Human ErbB-2 ELISA Kit PicoKine™
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human ErbB-2. 96wells/kit, with removable strips. Cited in 2 publication(s).
Human ErbB-2 ELISA Kit PicoKine™ Info At A Glance
|Size:||96wells/kit, with removable strips.|
|Sample Type:||cell culture supernates, cell lysates, serum and plasma(heparin, EDTA)|
|Sample Volume:||100μl per well|
|Product Name||Human ErbB-2 ELISA Kit PicoKine™
See all ErbB2/Her2 primary antibodies, ELISA kits and proteins
|Storage & Handling||Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)|
|Description||Detect Human ERBB2 with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, serum and plasma(heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. ERBB2 tissue specificity: Expressed in a variety of tumor tissues including primary breast tumors and tumors from small bowel, esophagus, kidney and mouth. .|
|Cite This Product||Human ErbB-2 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0756)|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab.
|Sample Type||cell culture supernates, cell lysates, serum and plasma(heparin, EDTA)|
|Immunogen/Standard||Expression system for standard: NSO; Immunogen sequence: T23-T652|
|Antibody Clonalities||Capture antibody|Detection antibody
polyclonal antibody from goat|polyclonal antibody from goat
|Cross Reactivity||There is no detectable cross-reactivity with other relevant proteins.|
|Pack Size||96wells/kit, with removable strips.|
Gene/Protein Basic Information For ERBB2 (Source: Uniprot.org, NCBI)
|NCBI Gene Id||2064|
|Species Of This Entry||Human|
|Protein Name||Receptor tyrosine-protein kinase erbB-2|
|Superfamily||protein kinase superfamily|
|Alternative Names||ErbB2/Her2|CD340 antigen; CD340; c-erb B2/neu protein; EC 2.7.10; EGFR2; ErbB2; HER2; HER-2; HER2EC 188.8.131.52; herstatin; Metastatic lymph node gene 19 protein; MLN 19; MLN19; Neu Oncogene; NEUHER-2/neu; neuroblastoma/glioblastoma derived oncogene homolog; NGL; NGLTKR1; p185erbB2; Proto-oncogene c-ErbB-2; Proto-oncogene Neu; receptor tyrosine-protein kinase erbB-2; TKR1; Tyrosine kinase-type cell surface receptor HER2; v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 2(neuro/glioblastoma derived oncogene homolog); v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastomade|
|Post Tranlational Modifications||Phosphorylation; Cleavage; Methylation; Ubiquitination; Dephosphorylation; Biotinylation; Acetylation; Glycosylation; Reduction; Demethylation; Prenylation; Deacetylation; Sumoylation; Myristoylation; Phosphorothioation; Ribosylation; Farnesylation; Oxidation; Carboxylation; Hydroxylation|
|Subcellular Localization||[Isoform 1]: Cell membrane; Single-pass type I membrane protein. Early endosome. Cytoplasm, perinuclear region. Nucleus. Translocation to the nucleus requires endocytosis, probably endosomal sorting and is mediated by importin beta-1/KPNB1. Also detected in VPS35-positive endosome-to-TGN retrograde vesicles (PubMed:31138794).; [Isoform 2]: Cytoplasm. Nucleus.; [Isoform 3]: Cytoplasm. Nucleus.|
|Gene Location||17q12, on Chromasome 17, gene sequence: NC_000017.11 (39688084..39728662)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For ERBB2 (Source: Uniprot.org, NCBI)
|Protein Function||Protein tyrosine kinase that is part of several cell surface receptor complexes, but that apparently needs a coreceptor for ligand binding. Essential component of a neuregulin-receptor complex, although neuregulins do not interact with it alone. GP30 is a potential ligand for this receptor. Regulates outgrowth and stabilization of peripheral microtubules (MTs). Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane. This prevents the phosphorylation of APC and CLASP2, allowing its association with the cell membrane. In turn, membrane-bound APC allows the localization of MACF1 to the cell membrane, which is required for microtubule capture and stabilization. .|
