Human CA1/Carbonic anhydrase 1 ELISA Kit PicoKine™

Human CA1 ELISA Kit PicoKine™ (96 Tests). Quantitate Human CA1 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 50pg/ml.

Product Info Summary

SKU: EK0680
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Product Name

Human CA1/Carbonic anhydrase 1 ELISA Kit PicoKine™

See all Carbonic Anhydrase I/CA1 products

SKU/Catalog Number

EK0680

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human CA1 ELISA Kit PicoKine™ (96 Tests). Quantitate Human CA1 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 50pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human CA1/Carbonic anhydrase 1 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0680)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A2-F261

Sensitivity

<50 pg/ml

Assay Range

312 pg/ml - 20,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0680 is reactive to CA1 in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Application Guarantee

EK0680 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0680-CAP Anti-Human CA1 Pre-coated 96-well strip microplate 1
EK0680-ST Human CA1 Standard 2 vials, 20 ng/tube
EK0680-DA Human CA1 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)03126251250250050001000020000
O.D.0.0220.1690.2750.4450.7711.1731.4511.939

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect Carbonic Anhydrase I/CA1, Dilution ratio of 1:100, concentration in serum is around 600ng/ml.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human CA1/Carbonic anhydrase 1 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)4522590978658426569986
Standard deviation18.98157.99636.0931.54114.21559.22
CV (%)4.2%6.1%6.5%5.4%4.3%5.6%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Carbonic Anhydrase I/CA1 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 145250149147347918.653.8%
Sample 22590284128002781275396.573.5%
Sample 397869681968585819433493.845.2%
*number of samples for each test n=16.

Gene/Protein Information For CA1 (Source: Uniprot.Org, NCBI)

Uniprot ID

P00915

Gene ID

759

Gene Name

CA1

Full Name

Carbonic anhydrase 1

Weight

28.87kDa

Superfamily

alpha-carbonic anhydrase family

Alternative Names

CA1; CA-I; Car1; Carbonate dehydratase I; carbonic anhydrase 1; Carbonic anhydrase B; Carbonic Anhydrase I; carbonic anhydrase ICAB; carbonic dehydratase; EC 4.2.1.1 CA1 CA-I, CAB, Car1, HEL-S-11 carbonic anhydrase 1 carbonic anhydrase 1|carbonate dehydratase I|carbonic anhydrase B|carbonic anhydrase I|epididymis secretory protein Li 11|epididymis secretory sperm binding protein

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CA1, check out the CA1 Infographic

CA1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CA1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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8 Customer Q&As for Human CA1/Carbonic anhydrase 1 ELISA Kit PicoKine™

Question

Q: how can I thaw whole blood sample for CA1 ELISA after freezing?

Verified Customer

Verified customer

Asked: 2020-10-26

Answer

A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test CA1 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.

Boster Scientific Support

Answered: 2020-10-26

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2019-11-27

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2019-11-27

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

S. Parker

Verified customer

Asked: 2019-09-16

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2019-09-16

Question

Q: how do I analyze ELISA data? I measured CA1 level in serum.

Verified Customer

Verified customer

Asked: 2019-07-05

Answer

A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2019-07-05

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification CA1 using the EK0680 Human CA1 Picokine® ELISA Kit?

T. Smith

Verified customer

Asked: 2019-04-17

Answer

A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples to prevent protein degradation.

Boster Scientific Support

Answered: 2019-04-17

Question

Q: is it okay to use heparin plasma as samples in Human CA1 Picokine® ELISA Kit (Catalog # EK0680)?

Verified Customer

Verified customer

Asked: 2019-02-10

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of CA1 causing degradation of its protein structure. CA1 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the CA1 ELISA, treating samples with various anticoagulants and decided that heparin, EDTA or citrate can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2019-02-10

Question

Q: we need your recommendation regarding the dilution ratio of serum samples for detection of CA1 in Human serum? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.

Verified Customer

Verified customer

Asked: 2019-01-03

Answer

A: without knowing the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the CA1 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2019-01-03

Question

Q: What is the optimal O.D. value for CA1 ELISA kit? I used your CA1 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain CA1 even though the O.D. values are not very high?

Verified Customer

Verified customer

Asked: 2018-09-09

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the above example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this CA1 ELISA kit to have sensitivity of 50pg/ml, that means the minimum amount of CA1 that can be declared/interpreted as positive by the above standard is 50pg/ml.

Boster Scientific Support

Answered: 2018-09-09

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