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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).|
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Human IL-28A ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human IL-28A ELISA Kit PicoKine™ (96 Tests). Quantitate Human IFNL2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human IL-28A ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0969)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: V26-V200
125 pg/ml - 8,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK0969 is reactive to IFNL2 in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
EK0969 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of IL-28A/IFN-lambda 2
Interferon lambda-2, also called IFNL2 or IL28A is a class II cytokine receptor ligand related to type I interferons. This gene is mapped to 19q13.2. This gene encodes a cytokine distantly related to type I interferons and the IL-10 family. This gene, interleukin 28B (IL28B), and interleukin 29 (IL29) are three closely related cytokine genes that form a cytokine gene cluster. It up-regulates MHC class I antigen expression. It also displays antitumor activity. The ligand/receptor complex seems to signal through the Jak-STAT pathway. This gene seems not to be essential for early virus-activated host defense in vaginal infection, but plays an important role in Toll-like receptor (TLR)-induced antiviral defense. It seems play a significant role in the antiviral immune defense in the intestinal epithelium.
|EK0969-CAP||Anti-Human IFNL2 Pre-coated 96-well strip microplate||1|
|EK0969-ST||Human IFNL2 Standard||2 vials, 10 ng/tube|
|EK0969-DA||Human IFNL2 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human IL-28A PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human IL-28A ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of IL-28A/IFN-lambda 2 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For IFNL2 (Source: Uniprot.Org, NCBI)
lambda interferon family
interleukin-28A; IFN-lambda 2; IL28A; IL-28A; interferon, lambda 2 IFNL2 IFNL2a, IFNL3a, IL-28A, IL28A interferon lambda 2 interferon lambda-2|IFN-lambda-2|cytokine Zcyto20|interleukin 28A (interferon, lambda 2)|interleukin-28A*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on IFNL2, check out the IFNL2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for IFNL2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
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