Anti-Hsp70/HSPA1A/HSPA1B Antibody

Boster Bio Anti-Hsp70/HSPA1A/HSPA1B Antibody catalog # PA1214. Tested in Flow Cytometry, IF, IHC-P, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 6 publication(s).

Product Info Summary

SKU: PA1214
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IF, IHC-P, IHC-F, ICC, WB

Product Name

Anti-Hsp70/HSPA1A/HSPA1B Antibody

See all HSPA1B products

SKU/Catalog Number

PA1214

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Hsp70/HSPA1A/HSPA1B Antibody catalog # PA1214. Tested in Flow Cytometry, IF, IHC-P, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Hsp70/HSPA1A/HSPA1B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1214)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human Hsp70 (13-31aa TTYSCVGVFQHGKVEIIAN), identical to the related rat and mouse sequence.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PA1214 is reactive to Hspa1b in Human, Mouse, Rat

Applications

PA1214 is guaranteed for Flow Cytometry, IF, IHC-P, IHC-F, ICC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Mouse, Rat
Immunocytochemistry , 0.5-1μg/ml, Human
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human

Validation Images & Assay Conditions

Gene/Protein Information For Hspa1b (Source: Uniprot.Org, NCBI)

Uniprot ID

P0DMV9

Gene ID

3304

Gene Name

Hspa1b

Full Name

Heat shock 70 kDa protein 1B

Weight

70.052kDa

Superfamily

heat shock protein 70 family

Alternative Names

FLJ54328; Heat shock 70 kDa protein 1/2; heat shock 70 kDa protein 1A/1B; heat shock 70kD protein 1B; heat shock 70kDa protein 1B; HSP70.1/HSP70.2; HSP70-1/HSP70-2; HSP70-1B; HSP70-2; HSPA1; HSPA1A HSPA1B HSP70-1, HSP70-1B, HSP70-2, HSP70.1, HSP70.2, HSP72, HSPA1, HSX70 heat shock protein family A (Hsp70) member 1B heat shock 70 kDa protein 1B|HSP70-1/HSP70-2|HSP70.1/HSP70.2|Heat shock 70 kDa protein 1|Heat shock 70 kDa protein 1A|heat shock 70 kDa protein 1/2|heat shock 70 kDa protein 1A/1B|heat shock 70 kDa protein 2|heat shock 70kD protein 1B|heat shock 70kDa protein 1B

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Hspa1b, check out the Hspa1b Infographic

Hspa1b infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Hspa1b: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

PA1214 has been cited in 6 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Pan W,Liang J,Tang H,Fang X,Wang F,Ding Y,Huang H,Zhang H.Differentially expressed microRNA profiles in exosomes from vascular smooth muscle cells associated with coronary artery calcification.Int J Biochem Cell Biol.2020 Jan;118:105645.doi:10.1016/j.bioc
Species: Mouse
PA1214 usage in article: APP:WB, SAMPLE:MOVAS-1 CELL, DILUTION:NA

Therapeutic efficacy of trehalose eye drops for treatment of murine dry eye induced by an intelligently controlled environmental system

Down-modulation of heat shock protein 70 and up-modulation of Caspase-3 during schisandrin B-induced apoptosis in human hepatoma SMMC-7721 cells

Effects of intracerebral hemorrhage and subsequent minimally invasive hematoma aspiration on expression of apoptosisrelated genes in rats.

Effects of minocycline on the expression of NGF and HSP70 and its neuroprotection role following intracerebral hemorrhage in rats

Yi Sx, Peng Y, Chang Xr, Peng N, Yan J, Lin Yp. World J Gastroenterol. 2007 Apr 21;13(15):2174-8. Effect Of Pre-Moxibustion On Apoptosis And Proliferation Of Gastric Mucosa Cells.

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17 Customer Q&As for Anti-Hsp70/HSPA1A/HSPA1B Antibody

Question

I see that the anti-Hsp70/HSPA1A/HSPA1B antibody PA1214 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2020-04-10

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2020-04-10

Question

Is this PA1214 anti-Hsp70/HSPA1A/HSPA1B antibody reactive to the isotypes of HSPA8?

K. Moore

Verified customer

Asked: 2020-04-02

Answer

The immunogen of PA1214 anti-Hsp70/HSPA1A/HSPA1B antibody is A synthetic peptide corresponding to a sequence at the N-terminus of human Hsp70(13-31aa TTYSCVGVFQHGKVEIIAN), identical to the related rat and mouse sequence. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2020-04-02

Question

I would like to test anti-Hsp70/HSPA1A/HSPA1B antibody PA1214 on rat colon carcinoma ovarian carcinoma for research purposes, then I may be interested in using anti-Hsp70/HSPA1A/HSPA1B antibody PA1214 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2020-03-27

Answer

The products we sell, including anti-Hsp70/HSPA1A/HSPA1B antibody PA1214, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2020-03-27

Question

Please see the WB image, lot number and protocol we used for colon carcinoma ovarian carcinoma using anti-Hsp70/HSPA1A/HSPA1B antibody PA1214. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2020-02-13

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-02-13

Question

We are currently using anti-Hsp70/HSPA1A/HSPA1B antibody PA1214 for rat tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on feline tissues as well?

Verified Customer

Verified customer

Asked: 2020-01-08

Answer

The anti-Hsp70/HSPA1A/HSPA1B antibody (PA1214) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-01-08

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for colon carcinoma ovarian carcinoma using anti-Hsp70/HSPA1A/HSPA1B antibody PA1214. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-12-10

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-12-10

Question

Is there a BSA free version of anti-Hsp70/HSPA1A/HSPA1B antibody PA1214 available?

