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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).|
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Human Kallikrein-6 / KLK6 ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human Kallikrein-6 / KLK6 ELISA Kit PicoKine™ (96 Tests). Quantitate Human KLK6 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 3pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Kallikrein-6 / KLK6 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0818)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: L22-K244
78 pg/ml - 5,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK0818 is reactive to KLK6 in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
EK0818 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Kallikrein 6/Neurosin
KLK6 (Kallikrein-related peptidase 6), also called KALLIKREIN 6, NEUROSIN, PROTEASE M, ZYME or PRSS9, is a protein that in humans is encoded by the KLK6 gene. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. The encoded enzyme is regulated by steroid hormones. Northern blot analysis revealed that the PRSS9 mRNA was expressed in several primary tumors and cell lines from mammary, prostate, and ovarian cancers, but was not detected in any metastases of these cancers. The KLK6 gene is mapped on 19q13.41. In tissue culture, the enzyme has been found to generate amyloidogenic fragments from the amyloid precursor protein, suggesting a potential for involvement in Alzheimer's disease. Upon cellular stress, neurosin was released from mitochondria to the cytosol, which resulted in the increase of degraded alpha-synuclein species. Neurosin may play a significant role in physiologic alpha-synuclein degradation and also in the pathogenesis of synucleinopathies.
|EK0818-CAP||Anti-Human KLK6 Pre-coated 96-well strip microplate||1|
|EK0818-ST||Human KLK6 Standard||2 vials, 10 ng/tube|
|EK0818-DA||Human KLK6 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human Kallikrein-6 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Kallikrein-6 / KLK6 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of Kallikrein 6/Neurosin in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For KLK6 (Source: Uniprot.Org, NCBI)
peptidase S1 family
Bssp; EC 3.4.21; EC 3.4.21.-; hK6; kallikrein 6 (neurosin, zyme); Kallikrein 6; kallikrein-6; kallikrein-related peptidase 6; KLK6; Klk7; Neurosin; Protease M; protease, serine, 18; PRSS18; PRSS9; PRSS9MGC9355; Serine protease 18; Serine protease 9; SP59; Zyme KLK6 Bssp, Klk7, PRSS18, PRSS9, SP59, hK6 kallikrein related peptidase 6 kallikrein-6|neurosin|protease M|serine protease 18|serine protease 9|zyme*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on KLK6, check out the KLK6 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for KLK6: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
EK0818 has been cited in 4 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Molecular Pathways Associated with Kallikrein 6 Overexpression in Colorectal Cancer
Kallikrein 6 protease advances colon tumorigenesis via induction of the high mobility group A2 protein
Lei Yuan,Dongdong Zou,Xia Yang et al.Proteomic and Functional Analyses Reveal That Kallikrein 6 Enhances Communicating Hydrocephalus by Injuring Neuronal Synapses,24 May 2021,PREPRINT (Version 1) available at Research Square [https://doi.org/10.21203/rs.3.rs-403838/v1]
EK0818 usage in article: APP:ELISA, SAMPLE:CSF AND BLOOD, DILUTION:NA
Sells E, Pandey R, Chen H, Skovan BA, Cui H, Ignatenko NA. Neoplasia. 2017 May;19(5):396-411. doi: 10.1016/j.neo.2017.02.003. Epub 2017 Apr 18. Specific microRNA
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