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Product Info Summary
| SKU: | EK1271 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human LBP ELISA Kit PicoKine®
SKU/Catalog Number
EK1271
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human LBP ELISA Kit PicoKine® (96 Tests). Quantitate Human LBP in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human LBP ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1271)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: A26-V481
Capture/Detection Antibodies
The capture antibody is polyclonal antibody from goat and the detection antibody is polyclonal antibody from goat.
Sensitivity
<50 pg/ml
Assay Range
0.78 ng/ml - 50 ng/ml
Standard Dilution Instructions
See datasheet of EK1271 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1271 is reactive to LBP in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK1271 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of LBP
Lipopolysaccharide binding protein is a protein that in humans is encoded by the LBP gene. This gene is mapped to 20q11.23. LBP is a soluble acute-phase protein that binds to bacterial lipopolysaccharide (or LPS) to elicit immune responses by presenting the LPS to important cell surface pattern recognition receptors called CD14 and TLR4. It is present in the cerebrospinal fluid of patients with pneumococcal meningitis. The protein encoded by this gene is involved in the acute-phase immunologic response to gram-negative bacterial infections. LBP is made in the liver during the acute phase of infections and is thought to function as a carrier for LPS and to help control LPS-dependent monocyte responses.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1271-CAP | Anti-Human LBP Pre-coated 96-well strip microplate | 1 |
| EK1271-ST | Human LBP Standard | 2 vials, 50 ng/tube |
| EK1271-DA | Human LBP Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (ng/ml) | 0 | 0.78 | 1.56 | 3.12 | 6.25 | 12.5 | 25 | 50 |
| O.D. | 0.021 | 0.078 | 0.124 | 0.227 | 0.398 | 0.712 | 1.242 | 1.802 |
Data Example Images
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Human LBP PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect LBP, Dilution ratio of 1:1000, concentration in serum and plasma is 1000-6000 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human LBP ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 1485 | 4533 | 23562 | 1518 | 4575 | 21374 |
| Standard deviation | 66.82 | 271.98 | 1507.96 | 85 | 297.37 | 1432.05 |
| CV (%) | 4.5% | 6% | 6.4% | 5.6% | 6.5% | 6.7% |
Specific Publications For Human LBP ELISA Kit PicoKine® (EK1271)
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Customer Reviews
0 Reviews For Human LBP ELISA Kit PicoKine®
Customer Q&As
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14 Customer Q&As for Human LBP ELISA Kit PicoKine®
Question
Are there any internal threshold to determine whether the particular lot passes QC for EK1271?
Verified customer
Asked: 2022-05-20
Answer
For the Human LBP ELISA Kit PicoKine® (EK1271), our lab evaluates our kits in terms of precision, reproducibility, linearity, recovery and specificity. Precision: CV of Intra Assay Consistency & Inter Assay Consistency ≤ 10%. Reproducibility: Samples with differing target concentrations were assayed using different lots of the kits to ensure reproducibility. CV%≤ 10% Linearity: Values between 80–120% are acceptable. Recovery: Our acceptance range for the recovery is 80–120%. Specificity: Our Kits are tested against related molecules to ensure no cross-reactivity or interference.
Boster Scientific Support
Answered: 2022-05-20
Question
Is the general dilution factor of human serum between 1:1000 to 1:10000 when using EK1271?
Verified customer
Asked: 2022-05-20
Answer
Our lab used 1:2000, 1:4000 and 1:8000. We suggest to optimize diluiton ratios for the specific samples on the basis of 1:2000 when using the Human LBP ELISA Kit PicoKine® (EK1271).
Boster Scientific Support
Answered: 2022-05-20
Question
Do you know any possible reason that could have contributed to the high CV% when using EK1271?
Verified customer
Asked: 2022-05-20
Answer
Some operational error and inappropriate dilutions might contributed to the high CV% when using the Human LBP ELISA Kit PicoKine® (EK1271).
Boster Scientific Support
Answered: 2022-05-20
Question
Any possible optimization steps for EK1271?
Verified customer
Asked: 2022-05-05
Answer
We suggest to strictly adhere to the protocol listed on the provided datasheet of the Human LBP ELISA Kit PicoKine® (EK1271). Please refer to the provided COA and use dilution of 1:2000 and optimize diluiton ratios for the specific samples.
Boster Scientific Support
Answered: 2022-05-05
Question
Was EK1271 validated for cell culture supernatant using media containing FBS?
Verified customer
Asked: 2022-03-09
Answer
For Human LBP ELISA Kit PicoKine® (EK1271), FBS in the cell culture media will not interfere with the assay.
Boster Scientific Support
Answered: 2022-03-11
Question
In the assay protocol step 5-6 for EK1271, is there really no washing step between the standard incubation and biotynlated antibody step?
Verified customer
Asked: 2021-01-25
Answer
For the Human LBP ELISA Kit PicoKine™ (EK1271), there is no wash step between the standard incubation and biotinylated antibody step. Our lab technicians found there wasn't any significant difference in result values between washing and without washing the plate after incubating samples/standard and before addition of the detection antibody. It is to make the protocol easy and fast.
Boster Scientific Support
Answered: 2021-01-25
Question
Q: how to thaw whole blood sample for LBP ELISA after freezing?
Verified Customer
Verified customer
Asked: 2020-06-17
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test LBP for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2020-06-17
Question
Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2020-05-13
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can have an affect on immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2020-05-13
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2019-12-04
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2019-12-04
Question
Do you have suggestions in handling the blood samples for use with EK1271, EK1410 and EK0694? Can it be stored at room temperature for a day?
Verified customer
Asked: 2019-11-22
Answer
Generally, the collected samples for ELISA analysis can be kept for different periods: 48 hours (2-8°C), 1 month (-20°C) or 6 months (-70°C), but it would be best if you could store them at (2-8°C) for 1 day after collecting the samples with heparin tubes.
Boster Scientific Support
Answered: 2019-11-22
Question
Q: is it okay to use citrate plasma as samples in Human LBP Picokine® ELISA Kit (Catalog # EK1271)?
Verified Customer
Verified customer
Asked: 2018-12-25
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of LBP causing degradation of its protein structure. LBP may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the LBP ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2018-12-25
Question
Q: Can LBP ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
G. King
Verified customer
Asked: 2018-05-31
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2018-05-31
Question
Q: we need your recommendation regarding the dilution ratio of serum samples for detection of LBP in Human plasma? I am trying to measure a few parameters and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.
Z. Garcia
Verified customer
Asked: 2018-04-17
Answer
A: without knowing the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the LBP ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/elisa-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2018-04-17
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2018-04-13
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/elisa-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/elisa-data-analysis-instructions
Boster Scientific Support
Answered: 2018-04-13
