Human LBP ELISA Kit PicoKine™

Human LBP ELISA Kit PicoKine™ (96 Tests). Quantitate Human LBP in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml. Cited in 3 publication(s).

Product Info Summary

SKU: EK1271
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Human LBP ELISA Kit PicoKine™

See all LBP products

SKU/Catalog Number

EK1271

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human LBP ELISA Kit PicoKine™ (96 Tests). Quantitate Human LBP in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human LBP ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1271)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: A26-V481

Sensitivity

<50 pg/ml

Assay Range

0.78 ng/ml - 50 ng/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1271 is reactive to LBP in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK1271 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK1271-CAP Anti-Human LBP Pre-coated 96-well strip microplate 1
EK1271-ST Human LBP Standard 2 vials, 50 ng/tube
EK1271-DA Human LBP Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Validation Standard Curve O.D. At 450nm

Concentration (ng/ml)00.781.563.126.2512.52550
O.D.0.0220.0530.0880.1480.3420.7791.3011.985

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect LBP, Dilution ratio of 1:1000, concentration in serum is 1000-6000ng/ml.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human LBP ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)14854533235621518457521374
Standard deviation66.82271.981507.9685297.371432.05
CV (%)4.5%6%6.4%5.6%6.5%6.7%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of LBP in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 11485156116301640157962.213.9%
Sample 245335189443847034715289.256.1%
Sample 32356222985219072203522622683.953%
*number of samples for each test n=16.

Gene/Protein Information For LBP (Source: Uniprot.Org, NCBI)

Uniprot ID

P18428

Gene ID

3929

Gene Name

LBP

Full Name

Lipopolysaccharide-binding protein

Weight

53.384kDa

Superfamily

BPI/LBP/Plunc superfamily

Alternative Names

LBP; lipopolysaccharide binding protein; lipopolysaccharide-binding protein; LPS-binding protein; MGC22233 LBP BPIFD2 lipopolysaccharide binding protein lipopolysaccharide-binding protein|BPI fold containing family D, member 2|LPS-binding protein

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on LBP, check out the LBP Infographic

LBP infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for LBP: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK1271 has been cited in 3 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Xiaoling Zhou,Xingwei Xiang,Yufang Zhou,Tianyi Zhou,Shanggui Deng,Bin Zheng,Pingan Zheng,Protective effects of Antarctic krill oil in dextran sulfate sodium-induced ulcerative colitis mice,Journal of Functional Foods,Volume 79,2021,104394,ISSN 1756-4646,h
Species: Mouse
EK1271 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

Serrano-Villar S,Talavera-Rodríguez A,Gosalbes MJ,Madrid N,Pérez-Molina JA,Elliott RJ,Navia B,Lanza VF,Vallejo A,Osman M,Dronda F,Budree S,Zamora J,Gutiérrez C,Manzano M,Vivancos MJ,Ron R,Martínez-Sanz J,Herrera S,Ansa U,Moya A,Moreno S. Fecal microbiota
Species: Human
EK1271 usage in article: APP:ELISA, SAMPLE:PLASMA, DILUTION:NA

Reduction Impairs the Antibacterial Activity but Benefits the LPS Neutralization Ability of Human Enteric Defensin 5

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Find answers in Q&As, reviews.

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9 Customer Q&As for Human LBP ELISA Kit PicoKine™

Question

In the assay protocol step 5-6 for EK1271, is there really no washing step between the standard incubation and biotynlated antibody step?

Verified customer

Asked: 2021-01-25

Answer

For the Human LBP ELISA Kit PicoKine™ (EK1271), there is no wash step between the standard incubation and biotinylated antibody step. Our lab technicians found there wasn't any significant difference in result values between washing and without washing the plate after incubating samples/standard and before addition of the detection antibody. It is to make the protocol easy and fast.

Boster Scientific Support

Answered: 2021-01-25

Question

Q: how to thaw whole blood sample for LBP ELISA after freezing?

Verified Customer

Verified customer

Asked: 2020-06-17

Answer

A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test LBP for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.

Boster Scientific Support

Answered: 2020-06-17

Question

Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2020-05-13

Answer

A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can have an affect on immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2020-05-13

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2019-12-04

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2019-12-04

Question

Do you have suggestions in handling the blood samples for use with EK1271? Can it be stored at room temperature for a day?

Verified customer

Asked: 2019-11-22

Answer

Generally, the collected samples for ELISA analysis can be kept for different periods: 48 hours (2-8°C), 1 month (-20°C) or 6 months (-70°C), but it would be best if you could store them at (2-8°C) for 1 day after collecting the samples with heparin tubes.

Boster Scientific Support

Answered: 2019-11-22

Question

Q: is it okay to use citrate plasma as samples in Human LBP Picokine® ELISA Kit (Catalog # EK1271)?

Verified Customer

Verified customer

Asked: 2018-12-25

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of LBP causing degradation of its protein structure. LBP may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the LBP ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2018-12-25

Question

Q: Can LBP ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

G. King

Verified customer

Asked: 2018-05-31

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2018-05-31

Question

Q: we need your recommendation regarding the dilution ratio of serum samples for detection of LBP in Human plasma? I am trying to measure a few parameters and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.

Z. Garcia

Verified customer

Asked: 2018-04-17

Answer

A: without knowing the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the LBP ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2018-04-17

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

Verified Customer

Verified customer

Asked: 2018-04-13

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2018-04-13

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