Overview
| Product Name |
Human IL-29 ELISA Kit PicoKine™
See all IL-29/IFN-lambda 1 primary antibodies, ELISA kits and proteins |
| SKU/Catalog Number |
EK0964 |
| Storage & Handling |
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.) |
| Description |
Detect Human Il 29/IFNL1 with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Il 29/IFNL1 tissue specificity: |
| Cite This Product |
Human IL-29 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0964)
|
Product Specs
| Reactivity/Species |
Human |
| Applications |
ELISA
*Our Boster Guarantee covers the use of this product in the above
tested applications.
**For positive and negative control design, consult "Tissue specificity" under
"Protein Target Info" tab. |
| Sample Type |
cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). |
| Sensitivity |
<10pg/ml |
| Assay Range |
62.5pg/ml-4000pg/ml |
| Immunogen/Standard |
Expression system for standard: NSO; Immunogen sequence: G20-T200 |
| Antibody Clonalities |
Capture antibody|Detection antibody
monoclonal antibody from mouse|polyclonal antibody from goat |
| Cross Reactivity |
There is no detectable cross-reactivity with other relevant proteins. |
| Pack Size |
96wells/kit, with removable strips. |
Gene/Protein Basic Information For IFNL1 (Source: Uniprot.org, NCBI)
| Uniprot Id | Q8IU54 |
|---|
| NCBI Gene Id | 282618 |
|---|
| Species Of This Entry | Human |
|---|
| Gene Name | IFNL1 |
|---|
| Protein Name | Interferon lambda-1 |
|---|
| Superfamily | lambda interferon family |
|---|
| Alternative Names | IL-29/IFN-lambda 1|cytokine Zcyto21; IFNL1; IFN-lambda 1; IFN-lambda-1; IL29; IL-29; interferon lambda-1; interferon, lambda 1; interleukin 29 (interferon, lambda 1); interleukin-29; ZCYTO21 |
|---|
| Molecular Weight | 21898 |
|---|
| Post Tranlational Modifications | Disulfide bond; Glycoprotein |
|---|
| Subcellular Localization | Secreted. |
|---|
| Gene Location | 19q13.2, on Chromasome 19, gene sequence: NC_000019.10 (39296407..39298673) |
|---|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For IFNL1 (Source: Uniprot.org, NCBI)
| Protein Function | Cytokine with antiviral, antitumour and immunomodulatory activities. Plays a critical role in the antiviral host defense, predominantly in the epithelial tissues. Acts as a ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IFNLR1, and receptor engagement leads to the activation of the JAK/STAT signaling pathway resulting in the expression of IFN- stimulated genes (ISG), which mediate the antiviral state. Has a restricted receptor distribution and therefore restricted targets: is primarily active in epithelial cells and this cell type- selective action is because of the epithelial cell-specific expression of its receptor IFNLR1. Exerts an immunomodulatory effect by up-regulating MHC class I antigen expression. |
|---|
| Background | Interferon lambda-1, also called Interleukin-29(IL-29) is a protein that in humans is encoded by the IL29 gene. IL-29 is a member of the helical cytokine family and is a type III interferon. This gene is mapped to 19q13.2. It is a cytokine with immunomodulatory activity. This gene may play a role in antiviral immunity. It can up-regulates MHC class I antigen expression. It is the ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IFNLR1. The ligand/receptor complex seems to signal through the Jak-STAT pathway. IL-29 plays an important role in host defenses against microbes and its gene is highly upregulated in cells infected with viruses. |
|---|
| Scientific References | PMID: 12469119 by Sheppard P., et al. IL-28, IL-29 and their class II cytokine receptor IL-28R.
PMID: 12483210 by Kotenko S.V., et al. IFN-lambdas mediate antiviral protection through a distinct class II cytokine receptor complex. |
|---|
Kit Components And Assay QC Data Details Of Human IL-29 ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
| Catalog number | Description | Quantity |
| EK0964-CAP | 96-well plate precoated with anti-Human IL-29/IFN-lambda 1 antibody | 1 |
| EK0964-ST | lyophilized recombinant Human IL-29/IFN-lambda 1 standard | 10ng/tube |
| EK0964-DA | biotinylated anti-Human IL-29/IFN-lambda 1 antibody | 130ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 130ul |
| AR1106-1 | sample diluent buffer | 30ml |
| AR1106-2 | antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EK0964 Human IL-29 ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
| Concentration(pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D. | 0.072 | 0.159 | 0.208 | 0.303 | 0.535 | 0.905 | 1.319 | 2.280 |
We measured random samples of Human IL-29 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision |
|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 149 | 759 | 1401 | 149 | 761 | 1382 |
| Standard deviation | 9.38 | 30.36 | 109.27 | 10.43 | 37.28 | 124.38 |
| CV(%) | 4.9% | 4% | 7.8% | 7% | 4.9% | 9% |
We measured the performance consistency of Human IL-29 ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|
| Sample 1 | 149 | 146 | 142 | 134 | 142 | 5.62 | 3.9% |
| Sample 2 | 759 | 735 | 743 | 641 | 719 | 46.13 | 6.4% |
| Sample 3 | 1401 | 1245 | 1280 | 1325 | 1312 | 58.31 | 4.4% |
*number of samples for each test n=16.
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
See all IL-29/IFN-lambda 1 primary antibodies,
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Total number of citations: 1
| Li P, Yu Q, Ye X, Wang Z, Yang Q. Microbiology. 2011 Sep;157(Pt 9):2639-46. Doi: 10.1099/Mic.0.049148-0. Epub 2011 Jun 23. Lactobacillus S-Layer Protein Inhibition Of Salmonella-Induced Reorganization Of The Cytoskeleton And Activation Of Mapk Sig... |
PubMed ID 21700664 |
