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Product Info Summary
| SKU: | EK0964 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
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Product info
Product Name
Human IL-29 ELISA Kit PicoKine®
View all IL-29/IFN-lambda 1 ELISA kits
SKU/Catalog Number
EK0964
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human IL-29 ELISA Kit PicoKine™ (96 Tests). Quantitate Human IFNL1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human IL-29 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0964)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: G20-T200
Sensitivity
<10 pg/ml
Assay Range
62.5 pg/ml - 4,000 pg/ml
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0964 is reactive to IFNL1 in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK0964 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of IL-29/IFN-lambda 1
Interferon lambda-1, also called Interleukin-29 (IL-29) is a protein that in humans is encoded by the IL29 gene. IL-29 is a member of the helical cytokine family and is a type III interferon. This gene is mapped to 19q13.2. It is a cytokine with immunomodulatory activity. This gene may play a role in antiviral immunity. It can up-regulates MHC class I antigen expression. It is the ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IFNLR1. The ligand/receptor complex seems to signal through the Jak-STAT pathway. IL-29 plays an important role in host defenses against microbes and its gene is highly upregulated in cells infected with viruses.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0964-CAP | Anti-Human IFNL1 Pre-coated 96-well strip microplate | 1 |
| EK0964-ST | Human IFNL1 Standard | 2 vials, 10 ng/tube |
| EK0964-DA | Human IFNL1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D. | 0.072 | 0.159 | 0.208 | 0.303 | 0.535 | 0.905 | 1.319 | 2.080 |
Data Example Images
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Human IL-29 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human IL-29 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 149 | 759 | 1401 | 149 | 761 | 1382 |
| Standard deviation | 9.38 | 30.36 | 109.27 | 10.43 | 37.28 | 124.38 |
| CV (%) | 4.9% | 4% | 7.8% | 7% | 4.9% | 9% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of IL-29/IFN-lambda 1 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 149 | 146 | 142 | 134 | 142 | 5.62 | 3.9% |
| Sample 2 | 759 | 735 | 743 | 641 | 719 | 46.13 | 6.4% |
| Sample 3 | 1401 | 1245 | 1280 | 1325 | 1312 | 58.31 | 4.4% |
Protein Target Info & Infographic
Gene/Protein Information For IFNL1 (Source: Uniprot.Org, NCBI)
Uniprot ID
Q8IU54
Gene ID
282618
Gene Name
IFNL1
Full Name
Interferon lambda-1
Weight
21.898kDa
Superfamily
lambda interferon family
Alternative Names
cytokine Zcyto21; IFNL1; IFN-lambda 1; IFN-lambda-1; IL29; IL-29; interferon lambda-1; interferon, lambda 1; interleukin 29 (interferon, lambda 1); interleukin-29; ZCYTO21 IFNL1 IL-29, IL29 interferon lambda 1 interferon lambda-1|IFN-lambda-1|cytokine Zcyto21|interleukin 29 (interferon, lambda 1)|interleukin-29
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on IFNL1, check out the IFNL1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for IFNL1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
Specific Publications For Human IL-29 ELISA Kit PicoKine® (EK0964)
EK0964 has been cited in 4 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Increased Serum Levels of IL-28 and IL-29 and the Protective Effect of IL28B rs8099917 Polymorphism in Patients with Hashimoto’s Thyroiditis
IL28B, IL29 and micro-RNA 548 in subacute sclerosing panencephalitis as a rare disease
Fu LX,Chen T,Guo ZP,Cao N,Zhang LW,Zhou PM.Enhanced serum interferon-lambda 1 interleukin-29 levels in patients with psoriasis vulgaris.An Bras Dermatol.2021 May 21:S0365-0596(21)00124-0.doi:10.1016/j.abd.2020.11.007.Epub ahead of print.PMID:34030913.
Species: Human
EK0964 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA
Li P, Yu Q, Ye X, Wang Z, Yang Q. Microbiology. 2011 Sep;157(Pt 9):2639-46. Doi: 10.1099/Mic.0.049148-0. Epub 2011 Jun 23. Lactobacillus S-Layer Protein Inhibition Of Salmonella-Induced Reorganization Of The Cytoskeleton And Activation Of Mapk Sig...
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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11 Customer Q&As for Human IL-29 ELISA Kit PicoKine®
Question
Does EK0964 contain native human biological materials?
Verified customer
Asked: 2021-11-10
Answer
The Human IL-29 ELISA Kit PicoKine® (EK0964) doesn't contain native human biological materials. doesn't contain native human biological materials.
Boster Scientific Support
Answered: 2021-11-11
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
R. Perez
Verified customer
Asked: 2020-07-14
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2020-07-14
Question
Q: how to proceed with the analysis of ELISA data? I measured IL-29 level in serum.
E. Thomas
Verified customer
Asked: 2020-07-04
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2020-07-04
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis IL-29 using the EK0964 Human IL-29 Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2020-03-04
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to avoid protein degradation.
Boster Scientific Support
Answered: 2020-03-04
Question
Q: What is the optimal O.D. value for IL-29 ELISA kit? I performed your IL-29 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain IL-29 even though the O.D. values are not very high?
Y. Evans
Verified customer
Asked: 2020-01-30
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. in your example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this IL-29 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of IL-29 that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2020-01-30
Question
Q: how are cell lysates prepared for use in Picokine® ELISA kits?
W. Nelson
Verified customer
Asked: 2019-11-09
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2019-11-09
Question
Q: is it okay to use citrate plasma as samples in Human IL-29 Picokine® ELISA Kit (Catalog # EK0964)?
J. Harris
Verified customer
Asked: 2019-10-22
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of IL-29 causing degradation of its protein structure. IL-29 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the IL-29 ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2019-10-22
Question
Q: can you suggest the dilution ratio of serum samples for detection of IL-29 in Human serum? I am trying to measure a multiple analytes and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.
L. Surve
Verified customer
Asked: 2019-01-10
Answer
A: having little idea about the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the IL-29 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2019-01-10
Question
Q: if the enzyme conjugated IL-29 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the IL-29 antigen?
M. Robinson
Verified customer
Asked: 2018-08-25
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2018-08-25
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
T. Chen
Verified customer
Asked: 2018-02-24
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2018-02-24
Question
Q: which procedure should I follow in order to thaw whole blood sample for IL-29 ELISA after freezing?
Verified Customer
Verified customer
Asked: 2018-01-02
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test IL-29 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2018-01-02
