Human IL-8/Interleukin-8/CXCL8 ELISA Kit PicoKine®

CXCL8/IL-8 ELISA kit for Human

Human IL-8/Interleukin-8/CXCL8 ELISA Kit PicoKine™ (96 Tests). Quantitate Human CXCL8 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 1pg/ml. Cited in 10 publication(s).

Product Info Summary

SKU: EK0413
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

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Product Name

Human IL-8/Interleukin-8/CXCL8 ELISA Kit PicoKine®

View all CXCL8/IL-8 ELISA kits

SKU/Catalog Number

EK0413

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human IL-8/Interleukin-8/CXCL8 ELISA Kit PicoKine™ (96 Tests). Quantitate Human CXCL8 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 1pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human IL-8/Interleukin-8/CXCL8 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0413)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: S28-S99

Sensitivity

<1 pg/ml

Assay Range

15.6 pg/ml - 1,000 pg/ml

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0413 is reactive to CXCL8 in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Application Guarantee

EK0413 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of CXCL8/IL-8

Interleukin-8, also called neutrophil-activating peptide-1 or SCYB8, is a tissue-derived peptide secreted by several types of cells in response to inflammatory stimuli. Monocyte-derived neutrophil chemotactic factor (MDNCF/IL-8, suggested gene symbol IL8) is a cytokine that chemoattracts and activates neutrophils. IL-8 is produced and released from human adipose tissue and from isolated adipocytes in vitro, which may indicate that IL-8 from adipose tissue could be involved in some of the obesity-related complications. The MDNCF/IL-8 gene is placed on the human gene map at position 4q12-q21. This is the same location where at least three other members (platelet factor 4, melanoma growth stimulatory activity, and interferon-gamma induced factor) of the platelet factor 4 gene superfamily reside. Human IL-8 consists of 99 amino acids in precursor form and 79 amino acids in mature form.

Kit Components

Catalog Number Description Quantity
EK0413-CAP Anti-Human CXCL8 Pre-coated 96-well strip microplate 1
EK0413-ST Human CXCL8 Standard 2 vials, 10 ng/tube
EK0413-DA Human CXCL8 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015.631.262.51252505001000
O.D.0.0000.0820.1490.2810.5560.9361.5942.071

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect CXCL8/IL-8, Dilution ratio of 1:1, concentration in serum is target in serum is below detection limit..

Some articles we found to cite concentrations of CXCL8/IL-8 in samples: 15529589 (Pubmed IDs).

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human IL-8/Interleukin-8/CXCL8 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)16662341663230
Standard deviation0.722.8314.270.83.8417.48
CV (%)4.5%4.3%6.1%5%6.1%7.6%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of CXCL8/IL-8 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 116161517160.74.3%
Sample 266677274693.344.8%
Sample 323424925522123913.255.5%
*number of samples for each test n=16.

Gene/Protein Information For CXCL8 (Source: Uniprot.Org, NCBI)

Uniprot ID

P10145

Gene ID

3576

Gene Name

CXCL8

Full Name

Interleukin-8

Weight

11.098kDa

Superfamily

intercrine alpha (chemokine CxC) family

Alternative Names

3-10C; AMCF-I; C-X-C motif chemokine 8; CXCL8; CXCL8SCYB8; Emoctakin; GCP1; GCP-1TSG-1; IL8; IL-8; interleukin 8; K60; LAI; LECT; LUCT; LYNAP; MDNCF; MDNCFb-ENAP; member 8; MONAP; MONAPGCP1; NAF; NAP1; NAP-1NAP1; NCF; Neutrophil-activating protein 1; Protein 3-10C; T cell chemotactic factor; T-cell chemotactic factor; TCF; TSG1 CXCL8 GCP-1, GCP1, IL8, LECT, LUCT, LYNAP, MDNCF, MONAP, NAF, NAP-1, NAP1, SCYB8 C-X-C motif chemokine ligand 8 interleukin-8|T-cell chemotactic factor|alveolar macrophage chemotactic factor I|beta endothelial cell-derived neutrophil activating peptide|beta-thromboglobulin-like protein|chemokine (C-X-C motif) ligand 8|emoctakin|granulocyte chemotactic protein 1|interleukin 8|lung giant cell carcinoma-derived chemotactic protein|lymphocyte derived neutrophil activating peptide|monocyte-derived neutrophil chemotactic factor|monocyte-derived neutrophil-activating peptide|neutrophil-activating peptide 1|small inducible cytokine subfamily B, member 8|tumor necrosis factor-induced gene 1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CXCL8, check out the CXCL8 Infographic

CXCL8 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CXCL8: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0413 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

The effect of the surgical microscope on the outcome of root scaling

Identification of genes involved in enterovirus 71 infected SK-N-SH cells

Inflammatory response of macrophages cultured with Helicobacter pylori strains was regulated by miR-155

Exhaled Hydrogen Sulfide Predicts Airway Inflammation Phenotype in COPD

Evaluation of optimized continuous venovenous hemodiafiltration therapy efficiency in severe burn patients with sepsis

