Introduction
HINT1, also known as Histidine triad nucleotide-binding protein 1 is part of the superfamily named for a near C-terminal HXHXHXX motif (H:Histidine, X:a hydrophobic amino acid) positioned at the ?-phosphate of nucleotide substrates. HINT1 hydrolyzes adenosine 5'-monophosphoramidate substrates such as AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha-acetyl lysine methyl ester and AMP-NH2. Though it was initially considered to be a protein kinase C inhibitor and act as a haplod-insufficient tumor suppressor including spontaneous tumor formation in Hint+/- and Hint-/- , its actual physiologic function is not known.
Overview
| Product Name |
Human TNFSF4/OX40L ELISA Kit PicoKine™
See all OX40 Ligand/TNFSF4 primary antibodies, ELISA kits and proteins |
| SKU/Catalog Number |
EK0857 |
| Storage & Handling |
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.) |
| Description |
Detect Human Ox40L/TNFSF4 with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Ox40L/TNFSF4 tissue specificity: |
| Cite This Product |
Human TNFSF4/OX40L ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0857)
|
Product Specs
| Reactivity/Species |
Human |
| Applications |
ELISA
*Our Boster Guarantee covers the use of this product in the above
tested applications.
**For positive and negative control design, consult "Tissue specificity" under
"Protein Target Info" tab. |
| Sample Type |
cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). |
| Sensitivity |
<10pg/ml |
| Assay Range |
156pg/ml-10000pg/ml |
| Immunogen/Standard |
Expression system for standard: NSO; Immunogen sequence: Q51-L183 |
| Antibody Clonalities |
Capture antibody|Detection antibody
monoclonal antibody from mouse|polyclonal antibody from goat |
| Cross Reactivity |
There is no detectable cross-reactivity with other relevant proteins. |
| Pack Size |
96wells/kit, with removable strips. |
Gene/Protein Basic Information For TNFSF4 (Source: Uniprot.org, NCBI)
| Uniprot Id | P23510 |
|---|
| NCBI Gene Id | 7292 |
|---|
| Species Of This Entry | Human |
|---|
| Gene Name | TNFSF4 |
|---|
| Protein Name | Tumor necrosis factor ligand superfamily member 4 |
|---|
| Superfamily | tumor necrosis factor family |
|---|
| Alternative Names | OX40 Ligand/TNFSF4|CD134 ligand; CD134L; CD252 antigen; CD252; Glycoprotein Gp34; gp34; OX40 antigen ligand; OX40 Ligand; OX40L; OX-40L; OX4OL; TAX transcriptionally-activated glycoprotein 1; tax-transcriptionally activated glycoprotein 1 (34kD); tax-transcriptionally activated glycoprotein 1, 34kD; TNFSF4; tumor necrosis factor (ligand) superfamily, member 4; tumor necrosis factor ligand superfamily member 4; TXGP1 |
|---|
| Molecular Weight | 21050 |
|---|
| Post Tranlational Modifications | Biotinylation; Phosphorylation; Methylation |
|---|
| Subcellular Localization | Membrane; Single-pass type II membrane protein. |
|---|
| Gene Location | 1q25.1, on Chromasome 1, gene sequence: NC_000001.11 (173183729..173462208, complement) |
|---|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For TNFSF4 (Source: Uniprot.org, NCBI)
| Protein Function | Cytokine that binds to TNFRSF4. Co-stimulates T-cell proliferation and cytokine production. |
|---|
| Interacting Proteins | TNFRSF4; Tnfsf4 |
|---|
| Related Diseases | Inflammation; Autoimmune Reaction; Autoimmune Diseases; Lupus Erythematosus, Systemic; Neoplasms; Malignant Neoplasms; Atherosclerosis; Arthritis; Autoimmunity; Rheumatoid Arthritis; Intestinal Diseases; Infarction; Asthma; Pneumonia; Leukemia; Myocardial Infarction; Sclerosis; Colitis; Carcinoma; Diffuse Scleroderma |
|---|
| Related Pathways | Cell Activation; Pathogenesis; T Cell Activation; Secretion; Immune Response; Cell Proliferation; Cytokine Production; T Cell Proliferation; Cell Development; Cytokine Secretion; Cell Differentiation; Hypersensitivity; Cell Death; B Cell Activation; Sensitization; Regulation Of Gene Expression; Cell Adhesion; Cell Migration; Cell Division; Cell Motility |
|---|
| Background | OX40L is the ligand for CD134 and is expressed on such cells as DC2s enabling amplification of Th2 cell differentiation. OX40L has also been designated CD252. By analysis of an interspecific backcross, the mouse Tnfsf4 gene is mapped to chromosome 1. Using fluorescence in situ hybridization, they localized the human TNFSF4 gene to 1q25, a region sharing homology of synteny with the portion of mouse chromosome 1 containing the Tnfsf4 gene. OX40L strongly inhibited the generation of IL-10-producing Tr1 cells induced by two physiologic stimuli, the inducible costimulatory ligand and immature dendritic cells. In addition, OX40L strongly inhibited IL-10 production and suppressive function of differentiated IL-10-producing Tr1 cells. Tnfsf4 underlies Ath1 in mice and that polymorphisms in its human homolog TNFSF4 increase the risk of myocardial infarction in humans. |
|---|
| Scientific References | PMID: 1996093 by Miura S., et al. Molecular cloning and characterization of a novel glycoprotein, gp34, that is specifically induced by the human T-cell leukemia virus type I transactivator p40tax.
PMID: 7913952 by Godfrey W.R., et al. Identification of a human OX-40 ligand, a costimulator of CD4+ T cells with homology to tumor necrosis factor. |
|---|
Kit Components And Assay QC Data Details Of Human TNFSF4/OX40L ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
| Catalog number | Description | Quantity |
| EK0857-CAP | 96-well plate precoated with anti-Human OX40 Ligand/TNFSF4 antibody | 1 |
| EK0857-ST | lyophilized recombinant Human OX40 Ligand/TNFSF4 standard | 10ng/tube |
| EK0857-DA | biotinylated anti-Human OX40 Ligand/TNFSF4 antibody | 130ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 130ul |
| AR1106-1 | sample diluent buffer | 30ml |
| AR1106-2 | antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EK0857 Human TNFSF4/OX40L ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
| Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.056 | 0.138 | 0.238 | 0.365 | 0.657 | 1.151 | 1.648 | 2.230 |
We measured random samples of Human TNFSF4/OX40L ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision |
|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 209 | 1225 | 4641 | 228 | 1277 | 4885 |
| Standard deviation | 8.56 | 51.45 | 305.70 | 12.54 | 72.78 | 365.77 |
| CV(%) | 4.1% | 4.2% | 6.6% | 5.5% | 5.7% | 7.5% |
We measured the performance consistency of Human TNFSF4/OX40L ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|
| Sample 1 | 209 | 197 | 197 | 202 | 201 | 4.91 | 2.4% |
| Sample 2 | 1225 | 1132 | 1200 | 1122 | 1169 | 43.79 | 3.7% |
| Sample 3 | 4641 | 5209 | 4997 | 5343 | 5047 | 265.13 | 5.2% |
*number of samples for each test n=16.
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
See all OX40 Ligand/TNFSF4 primary antibodies,
Other Recommended Resources
Here are featured tools and databases that you might find useful.
Total number of citations: 0
