Human A1BG/alpha 1B-Glycoprotein ELISA Kit PicoKine™

Human A1BG/alpha 1B-Glycoprotein ELISA Kit PicoKine™ (96 Tests). Quantitate Human A1BG in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. Cited in 1 publication(s).

Product Info Summary

SKU: EK1488
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Human A1BG/alpha 1B-Glycoprotein ELISA Kit PicoKine™

See all alpha 1B-Glycoprotein products

SKU/Catalog Number

EK1488

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human A1BG/alpha 1B-Glycoprotein ELISA Kit PicoKine™ (96 Tests). Quantitate Human A1BG in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human A1BG/alpha 1B-Glycoprotein ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1488)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: A22-S495

Sensitivity

<10 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1488 is reactive to A1BG in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK1488 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK1488-CAP Anti-Human A1BG Pre-coated 96-well strip microplate 1
EK1488-ST Human A1BG Standard 2 vials, 10 ng/tube
EK1488-DA Human A1BG Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631262512502500500010000
O.D.0.1060.1210.1560.2260.4040.6921.1252.009

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect alpha 1B-Glycoprotein, Dilution ratio of 1:100000, concentration in serum is around 100ug/ml.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human A1BG/alpha 1B-Glycoprotein ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)2361951560725618695510
Standard deviation15.8185.84409.3120.4889.71495.9
CV (%)6.7%4.4%7.3%8%4.8%9%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of alpha 1B-Glycoprotein in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 123625625823124511.94.8%
Sample 219511726164616701748120.66.8%
Sample 356075964620663016019267.984.4%
*number of samples for each test n=16.

Gene/Protein Information For A1BG (Source: Uniprot.Org, NCBI)

Uniprot ID

P04217

Gene ID

1

Gene Name

A1BG

Full Name

Alpha-1B-glycoprotein

Weight

54.254kDa

Alternative Names

A1B; A1BG; ABG; alpha 1BGlycoprotein; alpha 1B-Glycoprotein; GAB A1BG A1B, ABG, GAB, HYST2477 alpha-1-B glycoprotein alpha-1B-glycoprotein|HEL-S-163pA|epididymis secretory sperm binding protein Li 163pA

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on A1BG, check out the A1BG Infographic

A1BG infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for A1BG: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK1488 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Wang, S., Zhang, J., Meng, F. J., Yan, Y. J., Wang, B., & Guan, Z. Y. (2020). Combination of pembrolizumab and 125I attenuates the aggressiveness of non‑small cell lung cancer. Oncology Letters, 19(6), 4142-4150.

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7 Customer Q&As for Human A1BG/alpha 1B-Glycoprotein ELISA Kit PicoKine™

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2020-09-24

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2020-09-24

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2019-04-13

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2019-04-13

Question

Q: What is the optimal O.D. value for alpha 1B-Glycoprotein ELISA kit? I used your alpha 1B-Glycoprotein ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain alpha 1B-Glycoprotein even though the O.D. values are not very high?

Verified Customer

Verified customer

Asked: 2019-04-04

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the example mentioned, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this alpha 1B-Glycoprotein ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of alpha 1B-Glycoprotein that can be declared/interpreted as positive by the above standard is 10pg/ml.

Boster Scientific Support

Answered: 2019-04-04

Question

Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?

J. Varma

Verified customer

Asked: 2019-01-08

Answer

A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer may affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2019-01-08

Question

Q: how to analyze ELISA data? I have performed alpha 1B-Glycoprotein level in serum.

S. Hernandez

Verified customer

Asked: 2018-01-23

Answer

A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2018-01-23

Question

Q: is it okay to use citrate plasma as samples in Human alpha 1B-Glycoprotein Picokine® ELISA Kit (Catalog # EK1488)?

Verified Customer

Verified customer

Asked: 2015-09-23

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of alpha 1B-Glycoprotein causing disruption of its protein structure. alpha 1B-Glycoprotein may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the alpha 1B-Glycoprotein ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2015-09-23

Question

Q: how to thaw whole blood sample for alpha 1B-Glycoprotein ELISA after freezing?

W. Sunder

Verified customer

Asked: 2014-06-22

Answer

A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test alpha 1B-Glycoprotein for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.

Boster Scientific Support

Answered: 2014-06-22

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