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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum and plasma (heparin, EDTA).|
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Human FGF2 ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human FGF2 ELISA Kit PicoKine™ (96 Tests). Quantitate Human FGF2 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human FGF2 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0342)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: E.coli; Immunogen sequence: P143-S288
62.5 pg/ml - 4,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK0342 is reactive to FGF2 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
EK0342 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of FGF basic/FGF2/bFGF
FGF2 has been implicated in a multitude of physiologic and pathologic processes, including limb development, angiogenesis, wound healing, and tumor growth. Human FGF2 shares 96% and 97% amino acid sequence homology with mouse and rat respectively. FGF2 belongs to the fibroblast growth factor (FGF) family. Fibroblast growth factors (FGFs) exhibit widespread mitogenic and neurotrophic activities. Nine members of the family are currently known, and FGF-1 and FGF-2 are present in relatively high levels in CNS. FGF-2 is expressed by at low levels in many tissues and cell types and reaches high concentrations in brain and pituitary.
|EK0342-CAP||Anti-Human FGF2 Pre-coated 96-well strip microplate||1|
|EK0342-ST||Human FGF2 Standard||2 vials, 10 ng/tube|
|EK0342-DA||Human FGF2 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human FGF2 ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect FGF basic/FGF2/bFGF, Dilution ratio of 1:1, concentration in serum is around 150pg/ml.
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human FGF2 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of FGF basic/FGF2/bFGF in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For FGF2 (Source: Uniprot.Org, NCBI)
Fibroblast growth factor 2
heparin-binding growth factors family
basic fibroblast growth factor bFGF; Basic fibroblast growth factor; bFGF; FGF basic; FGF2; FGF-2; FGFBprostatropin; fibroblast growth factor 2 (basic); HBGF-2; heparin-binding growth factor 2; Prostatropin FGF2 BFGF, FGF-2, FGFB, HBGF-2 fibroblast growth factor 2 fibroblast growth factor 2|basic fibroblast growth factor bFGF|fibroblast growth factor 2 (basic)|heparin-binding growth factor 2|prostatropin*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on FGF2, check out the FGF2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for FGF2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
EK0342 has been cited in 7 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
The impact of Aggregatibacter actinomycetemcomitans biofilm-derived effectors following antimicrobial photodynamic therapy on cytokine production in human gingival fibroblasts
In vitro and in vivo study of hydralazine, a potential anti-angiogenic agent
Self‐Adaptive All‐In‐One Delivery Chip for Rapid Skin Nerves Regeneration by Endogenous Mesenchymal Stem Cells
Overcoming Autocrine FGF Signaling-Induced Heterogeneity in Naive Human ESCs Enables Modeling of Random X Chromosome Inactivation
Wang J, Wang Z, Yao Y, Wu J, Tang X, Gu T, Li G. Tuberculosis (Edinb). 2015 Jul;95(4):505-14. Doi: 10.1016/J.Tube.2015.04.006. Epub 2015 May 9. The Fibroblast Growth Factor-2 Arrests Mycobacterium Avium Sp. Paratuberculosis Growth And Immunomodula...
Guo Zy, Sun X, Xu Xl, Zhao Q, Peng J, Wang Y. Neural Regen Res. 2015 Apr;10(4):651-8. Doi: 10.4103/1673-5374.155442. Human Umbilical Cord Mesenchymal Stem Cells Promote Peripheral Nerve Repair Via Paracrine Mechanisms.
Xu Hh, Liu Sh, Guo Qf, Liu Qh, Li Xy. Braz J Med Biol Res. 2013 Sep;46(9):809-14. Doi: 10.1590/1414-431X20132929. Epub 2013 Sep 6. Osteogenesis Induced In Goat Bone Marrow Progenitor Cells By Recombinant Adenovirus Coexpressing Bone Morphogenetic ...
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