Human IL-8/Interleukin-8/CXCL8 ELISA Kit PicoKine™

Human IL-8/Interleukin-8/CXCL8 ELISA Kit PicoKine™ (96 Tests). Quantitate Human CXCL8 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 1pg/ml. Cited in 70 publication(s).

Product Info Summary

SKU: EK0413
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Product Name

Human IL-8/Interleukin-8/CXCL8 ELISA Kit PicoKine™

See all CXCL8/IL-8 products

SKU/Catalog Number

EK0413

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human IL-8/Interleukin-8/CXCL8 ELISA Kit PicoKine™ (96 Tests). Quantitate Human CXCL8 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 1pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human IL-8/Interleukin-8/CXCL8 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0413)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: S28-S99

Sensitivity

<1 pg/ml

Assay Range

15.6 pg/ml - 1,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0413 is reactive to CXCL8 in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Application Guarantee

EK0413 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0413-CAP Anti-Human CXCL8 Pre-coated 96-well strip microplate 1
EK0413-ST Human CXCL8 Standard 2 vials, 10 ng/tube
EK0413-DA Human CXCL8 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015.631.262.51252505001000
O.D.0.0000.0820.1490.2810.5560.9361.5942.071

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect CXCL8/IL-8, Dilution ratio of 1:1, concentration in serum is target in serum is below detection limit..

Some articles we found to cite concentrations of CXCL8/IL-8 in samples: 15529589 (Pubmed IDs).

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human IL-8/Interleukin-8/CXCL8 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)16662341663230
Standard deviation0.722.8314.270.83.8417.48
CV (%)4.5%4.3%6.1%5%6.1%7.6%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of CXCL8/IL-8 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 116161517160.74.3%
Sample 266677274693.344.8%
Sample 323424925522123913.255.5%
*number of samples for each test n=16.

Gene/Protein Information For CXCL8 (Source: Uniprot.Org, NCBI)

Uniprot ID

P10145

Gene ID

3576

Gene Name

CXCL8

Full Name

Interleukin-8

Weight

11.098kDa

Superfamily

intercrine alpha (chemokine CxC) family

Alternative Names

3-10C; AMCF-I; C-X-C motif chemokine 8; CXCL8; CXCL8SCYB8; Emoctakin; GCP1; GCP-1TSG-1; IL8; IL-8; interleukin 8; K60; LAI; LECT; LUCT; LYNAP; MDNCF; MDNCFb-ENAP; member 8; MONAP; MONAPGCP1; NAF; NAP1; NAP-1NAP1; NCF; Neutrophil-activating protein 1; Protein 3-10C; T cell chemotactic factor; T-cell chemotactic factor; TCF; TSG1 CXCL8 GCP-1, GCP1, IL8, LECT, LUCT, LYNAP, MDNCF, MONAP, NAF, NAP-1, NAP1, SCYB8 C-X-C motif chemokine ligand 8 interleukin-8|T-cell chemotactic factor|alveolar macrophage chemotactic factor I|beta endothelial cell-derived neutrophil activating peptide|beta-thromboglobulin-like protein|chemokine (C-X-C motif) ligand 8|emoctakin|granulocyte chemotactic protein 1|interleukin 8|lung giant cell carcinoma-derived chemotactic protein|lymphocyte derived neutrophil activating peptide|monocyte-derived neutrophil chemotactic factor|monocyte-derived neutrophil-activating peptide|neutrophil-activating peptide 1|small inducible cytokine subfamily B, member 8|tumor necrosis factor-induced gene 1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CXCL8, check out the CXCL8 Infographic

CXCL8 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CXCL8: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0413 has been cited in 70 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

IRAK1 is a therapeutic target that drives breast cancer metastasis and resistance to paclitaxel
Species: Human

IRAK1 is a therapeutic target that drives breast cancer metastasis and resistance to paclitaxel
Species: Human

Bone-Targeted Nanoplatform Combining Zoledronate and Photothermal Therapy To Treat Breast Cancer Bone Metastasis
Species: Human