Inflammatory responses induced by Helicobacter pylori on the carcinogenesis of gastric epithelial GES‑1 cells

Mechanisms of the lipopolysaccharide‑induced inflammatory response in alveolar epithelial cell/macrophage co‑culture

Differential clinical benefits of continuous blood purification treatment in critically ill patients with variable APACHE II scores

Randomized, double-blind, placebo-controlled superiority trial of the Yiqigubiao pill for the treatment of patients with chronic obstructive pulmonary disease at a stable stage

Characteristics of CD4+CD25+Foxp3+ regulatory T cells in patients with multiple organ dysfunction syndrome

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8 Customer Q&As for Human IL-8/Interleukin-8/CXCL8 ELISA Kit PicoKine®

Question

Would EK0413 be suitable for analyzing tissue samples? If yes, what protocol for preparing tissue samples is recommended as a starting point for optimization?

Verified customer

Asked: 2020-11-03

Answer

Our lab hasn't worked on tissue homogenate samples using the Human IL-8 ELISA Kit PicoKine™ (EK0413). Endogenous biotin in tissue homogenates might introduce false positive signals. We recommend the customer to run a pilot test as described below to confirm the effect of endogenous biotin first. Add tissue homogenates into the wells and then add ABC abd TMB without adding any biotinylated detection antibody to see if any signal will be observed. If no signal is produced, the customer can work on the tissue sample by using the kit. Please see the following suggested protocol. Procedure for Lysis of Tissues Note: Pre-chill an appropriate volume of IP Lysis Buffer at 4 ˚C. If desired, add protease inhibitor and phosphatase inhibitor to the lysis buffer immediately before use. 1.Place the fresh tissue into chilled PBS and rinse several times. Mince the tissue into small pieces. 2.Add ice cold IP lysis buffer(Cat#AR0107) to the tissue at 10:1(v/w). (i.e., Add 1ml lysis buffer to 0.1g tissue sample.) Use a larger volume of reagent if lysis is insufficient. Use a smaller volume of reagent if a more concentrated protein extract is required. 3.Homogenize for several minutes with a glass homogenizer at high speed until no tissue chunks remain. 4.Incubate on ice for 10 minutes. 5.Centrifuge at ~13000 x g for 10 minutes at 4°C. 6.Transfer supernatant to a new tube for further analysis.

Boster Scientific Support

Answered: 2020-11-04

Question

Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2020-05-03

Answer

A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can effect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2020-05-03

Question

Q: is it okay to use citrate plasma as samples in Human CXCL8 Picokine® ELISA Kit (Catalog # EK0413)?

Verified Customer

Verified customer

Asked: 2020-04-18

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of CXCL8 causing degradation of its protein structure. CXCL8 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the CXCL8 ELISA, treating samples with various anticoagulants and decided that heparin, EDTA or citrate can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2020-04-18

Question

Can EK0413 be used to run the Enzyme-Linked Immunosorbent Assay (ELISA) test for Human salivary obtained from gum?

Verified customer

Asked: 2019-10-20

Answer

Our lab hasn't tested the Human IL-8 ELISA Kit PicoKine EK0413 on human salivary. It is uncertain if the kits are able to work on human salivary. We suggest to run a pilot test if you want to try. In theory, if the expression level of the target protein in the salivary falls in the assay range of the kit, the kit should be able to detect the protein in the sample.

Boster Scientific Support

Answered: 2019-10-21

Question

Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification CXCL8 using the EK0413 Human CXCL8 Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2019-08-31

Answer

A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples to prevent protein degradation.

Boster Scientific Support

Answered: 2019-08-31

Question

I used your product EK0413 for urine samples, should it be diluted with the standard diluent human serum? Or standard diluent buffer?

Verified Customer

Verified customer

Asked: 2018-02-01

Answer

The EK0413 kit is suitable to use with all human body fluids, however urine samples should be diluted in standard buffer containing PBS BSA. We suggest to start the dilution at 1:1(50 uL of samples and 50 uL of diluent).

Boster Scientific Support

Answered: 2018-02-01

Question

Q: how do I analyze ELISA data? I have performed CXCL8 level in serum.

Verified Customer

Verified customer

Asked: 2018-01-08

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2018-01-08

Question

I am a researcher from Istanbul, Y?ld?z Technical University. I want to run the Enzyme-Linked Immunosorbent Assay (ELISA) test for Human salivary obtained from the gum. Currently, I have BOSTER Human IL-8 PicoKine

Verified Customer

Verified customer

Asked: 2018-01-04

Answer

Our lab hasn't tested EK0413 on human salivary. We are uncertain if the kits are able to work on human salivary. Please suggest the custome run a pilot test if he wants to try. In theory, if the expression level of the target protein in the salivary falls in the assay range of the kit, the kit is able to detect the protein in the sample.

Boster Scientific Support

Answered: 2018-01-04

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