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Product Info Summary
| SKU: | PA1813 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, ICC, WB |
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Product info
Product Name
Anti-Hsp70/HSPA1A/HSPA1B Antibody Picoband®
SKU/Catalog Number
PA1813
BA3769 is an alternative SKU for this antibody, used in previous lots.
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Hsp70/HSPA1A/HSPA1B Antibody catalog # PA1813. Tested in IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Hsp70/HSPA1A/HSPA1B Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1813)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.01mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Hsp70, different from the related mouse sequence by four amino acids and from the related rat sequence by three amino acids.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1813 is reactive to HSPA1A in Human, Mouse, Rat
Observed Molecular Weight
70 kDa
Calculated molecular weight
70.1 kDa
Background of HSPA1A
HSPA1 (heat shock 70kDa protein 1A) also known as HSP70-1, HSPA1A, HSP70-1A, HSP72 or HSP70I, is a protein that in humans is encoded by the HSPA1A gene. This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. The HSPA1A gene encodes a predicted 641-amino acid protein. The HSPA1 gene is mapped on 6p21.33. Shimizu et al. (1999) found that peripheral blood mononuclear cells of 18 major depression patients expressed a short HSPA1A transcript that utilized exon 1 rather than exon 2, which is found in the more common HSPA1A transcript. No protein was associated with expression of this short HSPA1A mRNA, possibly due to lack of a TATA box or loss of internal ribosome binding sites. Treatment with BGP-15, a pharmacologic inducer of Hsp72 that can protect against obesity-induced insulin resistance, improved muscular architecture, strength, and contractile function in severely affected diaphragm muscles in mdx dystrophic mice.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1813 is guaranteed for IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Rat
Immunocytochemistry , 0.5-1μg/ml, Human, -
Positive Control
WB: human Hela whole cell, human HepG2 whole cell, human A431 whole cell, human MCF-7 whole cell, human A549 whole cell, human PC-3 whole cell, human 293T whole cell, human K562 whole cell, rat liver tissue, rat kidney tissue, rat brain tissue, rat RH35 whole cell, mouse liver tissue, mouse kidney tissue, mouse brain tissue, mouse NIH/3T3 whole cell
IHC: Human Intestinal Cancer Tissue
IHC-F: Rat Intestine Tissue
ICC: A549 Cell
Validation Images & Assay Conditions
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Western blot analysis of HSP70 using anti-HSP70 antibody (PA1813).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human A431 whole cell lysates,
Lane 4: human MCF-7 whole cell lysates,
Lane 5: human A549 whole cell lysates,
Lane 6: human PC-3 whole cell lysates,
Lane 7: human 293T whole cell lysates,
Lane 8: human K562 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSP70 antigen affinity purified polyclonal antibody (Catalog # PA1813) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSP70 at approximately 70 kDa. The expected band size for HSP70 is at 70 kDa.
Click image to see more details
Western blot analysis of HSP70 using anti-HSP70 antibody (PA1813).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat liver tissue lysates,
Lane 2: rat kidney tissue lysates,
Lane 3: rat brain tissue lysates,
Lane 4: rat RH35 whole cell lysates,
Lane 5: mouse liver tissue lysates,
Lane 6: mouse kidney tissue lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSP70 antigen affinity purified polyclonal antibody (Catalog # PA1813) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HSP70 at approximately 70 kDa. The expected band size for HSP70 is at 70 kDa.
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Anti-Hsp70 antibody, PA1813, IHC(F)
IHC(F):Rat Intestine Tissue
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Anti-Hsp70 antibody, PA1813, IHC(P)
IHC(P): Human Intestinal Cancer Tissue
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Anti-Hsp70 antibody, PA1813, ICC
ICC: A549 Cell
Specific Publications For Anti-Hsp70/HSPA1A/HSPA1B Antibody Picoband® (PA1813)
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Customer Q&As
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18 Customer Q&As for Anti-Hsp70/HSPA1A/HSPA1B Antibody Picoband®
Question
Does PA1813 have any reactivity with mice?
Verified customer
Asked: 2022-04-26
Answer
Yes, the Anti-Hsp70/HSPA1A/HSPA1B Antibody (PA1813) has cross reactivity with mouse samples.
Boster Scientific Support
Answered: 2022-04-27
Question
Do you have a BSA free version of anti-Hsp70/HSPA1A/HSPA1B antibody PA1813 available?
Verified Customer
Verified customer
Asked: 2020-03-23
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Hsp70/HSPA1A/HSPA1B antibody PA1813 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-03-23
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for colon carcinoma using anti-Hsp70/HSPA1A/HSPA1B antibody PA1813. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-12-10
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-10
Question
My question regards using your anti-Hsp70/HSPA1A/HSPA1B antibody for atp metabolic process studies. Has this antibody been tested with western blotting on panc whole cell lysate? We would like to see some validation images before ordering.
T. Edwards
Verified customer
Asked: 2019-11-07
Answer
Thanks for your inquiry. This PA1813 anti-Hsp70/HSPA1A/HSPA1B antibody is validated on 293t whole cell lysate, hela whole cell lysate, a549 whole cell lysate, a431 whole cell lysate, panc whole cell lysate, intestinal cancer tissue. It is guaranteed to work for IHC, ICC, WB in human, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-11-07
Question
My team were content with the WB result of your anti-Hsp70/HSPA1A/HSPA1B antibody. However we have observed positive staining in pancreas cytoplasm. using this antibody. Is that expected? Could you tell me where is HSPA1A supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-10-31
Answer
Based on literature, pancreas does express HSPA1A. Generally HSPA1A expresses in cytoplasm. Regarding which tissues have HSPA1A expression, here are a few articles citing expression in various tissues:
Brain, Cajal-Retzius cell, and Fetal brain cortex, Pubmed ID: 23349634
Brain, Muscle, Pancreas, PNS, and Skin, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 17081983, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Colon carcinoma, Pubmed ID: 24129315
Liver, Pubmed ID: 24275569
Uterus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2019-10-31
Question
Is a blocking peptide available for product anti-Hsp70/HSPA1A/HSPA1B antibody (PA1813)?
