Overview
| Product Name |
Human Cathepsin E ELISA Kit PicoKine™
See more Cathepsin E products |
| Catalog# |
EK1306 |
| Storage & Handling |
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles. |
| Description |
Human Cathepsin E ELISA Kit PicoKine™ (96 Tests). Quantitate Human CTSE in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).. Sensitivity: 10pg/ml. |
| Cite This Product |
Human Cathepsin E ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1306)
|
| ELISA Validation |
ELISA Validation Information
|
Product Specs
| Reactivity/Species |
Human |
| Applications |
ELISA
*Our Boster Guarantee covers the use of this product in the above
tested applications.
**For positive and negative control design, consult "Tissue specificity" under
"Protein Target Info" tab. |
| Sample Type |
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
| Sensitivity |
<10 pg/ml |
| Assay Range |
15.6 pg/ml - 1,000 pg/ml |
| Immunogen/Standard |
Expression system for standard: NS0; Immunogen sequence: Q18-P396 |
| Antibody Clonalities |
Capture antibody|Detection antibody
monoclonal antibody from mouse|polyclonal antibody from goat |
| Cross Reactivity |
There is no detectable cross-reactivity with other relevant proteins. |
| Pack Size |
96 wells/kit, with removable strips. |
Gene/Protein Basic Information For CTSE (Source: Uniprot.org, NCBI)
| Uniprot Id | P14091 |
|---|
| NCBI Gene Id | 1510 |
|---|
| Species Of This Entry | Human |
|---|
| Gene Name | CTSE |
|---|
| Protein Name | Cathepsin E |
|---|
| Superfamily | peptidase A1 family |
|---|
| Alternative Names | Cathepsin E|CATE; Cathepsin E; CTSE; EC 3.4.23; EC 3.4.23.34; erythrocyte membrane aspartic proteinase; slow-moving proteinase |
|---|
| Molecular Weight | 42794 |
|---|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on CTSE, check out the CTSE Infographic
We have 30,000+ of these available, one for each gene! Check them out.
Want a nice infographic on your next protein? Boster's got them all. All proteins in the human genome, and some, can be found in the Boster Bio gene infographics. Download it and share it with your colleagues and friends. Big size posters available upon request.
In this infographic you will see the following information for CTSE: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. Some times it even contains some opt-ed articles the Boster team on the scientific news and clinical impacts of this protein, though not all have that. Too many proteins, you know.
Take me to the CTSE infographic
Kit Components And Assay QC Data Details Of Human Cathepsin E ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
| Catalog number | Description | Quantity |
| EK1306-CAP |
Anti-Human CTSE Pre-coated 96-well strip microplate | 1 |
| EK1306-ST | Human CTSE Standard | 2 vials, 10 ng/tube |
| EK1306-DA | Human CTSE Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
This data is generated from a recent batch of EK1306 Human Cathepsin E ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.019 | 0.103 | 0.165 | 0.280 | 0.501 | 0.908 | 1.472 | 2.188 |
We measured random samples of Human Cathepsin E ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision |
|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 27 | 63 | 416 | 30 | 82 | 435 |
| Standard deviation | 1.30 | 3.59 | 19.14 | 1.53 | 4.84 | 27.84 |
| CV (%) | 4.8% | 5.7% | 4.6% | 5.1% | 5.9% | 6.4% |
We measured the performance consistency of Human Cathepsin E ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|
| Sample 1 | 27 | 24 | 25 | 26 | 25 | 1.11 | 4.4% |
| Sample 2 | 63 | 61 | 69 | 71 | 66 | 4.12 | 6.2% |
| Sample 3 | 416 | 414 | 399 | 412 | 410 | 6.64 | 1.6% |
*number of samples for each test n=16.
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
Other Recommended Resources
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Total number of citations: 0
Contact us with any questions at [email protected] or go to contact us
Questions and answers from customers related to EK1306 Human Cathepsin E ELISA Kit PicoKine™
7 Related Questions
Question
Q: can I use citrate plasma as samples in Human Cathepsin E Picokine® ELISA Kit (Catalog # EK1306)?
Verified Customer
Asked: 2020-10-22
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of Cathepsin E causing disruption of its protein structure. Cathepsin E may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the Cathepsin E ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2020-10-22
Question
Q: What is the optimal O.D. value for Cathepsin E ELISA kit? I used your Cathepsin E ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain Cathepsin E even though the O.D. values are not very high?
V. Brown
Asked: 2020-07-05
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. in your example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Cathepsin E ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of Cathepsin E that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2020-07-05
Question
Q: how do I analyze ELISA data? I have obtained Cathepsin E level in plasma.
Verified Customer
Asked: 2019-07-02
Boster Scientific Support
Answered: 2019-07-02
Question
Q: how can I thaw whole blood sample for Cathepsin E ELISA after freezing?
Verified Customer
Asked: 2018-12-13
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Cathepsin E for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2018-12-13
Question
Q: if the enzyme conjugated Cathepsin E antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Cathepsin E antigen?
Verified Customer
Asked: 2018-11-15
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2018-11-15
Question
Q: Can Cathepsin E ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Asked: 2018-09-28
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2018-09-28
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement Cathepsin E using the EK1306 Human Cathepsin E Picokine® ELISA Kit?
Verified Customer
Asked: 2014-07-03
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to avoid protein degradation.
Boster Scientific Support
Answered: 2014-07-03
