Human IL-6 High Sensitivity ELISA Kit PicoKine®

Product Name

Human IL-6 High Sensitivity ELISA Kit PicoKine®

SKU/Catalog Number

HSEK0410

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human IL-6/Interleukin-6 ELISA Kit PicoKine® (96 Tests). Quantitate Human IL6 in cell culture supernatants, serum and plasma ( heparin, EDTA, citrate). Sensitivity: 0.108pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human IL-6 High Sensitivity ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # HSEK0410)

Clonality of Antibodies

See Datasheet for details

Standard Protein

Expression system for standard: E.coli; Immunogen sequence: P29-M212

Capture/Detection Antibodies

monoclonal antibody from mouse|polyclonal antibody from goat

Sensitivity

<0.108 pg/ml

Assay Range

0.78-50pg/ml

Standard Dilution Instructions

See datasheet of HSEK0410 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

HSEK0410 is reactive to IL6 in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma ( heparin, EDTA, citrate).

Application Guarantee

HSEK0410 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of IL6

The human interferon-beta 2 gene (IFNB2) product is identical to that for the B-cell stimulation factor-2 (BSF-2), the hybridoma growth factor (HGF) ("interleukin-6"), and the hepatocyte stimulating factor (HSF). Proteins derived from this gene mediate the plasma protein response to tissue injury (acute-phase response) and regulate the growth and differentiation of both B and T cells. Interleukin-6 (IL6) has come to be regarded as a potential osteoporotic factor because it has stimulatory effects on cells of the osteoclast lineage, and, thus, may play a role in the pathogenesis of bone loss associated with estrogen deficiency. IL-6 has many roles essential to the regulation of the immune response, hematopoiesis, and bone resorption. It is involved not only in the hepatic acute phase response but also in adipose tissue metabolism, lipoprotein lipase activity, and hepatic triglyceride secretion. Overproduction of IL-6, a proinflammatory cytokine, is associated with a spectrum of age-related conditions including cardiovascular disease, osteoporosis, arthritis, type 2 diabetes, certain cancers, periodontal disease, frailty, and functional decline. BSF-2 is a novel interleukin consisting of 184 amino acids. The standard product used in this kit is recombinant human IL-6, consisting of 184 amino acids with the molecular mass of 20.3kDa.

Kit Components

Catalog Number	Description	Quantity
HSEK0410-CAP	Anti-Human IL6 Pre-coated 96-well strip microplate	1
HSEK0410-ST	Human IL6 Standard	2 vials, 10 ng/tube
HSEK0410-DA	Human IL6 Biotinylated antibody (100x)	200ul
AR1103	Avidin-Biotin-Peroxidase Complex (100x)	200ul
AR1106-1	Sample Diluent	30mlx2
AR1106-2	Antibody Diluent	25ml
AR1106-3	Avidin-Biotin-Peroxidase Diluent	25ml
AR1104	Color Developing Reagent (TMB)	20ml
AR1105	Stop Solution	10ml
AR1106-5	Wash Buffer (25x)	20ml
PLA-SEA	Adhesive plate sealers	4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

• Microplate Reader capable of reading absorbance at 450nm.
• Incubator.
• Automated plate washer (optional).
• Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
• Multichannel pipettes are recommended for large amount of samples.
• Deionized or distilled water.
• 500ml graduated cylinders.
• Test tubes for dilution.

Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)	0	0.78	1.56	3.12	6.25	12.5	25	50
O.D.	0.056	0.125	0.151	0.233	0.321	0.618	1.12	1.707

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human IL-6 High Sensitivity ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

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