Overview
| Product Name |
Human IL-29 ELISA Kit PicoKine™
See more IL-29/IFN-lambda 1 products |
| Catalog# |
EK0964 |
| Storage & Handling |
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles. |
| Description |
Human IL-29 ELISA Kit PicoKine™ (96 Tests). Quantitate Human IFNL1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).. Sensitivity: 10pg/ml. |
| Cite This Product |
Human IL-29 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0964)
|
| ELISA Validation |
ELISA Validation Information
|
Product Specs
| Reactivity/Species |
Human |
| Applications |
ELISA
*Our Boster Guarantee covers the use of this product in the above
tested applications.
**For positive and negative control design, consult "Tissue specificity" under
"Protein Target Info" tab. |
| Sample Type |
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
| Sensitivity |
<10 pg/ml |
| Assay Range |
62.5 pg/ml - 4,000 pg/ml |
| Immunogen/Standard |
Expression system for standard: NS0; Immunogen sequence: G20-T200 |
| Antibody Clonalities |
Capture antibody|Detection antibody
monoclonal antibody from mouse|polyclonal antibody from goat |
| Cross Reactivity |
There is no detectable cross-reactivity with other relevant proteins. |
| Pack Size |
96 wells/kit, with removable strips. |
Gene/Protein Basic Information For IFNL1 (Source: Uniprot.org, NCBI)
| Uniprot Id | Q8IU54 |
|---|
| NCBI Gene Id | 282618 |
|---|
| Species Of This Entry | Human |
|---|
| Gene Name | IFNL1 |
|---|
| Protein Name | Interferon lambda-1 |
|---|
| Superfamily | lambda interferon family |
|---|
| Alternative Names | IL-29/IFN-lambda 1|cytokine Zcyto21; IFNL1; IFN-lambda 1; IFN-lambda-1; IL29; IL-29; interferon lambda-1; interferon, lambda 1; interleukin 29 (interferon, lambda 1); interleukin-29; ZCYTO21 |
|---|
| Molecular Weight | 21898 |
|---|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on IFNL1, check out the IFNL1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
Want a nice infographic on your next protein? Boster's got them all. All proteins in the human genome, and some, can be found in the Boster Bio gene infographics. Download it and share it with your colleagues and friends. Big size posters available upon request.
In this infographic you will see the following information for IFNL1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. Some times it even contains some opt-ed articles the Boster team on the scientific news and clinical impacts of this protein, though not all have that. Too many proteins, you know.
Take me to the IFNL1 infographic
Kit Components And Assay QC Data Details Of Human IL-29 ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
| Catalog number | Description | Quantity |
| EK0964-CAP |
Anti-Human IFNL1 Pre-coated 96-well strip microplate | 1 |
| EK0964-ST | Human IFNL1 Standard | 2 vials, 10 ng/tube |
| EK0964-DA | Human IFNL1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
This data is generated from a recent batch of EK0964 Human IL-29 ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
| Concentration (pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D. | 0.072 | 0.159 | 0.208 | 0.303 | 0.535 | 0.905 | 1.319 | 2.280 |
We measured random samples of Human IL-29 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision |
|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 149 | 759 | 1401 | 149 | 761 | 1382 |
| Standard deviation | 9.38 | 30.36 | 109.27 | 10.43 | 37.28 | 124.38 |
| CV (%) | 4.9% | 4% | 7.8% | 7% | 4.9% | 9% |
We measured the performance consistency of Human IL-29 ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|
| Sample 1 | 149 | 146 | 142 | 134 | 142 | 5.62 | 3.9% |
| Sample 2 | 759 | 735 | 743 | 641 | 719 | 46.13 | 6.4% |
| Sample 3 | 1401 | 1245 | 1280 | 1325 | 1312 | 58.31 | 4.4% |
*number of samples for each test n=16.
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
Other Recommended Resources
Here are featured tools and databases that you might find useful.
Total number of citations: 1
| Li P, Yu Q, Ye X, Wang Z, Yang Q. Microbiology. 2011 Sep;157(Pt 9):2639-46. Doi: 10.1099/Mic.0.049148-0. Epub 2011 Jun 23. Lactobacillus S-Layer Protein Inhibition Of Salmonella-Induced Reorganization Of The Cytoskeleton And Activation Of Mapk Sig... |
PubMed ID 21700664 |
Contact us with any questions at [email protected] or go to contact us
Questions and answers from customers related to EK0964 Human IL-29 ELISA Kit PicoKine™
10 Related Questions
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
R. Perez
Asked: 2020-07-14
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2020-07-14
Question
Q: how to proceed with the analysis of ELISA data? I measured IL-29 level in serum.
E. Thomas
Asked: 2020-07-04
Boster Scientific Support
Answered: 2020-07-04
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis IL-29 using the EK0964 Human IL-29 Picokine® ELISA Kit?
Verified Customer
Asked: 2020-03-04
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to avoid protein degradation.
Boster Scientific Support
Answered: 2020-03-04
Question
Q: What is the optimal O.D. value for IL-29 ELISA kit? I performed your IL-29 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain IL-29 even though the O.D. values are not very high?
Y. Evans
Asked: 2020-01-30
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. in your example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this IL-29 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of IL-29 that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2020-01-30
Question
Q: how are cell lysates prepared for use in Picokine® ELISA kits?
W. Nelson
Asked: 2019-11-09
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2019-11-09
Question
Q: is it okay to use citrate plasma as samples in Human IL-29 Picokine® ELISA Kit (Catalog # EK0964)?
J. Harris
Asked: 2019-10-22
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of IL-29 causing degradation of its protein structure. IL-29 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the IL-29 ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2019-10-22
Question
Q: can you suggest the dilution ratio of serum samples for detection of IL-29 in Human serum? I am trying to measure a multiple analytes and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.
L. Surve
Asked: 2019-01-10
Answer
A: having little idea about the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the IL-29 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2019-01-10
Question
Q: if the enzyme conjugated IL-29 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the IL-29 antigen?
M. Robinson
Asked: 2018-08-25
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2018-08-25
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
T. Chen
Asked: 2018-02-24
Boster Scientific Support
Answered: 2018-02-24
Question
Q: which procedure should I follow in order to thaw whole blood sample for IL-29 ELISA after freezing?
Verified Customer
Asked: 2018-01-02
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test IL-29 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2018-01-02
