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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).|
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Human IL-29 ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human IL-29 ELISA Kit PicoKine™ (96 Tests). Quantitate Human IFNL1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human IL-29 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0964)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: G20-T200
62.5 pg/ml - 4,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK0964 is reactive to IFNL1 in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
EK0964 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of IL-29/IFN-lambda 1
Interferon lambda-1, also called Interleukin-29 (IL-29) is a protein that in humans is encoded by the IL29 gene. IL-29 is a member of the helical cytokine family and is a type III interferon. This gene is mapped to 19q13.2. It is a cytokine with immunomodulatory activity. This gene may play a role in antiviral immunity. It can up-regulates MHC class I antigen expression. It is the ligand for the heterodimeric class II cytokine receptor composed of IL10RB and IFNLR1. The ligand/receptor complex seems to signal through the Jak-STAT pathway. IL-29 plays an important role in host defenses against microbes and its gene is highly upregulated in cells infected with viruses.
|EK0964-CAP||Anti-Human IFNL1 Pre-coated 96-well strip microplate||1|
|EK0964-ST||Human IFNL1 Standard||2 vials, 10 ng/tube|
|EK0964-DA||Human IFNL1 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human IL-29 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human IL-29 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of IL-29/IFN-lambda 1 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For IFNL1 (Source: Uniprot.Org, NCBI)
lambda interferon family
cytokine Zcyto21; IFNL1; IFN-lambda 1; IFN-lambda-1; IL29; IL-29; interferon lambda-1; interferon, lambda 1; interleukin 29 (interferon, lambda 1); interleukin-29; ZCYTO21 IFNL1 IL-29, IL29 interferon lambda 1 interferon lambda-1|IFN-lambda-1|cytokine Zcyto21|interleukin 29 (interferon, lambda 1)|interleukin-29*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on IFNL1, check out the IFNL1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for IFNL1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
EK0964 has been cited in 4 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Increased Serum Levels of IL-28 and IL-29 and the Protective Effect of IL28B rs8099917 Polymorphism in Patients with Hashimoto’s Thyroiditis
IL28B, IL29 and micro-RNA 548 in subacute sclerosing panencephalitis as a rare disease
Fu LX,Chen T,Guo ZP,Cao N,Zhang LW,Zhou PM.Enhanced serum interferon-lambda 1 interleukin-29 levels in patients with psoriasis vulgaris.An Bras Dermatol.2021 May 21:S0365-0596(21)00124-0.doi:10.1016/j.abd.2020.11.007.Epub ahead of print.PMID:34030913.
EK0964 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA
Li P, Yu Q, Ye X, Wang Z, Yang Q. Microbiology. 2011 Sep;157(Pt 9):2639-46. Doi: 10.1099/Mic.0.049148-0. Epub 2011 Jun 23. Lactobacillus S-Layer Protein Inhibition Of Salmonella-Induced Reorganization Of The Cytoskeleton And Activation Of Mapk Sig...
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