Boyang Sun,Siqi Ying,Qian Ma,Han Li,Jie Li,Jinlin Song,Metformin ameliorates HMGB1-mediated oxidative stress through mTOR/AMPK pathway in experimental periodontitis,Genes & Diseases,2021,,ISSN 2352-3042,https://doi.org/10.1016/j.gendis.2021.06.003.
Species: Human,Mouse
EK0413 usage in article: APP: ELISA, SAMPLE:HPDLCS, DILUTION:NA

Stellavato,A.;Restaino,O.F.;Vassallo,V.;Cassese,E.;Finamore,R.;Ruosi,C.;Schiraldi,C.Chondroitin Sulfate in USA Dietary Supplements in Comparison to Pharma Grade Products: Analytical Fingerprint and Potential Anti-Inflammatory Effect on Human Osteoartritic Chondrocytes and Synoviocytes.Pharmaceutics 2021,13,737.https://doi.org/10.3390/pharmaceutics13050737
Species: Human
EK0413 usage in article: APP:ELISA, SAMPLE:CHONDROCYTES, DILUTION:NA

Erica Costantini,Bruna Sinjari,Katia Falasca,Marcella Reale,Sergio Caputi,Srinivas Jagarlapodii,Giovanna Murmura," Assessment of the Vanillin Anti-Inflammatory and Regenerative Potentials in Inflamed Primary Human Gingival Fibroblast",Mediators of Inflammation,vol.2021,Article ID 5562340,9 pages,2021. https://doi.org/10.1155/2021/5562340
Species: Human
EK0413 usage in article: APP:ELISA, SAMPLE:HGF CELL, DILUTION:NA

Ying-nan Ju,Qi-hang Tai,Guang-xiao Xu,Xiao-qing Zhao,Hai-bin Sun,Wei Gao,"Diannexin Can Ameliorate Acute Respiratory Distress Syndrome in Rats by Promoting Heme Oxygenase-1 Expression",Mediators of Inflammation,vol.2021,Article ID 1946384,10 pages,2021.https://doi.org/10.1155/2021/1946384
Species: Rat
EK0413 usage in article: APP:ELISA, SAMPLE:BALF AND SERUM, DILUTION:NA

Li J,Xie Y,Zhao P,Qin Y,Oliver BG,Tian Y,Li S,Wang M,Liu X. A chinese herbal formula ameliorates COPD by inhibiting the inflammatory response via downregulation of p65, JNK, and p38. Phytomedicine. 2021 Jan 20;83:153475.doi:10.1016/j.phymed.2021.153475.Ep
Species: Human,Rat
EK0413 usage in article: APP:ELISA, SAMPLE:SERUM AND BALF, DILUTION:NA

Li Y,Yuan R,Ren T,Yang B,Miao H,Liu L,Li Y,Cai C,Yang Y,Hu Y,Jiang C,Xu Q,Zhang Y,Liu Y.Role of Sciellin in gallbladder cancer proliferation and formation of neutrophil extracellular traps.Cell Death Dis.2021 Jan 6;12(1):30.doi:10.1038/s41419-020-03286-z.
Species: Human,Mouse
EK0413 usage in article: APP:ELISA, SAMPLE:GBC CELLS, DILUTION:NA

Qing Zhou,Peiyu Jin,Jieyu Liu,Sihao Li,Weijue Liu,Shuhua Xi,HER2 overexpression triggers the IL-8 to promote arsenic-induced EMT and stem cell-like phenotypes in human bladder epithelial cells, Ecotoxicology and Environmental Safety, Volume 208, 2021,1116
Species: Human
EK0413 usage in article: APP:ELISA, SAMPLE:T-SV-HUC-1 CELLS, DILUTION:NA

Yang S,Wang H,Qin C,Sun H,Han Y.Up-regulation of CXCL8 expression is associated with a poor prognosis and enhances tumor cell malignant behaviors in liver cancer. Biosci Rep.2020 Aug 28;40(8):BSR20201169.doi: 10.1042/BSR20201169.PMID:32766720;PMCID:PMC744
Species: Human
EK0413 usage in article: APP:ELISA, SAMPLE:HEPG 2 CELL, DILUTION:NA

Liao H,Zhang H,Xiang J,Chen G,Cao Z.The effect of the surgical microscope on the outcome of root scaling. Am J Transl Res. 2020 Nov 15;12(11):7199-7210.PMID: 33312360;PMCID:PMC7724328.
Species: Human
EK0413 usage in article: APP:ELISA, SAMPLE:PDLCS, DILUTION:NA

