Overview
| Product Name |
Human Cathepsin E ELISA Kit PicoKine™
See all Cathepsin E primary antibodies, ELISA kits and proteins |
| SKU/Catalog Number |
EK1306 |
| Storage & Handling |
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.) |
| Description |
Detect Human Cathepsin E/CTSE with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Cathepsin E/CTSE tissue specificity: Expressed abundantly in the stomach, the Clara cells of the lung and activated B-lymphocytes, and at lower levels in lymph nodes, skin and spleen. Not expressed in resting B- lymphocytes. . |
| Cite This Product |
Human Cathepsin E ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1306)
|
Product Specs
| Reactivity/Species |
Human |
| Applications |
ELISA
*Our Boster Guarantee covers the use of this product in the above
tested applications.
**For positive and negative control design, consult "Tissue specificity" under
"Protein Target Info" tab. |
| Sample Type |
cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). |
| Sensitivity |
<10pg/ml |
| Assay Range |
15.6pg/ml-1000pg/ml |
| Immunogen/Standard |
Expression system for standard: NSO; Immunogen sequence: Q18-P396 |
| Antibody Clonalities |
Capture antibody|Detection antibody
monoclonal antibody from mouse|polyclonal antibody from goat |
| Cross Reactivity |
There is no detectable cross-reactivity with other relevant proteins. |
| Pack Size |
96wells/kit, with removable strips. |
Gene/Protein Basic Information For CTSE (Source: Uniprot.org, NCBI)
| Uniprot Id | P14091 |
|---|
| NCBI Gene Id | 1510 |
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| Species Of This Entry | Human |
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| Gene Name | CTSE |
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| Protein Name | Cathepsin E |
|---|
| Superfamily | peptidase A1 family |
|---|
| Alternative Names | Cathepsin E|CATE; Cathepsin E; CTSE; EC 3.4.23; EC 3.4.23.34; erythrocyte membrane aspartic proteinase; slow-moving proteinase |
|---|
| Molecular Weight | 42794 |
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| Post Tranlational Modifications | Glycosylation; Cleavage |
|---|
| Subcellular Localization | Endosome. The proenzyme is localized to the endoplasmic reticulum and Golgi apparatus, while the mature enzyme is localized to the endosome. |
|---|
| Gene Location | 1q32.1, on Chromasome 1, gene sequence: NC_000001.11 (206009264..206023899, complement) |
|---|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For CTSE (Source: Uniprot.org, NCBI)
| Protein Function | May have a role in immune function. Probably involved in the processing of antigenic peptides during MHC class II-mediated antigen presentation. May play a role in activation-induced lymphocyte depletion in the thymus, and in neuronal degeneration and glial cell activation in the brain. |
|---|
| Tissue Specificity | Expressed abundantly in the stomach, the Clara cells of the lung and activated B-lymphocytes, and at lower levels in lymph nodes, skin and spleen. Not expressed in resting B- lymphocytes. |
|---|
| Research Areas | Adaptive Immunity; Immunology; Innate Immunity; Lipid and Metabolism |
|---|
| Related Diseases | Malignant Neoplasms; Neoplasms; Adenocarcinoma; Carcinoma; Adenoma; Pancreatic Neoplasm; Neoplasm Invasiveness; Malignant Neoplasm Of Urinary Bladder; Gastric Adenocarcinoma; Pancreatic Ductal Adenocarcinoma; Pancreatitis, Chronic; Colorectal Neoplasms; Ductal Breast Carcinoma; Pancreatitis; Colorectal Cancer; Bladder Neoplasm; Hyperplasia; Neoplasm Metastasis; Polyps |
|---|
| Related Pathways | Localization; Sensitization; Cell Proliferation; Induction Of Apoptosis; Cell Death; Innate Immune Response; Immune Response; Glycosylation; Angiogenesis; Keratinocyte Differentiation; Pathogenesis; Secretion |
|---|
| Background | Cathepsin E is a protein that in humans is encoded by the CTSE gene. It is mapped to 1q32.1. The protein encoded by this gene is a gastric aspartyl protease that functions as a disulfide-linked homodimer. This protease, which is a member of the peptidase C1 family, has a specificity similar to that of pepsin A and cathepsin D. It is an intracellular proteinase that does not appear to be involved in the digestion of dietary protein and is found in highest concentration in the surface of epithelial mucus-producing cells of the stomach. Cathepsin E is the first aspartic proteinase expressed in the fetal stomach and is found in more than half of gastric cancers. It appears, therefore, to be an oncofetal antigen, and it also has an important role in immune responses. |
|---|
| Scientific References | PMID: 2674141 by Azuma T., et al. Human gastric cathepsin E. Predicted sequence, localization to chromosome 1, and sequence homology with other aspartic proteinases.
PMID: 1370478 by Azuma T., et al. Human gastric cathepsin E gene. Multiple transcripts result from alternative polyadenylation of the primary transcripts of a single gene locus at 1q31-q32. |
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Kit Components And Assay QC Data Details Of Human Cathepsin E ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
| Catalog number | Description | Quantity |
| EK1306-CAP | 96-well plate precoated with anti-Human Cathepsin E antibody | 1 |
| EK1306-ST | lyophilized recombinant Human Cathepsin E standard | 10ng/tube |
| EK1306-DA | biotinylated anti-Human Cathepsin E antibody | 130ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 130ul |
| AR1106-1 | sample diluent buffer | 30ml |
| AR1106-2 | antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EK1306 Human Cathepsin E ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
| Concentration(pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.019 | 0.103 | 0.165 | 0.280 | 0.501 | 0.908 | 1.472 | 2.188 |
We measured random samples of Human Cathepsin E ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision |
|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 27 | 63 | 416 | 30 | 82 | 435 |
| Standard deviation | 1.30 | 3.59 | 19.14 | 1.53 | 4.84 | 27.84 |
| CV(%) | 4.8% | 5.7% | 4.6% | 5.1% | 5.9% | 6.4% |
We measured the performance consistency of Human Cathepsin E ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|
| Sample 1 | 27 | 24 | 25 | 26 | 25 | 1.11 | 4.4% |
| Sample 2 | 63 | 61 | 69 | 71 | 66 | 4.12 | 6.2% |
| Sample 3 | 416 | 414 | 399 | 412 | 410 | 6.64 | 1.6% |
*number of samples for each test n=16.
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
See all Cathepsin E primary antibodies,
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