Verified Customer
Verified customer
Asked: 2019-09-09
Answer
We do provide the blocking peptide for product anti-Hsp70/HSPA1A/HSPA1B antibody (PA1813). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-09-09
Question
We are currently using anti-Hsp70/HSPA1A/HSPA1B antibody PA1813 for rat tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human, rat. Is it likely that the antibody can work on dog tissues as well?
M. Li
Verified customer
Asked: 2019-07-10
Answer
The anti-Hsp70/HSPA1A/HSPA1B antibody (PA1813) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-10
Question
I see that the anti-Hsp70/HSPA1A/HSPA1B antibody PA1813 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-06-19
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-06-19
Question
We have seen staining in rat cajal-retzius cell fetal brain cortex. Do you have any suggestions? Is anti-Hsp70/HSPA1A/HSPA1B antibody supposed to stain cajal-retzius cell fetal brain cortex positively?
Verified Customer
Verified customer
Asked: 2018-12-31
Answer
Based on literature cajal-retzius cell fetal brain cortex does express HSPA1A. Based on Uniprot.org, HSPA1A is expressed in endothelial cell, uterus, brain, muscle, pancreas, pns skin, embryonic kidney, brain, cajal-retzius cell fetal brain cortex, cervix carcinoma, cervix carcinoma erythroleukemia, liver, colon carcinoma, among other tissues. Regarding which tissues have HSPA1A expression, here are a few articles citing expression in various tissues:
Brain, Cajal-Retzius cell, and Fetal brain cortex, Pubmed ID: 23349634
Brain, Muscle, Pancreas, PNS, and Skin, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 17081983, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Colon carcinoma, Pubmed ID: 24129315
Liver, Pubmed ID: 24275569
Uterus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2018-12-31
Question
you antibody to test anti-Hsp70/HSPA1A/HSPA1B antibody PA1813 on human colon carcinoma for research purposes, then I may be interested in using anti-Hsp70/HSPA1A/HSPA1B antibody PA1813 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
T. Miller
Verified customer
Asked: 2018-11-12
Answer
The products we sell, including anti-Hsp70/HSPA1A/HSPA1B antibody PA1813, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-11-12
Question
Does PA1813 anti-Hsp70/HSPA1A/HSPA1B antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
A. Johnson
Verified customer
Asked: 2018-09-25
Answer
As indicated on the product datasheet, PA1813 anti-Hsp70/HSPA1A/HSPA1B antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-09-25
Question
Is this PA1813 anti-Hsp70/HSPA1A/HSPA1B antibody reactive to the isotypes of HSPA1A?
Verified Customer
Verified customer
Asked: 2018-09-20
Answer
The immunogen of PA1813 anti-Hsp70/HSPA1A/HSPA1B antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human Hsp70(577-596aa VISWLDANTLAEKDEFEHKR), different from the related mouse sequence by four amino acids and from the related rat sequence by three amino acids. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-09-20
Question
My question regarding product PA1813, anti-Hsp70/HSPA1A/HSPA1B antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-04-18
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1813 anti-Hsp70/HSPA1A/HSPA1B antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-04-18
Question
We bought anti-Hsp70/HSPA1A/HSPA1B antibody for IHC on brain in a previous project. I am using human, and I plan to use the antibody for WB next. We need examining brain as well as cervix carcinoma in our next experiment. Do you have any suggestion on which antibody would work the best for WB?
E. Patel
Verified customer
Asked: 2018-03-30
Answer
I took a look at the website and datasheets of our anti-Hsp70/HSPA1A/HSPA1B antibody and it appears that PA1813 has been validated on human in both IHC and WB. Thus PA1813 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-03-30
Question
Would anti-Hsp70/HSPA1A/HSPA1B antibody PA1813 work for IHC with colon carcinoma?
Verified Customer
Verified customer
Asked: 2017-11-27
Answer
According to the expression profile of colon carcinoma, HSPA1A is highly expressed in colon carcinoma. So, it is likely that anti-Hsp70/HSPA1A/HSPA1B antibody PA1813 will work for IHC with colon carcinoma.
Boster Scientific Support
Answered: 2017-11-27
Question
I was wanting to use your anti-Hsp70/HSPA1A/HSPA1B antibody for IHC for human colon carcinoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human colon carcinoma identification?
Verified Customer
Verified customer
Asked: 2017-10-26
Answer
You can see on the product datasheet, PA1813 anti-Hsp70/HSPA1A/HSPA1B antibody has been validated for IHC, ICC, WB on human, rat tissues. We have an innovator award program that if you test this antibody and show it works in human colon carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-10-26
Question
Will anti-Hsp70/HSPA1A/HSPA1B antibody PA1813 work on dog ICC with uterus?
M. Zhang
Verified customer
Asked: 2015-02-20
Answer
Our lab technicians have not tested anti-Hsp70/HSPA1A/HSPA1B antibody PA1813 on dog. You can run a BLAST between dog and the immunogen sequence of anti-Hsp70/HSPA1A/HSPA1B antibody PA1813 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog uterus in ICC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-02-20
Question
Please see the WB image, lot number and protocol we used for colon carcinoma using anti-Hsp70/HSPA1A/HSPA1B antibody PA1813. Please let me know if you require anything else.
M. Thomas
Verified customer
Asked: 2013-10-30
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-10-30
