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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum, plasma (heparin, EDTA) and human milk.|
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Human soluble EGFR ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human soluble EGFR ELISA Kit PicoKine™ (96 Tests). Quantitate Human EGFR in cell culture supernatants, serum, plasma (heparin, EDTA) and human milk. Sensitivity: 1pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human soluble EGFR ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0327)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: M1-S645
156 pg/ml - 10,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK0327 is reactive to EGFR in Human samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin, EDTA) and human milk.
EK0327 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of EGFR
The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands.1 It is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). EGFR exists on the cell surface and is activated by binding of its specific ligands, including epidermal growth factor and transforming growth factor alpha (TGFalpha). EGFR and its ligands are cell signaling molecules involved in diverse cellular functions, including cell proliferation, differentiation, motility, and survival, and in tissue development. Mutations that lead to EGFR overexpression (known as upregulation) or overactivity have been associated with a number of cancers, including lung cancer and glioblastoma multiforme. In this latter case a more or less specific mutation of EGFR, called EGFRvIII is often observed.
|EK0327-CAP||Anti-Human EGFR Pre-coated 96-well strip microplate||1|
|EK0327-ST||Human EGFR Standard||2 vials, 10 ng/tube|
|EK0327-DA||Human EGFR Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human EGFR PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human soluble EGFR ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of EGFR in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For EGFR (Source: Uniprot.Org, NCBI)
Epidermal growth factor receptor
protein kinase superfamily
avian erythroblastic leukemia viral (v-erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; EC 2.7.10; EC 22.214.171.124; EGF R; EGFR; epidermal growth factor receptor (avian erythroblastic leukemia viral (v-erb-b)oncogene homolog); epidermal growth factor receptor; ErbB; ErbB1; ERBB1PIG61; HER1; HER-1; mENA; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1 EGFR ERBB, ERBB1, ERRP, HER1, NISBD2, PIG61, mENA epidermal growth factor receptor epidermal growth factor receptor|avian erythroblastic leukemia viral (v-erb-b) oncogene homolog|cell growth inhibiting protein 40|cell proliferation-inducing protein 61|epidermal growth factor receptor tyrosine kinase domain|erb-b2 receptor tyrosine kinase 1|proto-oncogene c-ErbB-1|receptor tyrosine-protein kinase erbB-1*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on EGFR, check out the EGFR Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for EGFR: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
EK0327 has been cited in 5 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Epidermal growth factor receptor and podocin predict nephropathy progression in type 2 diabetic patients through interaction with the autophagy influencer ULK-1
Qin Li L, Huang Hl, Ping Jl, Xu W, Li J, Dai Lc. Apmis. 2011 Feb;119(2):103-10. Doi: 10.1111/J.1600-0463.2010.02699.X. Epub 2010 Dec 1. Expression Of Midkine And Endoglin In Breast Carcinomas With Different Immunohistochemical Profiles.
Zhang H, Mao Y, Zhang F, Ye C, Tong H, Su Y, Zhu J. Mol Cell Biochem. 2014 Jun;391(1-2):77-84. Doi: 10.1007/S11010-014-1989-3. Epub 2014 Feb 25. The Inhibitory Effect Of A New Scfv/Tp Protein As Sirna Delivery System To Target Hwapl In Cervical Ca...
Achmad A, Hanaoka H, Yoshioka H, Yamamoto S, Tominaga H, Araki T, Ohshima Y, Oriuchi N, Endo K. Cancer Sci. 2012 Mar;103(3):600-5. Doi: 10.1111/J.1349-7006.2011.02166.X. Epub 2011 Dec 23. Predicting Cetuximab Accumulation In Kras Wild-Type And Kra...
Ge C, Yu M, Petitte Jn, Zhang C. Biol Reprod. 2009 Mar;80(3):528-36. Doi: 10.1095/Biolreprod.108.072728. Epub 2008 Nov 12. Epidermal Growth Factor-Induced Proliferation Of Chicken Primordial Germ Cells: Involvement Of Calcium/Protein Kinase C And ...
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