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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum and plasma (heparin, EDTA).|
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Human CREG1 ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human CREG1 ELISA Kit PicoKine™ (96 Tests). Quantitate Human CREG1 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human CREG1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1956)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: E.coli; Immunogen sequence: R32-Q220
312 pg/ml - 20,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK1956 is reactive to CREG1 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
EK1956 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of CREG
This gene is mapped to 1q24.2. The adenovirus E1A protein both activates and represses gene expression to promote cellular proliferation and inhibit differentiation. The protein encoded by this gene antagonizes transcriptional activation and cellular transformation by E1A. This protein shares limited sequence similarity with E1A and binds both the general transcription factor TBP and the tumor suppressor pRb in vitro. This gene may contribute to the transcriptional control of cell growth and differentiation.
|EK1956-CAP||Anti-Human CREG1 Pre-coated 96-well strip microplate||1|
|EK1956-ST||Human CREG1 Standard||2 vials, 20 ng/tube|
|EK1956-DA||Human CREG1 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human CREG1 PicoKine ELISA Kitstandard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect CREG, Dilution ratio of 1:1, concentration in serum is around 6ng/ml.
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human CREG1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of CREG in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For CREG1 (Source: Uniprot.Org, NCBI)
cellular repressor of E1A-stimulated genes 1CREG; cellular repressor of E1A-stimulated genes; CREG; CREG1; protein CREG1 CREG1 CREG cellular repressor of E1A stimulated genes 1 protein CREG1*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on CREG1, check out the CREG1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CREG1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
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