Click image to see more details
Product Info Summary
| SKU: | EK0663 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human BAFF / TNFSF13B ELISA Kit PicoKine®
SKU/Catalog Number
EK0663
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human BAFF / TNFSF13B ELISA Kit PicoKine® (96 Tests). Quantitate Human TNFSF13B in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 2pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays. BAFF/TNFSF13B is a TNF-superfamily cytokine that binds BAFF-R (TNFRSF13C) and also engages TACI (TNFRSF13B) and BCMA (TNFRSF17), supporting peripheral B-cell maturation/survival and B-cell responses; BAFF-R ligation is linked to non-canonical NF-κB signaling (NIK/TRAF3 axis). Assay context: quantitative sandwich ELISA in human cell culture supernatants/lysates and serum/plasma (heparin, EDTA) (sensitivity 2 pg/mL); cytokine quantitation aligns with common ELISA testing workflow considerations (sample handling, standard-curve QC). Often profiled alongside T-cell costimulatory ligands measured by sCD40L/CD40LG and OX40L/TNFSF4, and with Th2 cytokine IL-4 in immune-activation panels.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human BAFF / TNFSF13B ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0663)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: A134-L285
Capture/Detection Antibodies
The capture antibody is monoclonal antibody from mouse and the detection antibody is polyclonal antibody from goat.
Sensitivity
<2 pg/ml
Assay Range
62.5 pg/ml - 4,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0663 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0663 is reactive to TNFSF13B in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK0663 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of TNFSF13B
BAFF was regularly detected by enzyme-linked immunosorbent assay in brain tissue lysates and in normal spinal fluid, and in astrocytes by double fluorescence microscopy. BAFF was localized in astrocytes close to BAFF-R-expressing immune cells. BAFF receptors were strongly expressed in situ in primary central nervous system (CNS) lymphomas.1 The TNF superfamily member B cell-activating factor (BAFF) plays an important role in humoral immunity and in autoimmune diseases, including RA.Local BAFF gene targeting inhibited proinflammatory cytokine expression, suppressed generation of plasma cells and Th17 cells, and markedly ameliorated joint pathology.2 The B cell activating factor BAFF (BlyS/TALL-1/zTNF4) is a tumor necrosis factor (TNF)-related ligand that promotes B cell survival and binds to three receptors (BCMA, TACI, and the recently described BAFF-R).3 Human BAFF was mapped to chromosome 13q32-34.4 The standard used in this kit is recombinant soluble human BAFF (A134-L295) with the molecular mass of 19.6KDa.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0663-CAP | Anti-Human TNFSF13B Pre-coated 96-well strip microplate | 1 |
| EK0663-ST | Human TNFSF13B Standard | 2 vials, 10 ng/tube |
| EK0663-DA | Human TNFSF13B Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D. | 0.063 | 0.139 | 0.201 | 0.381 | 0.626 | 1.102 | 1.615 | 1.872 |
Data Example Images
Click image to see more details
Human BAFF PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect TNFSF13B, Dilution ratio of 1:4, concentration in serum and plasma is around 4 ng/ml..
Some articles we found to cite concentrations of TNFSF13B in samples: 25453636, 24906685 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human BAFF / TNFSF13B ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 127 | 522 | 1229 | 124 | 548 | 1268 |
| Standard deviation | 5.46 | 38.62 | 79.88 | 5.7 | 47.67 | 103.97 |
| CV (%) | 4.3% | 7.4% | 6.5% | 4.6% | 8.7% | 8.2% |
Specific Publications For Human BAFF / TNFSF13B ELISA Kit PicoKine® (EK0663)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
0 Reviews For Human BAFF / TNFSF13B ELISA Kit PicoKine®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
9 Customer Q&As for Human BAFF / TNFSF13B ELISA Kit PicoKine®
Question
Q: if the enzyme conjugated BAFF antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the BAFF antigen?
M. Walker
Verified customer
Asked: 2020-11-23
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2020-11-23
Question
To Boster Support: How this EK0663 antibody has been purified? By ammonium sulfate precipitation?
Verified Customer
Verified customer
Asked: 2020-05-08
Answer
Yes, this EK0663 antibody was purified by ammonium sulfate precipitation and is tested by Western blots.
Boster Scientific Support
Answered: 2020-05-08
Question
I want to purchase Human BAFF / TNFSF13B ELISA Kit, but I do not have any idea which sequence of the epitope in BAFFr. My protein is mutated and I wonder if this antibody Human BAFF / TNFSF13B ELISA Kit can be used to detect my protein BAFFr. Could you tell me which amino acid sequence of the Epitope in human BAFFr?
Verified Customer
Verified customer
Asked: 2020-03-31
Answer
The peptide sequence of Human BAFF / TNFSF13B ELISA Kit are GSTELVTTKTAGPEQ (amino acids 169-183 of Human Baff receptor). Also you can check more information in our catalogue.
Boster Scientific Support
Answered: 2020-03-31
Question
Q: how to thaw whole blood sample for BAFF ELISA after freezing?
Verified Customer
Verified customer
Asked: 2020-01-04
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test BAFF for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2020-01-04
Question
Q: is it okay to use citrate plasma as samples in Human BAFF Picokine® ELISA Kit (Catalog # EK0663)?
Verified Customer
Verified customer
Asked: 2019-10-04
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of BAFF causing degradation of its protein structure. BAFF may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the BAFF ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2019-10-04
Question
Was baculovirus used to produce the standard of EK0663?
Verified Customer
Verified customer
Asked: 2019-06-21
Answer
We didn't use baculovirus to produce the standard of EK0663.
Boster Scientific Support
Answered: 2019-06-21
Question
What is the chemical name and concentration of acid contained in the TMB stop solution?
Verified Customer
Verified customer
Asked: 2019-04-17
Answer
Our TMB stop solution contains 2N sulphuric acid.
Boster Scientific Support
Answered: 2019-04-17
Question
Q: What is the optimal O.D. value for BAFF ELISA kit? I used your BAFF ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain BAFF even though the O.D. values are not very high?
M. Dhawan
Verified customer
Asked: 2018-03-11
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the above example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this BAFF ELISA kit to have sensitivity of 2pg/ml, that means the minimum amount of BAFF that can be declared/interpreted as positive by the above standard is 2pg/ml.
Boster Scientific Support
Answered: 2018-03-11
Question
Are there any preservatives contained in the standard, biotinylated antibody, ABC sample diluent buffer, antibody diluent buffer, and ABC diluent buffer?
Verified Customer
Verified customer
Asked: 2013-12-30
Answer
Our ELISA kits contain 0.02% sodium azide. All of the components, except ABC, TMB color developing reagent, and TMB stop solution, contain 0.02% sodium azide as the preservative.
Boster Scientific Support
Answered: 2013-12-30
