Human soluble EGFR ELISA Kit PicoKine™

Human soluble EGFR ELISA Kit PicoKine™ (96 Tests). Quantitate Human EGFR in cell culture supernatants, serum, plasma (heparin, EDTA) and human milk. Sensitivity: 1pg/ml. Cited in 5 publication(s).

Product Info Summary

SKU: EK0327
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum, plasma (heparin, EDTA) and human milk.

Product Name

Human soluble EGFR ELISA Kit PicoKine™

See all EGFR products

SKU/Catalog Number

EK0327

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human soluble EGFR ELISA Kit PicoKine™ (96 Tests). Quantitate Human EGFR in cell culture supernatants, serum, plasma (heparin, EDTA) and human milk. Sensitivity: 1pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human soluble EGFR ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0327)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: M1-S645

Sensitivity

<1 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0327 is reactive to EGFR in Human samples

Validated Sample Types

cell culture supernatants, serum, plasma (heparin, EDTA) and human milk.

Application Guarantee

EK0327 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0327-CAP Anti-Human EGFR Pre-coated 96-well strip microplate 1
EK0327-ST Human EGFR Standard 2 vials, 10 ng/tube
EK0327-DA Human EGFR Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631262512500025000500010000
O.D.0.0370.1050.1550.2540.4630.8371.3051.773

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human soluble EGFR ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)2891634552631317315572
Standard deviation13.5881.7408.9218.78102.12417.9
CV (%)4.7%5%7.4%6%5.9%7.5%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of EGFR in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 128931628527929214.164.8%
Sample 21634169017371686168636.462.1%
Sample 355265048507050565175202.83.9%
*number of samples for each test n=16.

Gene/Protein Information For EGFR (Source: Uniprot.Org, NCBI)

Uniprot ID

P00533

Gene ID

1956

Gene Name

EGFR

Full Name

Epidermal growth factor receptor

Weight

134.277kDa

Superfamily

protein kinase superfamily

Alternative Names

avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; EC 2.7.10; EC 2.7.10.1; EGF R; EGFR; epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b)oncogene homolog); epidermal growth factor receptor; ErbB; ErbB1; ERBB1PIG61; HER1; HER-1; mENA; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1 EGFR ERBB, ERBB1, ERRP, HER1, NISBD2, PIG61, mENA epidermal growth factor receptor epidermal growth factor receptor|avian erythroblastic leukemia viral (v-erb-b) oncogene homolog|cell growth inhibiting protein 40|cell proliferation-inducing protein 61|epidermal growth factor receptor tyrosine kinase domain|erb-b2 receptor tyrosine kinase 1|proto-oncogene c-ErbB-1|receptor tyrosine-protein kinase erbB-1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on EGFR, check out the EGFR Infographic

EGFR infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for EGFR: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0327 has been cited in 5 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Epidermal growth factor receptor and podocin predict nephropathy progression in type 2 diabetic patients through interaction with the autophagy influencer ULK-1

Qin Li L, Huang Hl, Ping Jl, Xu W, Li J, Dai Lc. Apmis. 2011 Feb;119(2):103-10. Doi: 10.1111/J.1600-0463.2010.02699.X. Epub 2010 Dec 1. Expression Of Midkine And Endoglin In Breast Carcinomas With Different Immunohistochemical Profiles.

Zhang H, Mao Y, Zhang F, Ye C, Tong H, Su Y, Zhu J. Mol Cell Biochem. 2014 Jun;391(1-2):77-84. Doi: 10.1007/S11010-014-1989-3. Epub 2014 Feb 25. The Inhibitory Effect Of A New Scfv/Tp Protein As Sirna Delivery System To Target Hwapl In Cervical Ca...

Achmad A, Hanaoka H, Yoshioka H, Yamamoto S, Tominaga H, Araki T, Ohshima Y, Oriuchi N, Endo K. Cancer Sci. 2012 Mar;103(3):600-5. Doi: 10.1111/J.1349-7006.2011.02166.X. Epub 2011 Dec 23. Predicting Cetuximab Accumulation In Kras Wild-Type And Kra...

Ge C, Yu M, Petitte Jn, Zhang C. Biol Reprod. 2009 Mar;80(3):528-36. Doi: 10.1095/Biolreprod.108.072728. Epub 2008 Nov 12. Epidermal Growth Factor-Induced Proliferation Of Chicken Primordial Germ Cells: Involvement Of Calcium/Protein Kinase C And ...

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8 Customer Q&As for Human soluble EGFR ELISA Kit PicoKine™

Question

Q: can I use citrate plasma as samples in Human EGFR Picokine® ELISA Kit (Catalog # EK0327)?

Verified Customer

Verified customer

Asked: 2020-08-13

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of EGFR causing disruption of its protein structure. EGFR may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the EGFR ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2020-08-13

Question

Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?

Y. Jones

Verified customer

Asked: 2020-07-15

Answer

A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer may affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2020-07-15

Question

Q: Can EGFR ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

L. Jha

Verified customer

Asked: 2020-07-01

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2020-07-01

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2019-11-29

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2019-11-29

Question

Do you have a non-cross reactivity validation data for the EK0327 kit with mouse EGFR? Does it react with mouse EGFR?

Verified customer

Asked: 2019-10-10

Answer

Our lab did not test the Human soluble EGFR ELISA Kit PicoKine EK0327 for the cross reactivity with mouse EGFR. Currently, we do not have the specific recombinant mouse EGFR protein for testing.

Boster Scientific Support

Answered: 2019-10-11

Question

Q: how can I thaw whole blood sample for EGFR ELISA after freezing?

Verified Customer

Verified customer

Asked: 2019-06-29

Answer

A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test EGFR for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2019-06-29

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

B. Evans

Verified customer

Asked: 2018-04-05

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2018-04-05

Question

Q: if the enzyme conjugated EGFR antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the EGFR antigen?

Verified Customer

Verified customer

Asked: 2017-03-17

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2017-03-17

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