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Product Info Summary
| SKU: | EK0969 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
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Product info
Product Name
Human IL-28A ELISA Kit PicoKine®
View all IL-28A/IFN-lambda 2 ELISA kits
SKU/Catalog Number
EK0969
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human IL-28A ELISA Kit PicoKine™ (96 Tests). Quantitate Human IFNL2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human IL-28A ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0969)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: V26-V200
Sensitivity
<10 pg/ml
Assay Range
125 pg/ml - 8,000 pg/ml
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0969 is reactive to IFNL2 in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK0969 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of IL-28A/IFN-lambda 2
Interferon lambda-2, also called IFNL2 or IL28A is a class II cytokine receptor ligand related to type I interferons. This gene is mapped to 19q13.2. This gene encodes a cytokine distantly related to type I interferons and the IL-10 family. This gene, interleukin 28B (IL28B), and interleukin 29 (IL29) are three closely related cytokine genes that form a cytokine gene cluster. It up-regulates MHC class I antigen expression. It also displays antitumor activity. The ligand/receptor complex seems to signal through the Jak-STAT pathway. This gene seems not to be essential for early virus-activated host defense in vaginal infection, but plays an important role in Toll-like receptor (TLR)-induced antiviral defense. It seems play a significant role in the antiviral immune defense in the intestinal epithelium.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0969-CAP | Anti-Human IFNL2 Pre-coated 96-well strip microplate | 1 |
| EK0969-ST | Human IFNL2 Standard | 2 vials, 10 ng/tube |
| EK0969-DA | Human IFNL2 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 125 | 250 | 500 | 1000 | 2000 | 4000 | 8000 |
| O.D. | 0.034 | 0.092 | 0.152 | 0.272 | 0.491 | 0.949 | 1.594 | 2.331 |
Data Example Images
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Human IL-28A PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human IL-28A ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 162 | 1227 | 4870 | 153 | 1196 | 5054 |
| Standard deviation | 6.8 | 67.48 | 282.46 | 5.46 | 78.93 | 343.67 |
| CV (%) | 4.2% | 5.5% | 5.8% | 5.4% | 6.6% | 6.8% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of IL-28A/IFN-lambda 2 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 162 | 184 | 181 | 182 | 177 | 8.87 | 5% |
| Sample 2 | 1227 | 1413 | 1423 | 1411 | 1368 | 81.82 | 5.9% |
| Sample 3 | 4870 | 5325 | 4875 | 4906 | 4994 | 191.59 | 3.8% |
Protein Target Info & Infographic
Gene/Protein Information For IFNL2 (Source: Uniprot.Org, NCBI)
Uniprot ID
Q8IZJ0
Gene ID
282616
Gene Name
IFNL2
Full Name
Interferon lambda-2
Weight
22.288kDa
Superfamily
lambda interferon family
Alternative Names
interleukin-28A; IFN-lambda 2; IL28A; IL-28A; interferon, lambda 2 IFNL2 IFNL2a, IFNL3a, IL-28A, IL28A interferon lambda 2 interferon lambda-2|IFN-lambda-2|cytokine Zcyto20|interleukin 28A (interferon, lambda 2)|interleukin-28A
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on IFNL2, check out the IFNL2 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for IFNL2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
Specific Publications For Human IL-28A ELISA Kit PicoKine® (EK0969)
No publications found for EK0969
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
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- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
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1 Reviews For Human IL-28A ELISA Kit PicoKine®
0
"Kit worked great"
Excellent
Source: Biocompare.com
"Kit worked great"
--Charles Stopford, UNIVERSITY OF NORTH CARLINA, MICROBIOLOGY AND IMMUNOLOGY, RESEARCH SPECIALIST
| Applications | ELISA |
|---|---|
| Sample | Primary keratinocytes |
| Detection | -- |
"This kit was very straightforward and easy to use. Followed the protocol given in the kit...Straight forward experiment, no tips needed. Kit worked great - positive control was clearly positive. Nothing bad about the kit, although our experimental results were negative. We would buy this kit or similar ELISA kits again from BosterBio."
Charles Stopford
Verified customer
Submitted 2016-08-24
Customer Q&As
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Find answers in Q&As, reviews.
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8 Customer Q&As for Human IL-28A ELISA Kit PicoKine®
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2020-11-14
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2020-11-14
Question
Q: if the enzyme conjugated IL-28A antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the IL-28A antigen?
Verified Customer
Verified customer
Asked: 2020-08-05
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2020-08-05
Question
For this Human IL-28A ELISA Kit PicoKine; has this been tested to have a cross reactivity with human IL-28B?
Verified Customer
Verified customer
Asked: 2019-06-11
Answer
The product Human IL-28A ELISA Kit PicoKine has not been tested to have cross reactivity with human IL-28B. However, human IL-28A have very high sensitivity with human IL-28B at 96%, so there is a possibility that this kit will be also detecting IL-28B.
Boster Scientific Support
Answered: 2019-06-11
Question
Q: Can IL-28A ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Z. Williams
Verified customer
Asked: 2018-06-01
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2018-06-01
Question
Q: can you suggest the dilution ratio of serum samples for detection of IL-28A in Human serum? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2017-10-06
Answer
A: without knowing the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the IL-28A ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2017-10-06
Question
Q: is it okay to use citrate plasma as samples in Human IL-28A Picokine® ELISA Kit (Catalog # EK0969)?
Z. Kaur
Verified customer
Asked: 2017-01-11
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of IL-28A causing disruption of its protein structure. IL-28A may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the IL-28A ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2017-01-11
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification IL-28A using the EK0969 Human IL-28A Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2016-01-27
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples to avoid protein degradation.
Boster Scientific Support
Answered: 2016-01-27
Question
Q: how do I analyze ELISA data? I measured IL-28A level in serum.
Verified Customer
Verified customer
Asked: 2014-07-16
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2014-07-16