|Tissue Specificity||Expressed in a variety of tumor tissues including primary breast tumors and tumors from small bowel, esophagus, kidney and mouth.|
|Research Areas||Breast Cancer; Cancer; Cellular Markers; Core ESC-Like Genes; Phospho-Specific; Protein Kinase; Stem Cell Markers; Tumor Suppressors|
|Related Diseases||Malignant Neoplasms; Neoplasms; Mammary Neoplasms; Malignant Neoplasm Of Breast; Carcinoma; Neoplasm Metastasis; Cell Transformation, Neoplastic; Adenocarcinoma; Malignant Paraganglionic Neoplasm; Ductal Breast Carcinoma; Cell Invasion; Neoplasm Invasiveness; Carcinoma Breast Stage Iv; Lung Neoplasms; Lymphatic Metastasis; Malignant Neoplasm Of Lung; Carcinogenesis; Ductal Carcinoma; Stomach Neoplasms|
|Related Pathways||Cell Proliferation; Cell Growth; Cell Cycle; Localization; Angiogenesis; Pathogenesis; Cell Death; Cell Migration; Secretion; Oncogenesis; Cell Motility; Drug Resistance; Endocytosis; Immune Response; Methylation; Translation; Cell Adhesion; Rna Interference; Induction Of Apoptosis; Cell Cycle Arrest|
|Background||HER2/neu(also known as ErbB-2) stands for "Human Epidermal growth factor Receptor 2" and is a protein giving higher aggressiveness in breast cancers. It is a member of the ErbB protein family, more commonly known as the epidermal growth factor receptor family. HER2/neu has also been designated as CD340(cluster of differentiation 340) and p185. It is encoded by the ERBB2 gene.HER2 is a cell membrane surface-bound receptor tyrosine kinase and is normally involved in the signal transduction pathways leading to cell growth and differentiation. It is encoded within the genome by HER2/neu, a known proto-oncogene. HER2 is thought to be an orphan receptor, with none of the EGF family of ligands able to activate it. However, ErbB receptors dimerise on ligand binding, and HER2 is the preferential dimerisation partner of other members of the ErbB family.1 The HER2 gene is a proto-oncogene located at the long arm of human chromosome 17(17q21-q22)2.|
|Scientific References||PMID: 3003577 by Yamamoto T., et al. Similarity of protein encoded by the human c-erb-B-2 gene to epidermal growth factor receptor.|
PMID: 2999974 by Coussens L., et al. Tyrosine kinase receptor with extensive homology to EGF receptor shares chromosomal location with neu oncogene.
Kit Components And Assay QC Data Details Of Human ErbB-2 ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
|EK0756-CAP||96-well plate precoated with anti-Human ErbB2/Her2 antibody||1|
|EK0756-ST||lyophilized recombinant Human ErbB2/Her2 standard||10ng/tube|
|EK0756-DA||biotinylated anti-Human ErbB2/Her2 antibody||130ul|
|AR1106-1||sample diluent buffer||30ml|
|AR1106-2||antibody diluent buffer||12ml|
|AR1106-3||ABC diluent buffer||12ml|
|AR1104||TMB color developing agent||10ml|
|AR1105||TMB stop solution||10ml|
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EK0756 Human ErbB-2 ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
We measured random samples of Human ErbB-2 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We measured the performance consistency of Human ErbB-2 ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
|Lots||Lot1 (pg/ml)||Lot2 (pg/ml)||Lot3 (pg/ml)||Lot4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
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Total number of citations: 2
|Tao L, Suhua C, Juanjuan C, Zongzhi Y, Juan X, Dandan Z. Virol J. 2011 Mar 11;8:114. Doi: 10.1186/1743-422X-8-114. In Vitro Study On Human Cytomegalovirus Affecting Early Pregnancy Villous Evt'S Invasion Function.||PubMed ID 21392403|
|A phase 1 study of weekly dosing of trastuzumab emtansine (T%u2010DM1) in patients with advanced human epidermal growth factor 2%u2013positive breast cancer||PubMed ID 22648179|
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