Asila A, Wang Y, Miao D, Yang X, Kaisaer Y, Ma L, Lv G, Yang C. SNHG16/miR-485-5p/BMP7 axis modulates osteogenic differentiation of human bone marrow-derived mesenchymal stem cells. J Gene Med. 2020 Nov 11:e3296. doi: 10.1002/jgm.3296. Epub ahead of print
Species: Human
EK0413 usage in article: APP:ELISA, SAMPLE:HBMSCS, DILUTION:NA

Ginsenoside Rg3 inhibits the senescence of prostate stromal cells through down-regulation of interleukin 8 expression

FOXQ1 regulates senescence-associated inflammation via activation of SIRT1 expression

Randomized, double-blind, placebo-controlled superiority trial of the Yiqigubiao pill for the treatment of patients with chronic obstructive pulmonary disease at a stable stage

Evaluation of optimized continuous venovenous hemodiafiltration therapy efficiency in severe burn patients with sepsis

Pharmacological Inactivation of Src Family Kinases Inhibits LPS-Induced TNF-? Production in PBMC of Patients with Beh?et's Disease

A 3D printed nano bone matrix for characterization of breast cancer cell and osteoblast interactions

Characteristics of CD4+CD25+Foxp3+?regulatory T cells in patients with multiple organ dysfunction syndrome

C/EBP ? Mediates Endoplasmic Reticulum Stress Regulated Inflammatory Response and Extracellular Matrix Degradation in LPS-Stimulated Human Periodontal Ligament Cells

Inhibition of zymosan-induced cytokine and chemokine expression in human corneal fibroblasts by triptolide

Bclaf1 is an important NF-?B signaling transducer and C/EBP? regulator in DNA damage-induced senescence

IRAK1 is a therapeutic target that drives breast cancer metastasis and resistance to paclitaxel

Metabolic changes of H2S in smokers and patients of COPD which might involve in inflammation, oxidative stress and steroid sensitivity

Inflammatory response of macrophages cultured with Helicobacter pylori strains was regulated by miR-155

Evaluation of the Inflammatory Response in Macrophages Stimulated with Exosomes Secreted by?Mycobacterium avium-Infected Macrophages

Hydrogen Sulfide Protects against Chemical Hypoxia-Induced Cytotoxicity and Inflammation in HaCaT Cells through Inhibition of ROS/NF-?B/COX-2 Pathway

Huang N, Xu Y, Zhou H, Lin D, Zhang B, Zhang Y, Pan D, Tao L, Liu X, Shen X. Med Sci Monit. 2017 Oct 4;23:4760-4767. Essential Oil from Fructus Alpiniae Zerumbet Protects Human Umbilical Vein Endothelial Cells In Vitro from Injury Induced by High ...

Sun Y, Pan J, Zhang N, Wei W, Yu S, Ai L. Sci Rep. 2017 Dec 22;7(1):18079. doi: 10.1038/s41598-017-18056-9. Knockdown of long non-coding RNA H19 inhibits multiple myeloma cell growth via NF-κB pathway

Sun X, Shi B, Zheng H, Min L, Yang J, Li X, Liao X, Huang W, Zhang M, Xu S, Zhu Z, Cui H, Liu X. Cell Death Dis. 2018 Feb 15;9(3):260. doi: 10.1038/s41419-018-0303-9. Senescence-associated secretory factors induced by cisplatin in melanoma cells p...

Zhang X, Zheng Z, Shu B, Liu X, Zhang Z, Liu Y, Bai B, Hu Q, Mao P, Wang H. J Virol. 2013 Nov;87(22):12407-21. Doi: 10.1128/Jvi.02090-13. Epub 2013 Sep 11. Human Astrocytic Cells Support Persistent Coxsackievirus B3 Infection.

Li J, Yang L, Yao Q, Li Y, Tian Y, Li S, Jiang S, Wang Y, Li X, Guo Z. Evid Based Complement Alternat Med. 2014;2014:381976. Doi: 10.1155/2014/381976. Epub 2014 May 14. Effects And Mechanism Of Bufei Yishen Formula In A Rat Chronic Obstructive Pul...

