Human C-MET/HGFR ELISA Kit PicoKine™

Human C-MET/HGFR ELISA Kit PicoKine™ (96 Tests). Quantitate Human MET in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 5pg/ml.

Product Info Summary

SKU: EK0744
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Human C-MET/HGFR ELISA Kit PicoKine™

See all HGFR/c-MET products

SKU/Catalog Number

EK0744

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human C-MET/HGFR ELISA Kit PicoKine™ (96 Tests). Quantitate Human MET in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 5pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human C-MET/HGFR ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0744)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: E25-R307 (alpha) & S308-T932 (beta)

Sensitivity

<5 pg/ml

Assay Range

62.5 pg/ml - 4,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0744 is reactive to MET in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK0744 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0744-CAP Anti-Human MET Pre-coated 96-well strip microplate 1
EK0744-ST Human MET Standard 2 vials, 10 ng/tube
EK0744-DA Human MET Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)062.5125250500100020004000
O.D.0.0360.0810.1180.2360.3400.5521.0351.955

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human C-MET/HGFR ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)21060113362046441384
Standard deviation8.6131.2584.1610.8139.2895.49
CV (%)4.1%5.2%6.1%5.3%6.1%6.9%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of HGFR/c-MET in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 121019618119919610.355.2%
Sample 260167664463363826.84.2%
Sample 31336153415271377144388.236.1%
*number of samples for each test n=16.

Gene/Protein Information For MET (Source: Uniprot.Org, NCBI)

Uniprot ID

P08581

Gene ID

4233

Gene Name

MET

Full Name

Hepatocyte growth factor receptor

Weight

155.541kDa

Superfamily

protein kinase superfamily

Alternative Names

AUTS9; cMET; c-MET; EC 2.7.10; EC 2.7.10.1; hepatocyte growth factor receptor; HGF R; HGF receptor; HGF/SF receptor; HGFR; Met (c-Met); met proto-oncogene (hepatocyte growth factor receptor); met proto-oncogene tyrosine kinase; MET; oncogene MET; Proto-oncogene c-Met; RCCP2; Scatter factor receptor; SF receptor; Tyrosine-protein kinase Met MET AUTS9, DFNB97, HGFR, RCCP2, c-Met MET proto-oncogene, receptor tyrosine kinase hepatocyte growth factor receptor|HGF receptor|HGF/SF receptor|SF receptor|proto-oncogene c-Met|scatter factor receptor|tyrosine-protein kinase Met

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on MET, check out the MET Infographic

MET infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for MET: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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5 Customer Q&As for Human C-MET/HGFR ELISA Kit PicoKine™

Question

Q: can I use citrate plasma as samples in Human HGFR Picokine® ELISA Kit (Catalog # EK0744)?

Verified Customer

Verified customer

Asked: 2020-05-12

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of HGFR causing disruption of its protein structure. HGFR may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the HGFR ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2020-05-12

Question

Q: how to proceed with the analysis of ELISA data? I have obtained HGFR level in plasma.

Verified Customer

Verified customer

Asked: 2019-11-01

Answer

A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2019-11-01

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification HGFR using the EK0744 Human HGFR Picokine® ELISA Kit?

S. Zhou

Verified customer

Asked: 2019-02-28

Answer

A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples to prevent protein degradation.

Boster Scientific Support

Answered: 2019-02-28

Question

Q: how to thaw whole blood sample for HGFR ELISA after freezing?

R. Patel

Verified customer

Asked: 2019-02-15

Answer

A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test HGFR for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.

Boster Scientific Support

Answered: 2019-02-15

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2018-12-21

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2018-12-21

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