Introduction
Boster's ELISA Kit is for the detection of cytoplasmic Plant Hsp70 from plant extracts. But this kit is also compatible with human tissue extracts and lysates and serum and plasma. Each kit contains sufficient components to quantitate the Hsp70 concentration in up to 40 samples, tested in duplicate.
Overview
| Product Name |
HSP70 ELISA Kit (PLANT)
|
| Catalog# |
EK7115 |
| Storage & Handling |
Store at 4°C. |
| Description |
Colorimetric detection used to quantitate HSP70 in plants. 96wells/kit, with removable strips. |
| Cite This Product |
HSP70 ELISA Kit (PLANT) (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK7115)
|
Product Specs
| Reactivity/Species |
Alfalfa, Flax, Human, Maize, Plant |
| Applications |
ELISA
*Our Boster Guarantee covers the use of this product in the above
tested applications.
**For positive and negative control design, consult "Tissue specificity" under
"Protein Target Info" tab. |
| Sample Type |
Cell lysates, Plant extract, Plasma, Serum, Tissue |
| Sensitivity |
0.18 ng/ml |
| Assay Range |
1.563 - 100 ng/ml |
| Cross Reactivity |
There is no detectable cross-reactivity. |
| Pack Size |
96wells/kit |
Gene/Protein Basic Information For Hspa1b (Source: Uniprot.org, NCBI)
| Uniprot Id | P0DMV8/P0DMV9 |
|---|
| Gene Name | Hspa1b |
|---|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Hspa1b, check out the Hspa1b Infographic
We have 30,000+ of these available, one for each gene! Check them out.
Want a nice infographic on your next protein? Boster's got them all. All proteins in the human genome, and some, can be found in the Boster Bio gene infographics. Download it and share it with your colleagues and friends. Big size posters available upon request.
In this infographic you will see the following information for Hspa1b: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. Some times it even contains some opt-ed articles the Boster team on the scientific news and clinical impacts of this protein, though not all have that. Too many proteins, you know.
Take me to the Hspa1b infographic
Kit Components And Assay QC Data Details Of HSP70 ELISA Kit (PLANT)
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
| Description | Quantity |
| Anti-Hsp70 Immunoassay Plate | 1 Plate |
| 5X Hsp70 Extraction Reagent | 1 vial/10 ml |
| Recombinant Hsp70 (Alfalfa) Standard | 2 vials |
| Standard and Sample Diluent | 1 vial/ 50 ml |
| 10X Wash Buffer Concentrate | 1 vial/100 ml |
| Anti-Hsp70 Biotinylated Antibody Concentrate | 1 vial/150 µl |
| Anti-Hsp70 Biotinylated Antibody Diluent | 1 vial/ 13 ml |
| Streptavidin: HRP Concentrate | 1 vial/150 µl |
| Streptavidin: HRP Diluent | 1 vial/ 13 ml |
| TMB Substrate | 1 vial/ 13 ml |
| Stop Solution | 1 vial/ 13 ml |
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EK7115 HSP70 ELISA Kit (PLANT). It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 30 minutes
We measured random samples of HSP70 ELISA Kit (PLANT) within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
We measured the performance consistency of HSP70 ELISA Kit (PLANT) to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
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