Human BAFF / TNFSF13B ELISA Kit PicoKine™

Human BAFF / TNFSF13B ELISA Kit PicoKine™ (96 Tests). Quantitate Human TNFSF13B in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 2pg/ml. Cited in 4 publication(s).

Product Info Summary

SKU: EK0663
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Human BAFF / TNFSF13B ELISA Kit PicoKine™

See all BAFF/BLyS/TNFSF13B products

SKU/Catalog Number

EK0663

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human BAFF / TNFSF13B ELISA Kit PicoKine™ (96 Tests). Quantitate Human TNFSF13B in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 2pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human BAFF / TNFSF13B ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0663)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: A134-L285

Sensitivity

<2 pg/ml

Assay Range

62.5 pg/ml - 4,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0663 is reactive to TNFSF13B in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK0663 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0663-CAP Anti-Human TNFSF13B Pre-coated 96-well strip microplate 1
EK0663-ST Human TNFSF13B Standard 2 vials, 10 ng/tube
EK0663-DA Human TNFSF13B Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)062.5125250500100020004000
O.D.0.0380.0720.1210.1790.3110.5480.9351.829

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect BAFF/BLyS/TNFSF13B, Dilution ratio of 1:4, concentration in serum is around 4ng/ml.

Some articles we found to cite concentrations of BAFF/BLyS/TNFSF13B in samples: 25453636, 24906685 (Pubmed IDs).

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human BAFF / TNFSF13B ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)12752212291245481268
Standard deviation5.4638.6279.885.747.67103.97
CV (%)4.3%7.4%6.5%4.6%8.7%8.2%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of BAFF/BLyS/TNFSF13B in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 11271251491271329.847.4%
Sample 25225135365285248.41.6%
Sample 31229108512151223118859.675%
*number of samples for each test n=16.

Gene/Protein Information For TNFSF13B (Source: Uniprot.Org, NCBI)

Uniprot ID

Q9Y275

Gene ID

10673

Gene Name

TNFSF13B

Full Name

Tumor necrosis factor ligand superfamily member 13B

Weight

31.223kDa

Superfamily

tumor necrosis factor family

Alternative Names

ApoL related ligand TALL-1; B lymphocyte stimulator; BAFF; BAFFB-cell activating factor; B-cell-activating factor; BLyS; BLYSB-lymphocyte stimulator; CD257 antigen; CD257; Dendritic cell-derived TNF-like molecule; DTL; TALL1; TALL-1delta BAFF; TALL1Delta4 BAFF; THANK; TNF- and APOL-related leukocyte expressed ligand 1; TNF and ApoL-related leukocyte expressed ligand 1; TNF homolog that activates apoptosis; TNFSF13B; TNFSF20; tumor necrosis factor (ligand) superfamily, member 13b; tumor necrosis factor (ligand) superfamily, member 20; tumor necrosis factor ligand superfamily member 13B; tumor n TNFSF13B BAFF, BLYS, CD257, DTL, TALL-1, TALL1, THANK, TNFSF20, TNLG7A, ZTNF4 TNF superfamily member 13b tumor necrosis factor ligand superfamily member 13B|ApoL related ligand TALL-1|B-cell-activating factor|B-lymphocyte stimulator|Delta4 BAFF|TNF and ApoL-related leukocyte expressed ligand 1|TNF homolog that activates apoptosis|delta BAFF|dendritic cell-derived TNF-like molecule|epididymis secretory sperm binding protein|tumor necrosis factor (ligand) superfamily, member 13b|tumor necrosis factor (ligand) superfamily, member 20|tumor necrosis factor ligand 7A|tumor necrosis factor superfamily member 13b|tumor necrosis factor-like protein ZTNF4

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on TNFSF13B, check out the TNFSF13B Infographic

TNFSF13B infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for TNFSF13B: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0663 has been cited in 4 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

AB0018 B cell disturbance in rheumatoid arthritis patients: comparative study between treated and non-treated patients
Species: Human

Prognostic Significance of Serum BAFF, APRIL, TACI and BCMA Levels in Chronic Lymphocytic Leukemia

Pretransplant serum BAFF levels are associated with pretransplant HLA immunization and renal allograft survival

G??zmen S, Karapnar Th, T??fek??i O, Vergin C, Y??ksel F, Irken G, Oren H. Blood Coagul Fibrinolysis. 2014 Jun 6. [Epub Ahead Of Print] B-Cell-Activating Factor, A Proliferation Inducing Ligand And Co-Stimulatory Molecules In The Pathogenesis Of I...

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9 Customer Q&As for Human BAFF / TNFSF13B ELISA Kit PicoKine™

Question

Q: if the enzyme conjugated BAFF antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the BAFF antigen?

M. Walker

Verified customer

Asked: 2020-11-23

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2020-11-23

Question

To Boster Support: How this EK0663 antibody has been purified? By ammonium sulfate precipitation?

Verified Customer

Verified customer

Asked: 2020-05-08

Answer

Yes, this EK0663 antibody was purified by ammonium sulfate precipitation and is tested by Western blots.

Boster Scientific Support

Answered: 2020-05-08

Question

I want to purchase Human BAFF / TNFSF13B ELISA Kit, but I do not have any idea which sequence of the epitope in BAFFr. My protein is mutated and I wonder if this antibody Human BAFF / TNFSF13B ELISA Kit can be used to detect my protein BAFFr. Could you tell me which amino acid sequence of the Epitope in human BAFFr?

Verified Customer

Verified customer

Asked: 2020-03-31

Answer

The peptide sequence of Human BAFF / TNFSF13B ELISA Kit are GSTELVTTKTAGPEQ (amino acids 169-183 of Human Baff receptor). Also you can check more information in our catalogue.

Boster Scientific Support

Answered: 2020-03-31

Question

Q: how to thaw whole blood sample for BAFF ELISA after freezing?

Verified Customer

Verified customer

Asked: 2020-01-04

Answer

A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test BAFF for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2020-01-04

Question

Q: is it okay to use citrate plasma as samples in Human BAFF Picokine® ELISA Kit (Catalog # EK0663)?

Verified Customer

Verified customer

Asked: 2019-10-04

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of BAFF causing degradation of its protein structure. BAFF may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the BAFF ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2019-10-04

Question

Was baculovirus used to produce the standard of EK0663?

Verified Customer

Verified customer

Asked: 2019-06-21

Answer

We didn't use baculovirus to produce the standard of EK0663.

Boster Scientific Support

Answered: 2019-06-21

Question

What is the chemical name and concentration of acid contained in the TMB stop solution?

Verified Customer

Verified customer

Asked: 2019-04-17

Answer

Our TMB stop solution contains 2N sulphuric acid.

Boster Scientific Support

Answered: 2019-04-17

Question

Q: What is the optimal O.D. value for BAFF ELISA kit? I used your BAFF ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain BAFF even though the O.D. values are not very high?

M. Dhawan

Verified customer

Asked: 2018-03-11

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the above example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this BAFF ELISA kit to have sensitivity of 2pg/ml, that means the minimum amount of BAFF that can be declared/interpreted as positive by the above standard is 2pg/ml.

Boster Scientific Support

Answered: 2018-03-11

Question

Are there any preservatives contained in the standard, biotinylated antibody, ABC sample diluent buffer, antibody diluent buffer, and ABC diluent buffer?

Verified Customer

Verified customer

Asked: 2013-12-30

Answer

Our ELISA kits contain 0.02% sodium azide. All of the components, except ABC, TMB color developing reagent, and TMB stop solution, contain 0.02% sodium azide as the preservative.

Boster Scientific Support

Answered: 2013-12-30

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