Human IGFBP7/Igfbp Rp1 ELISA Kit PicoKine™

Human IGFBP7/Igfbp Rp1 ELISA Kit PicoKine™ (96 Tests). Quantitate Human IGFBP7 in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 20pg/ml. Cited in 2 publication(s).

Product Info Summary

SKU: EK0991
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum, plasma (heparin, EDTA) and urine.

Product Name

Human IGFBP7/Igfbp Rp1 ELISA Kit PicoKine™

See all IGFBP-rp1/IGFBP-7 products

SKU/Catalog Number

EK0991

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human IGFBP7/Igfbp Rp1 ELISA Kit PicoKine™ (96 Tests). Quantitate Human IGFBP7 in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 20pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human IGFBP7/Igfbp Rp1 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0991)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: D30-L282

Sensitivity

<20 pg/ml

Assay Range

625 pg/ml - 40,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0991 is reactive to IGFBP7 in Human samples

Validated Sample Types

cell culture supernatants, serum, plasma (heparin, EDTA) and urine.

Application Guarantee

EK0991 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0991-CAP Anti-Human IGFBP7 Pre-coated 96-well strip microplate 1
EK0991-ST Human IGFBP7 Standard 2 vials, 40 ng/tube
EK0991-DA Human IGFBP7 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)0625125025005000100002000040000
O.D.0.0010.0230.0350.0700.1610.3450.8121.836

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect IGFBP-rp1/IGFBP-7, Dilution ratio of 1:10, concentration in serum is around 50ng/ml.

Some articles we found to cite concentrations of IGFBP-rp1/IGFBP-7 in samples: 24180466 (Pubmed IDs).

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human IGFBP7/Igfbp Rp1 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)16294265188821617442720605
Standard deviation114.03319.871340.62118.04358.581669
CV (%)7%7.5%7.1%7.3%8.1%8.1%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of IGFBP-rp1/IGFBP-7 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 11629149815071608156058.553.7%
Sample 242653682431237814010281.187%
Sample 318882209351988321797203741096.225.3%
*number of samples for each test n=16.

Gene/Protein Information For IGFBP7 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q16270

Gene ID

3490

Gene Name

IGFBP7

Full Name

Insulin-like growth factor-binding protein 7

Weight

29.13kDa

Alternative Names

angiomodulin; FSTL2; IBP-7; IGFBP7; IGFBP-7; IGFBP-7PGI2-stimulating factor; IGFBP-7v; IGFBPrp1; IGFBP-rp1; insulin-like growth factor binding protein 7; insulin-like growth factor-binding protein 7; MAC25 protein; Mac25; MAC25IGF-binding protein 7; Prostacyclin-stimulating factor; PSF; PSFAGM; TAF; Tumor-derived adhesion factor IGFBP7 AGM, FSTL2, IBP-7, IGFBP-7, IGFBP-7v, IGFBPRP1, MAC25, PSF, RAMSVPS, TAF insulin like growth factor binding protein 7 insulin-like growth factor-binding protein 7|IGF-binding protein 7|IGFBP-rP1|PGI2-stimulating factor|angiomodulin|prostacyclin-stimulating factor|tumor-derived adhesion factor

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on IGFBP7, check out the IGFBP7 Infographic

IGFBP7 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for IGFBP7: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0991 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Kevin Esmeijer, Abraham Schoe, L Renee Ruhaak, Ellen K Hoogeveen, Darius Soonawala, Fred P H T M Romijn, Maryam R Shirzada, Jaap T van Dissel, Christa M Cobbaert, Johan W de Fijter. 2021 Jan 13;11(1):1071. doi: 10.1038/s41598-020-80196-2. The predictive value of TIMP-2 and IGFBP7 for kidney failure and 30-day mortality after elective cardiac surgery

Tissue and serum IGFBP7 protein as biomarker in high-grade soft tissue sarcoma

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16 Customer Q&As for Human IGFBP7/Igfbp Rp1 ELISA Kit PicoKine™

Question

Q: What is the optimal O.D. value for IGFBP-rp1 ELISA kit? I used your IGFBP-rp1 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain IGFBP-rp1 even though the O.D. values are not very high?

Y. Turner

Verified customer

Asked: 2020-12-01

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. in the above example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this IGFBP-rp1 ELISA kit to have sensitivity of 20pg/ml, that means the minimum amount of IGFBP-rp1 that can be declared/interpreted as positive by the above standard is 20pg/ml.

Boster Scientific Support

Answered: 2020-12-01

Question

Q: we need your recommendation regarding the dilution ratio of serum samples for detection of IGFBP-rp1 in Human serum? I am trying to measure a few parameters and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.