Verified Customer

Verified customer

Asked: 2019-11-14

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Hsp70/HSPA1A/HSPA1B antibody PA1214 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-11-14

Question

Does PA1214 anti-Hsp70/HSPA1A/HSPA1B antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-10-11

Answer

You can see on the product datasheet, PA1214 anti-Hsp70/HSPA1A/HSPA1B antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-10-11

Question

Is a blocking peptide available for product anti-Hsp70/HSPA1A/HSPA1B antibody (PA1214)?

Verified Customer

Verified customer

Asked: 2019-07-16

Answer

We do provide the blocking peptide for product anti-Hsp70/HSPA1A/HSPA1B antibody (PA1214). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-07-16

Question

Is the antigen retrieval method for PA1214 PIER or HIER?

Verified customer

Asked: 2019-06-27

Answer

The antigen retrieval method is HIER for the ICC images of the Anti-Hsp70/HSPA1A/HSPA1B Antibody (PA1214).

Boster Scientific Support

Answered: 2019-06-28

Question

My team were happy with the WB result of your anti-Hsp70/HSPA1A/HSPA1B antibody. However we have observed positive staining in lymphoblast nucleolus. using this antibody. Is that expected? Could you tell me where is HSPA8 supposed to be expressed?

K. Johnson

Verified customer

Asked: 2019-02-26

Answer

According to literature, lymphoblast does express HSPA8. Generally HSPA8 expresses in cytoplasm. melanosome. nucleus, nucleolus. Regarding which tissues have HSPA8 expression, here are a few articles citing expression in various tissues:
Brain, Cajal-Retzius cell, and Fetal brain cortex, Pubmed ID: 1286667
Cervix carcinoma, Pubmed ID: 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Colon carcinoma, Pubmed ID: 24129315
Embryonic kidney, Pubmed ID: 17525332
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Lymphoblast, Pubmed ID: 14654843
Melanoma, Pubmed ID: 17081065
Placenta, and Skin, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2019-02-26

Question

Does anti-Hsp70/HSPA1A/HSPA1B antibody PA1214 work for WB with colon carcinoma ovarian carcinoma?

Verified Customer

Verified customer

Asked: 2018-08-31

Answer

According to the expression profile of colon carcinoma ovarian carcinoma, HSPA8 is highly expressed in colon carcinoma ovarian carcinoma. So, it is likely that anti-Hsp70/HSPA1A/HSPA1B antibody PA1214 will work for WB with colon carcinoma ovarian carcinoma.

Boster Scientific Support

Answered: 2018-08-31

Question

We have been able to see staining in rat lymphoblast. Are there any suggestions? Is anti-Hsp70/HSPA1A/HSPA1B antibody supposed to stain lymphoblast positively?

Verified Customer

Verified customer

Asked: 2018-08-13

Answer

From literature lymphoblast does express HSPA8. From Uniprot.org, HSPA8 is expressed in frontal cortex, placenta skin, embryonic kidney, colon carcinoma ovarian carcinoma, brain, cajal-retzius cell fetal brain cortex, lymphoblast, melanoma, leukemic t-cell, cervix carcinoma, cervix carcinoma erythroleukemia, liver, colon carcinoma, among other tissues. Regarding which tissues have HSPA8 expression, here are a few articles citing expression in various tissues:
Brain, Cajal-Retzius cell, and Fetal brain cortex, Pubmed ID: 1286667
Cervix carcinoma, Pubmed ID: 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Colon carcinoma, Pubmed ID: 24129315
Embryonic kidney, Pubmed ID: 17525332
Leukemic T-cell, Pubmed ID: 19690332
Liver, Pubmed ID: 24275569
Lymphoblast, Pubmed ID: 14654843
Melanoma, Pubmed ID: 17081065
Placenta, and Skin, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2018-08-13

Question

I was wanting to use your anti-Hsp70/HSPA1A/HSPA1B antibody for WB for rat colon carcinoma ovarian carcinoma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat colon carcinoma ovarian carcinoma identification?

Verified Customer

Verified customer

Asked: 2018-07-30

Answer

As indicated on the product datasheet, PA1214 anti-Hsp70/HSPA1A/HSPA1B antibody has been tested for IHC, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat colon carcinoma ovarian carcinoma in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-07-30

Question

I have a question about product PA1214, anti-Hsp70/HSPA1A/HSPA1B antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2017-10-09

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1214 anti-Hsp70/HSPA1A/HSPA1B antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2017-10-09

Question

Our lab used your anti-Hsp70/HSPA1A/HSPA1B antibody for IHC on brain a few years ago. I am using mouse, and We want to use the antibody for WB next. I was wanting to use examining brain as well as placenta skin in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?

T. Banerjee

Verified customer

Asked: 2015-06-16

Answer

I have checked the website and datasheets of our anti-Hsp70/HSPA1A/HSPA1B antibody and it appears that PA1214 has been tested on mouse in both IHC and WB. Thus PA1214 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in mouse even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2015-06-16

Question

Does anti-Hsp70/HSPA1A/HSPA1B antibody PA1214 work on dog IHC with cervix carcinoma?

E. Banerjee

Verified customer

Asked: 2013-07-08

Answer

Our lab technicians have not tested anti-Hsp70/HSPA1A/HSPA1B antibody PA1214 on dog. You can run a BLAST between dog and the immunogen sequence of anti-Hsp70/HSPA1A/HSPA1B antibody PA1214 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated dog samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in dog cervix carcinoma in IHC, you can get your next antibody for free.

Boster Scientific Support

Answered: 2013-07-08

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Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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