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Product Info Summary
| SKU: | EK0413 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA, citrate). |
Product info
Product Name
Human IL-8/Interleukin-8/CXCL8 ELISA Kit PicoKine®
SKU/Catalog Number
EK0413
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human IL-8/Interleukin-8/CXCL8 ELISA Kit PicoKine® (96 Tests). Quantitate Human CXCL8 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human IL-8/Interleukin-8/CXCL8 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0413)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: E.coli; Immunogen sequence: S28-S99
Capture/Detection Antibodies
The capture antibody is monoclonal antibody from mouse and the detection antibody is polyclonal antibody from goat.
Sensitivity
<1 pg/ml
Assay Range
15.6 pg/ml - 1,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0413 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0413 is reactive to CXCL8 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
Application Guarantee
EK0413 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of CXCL8
Interleukin-8, also called neutrophil-activating peptide-1 or SCYB8, is a tissue-derived peptide secreted by several types of cells in response to inflammatory stimuli. Monocyte-derived neutrophil chemotactic factor (MDNCF/IL-8, suggested gene symbol IL8) is a cytokine that chemoattracts and activates neutrophils. IL-8 is produced and released from human adipose tissue and from isolated adipocytes in vitro, which may indicate that IL-8 from adipose tissue could be involved in some of the obesity-related complications. The MDNCF/IL-8 gene is placed on the human gene map at position 4q12-q21. This is the same location where at least three other members (platelet factor 4, melanoma growth stimulatory activity, and interferon-gamma induced factor) of the platelet factor 4 gene superfamily reside. Human IL-8 consists of 99 amino acids in precursor form and 79 amino acids in mature form.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0413-CAP | Anti-Human CXCL8 Pre-coated 96-well strip microplate | 1 |
| EK0413-ST | Human CXCL8 Standard | 2 vials, 10 ng/tube |
| EK0413-DA | Human CXCL8 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.022 | 0.066 | 0.106 | 0.199 | 0.362 | 0.681 | 1.25 | 2.095 |
Data Example Images
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Human IL-8 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect CXCL8, Dilution ratio of 1:1, concentration in serum and plasma is less than the lowest standard, 15.6 pg/ml..
Some articles we found to cite concentrations of CXCL8 in samples: 15529589 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human IL-8/Interleukin-8/CXCL8 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 16 | 66 | 234 | 16 | 63 | 230 |
| Standard deviation | 0.72 | 2.83 | 14.27 | 0.8 | 3.84 | 17.48 |
| CV (%) | 4.5% | 4.3% | 6.1% | 5% | 6.1% | 7.6% |
Specific Publications For Human IL-8/Interleukin-8/CXCL8 ELISA Kit PicoKine® (EK0413)
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Customer Reviews
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Customer Q&As
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8 Customer Q&As for Human IL-8/Interleukin-8/CXCL8 ELISA Kit PicoKine®
Question
Would EK0413 be suitable for analyzing tissue samples? If yes, what protocol for preparing tissue samples is recommended as a starting point for optimization?
Verified customer
Asked: 2020-11-03
Answer
Our lab hasn't worked on tissue homogenate samples using the Human IL-8 ELISA Kit PicoKine™ (EK0413). Endogenous biotin in tissue homogenates might introduce false positive signals. We recommend the customer to run a pilot test as described below to confirm the effect of endogenous biotin first. Add tissue homogenates into the wells and then add ABC abd TMB without adding any biotinylated detection antibody to see if any signal will be observed. If no signal is produced, the customer can work on the tissue sample by using the kit. Please see the following suggested protocol. Procedure for Lysis of Tissues Note: Pre-chill an appropriate volume of IP Lysis Buffer at 4 ˚C. If desired, add protease inhibitor and phosphatase inhibitor to the lysis buffer immediately before use. 1.Place the fresh tissue into chilled PBS and rinse several times. Mince the tissue into small pieces. 2.Add ice cold IP lysis buffer(Cat#AR0107) to the tissue at 10:1(v/w). (i.e., Add 1ml lysis buffer to 0.1g tissue sample.) Use a larger volume of reagent if lysis is insufficient. Use a smaller volume of reagent if a more concentrated protein extract is required. 3.Homogenize for several minutes with a glass homogenizer at high speed until no tissue chunks remain. 4.Incubate on ice for 10 minutes. 5.Centrifuge at ~13000 x g for 10 minutes at 4°C. 6.Transfer supernatant to a new tube for further analysis.
Boster Scientific Support
Answered: 2020-11-04
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2020-05-03
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can effect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2020-05-03
Question
Q: is it okay to use citrate plasma as samples in Human CXCL8 Picokine® ELISA Kit (Catalog # EK0413)?
Verified Customer
Verified customer
Asked: 2020-04-18
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of CXCL8 causing degradation of its protein structure. CXCL8 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the CXCL8 ELISA, treating samples with various anticoagulants and decided that heparin, EDTA or citrate can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2020-04-18
Question
Can EK0413 be used to run the Enzyme-Linked Immunosorbent Assay (ELISA) test for Human salivary obtained from gum?
Verified customer
Asked: 2019-10-20
Answer
Our lab hasn't tested the Human IL-8 ELISA Kit PicoKine EK0413 on human salivary. It is uncertain if the kits are able to work on human salivary. We suggest to run a pilot test if you want to try. In theory, if the expression level of the target protein in the salivary falls in the assay range of the kit, the kit should be able to detect the protein in the sample.
Boster Scientific Support
Answered: 2019-10-21
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification CXCL8 using the EK0413 Human CXCL8 Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2019-08-31
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples to prevent protein degradation.
Boster Scientific Support
Answered: 2019-08-31
Question
I used your product EK0413 for urine samples, should it be diluted with the standard diluent human serum? Or standard diluent buffer?
Verified Customer
Verified customer
Asked: 2018-02-01
Answer
The EK0413 kit is suitable to use with all human body fluids, however urine samples should be diluted in standard buffer containing PBS BSA. We suggest to start the dilution at 1:1(50 uL of samples and 50 uL of diluent).
Boster Scientific Support
Answered: 2018-02-01
Question
Q: how do I analyze ELISA data? I have performed CXCL8 level in serum.
Verified Customer
Verified customer
Asked: 2018-01-08
Answer
A: please read this article on ELISA data analysis. bosterbio.com/elisa-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/elisa-data-analysis-online
Boster Scientific Support
Answered: 2018-01-08
Question
I am a researcher from Istanbul, Y?ld?z Technical University. I want to run the Enzyme-Linked Immunosorbent Assay (ELISA) test for Human salivary obtained from the gum. Currently, I have BOSTER Human IL-8 PicoKine
Verified Customer
Verified customer
Asked: 2018-01-04
Answer
Our lab hasn't tested EK0413 on human salivary. We are uncertain if the kits are able to work on human salivary. Please suggest the custome run a pilot test if he wants to try. In theory, if the expression level of the target protein in the salivary falls in the assay range of the kit, the kit is able to detect the protein in the sample.
Boster Scientific Support
Answered: 2018-01-04