P. Moore

Verified customer

Asked: 2020-11-15

Answer

A: unable to understand the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the IGFBP-rp1 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2020-11-15

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

Verified Customer

Verified customer

Asked: 2020-10-10

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2020-10-10

Question

Hi Boster Biological Technology, A. If we purchase 50 - 60 kits do you offer any price breaks? can you provide the reagents in bulk packaging? what would be the turn around time for a larger order like this? B. Is the insert on line the current insert? If not, could you please provide me with the most updated version? C. Do you sell the standard antigen separately? D. Do you sell controls for the assay?

Verified Customer

Verified customer

Asked: 2020-04-09

Answer

Please see the answers for your questions. B.The insert on line is the current insert C.We do not sell the standard antigen. D.We sell controls for the assay. The controls we usually provided are recombinant proteins. Does the customer have any requirements for the controls? We usually provide a control of medium level of the assay range of the kit. An expected range will be provided with the controls. It is lot speficic. Catalog number for the control is

Boster Scientific Support

Answered: 2020-04-09

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

B. Babu

Verified customer

Asked: 2019-12-23

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2019-12-23

Question

What is the active ingredient of the stop solution and its concentration for EK0991?

Verified customer

Asked: 2019-12-03

Answer

The active ingredient of the stop solution for the Human IGFBP7/Igfbp Rp1 ELISA Kit PicoKine™ (EK0991) is Sulfuric acid with a 0.02%concentration.

Boster Scientific Support

Answered: 2019-12-03

Question

What type of preservative is used in EK0991? What is the concentration and state (liquid/lyophilized) of the preservative?

Verified customer

Asked: 2019-12-03

Answer

All components of the Human IGFBP7/Igfbp Rp1 ELISA Kit PicoKine™ (EK0991) have Sodium azide (0.02%) except for Avidin-Biotin Peroxidase complex which has Proclin (0.02%). Only Recombinant Human IGFBP7 standard is lyophilized (0.02 mg/vial). Rest are liquid.

Boster Scientific Support

Answered: 2019-12-03

Question

If the components of EK0991 are lyophilized, what is the concentration prior to reconstitution (i.e. mass of preservative used/ total mass of lyophilized vial contents)?

Verified customer

Asked: 2019-12-03

Answer

The concentration for the Human IGFBP7/Igfbp Rp1 ELISA Kit PicoKine™ (EK0991) prior to reconstitution is still 0.02%.

Boster Scientific Support

Answered: 2019-12-03

Question

What is the expression methood used for EK0991 standards?

Verified customer

Asked: 2019-12-03

Answer

For the Human IGFBP7/Igfbp Rp1 ELISA Kit PicoKine™ (EK0991), the expression method used is NSO expression system.

Boster Scientific Support

Answered: 2019-12-03

Question

Q: is it okay to use citrate plasma as samples in Human IGFBP-rp1 Picokine® ELISA Kit (Catalog # EK0991)?

Verified Customer

Verified customer

Asked: 2019-08-22

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of IGFBP-rp1 causing degradation of its protein structure. IGFBP-rp1 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the IGFBP-rp1 ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2019-08-22

Question

Q: how do I analyze ELISA data? I measured IGFBP-rp1 level in serum.

A. Huang

Verified customer

Asked: 2019-08-08

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2019-08-08

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2018-03-29

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2018-03-29

Question

Catalog number: EK0991 Could you please help me to answer a question from one of my customers about your product? See below for further details. QUESTION?I have many samples to prepare and the provided diluent buffer is not enough. Can I use another kind of buffer to replace the provided diluent buffer in this kit? What is the recommended buffer for replacement?

Verified Customer

Verified customer

Asked: 2017-10-20

Answer

We do not recommed the customer prepare the buffer by himself. If the amount of sample diluent is not enough, the customer can dilute the sample with PBS first and then use the sample diluent buffer in the kit. PBS first and sample diluent buffer later. Or the customer can buy extra sample diluent buffer.

Boster Scientific Support

Answered: 2017-10-20

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis IGFBP-rp1 using the EK0991 Human IGFBP-rp1 Picokine® ELISA Kit?

V. Lopez

Verified customer

Asked: 2016-06-29

Answer

A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples to avoid protein degradation.

Boster Scientific Support

Answered: 2016-06-29

Question

Q: how can I thaw whole blood sample for IGFBP-rp1 ELISA after freezing?

G. Patel

Verified customer

Asked: 2015-01-19

Answer

A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test IGFBP-rp1 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2015-01-19

Question

Q: Can IGFBP-rp1 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

Verified Customer

Verified customer

Asked: 2014-10-23

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is needed. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2014-10-23

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