Dai Jp, Zhao Xf, Zeng J, Wan Qy, Yang Jc, Li Wz, Chen Xx, Wang Gf, Li Ks. Plos One. 2013 Apr 16;8(4):E61026. Doi: 10.1371/Journal.Pone.0061026. Print 2013. Drug Screening For Autophagy Inhibitors Based On The Dissociation Of Beclin1-Bcl2 Complex U...

Xie Xj, Ye Yf, Zhou L, Xie Hy, Jiang Gp, Feng Xw, He Y, Xie Qf, Zheng Ss. J Zhejiang Univ Sci B. 2010 Nov;11(11):819-27. Doi: 10.1631/Jzus.B1000030. Th17 Promotes Acute Rejection Following Liver Transplantation In Rats.

Dong Sj, Zhong Yq, Lu Wt, Li Gh, Jiang Hl, Mao B. Inflammation. 2015 Aug;38(4):1493-501. Doi: 10.1007/S10753-015-0124-2. Baicalin Inhibits Lipopolysaccharide-Induced Inflammation Through Signaling Nf-??b Pathway In Hbe16 Airway Epithelial Cells.

Yu Q, Wang Z, Yang Q. J Dairy Sci. 2012 Oct;95(10):5580-7. Doi: 10.3168/Jds.2012-5540. Epub 2012 Aug 9. Lactobacillus Amylophilus D14 Protects Tight Junction From Enteropathogenic Bacteria Damage In Caco-2 Cells.

Li Ff, Shen J, Shen Hj, Zhang X, Cao R, Zhang Y, Qui Q, Lin Xx, Xie Yc, Zhang Lh, Jia Yl, Dong Xw, Jiang Jx, Bao Mj, Zhang S, Ma Wj, Wu Xm, Shen H, Xie Qm, Ke Y. J Immunol. 2012 Sep 15;189(6):3159-67. Doi: 10.4049/Jimmunol.1200197. Epub 2012 Aug 1...

He L, Zhang E, Shi J, Li X, Zhou K, Zhang Q, Le Ad, Tang X. Cancer Chemother Pharmacol. 2013 Mar;71(3):713-25. Doi: 10.1007/S00280-012-2063-Z. Epub 2013 Jan 6. (-)-Epigallocatechin-3-Gallate Inhibits Human Papillomavirus (Hpv)-16 Oncoprotein-Induc...

Bao Mh, Zhang Yw, Lou Xy, Xiao Y, Cheng Y, Zhou Hh. Can J Physiol Pharmacol. 2014 Apr;92(4):299-306. Doi: 10.1139/Cjpp-2013-0322. Epub 2014 Jan 10. Puerarin Protects Endothelial Cells From Oxidized Low Density Lipoprotein Induced Injuries Via The ...

Zhai X, Han Q, Shan Z, Qu X, Guo L, Zhou Y. Mol Med Rep. 2014 Dec;10(6):3052-8. Doi: 10.3892/Mmr.2014.2575. Epub 2014 Sep 18. Dual Specificity Phosphatase 6 Suppresses The Growth And Metastasis Of Prostate Cancer Cells.

Wang H, Zhuo Y, Hu X, Shen W, Zhang Y, Chu T. Biochem Biophys Res Commun. 2015 Mar 6;458(2):268-73. Doi: 10.1016/J.Bbrc.2015.01.097. Epub 2015 Feb 3. Cd147 Deficiency Blocks Il-8 Secretion And Inhibits Lung Cancer-Induced Osteoclastogenesis.

Pei X, Li M, Zhan J, Yu Y, Wei X, Guan L, Aydin H, Elson P, Zhou M, He H, Zhang H. Plos One. 2015 Apr 28;10(4):E0124338. Doi: 10.1371/Journal.Pone.0124338. Ecollection 2015. Enhanced Imp3 Expression Activates Nf-??b Pathway And Promotes Renal Cell...

Xiao X, Gang Y, Wang H, Wang J, Zhao L, Xu L, Liu Z. Biochem Biophys Res Commun. 2015 Feb 6;457(2):221-6. Doi: 10.1016/J.Bbrc.2014.12.091. Epub 2014 Dec 27. Double-Stranded Rna Transcribed From Vector-Based Oligodeoxynucleotide Acts As Transcripti...

