentration (4 pg/mL): Mean 4.00 pg/mL, CV 5.8%
  • High concentration (16 pg/mL): Mean 16.00 pg/mL, CV 3.2%

Inter-assay Precision (24 replicates across batches):

  • Low concentration (1 pg/mL): Mean 1.00 pg/mL, CV 4.9%
  • Medium concentration (4 pg/mL): Mean 4.00 pg/mL, CV 4.3%
  • High concentration (14 pg/mL): Mean 14.00 pg/mL, CV 3.2%

Standard Curve OD450nm Values:

  • 0 pg/mL: 0.029
  • 0.78 pg/mL: 0.120
  • 1.56 pg/mL: 0.210
  • 3.13 pg/mL: 0.383
  • 6.25 pg/mL: 0.725
  • 12.5 pg/mL: 1.235
  • 25 pg/mL: 2.044
  • 50 pg/mL: 3.250

Data source: Boster Bio HSEK0416 product validation page (accessed January 2026)


Technical Features

Parameter Performance
Sensitivity Sub-pg/mL range (as low as 0.005 pg/mL, depending on target)
Precision Intra-assay CV < 10% • Inter-assay CV within validated range
Validated Sample Types Serum, Plasma (EDTA/heparin/citrate), Cell Culture Supernatants
Format 96-well strip plate with removable strips
Technology Platform PicoKine® High Sensitivity system

High Sensitivity ELISA kit components:

• Pre-coated 96-well Strip Microplate.

• Standard

• Detection Antibody

• Sample Diluent

• Wash Buffer

• Detection Antibody Diluent

• Color Developing Reagent (TMB)

• Stop Solution

• Plate Sealers

Materials that are required but not provided:

• Microplate Reader capable of reading absorbance at 450 nm.

• Automated plate washer (optional)

• Pipettes and pipette tips capable of precisely dispensing 0.5 µL to 1 mL volumes of aqueous solutions.

• Multichannel pipettes are recommended for large amount of samples.

• Deionized or distilled water.

• 500mL graduated cylinders.

• Test tubes for dilution.

FAQS

Common questions about performance, workflow, and data analysis for High Sensitivity ELISA kits

High Sensitivity ELISA kits are designed to quantify low-abundance targets with improved low-end detectability and resolution compared with standard ELISA kits.

Choose High Sensitivity ELISA when targets are expected near baseline/low pg/mL levels, sample volume is limited, or you need to resolve small fold-changes. For strongly induced or high-expression samples, standard ELISA may be sufficient and more cost-efficient.

No. High Sensitivity ELISA kits use a standard ELISA workflow and standard plate readers—no special equipment is required.

LOD reflects the lowest level distinguishable from background, while the assay range (quantifiable range) defines the concentrations that can be quantified reliably on the standard curve (typically between the lowest and highest standards).

Sample compatibility varies by kit. Please follow the sample types listed on each product page (e.g., supernatants, serum, plasma with specified anticoagulants, and selected kits validated for cell lysates). For best comparability, avoid mixing matrices within the same analysis unless validated.

Yes. Higher sensitivity can support additional dilution while maintaining detectable signal. For best accuracy, verify dilution linearity and spike recovery in your sample matrix before large-scale experiments.

If results fall below the lowest standard/quantifiable range, report as "< LLOQ" (below the lower limit of quantification). Consider concentrating the sample (if feasible), reducing dilution, or selecting a kit with a lower working range.

Run standards and samples in duplicates (triplicates recommended for low-abundance targets). Use 4PL/5PL curve fitting as recommended by the kit/software. Avoid comparing data across plates without consistent controls.