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Hspa1b antibody (PA1214) performs well in Flow Cytometry, IF, IHC, IHC-F, ICC, WB and shows reactivity with Human, Mouse, Rat.

Validated for inter-lot consistency, specificity, and reproducibility
Committed to quality since 1993
Over 25 Publications

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Flow Cytometry analysis of 293T cells using anti-Hsp70 antibody (PA1214).
Overlay histogram showing 293T cells stained with PA1214 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Hsp70 Antibody (PA1214,1μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x10⁶) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.

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Anti-Hsp70/HSPA1A/HSPA1B Antibody Picoband®

Hspa1b antibody

25 Publications

Reactive Species	Host	Application
Human, Mouse, Rat	Rabbit	Flow Cytometry, IF, IHC, IHC-F, ICC, WB

Tested Applications

Hspa1b antibody was positively detected by WB in:

human HEK293 whole cell, human Caco-2 whole cell, human PC-3 whole cell, human THP-1 whole cell, human U2OS whole cell, rat brain tissue, rat liver tissue, rat spleen tissue, rat PC-12 whole cell, mouse brain tissue, mouse liver tissue, mouse spleen tissue, mouse RAW2467 whole cell

Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane

Hspa1b antibody was positively detected by IHC in:

rat brain tissue, mammary cancer tissue

Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.

Hspa1b antibody was positively detected by IHC-F in:

rat intestine tissue

Hspa1b antibody was positively detected by ICC/IF in:

Hela cell

Hspa1b antibody was positively detected by ICC in:

HELA cell

Hspa1b antibody was positively detected by FC in:

293T cell

Reactive Data

Boster Guarantee: All Tested Recommend Predicted species What is this?

IHC

Human

Tested

Rat

Tested

Recommend

Mouse

Tested

Product Information

Product Name

Anti-Hsp70/HSPA1A/HSPA1B Antibody Picoband®
View all HSPA1B Antibodies

Form

Lyophilized

Uniprot

P0DMV8/P0DMV9

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Host

Rabbit

Contents

Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human Hsp70, identical to the related rat and mouse sequence.

Cross-Reactivity

No cross-reactivity with other proteins

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

70 kDa

Background of Hspa1b

The 70 kilodalton heat shock proteins (Hsp70s) are a family of ubiquitously expressed heat shock proteins. The Hsp70s are an important part of the cell's machinery for protein folding, and help to protect cells from stress. All of the Hsp70 proteins have three major functional domains: An N-terminal ATPase domain binds ATP (Adenosine triphosphate) and hydrolyzes it to ADP (Adenosine diphosphate); A substrate binding domain contains a groove with an affinity for neutral, hydrophobic amino acid residues; A C-terminal domain rich in alpha helical structure acts as a 'lid' for the substrate binding domain. By binding tightly to partially-synthesized peptide sequences (incomplete proteins), Hsp70 prevents them from aggregating and being rendered nonfunctional. And it also can act to protect cells from thermal or oxidative stress. Finally, Hsp70 seems to be able to participate in disposal of damaged or defective proteins. Interaction with CHIP (Carboxyl-terminus of Hsp70 Interacting Protein)–an E3 ubiquitin ligase–allows Hsp70 to pass proteins to the cell's ubiquitination and proteolysis pathways.

Cite This Product

Anti-Hsp70/HSPA1A/HSPA1B Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1214)

Protocols

Western Blot protocol for Hspa1b antibody

Review Protocol

Immunohistochemistry (IHC) protocol for Hspa1b antibody

Review Protocol

Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for Hspa1b antibody

Review Protocol

Immunocytochemistry (ICC) protocol for Hspa1b antibody

Review Protocol

Flow Cytometry (FC) protocol for Hspa1b antibody

Review Protocol

Publications

Different concentrations of 2-Undecanone triggers repellent and nematicidal responses in Caenorhabditis elegans

Published: 2025-04-23

Journal: Scientific Reports

Application: WB

Impact Factor: 3.90

Species: C. elegans

PMID: 40269070

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Anti-Hsp70/HSPA1A/HSPA1B Antibody Picoband®

Hspa1b antibody

Overview

Tested Applications

Recommend Dilution

Reactive Data

Product Information

Protocols

Publications

Different concentrations of 2-Undecanone triggers repellent and nematicidal responses in Caenorhabditis elegans

A multifunctional ‘golden cicada’ nanoplatform breaks the thermoresistance barrier to launch cascade augmented synergistic effects of photothermal/gene therapy

Aspirin relieves the calcification of aortic smooth muscle cells by enhancing the heat shock response

Germacrone Regulates HBXIP-Mediated Cell Cycle, Apoptosis and Promotes the Formation of Autophagosomes to Inhibit the Proliferation of Gastric Cancer Cells

CircWHSC1 promotes ovarian cancer progression by regulating MUC1 and hTERT through sponging miR-145 and miR-1182

Effects of osmotic stress on Na+/K+-ATPase, caspase 3/7 activity, and the expression profiling of sirt1, hsf1, and hsp70 in the roughskin sculpin (Trachidermus fasciatus)

ROS-induced HSP70 promotes cytoplasmic translocation of high-mobility group box 1b and stimulates antiviral autophagy in grass carp kidney cells

Heat shock protein 70 protects mouse against post-infection irritable bowel syndrome via up-regulating intestinal γδ T cell’s Th17 response

Evaluation of short- and long-term efficacy of chemoradiotherapy for advanced cervical cancer using HSP70 protein combined with multimodal MRI

Chondroprotective Effects and Multitarget Mechanisms of Fu Yuan Capsule in a Rat Osteoarthritis Model

Effects of intracerebral hemorrhage and subsequent minimally invasive hematoma aspiration on expression of apoptosisrelated genes in rats

Study on the immune response to recombinant Hsp70 protein from Megalobrama amblycephala

Changes of ultrastructure and downregulation of heat shock protein 70 and fibroblast growth factor 2 in gastric mucosa of rats with depressive-like behaviour

Molecular mechanism of polypeptides from Chlamys farreri (PCF)’s anti-apoptotic effect in UVA-exposed HaCaT cells involves HSF1/HSP70, JNK, XO, iNOS and NO/ROS

Effect of moxibustion treatment on cell apoptosis and expressions of heat shock protein and second mitochondrial activator of caspase in acute gastric mucosal lesion of rats

Therapeutic efficacy of trehalose eye drops for treatment of murine dry eye induced by an intelligently controlled environmental system

Effects of exogenous human leptin on heat shock protein 70 expression in MCF-7 breast cancer cells and breast carcinoma of nude mice xenograft model

Effects of adrenergic agents on stress-induced brain microstructural and immunochemical changes in adult male Wistar rats

Antioxidant responses of Chilo suppressalis (Lepidoptera: Pyralidae) larvae exposed to thermal stress

A nuclear ligand MRG15 involved in the proapoptotic activity of medicinal fungal galectin AAL (Agrocybe aegerita lectin)

Serum proteome analysis for profiling protein markers associated with carcinogenesis and lymph node metastasis in nasopharyngeal carcinoma

Association of high expression in rat gastric mucosal heat shock protein 70 induced by moxibustion pretreatment with protection against stress injury

Effect of pre-moxibustion on apoptosis and proliferation of gastric mucosa cells

Expression of Hsp70 in Acute Lung Injury Induced by Lipopolysaccharide

Impairment of redox state and dopamine level induced by α-synuclein aggregation and the prevention effect of hsp70

Review

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