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- Table of Contents
Facts about Carbonic anhydrase 1.
.
Human | |
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Gene Name: | CA1 |
Uniprot: | P00915 |
Entrez: | 759 |
Belongs to: |
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alpha-carbonic anhydrase family |
CA1; CA-I; Car1; Carbonate dehydratase I; carbonic anhydrase 1; Carbonic anhydrase B; Carbonic Anhydrase I; carbonic anhydrase ICAB; carbonic dehydratase; EC 4.2.1.1
Mass (kDA):
28.87 kDA
Human | |
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Location: | 8q21.2 |
Sequence: | 8; NC_000008.11 (85327608..85378113, complement) |
Cytoplasm.
If you are using the CA1 marker as part of your research, you should know that there are many different techniques to measure the protein. There are different ELISA kits, primary-secondary-ABC systems, and Super Vision detection kits. Read on to find out how to use all of them efficiently. In addition, these guides will provide you with the information you need to optimize your experiments.
The primary-secondary-ABC system enables researchers to identify proteins of interest with high sensitivity and specificity. This unique technique uses avidins conjugated to a signal molecule, typically Horseradish Peroxide (HRP), to identify specific proteins. Other detection systems use organic polymers or polysaccharides to detect proteins. The Boster Bio primary-secondary-ABC system is one of many innovative detection kits available today.
SupervisionTM and Picokine(tm) enhance ELISA sensitivities to picogram levels. The ELISA kits are validated across a broad range of samples, and images of the validation procedure can be provided upon request. The kits also feature Picoband(tm) technology, which saves 30 minutes in IHC by improving background. Picoband is backed by Boster's superior technology and technical support to ensure the highest quality detection.
GFAP, or glial fibrillary acidic protein, is an intermediate filament protein expressed in astrocytes, a type of brain cell. GFAP antibodies are the most commonly used markers for astrocytes in neurological studies. GFAP is known to be involved in many CNS processes, including neurodegeneration, schizophrenia, and Wernicke's encephalopathy.
The antibody is monoclonal and G-A-5, and reacts with GFAP in human, pig, and rat tissues. It is shelf-stable at -20°C for one year, and can be stored at 4°C for a month or six months. It contains mouse ascites fluid, BSA, and 0.01mg NaN3.
The anti-GFAP autoantibody was detected in acute plasma samples of subjects with or without a history of previous TBI. Although the result contradicts the previous study, the differences in the two groups could be explained by the small sample size and high prevalence of unreported TBI among the control group. In addition, the anti-GFAP antibody is specific for a small subset of cells, such as the astrocytes and microglia.
Enzyme-linked immunosorbent assays (ELISA) are plate-based immunoassays that use specific antibodies to detect a target antigen. The enzyme is then bound to the antigen, which is measured through the presence of a measurable product. High-affinity antibodies are the key to maximizing the sensitivity of an ELISA kit. Designed to detect CA1 in human serum, Boster's ELISA kits are ideal for use in immunoassays.
To use the ELISA kits for the CA1 marker, follow the manufacturer's instructions carefully. Boster Bio uses rabbit and mouse as the model species for development. In their research, Church D.M. and colleagues have described the development of lineage-specific biology in mouse genomes. They also provide custom services and BeNeLux deliveries to their customers. While Boster Bio's kits are recommended for non-clinical research, Boster scientists can share their results with Boster to obtain product credits and share their findings with other scientists.
The ELISA kits offered by Boster Bio can detect various biomarkers and hormones, as well as small molecules, such as 8-OHdG (DNA damage) or HSP70. They contain sufficient reagents to conduct up to 96 tests. In addition to hormone detection, Boster Bio's kits are designed to detect IL-6, IL-8, IL-10, EGF, and other cytokines.
An ELISA is a plate-based experiment designed to detect proteins and hormones in their native states. In an ELISA assay, an enzyme-linked to antibodies measures the amount of the target protein, and the reagents used in the test are then dissolved in a solution. Various formats of ELISA exist, and the principle of interaction between an antigen and an antibody differs between them. For example, Boster Bio's human GH ELISA kit utilizes a high-affinity mouse monoclonal antibody, which can detect GH in its native form. Boster Bio's ELISA kits have been validated by the company's QC department against relevant superfamilies of proteins and other similar immunogenic proteins.
Unlike ELISA kits for other biomarkers, PicokineTM sandwich ELISAs guarantee high sensitivity, specificity, and stability. These sandwich ELISA kits are supplied in 96-well format, enabling researchers to perform multiple assays using the same reagents. Moreover, Boster Bio's ELISA kits for detection of hormones can be used for a variety of applications, ranging from research to clinical practice.
The Boster Bio company was established in 1993 and has consistently specialized in the manufacture of high-sensitivity antibodies and ELISA kits. Its products have received over 28,000 citations and are used in a variety of research applications. For those in the market for small molecules ELISA kits, this company offers several services to help researchers make the best possible choice. Boster offers free technical resources and 24 hour support to its customers.
ELISA kits contain the necessary reagents for a single test. The ELISA technique uses enzyme-linked immunosorbent assays to detect specific molecules and their antigens. The results are quantified by using antibodies attached to enzymes. There are different formats of ELISA, but their underlying principle is the same: binding of the target antigen to specific antibodies. The Boster Bio ELISA kits contain high-affinity antibodies that detect native-form proteins. Boster's QC department validates every kit against relevant immunogenic proteins and superfamilies.
The Boster Bio ELISA kits can detect a variety of specific compounds, including hormones, inflammatory cytokines, oxidative stress, and DNA damage. Their reagents are standardized for use with various samples, including blood, urine, and plasma. The Boster kits also come with sufficient reagents for 96 tests. The kits are validated for a variety of samples, including human blood, so that they can detect hormones and small molecules.
The ELISA kits available from Boster Bio measure various cellular factors such as oxidative stress, inflammation, and stress hormones. Boster offers many kits and reagents, and their products have been validated across a variety of samples. To make the most out of their ELISA kits, Boster offers a variety of customizable solutions for a variety of research needs.
ELISA assays are based on the principle of enzyme-linked immunosorbent assay, a plate-based assay for the detection of antibodies, proteins, or hormones. To detect specific proteins, an antigen must be immobilized on a solid surface and complexed with a corresponding antibody. Once the two are incubated, an enzyme is created, which produces a measurable product. A high-affinity antibody coating is the key to this assay's high sensitivity.
A sandwich assay uses two monoclonal antibodies (mAbs) with different epitopes that recognize the same protein. One pulls the protein out of the solution, while the other provides a signal that indicates the presence of the target. Each kit comes with a known concentration of the recombinant protein to serve as a standard curve. In addition to standard curves, Boster Bio also offers rat ELISA kits and a rabbit lgG peroxidase-biotin complex.
PMID: 3104879 by Barlow J.H., et al. Human carbonic anhydrase I cDNA.
PMID: 2121614 by Lowe N., et al. Structure and methylation patterns of the gene encoding human carbonic anhydrase I.