Li X, Qian D, Ju F, Wang B. Oncol Lett. 2015 Jan;9(1):365-370. Epub 2014 Oct 15. Upregulation Of Toll-Like Receptor 2 Expression In Colorectal Cancer Infected By Human Cytomegalovirus.

Zhang E, Feng X, Liu F, Zhang P, Liang J, Tang X. Plos One. 2014 Jul 24;9(7):E103440. Doi: 10.1371/Journal.Pone.0103440. Ecollection 2014. Roles Of Pi3K/Akt And C-Jun Signaling Pathways In Human Papillomavirus Type 16 Oncoprotein-Induced Hif-1??, ...

Chen L, Xiao Z, Meng Y, Zhao Y, Han J, Su G, Chen B, Dai J. Biomaterials. 2012 Feb;33(5):1437-44. Doi: 10.1016/J.Biomaterials.2011.10.056. Epub 2011 Nov 10. The Enhancement Of Cancer Stem Cell Properties Of Mcf-7 Cells In 3D Collagen Scaffolds For...

Liu Lj, Zhang J, Xiao Zf, Dai B, Sun My, Chen L, Chen B. J Biomed Mater Res B Appl Biomater. 2014 May;102(4):772-80. Doi: 10.1002/Jbm.B.33058. Epub 2013 Oct 21. Three-Dimensional Collagen Scaffold Enhances The Human Adenoid Cystic Carcinoma Cancer...

Ping C, Yongping Z, Minmin Q, Weiyan Y, Yaozong Y. Dig Dis Sci. 2010 Jun;55(6):1599-609. Doi: 10.1007/S10620-009-0909-Y. Epub 2009 Aug 13. Activated Protein C Improves The Severity Of Severe Acute Pancreatitis Via Up-Regulating The Expressions Of ...

Zhang H, Yin G, Jiang H, Zhang C. J Int Med Res. 2013 Aug;41(4):1179-86. Doi: 10.1177/0300060513488503. Epub 2013 Jun 14. High-Dose N-Acetylcysteine Decreases Silica-Induced Lung Fibrosis In The Rat.

Li Mc, Yang G, Zhou Xd, Tselluyko S, Perelman Jm. Int J Mol Med. 2014 Jan;33(1):83-90. Doi: 10.3892/Ijmm.2013.1535. Epub 2013 Oct 23. The Pathophysiological Mechanisms Underlying Mucus Hypersecretion Induced By Cold Temperatures In Cigarette Smoke...

Ren Dl, Li Yj, Hu Bb, Wang H, Hu B. J Pineal Res. 2015 May;58(4):452-60. Doi: 10.1111/Jpi.12230. Epub 2015 Mar 26. Melatonin Regulates The Rhythmic Migration Of Neutrophils In Live Zebrafish.

Liu L, Li C, Xiang J, Dong W, Cao Z. J Periodontal Res. 2013 Oct;48(5):615-22. Doi: 10.1111/Jre.12047. Epub 2013 Feb 27. Over-Expression And Potential Role Of Cyclophilin A In Human Periodontitis.

Capsoni F, Ongari Am, Lonati C, Accetta R, Gatti S, Catania A. Bmc Musculoskelet Disord. 2015 Jun 21;16:154. Doi: 10.1186/S12891-015-0615-1. ??-Melanocyte-Stimulating-Hormone (??-Msh) Modulates Human Chondrocyte Activation Induced By Proinflammato...

Li P, Yu Q, Ye X, Wang Z, Yang Q. Microbiology. 2011 Sep;157(Pt 9):2639-46. Doi: 10.1099/Mic.0.049148-0. Epub 2011 Jun 23. Lactobacillus S-Layer Protein Inhibition Of Salmonella-Induced Reorganization Of The Cytoskeleton And Activation Of Mapk Sig...

Ma Yj, He M, Han Ja, Yang L, Ji Xy. Scand J Immunol. 2013 Oct;78(4):387-93. Doi: 10.1111/Sji.12097. A Clinical Study Of Hbsag-Activated Dendritic Cells And Cytokine-Induced Killer Cells During The Treatment For Chronic Hepatitis B.

