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Product Info Summary
| SKU: | EK0857 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
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Product info
Product Name
Human TNFSF4/OX40L ELISA Kit PicoKine®
View all OX40 Ligand/TNFSF4 ELISA kits
SKU/Catalog Number
EK0857
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human TNFSF4/OX40L ELISA Kit PicoKine™ (96 Tests). Quantitate Human TNFSF4 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human TNFSF4/OX40L ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0857)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: Q51-L183
Sensitivity
<10 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0857 is reactive to TNFSF4 in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK0857 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of OX40 Ligand/TNFSF4
OX40L is the ligand for CD134 and is expressed on such cells as DC2s enabling amplification of Th2 cell differentiation. OX40L has also been designated CD252. By analysis of an interspecific backcross, the mouse Tnfsf4 gene is mapped to chromosome 1. Using fluorescence in situ hybridization, they localized the human TNFSF4 gene to 1q25, a region sharing homology of synteny with the portion of mouse chromosome 1 containing the Tnfsf4 gene. OX40L strongly inhibited the generation of IL-10-producing Tr1 cells induced by two physiologic stimuli, the inducible costimulatory ligand and immature dendritic cells. In addition, OX40L strongly inhibited IL-10 production and suppressive function of differentiated IL-10-producing Tr1 cells. Tnfsf4 underlies Ath1 in mice and that polymorphisms in its human homolog TNFSF4 increase the risk of myocardial infarction in humans.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0857-CAP | Anti-Human TNFSF4 Pre-coated 96-well strip microplate | 1 |
| EK0857-ST | Human TNFSF4 Standard | 2 vials, 10 ng/tube |
| EK0857-DA | Human TNFSF4 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.056 | 0.138 | 0.238 | 0.365 | 0.657 | 1.151 | 1.548 | 2.030 |
Data Example Images
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Human TNFSF4/OX40L PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect OX40 Ligand/TNFSF4, Dilution ratio of 1:1, concentration in serum is target in serum is below detection limit..
Some articles we found to cite concentrations of OX40 Ligand/TNFSF4 in samples: 18843780 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human TNFSF4/OX40L ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 209 | 1225 | 4641 | 228 | 1277 | 4885 |
| Standard deviation | 8.56 | 51.45 | 305.70 | 12.54 | 72.78 | 365.77 |
| CV (%) | 4.1% | 4.2% | 6.6% | 5.5% | 5.7% | 7.5% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of OX40 Ligand/TNFSF4 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 209 | 197 | 197 | 202 | 201 | 4.91 | 2.4% |
| Sample 2 | 1225 | 1132 | 1200 | 1122 | 1169 | 43.79 | 3.7% |
| Sample 3 | 4641 | 5209 | 4997 | 5343 | 5047 | 265.13 | 5.2% |
Protein Target Info & Infographic
Gene/Protein Information For TNFSF4 (Source: Uniprot.Org, NCBI)
Uniprot ID
P23510
Gene ID
7292
Gene Name
TNFSF4
Full Name
Tumor necrosis factor ligand superfamily member 4
Weight
21.05kDa
Superfamily
tumor necrosis factor family
Alternative Names
CD134 ligand; CD134L; CD252 antigen; CD252; Glycoprotein Gp34; gp34; OX40 antigen ligand; OX40 Ligand; OX40L; OX-40L; OX4OL; TAX transcriptionally-activated glycoprotein 1; tax-transcriptionally activated glycoprotein 1 (34kD); tax-transcriptionally activated glycoprotein 1, 34kD; TNFSF4; tumor necrosis factor (ligand) superfamily, member 4; tumor necrosis factor ligand superfamily member 4; TXGP1 TNFSF4 CD134L, CD252, GP34, OX-40L, OX4OL, TNLG2B, TXGP1 TNF superfamily member 4 tumor necrosis factor ligand superfamily member 4|CD134 ligand|OX40 antigen ligand|glycoprotein Gp34|tax-transcriptionally activated glycoprotein 1 (34kD)|tumor necrosis factor (ligand) superfamily member 4|tumor necrosis factor ligand 2B|tumor necrosis factor superfamily member 4
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on TNFSF4, check out the TNFSF4 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for TNFSF4: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
Specific Publications For Human TNFSF4/OX40L ELISA Kit PicoKine® (EK0857)
No publications found for EK0857
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Human TNFSF4/OX40L ELISA Kit PicoKine®
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2020-04-25
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is a must for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2020-04-25
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
A. Wu
Verified customer
Asked: 2019-12-31
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2019-12-31
Question
Q: What is the optimal O.D. value for OX40 Ligand ELISA kit? I performed your OX40 Ligand ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain OX40 Ligand even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2019-07-23
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this OX40 Ligand ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of OX40 Ligand that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2019-07-23
Question
Q: if the enzyme conjugated OX40 Ligand antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the OX40 Ligand antigen?
Verified Customer
Verified customer
Asked: 2018-07-23
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2018-07-23
Question
Q: can I use citrate plasma as samples in Human OX40 Ligand Picokine® ELISA Kit (Catalog # EK0857)?
Verified Customer
Verified customer
Asked: 2017-05-19
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of OX40 Ligand causing disruption of its protein structure. OX40 Ligand may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the OX40 Ligand ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2017-05-19
Question
Q: how are cell lysates prepared for use in Picokine® ELISA kits?
K. Roberts
Verified customer
Asked: 2014-12-22
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can have an affect on immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2014-12-22
