Overview
| Product Name |
Human IL-28A ELISA Kit PicoKine™
See more IL-28A/IFN-lambda 2 products |
| Catalog# |
EK0969 |
| Storage & Handling |
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles. |
| Description |
Human IL-28A ELISA Kit PicoKine™ (96 Tests). Quantitate Human IFNL2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).. Sensitivity: 10pg/ml. |
| Cite This Product |
Human IL-28A ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0969)
|
| ELISA Validation |
ELISA Validation Information
|
Product Specs
| Reactivity/Species |
Human |
| Applications |
ELISA
*Our Boster Guarantee covers the use of this product in the above
tested applications.
**For positive and negative control design, consult "Tissue specificity" under
"Protein Target Info" tab. |
| Sample Type |
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
| Sensitivity |
<10 pg/ml |
| Assay Range |
125 pg/ml - 8,000 pg/ml |
| Immunogen/Standard |
Expression system for standard: NS0; Immunogen sequence: V26-V200 |
| Antibody Clonalities |
Capture antibody|Detection antibody
monoclonal antibody from mouse|polyclonal antibody from goat |
| Cross Reactivity |
There is no detectable cross-reactivity with other relevant proteins. |
| Pack Size |
96 wells/kit, with removable strips. |
Gene/Protein Basic Information For IFNL2 (Source: Uniprot.org, NCBI)
| Uniprot Id | Q8IZJ0 |
|---|
| NCBI Gene Id | 282616 |
|---|
| Species Of This Entry | Human |
|---|
| Gene Name | IFNL2 |
|---|
| Protein Name | Interferon lambda-2 |
|---|
| Superfamily | lambda interferon family |
|---|
| Alternative Names | IL-28A/IFN-lambda 2| interleukin-28A; IFN-lambda 2; IL28A; IL-28A; interferon, lambda 2 |
|---|
| Molecular Weight | 22288 |
|---|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on IFNL2, check out the IFNL2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
Want a nice infographic on your next protein? Boster's got them all. All proteins in the human genome, and some, can be found in the Boster Bio gene infographics. Download it and share it with your colleagues and friends. Big size posters available upon request.
In this infographic you will see the following information for IFNL2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. Some times it even contains some opt-ed articles the Boster team on the scientific news and clinical impacts of this protein, though not all have that. Too many proteins, you know.
Take me to the IFNL2 infographic
Kit Components And Assay QC Data Details Of Human IL-28A ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
| Catalog number | Description | Quantity |
| EK0969-CAP |
Anti-Human IFNL2 Pre-coated 96-well strip microplate | 1 |
| EK0969-ST | Human IFNL2 Standard | 2 vials, 10 ng/tube |
| EK0969-DA | Human IFNL2 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
This data is generated from a recent batch of EK0969 Human IL-28A ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
| Concentration (pg/ml) | 0 | 125 | 250 | 500 | 1000 | 2000 | 4000 | 8000 |
| O.D. | 0.034 | 0.092 | 0.152 | 0.272 | 0.491 | 0.949 | 1.594 | 2.331 |
We measured random samples of Human IL-28A ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision |
|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 162 | 1227 | 4870 | 153 | 1196 | 5054 |
| Standard deviation | 6.8 | 67.48 | 282.46 | 5.46 | 78.93 | 343.67 |
| CV (%) | 4.2% | 5.5% | 5.8% | 5.4% | 6.6% | 6.8% |
We measured the performance consistency of Human IL-28A ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|
| Sample 1 | 162 | 184 | 181 | 182 | 177 | 8.87 | 5% |
| Sample 2 | 1227 | 1413 | 1423 | 1411 | 1368 | 81.82 | 5.9% |
| Sample 3 | 4870 | 5325 | 4875 | 4906 | 4994 | 191.59 | 3.8% |
*number of samples for each test n=16.
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
Other Recommended Resources
Here are featured tools and databases that you might find useful.
Total number of citations: 0
1 reviews
"Kit worked great"
Excellent, submitted by Charles Stopford on
2016-08-24
Source: Biocompare.com
"Kit worked great"
--Charles Stopford, UNIVERSITY OF NORTH CARLINA, MICROBIOLOGY AND IMMUNOLOGY, RESEARCH SPECIALIST
| Applications | ELISA |
|---|
| Sample | Primary keratinocytes |
|---|
| Detection | -- |
|---|
"This kit was very straightforward and easy to use. Followed the protocol given in the kit...Straight forward experiment, no tips needed. Kit worked great - positive control was clearly positive. Nothing bad about the kit, although our experimental results were negative. We would buy this kit or similar ELISA kits again from BosterBio."
Contact us with any questions at [email protected] or go to contact us
Questions and answers from customers related to EK0969 Human IL-28A ELISA Kit PicoKine™
8 Related Questions
Question
For this Human IL-28A ELISA Kit PicoKine; has this been tested to have a cross reactivity with human IL-28B?
Verified Customer
Asked: 2019-06-11
Answer
The product Human IL-28A ELISA Kit PicoKine has not been tested to have cross reactivity with human IL-28B. However, human IL-28A have very high sensitivity with human IL-28B at 96%, so there is a possibility that this kit will be also detecting IL-28B.
Boster Scientific Support
Answered: 2019-06-11
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Asked: 2020-11-14
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2020-11-14
Question
Q: if the enzyme conjugated IL-28A antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the IL-28A antigen?
Verified Customer
Asked: 2020-08-05
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2020-08-05
Question
Q: Can IL-28A ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Z. Williams
Asked: 2018-06-01
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2018-06-01
Question
Q: can you suggest the dilution ratio of serum samples for detection of IL-28A in Human serum? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.
Verified Customer
Asked: 2017-10-06
Answer
A: without knowing the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the IL-28A ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2017-10-06
Question
Q: is it okay to use citrate plasma as samples in Human IL-28A Picokine® ELISA Kit (Catalog # EK0969)?
Z. Kaur
Asked: 2017-01-11
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of IL-28A causing disruption of its protein structure. IL-28A may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the IL-28A ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2017-01-11
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification IL-28A using the EK0969 Human IL-28A Picokine® ELISA Kit?
Verified Customer
Asked: 2016-01-27
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples to avoid protein degradation.
Boster Scientific Support
Answered: 2016-01-27
Question
Q: how do I analyze ELISA data? I measured IL-28A level in serum.
Verified Customer
Asked: 2014-07-16
Boster Scientific Support
Answered: 2014-07-16