Li Y, Li Sy, Li Js, Deng L, Tian Yg, Jiang Sl, Wang Y, Wang Yy. Biol Pharm Bull. 2012;35(10):1752-60. Epub 2012 Aug 3. A Rat Model For Stable Chronic Obstructive Pulmonary Disease Induced By Cigarette Smoke Inhalation And Repetitive Bacterial Infe...

Li G, He L, Zhang E, Shi J, Zhang Q, Le Ad, Zhou K, Tang X. Cancer Lett. 2011 Dec 8;311(2):160-70. Doi: 10.1016/J.Canlet.2011.07.012. Epub 2011 Jul 22. Overexpression Of Human Papillomavirus (Hpv) Type 16 Oncoproteins Promotes Angiogenesis Via Enh...

Hu Mm, Yang Q, Zhang J, Liu Sm, Zhang Y, Lin H, Huang Zf, Wang Yy, Zhang Xd, Zhong B, Shu Hb. Proc Natl Acad Sci U S A. 2014 Jan 28;111(4):1509-14. Doi: 10.1073/Pnas.1318227111. Epub 2014 Jan 13. Trim38 Inhibits Tnf??- And Il-1??-Triggered Nf-??b ...

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Hu Y, Chen X, Duan H, Hu Y, Mu X. Immunopharmacol Immunotoxicol. 2009;31(4):550-5. Doi: 10.3109/08923970902814129. Chinese Herbal Medicinal Ingredients Inhibit Secretion Of Il-6, Il-8, E-Selectin And Txb2 In Lps-Induced Rat Intestinal Microvascula...

Yang J, Yu Hm, Zhou Xd, Kolosov Vp, Perelman Jm. Mol Immunol. 2013 Jan;53(1-2):161-71. Doi: 10.1016/J.Molimm.2012.06.015. Epub 2012 Aug 30. Study On Trpv1-Mediated Mechanism For The Hypersecretion Of Mucus In Respiratory Inflammation.

Li Q, Zhou X, Kolosov Vp, Perelman Jm. Cell Biochem Biophys. 2012 Apr;62(3):421-31. Doi: 10.1007/S12013-011-9324-Z. The Expression And Pharmacological Characterization Of Nicotinic Acetylcholine Receptor Subunits In Hbe16 Airway Epithelial Cells.

Yu J, Lu Y, Li Y, Xiao L, Xing Y, Li Y, Wu L. J Pharm Pharmacol. 2015 Sep;67(9):1240-50. Doi: 10.1111/Jphp.12415. Epub 2015 Apr 16. Role Of S100A1 In Hypoxia-Induced Inflammatory Response In Cardiomyocytes Via Tlr4/Ros/Nf-??b Pathway.

Kong J, Kong L, Kong J, Ke S, Gao J, Ding X, Zheng L, Sun H, Sun W. J Transl Med. 2012 Nov 21;10:230. Doi: 10.1186/1479-5876-10-230. After Insufficient Radiofrequency Ablation, Tumor-Associated Endothelial Cells Exhibit Enhanced Angiogenesis And P...

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Yu Zm, Liu Zh, Chen J, Zeng Q. Chin J Integr Med. 2014 Dec;20(12):934-43. Doi: 10.1007/S11655-014-1863-X. Epub 2014 Nov 27. Anti-Inflammatory Effect Of Qingwen Baidu Decoction (????????????

Liu W, Shan Lp, Dong Xs, Liu Xw, Ma T, Liu Z. World J Gastroenterol. 2013 Jan 28;19(4):492-502. Doi: 10.3748/Wjg.V19.I4.492. Combined Early Fluid Resuscitation And Hydrogen Inhalation Attenuates Lung And Intestine Injury.

Yu Q, Yuan L, Deng J, Yang Q. Front Cell Infect Microbiol. 2015 Mar 25;5:26. Doi: 10.3389/Fcimb.2015.00026. Ecollection 2015. Lactobacillus Protects The Integrity Of Intestinal Epithelial Barrier Damaged By Pathogenic Bacteria.

Zhang Wg, He L, Shi Xm, Wu Ss, Zhang B, Mei L, Xu Yj, Zhang Zx, Zhao Jp, Zhang Hl. Respir Res. 2014 Mar 25;15:33. Doi: 10.1186/1465-9921-15-33. Regulation Of Transplanted Mesenchymal Stem Cells By The Lung Progenitor Niche In Rats With Chronic Obs...

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8 Customer Q&As for Human IL-8/Interleukin-8/CXCL8 ELISA Kit PicoKine™

Question

Would EK0413 be suitable for analyzing tissue samples? If yes, what protocol for preparing tissue samples is recommended as a starting point for optimization?

Verified customer

Asked: 2020-11-03

Answer

Our lab hasn't worked on tissue homogenate samples using the Human IL-8 ELISA Kit PicoKine™ (EK0413). Endogenous biotin in tissue homogenates might introduce false positive signals. We recommend the customer to run a pilot test as described below to confirm the effect of endogenous biotin first. Add tissue homogenates into the wells and then add ABC abd TMB without adding any biotinylated detection antibody to see if any signal will be observed. If no signal is produced, the customer can work on the tissue sample by using the kit. Please see the following suggested protocol. Procedure for Lysis of Tissues Note: Pre-chill an appropriate volume of IP Lysis Buffer at 4 ˚C. If desired, add protease inhibitor and phosphatase inhibitor to the lysis buffer immediately before use. 1.Place the fresh tissue into chilled PBS and rinse several times. Mince the tissue into small pieces. 2.Add ice cold IP lysis buffer(Cat#AR0107) to the tissue at 10:1(v/w). (i.e., Add 1ml lysis buffer to 0.1g tissue sample.) Use a larger volume of reagent if lysis is insufficient. Use a smaller volume of reagent if a more concentrated protein extract is required. 3.Homogenize for several minutes with a glass homogenizer at high speed until no tissue chunks remain. 4.Incubate on ice for 10 minutes. 5.Centrifuge at ~13000 x g for 10 minutes at 4°C. 6.Transfer supernatant to a new tube for further analysis.

Boster Scientific Support

Answered: 2020-11-04

Question

Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2020-05-03

Answer

A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can effect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2020-05-03

Question

Q: is it okay to use citrate plasma as samples in Human CXCL8 Picokine® ELISA Kit (Catalog # EK0413)?

Verified Customer

Verified customer

Asked: 2020-04-18

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of CXCL8 causing degradation of its protein structure. CXCL8 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the CXCL8 ELISA, treating samples with various anticoagulants and decided that heparin, EDTA or citrate can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2020-04-18

Question

Can EK0413 be used to run the Enzyme-Linked Immunosorbent Assay (ELISA) test for Human salivary obtained from gum?

Verified customer

Asked: 2019-10-20

Answer

Our lab hasn't tested the Human IL-8 ELISA Kit PicoKine EK0413 on human salivary. It is uncertain if the kits are able to work on human salivary. We suggest to run a pilot test if you want to try. In theory, if the expression level of the target protein in the salivary falls in the assay range of the kit, the kit should be able to detect the protein in the sample.

Boster Scientific Support

Answered: 2019-10-21

Question

Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification CXCL8 using the EK0413 Human CXCL8 Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2019-08-31

Answer

A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples to prevent protein degradation.

Boster Scientific Support

Answered: 2019-08-31

Question

I used your product EK0413 for urine samples, should it be diluted with the standard diluent human serum? Or standard diluent buffer?

Verified Customer

Verified customer

Asked: 2018-02-01

Answer

The EK0413 kit is suitable to use with all human body fluids, however urine samples should be diluted in standard buffer containing PBS BSA. We suggest to start the dilution at 1:1(50 uL of samples and 50 uL of diluent).

Boster Scientific Support

Answered: 2018-02-01

Question

Q: how do I analyze ELISA data? I have performed CXCL8 level in serum.

Verified Customer

Verified customer

Asked: 2018-01-08

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2018-01-08

Question

I am a researcher from Istanbul, Y?ld?z Technical University. I want to run the Enzyme-Linked Immunosorbent Assay (ELISA) test for Human salivary obtained from the gum. Currently, I have BOSTER Human IL-8 PicoKine

Verified Customer

Verified customer

Asked: 2018-01-04

Answer

Our lab hasn't tested EK0413 on human salivary. We are uncertain if the kits are able to work on human salivary. Please suggest the custome run a pilot test if he wants to try. In theory, if the expression level of the target protein in the salivary falls in the assay range of the kit, the kit is able to detect the protein in the sample.

Boster Scientific Support

Answered: 2018-01-04

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