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Human Leptin ELISA Kit PicoKine™
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Leptin. 96wells/kit, with removable strips. Cited in 26 publication(s).
Human Leptin ELISA Kit PicoKine™ Info At A Glance
| SKU: | EK0437 |
|---|---|
| Size: | 96wells/kit, with removable strips. |
| Reactivity: | Human |
| Sample Type: | cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). |
| Sample Volume: | 100μl per well |
| Sensitivity: | <10pg/ml |
| Assay Range: | 62.5pg/ml-4000pg/ml |
Overview
| Product Name | Human Leptin ELISA Kit PicoKine™
See all LEP primary antibodies, ELISA kits and proteins |
|---|---|
| SKU/Catalog Number | EK0437 |
| Storage & Handling | Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.) |
| Description | Detect Human Leptin/LEP with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Leptin/LEP tissue specificity: |
| Cite This Product | Human Leptin ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0437) |
Product Specs
| Reactivity/Species | Human |
|---|---|
| Applications | ELISA
*Our Boster Guarantee covers the use of this product in the above tested applications. **For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab. |
| Sample Type | cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). |
| Sensitivity | <10pg/ml |
| Assay Range | 62.5pg/ml-4000pg/ml |
| Immunogen | Expression system for standard: E.coli; Immunogen sequence: V22-C167 |
| Antibody Clonalities | Capture antibody|Detection antibody monoclonal antibody from mouse|polyclonal antibody from goat |
| Cross Reactivity | There is no detectable cross-reactivity with other relevant proteins. |
| Pack Size | 96wells/kit, with removable strips. |
Protein Target Info (Source: Uniprot.org)
| Uniprot Id | A4D0Y8 |
|---|---|
| Gene Name | LEP |
| Protein Name | Leptin |
| Subcellular Localization | Secreted . |
*if product is indicated to react with multiple species, protein info is based on the human gene.
Ontology
| Protein Function | May function as part of a signaling pathway that acts to regulate the size of the body fat depot. . |
|---|---|
| Background | Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. Functions as a hormone. |
Kit Components And Assay QC Data Details Of Human Leptin ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
| Catalog number | Description | Quantity |
|---|---|---|
| EK0437-CAP | 96-well plate precoated with anti-Human LEP antibody | 1 |
| EK0437-ST | lyophilized recombinant Human LEP standard | 10ng/tube |
| EK0437-DA | biotinylated anti-Human LEP antibody | 130ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 130ul |
| AR1106-1 | sample diluent buffer | 30ml |
| AR1106-2 | antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EK0437 Human Leptin ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
| Concentration(pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D. | 0.002 | 0.036 | 0.080 | 0.191 | 0.505 | 0.923 | 1.801 | 2.461 |
We measured random samples of Human Leptin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 81 | 485 | 1057 | 75 | 444 | 996 |
| Standard deviation | 6.15 | 32.49 | 54.96 | 8 | 35.52 | 69.72 |
| CV(%) | 7.6% | 6.7% | 5.2% | 8.4% | 8% | 7% |
We measured the performance consistency of Human Leptin ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 81 | 83 | 84 | 87 | 83 | 2.16 | 2.6% |
| Sample 2 | 485 | 539 | 463 | 476 | 490 | 28.93 | 5.9% |
| Sample 3 | 1057 | 1101 | 1114 | 1116 | 1097 | 23.8 | 2.1% |
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
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Total number of citations: 26
| Yang Y, Zhang X, Fu Y, Yang H. Toxicol Lett. 2014 Jun 16;227(3):172-8. Doi: 10.1016/J.Toxlet.2014.03.009. Epub 2014 Apr 4. Leptin And Il-8: Two Novel Cytokines Screened Out In Childhood Lead Exposure. | PubMed ID 24709140 |
| Hajihashemi P, Azadbakht L, Hashemipor M, Kelishadi R, Esmaillzadeh A. Mol Nutr Food Res. 2014 Jun;58(6):1301-8. Doi: 10.1002/Mnfr.201300582. Epub 2014 Jan 30. Whole-Grain Intake Favorably Affects Markers Of Systemic Inflammation In Obese Children... | PubMed ID 24478050 |
| Xie X, Yang S, Zou Y, Cheng S, Wang Y, Jiang Z, Xiao J, Wang Z, Liu Y. Brain Res. 2013 Jan 23;1491:147-55. Doi: 10.1016/J.Brainres.2012.11.007. Epub 2012 Nov 14. Influence Of The Core Circadian Gene "Clock" On Obesity And Leptin Resistance In Mice. | PubMed ID 23159716 |
| Yao Y, Li Xb, Zhao W, Zeng Yy, Shen H, Xiang H, Xiao H. Lipids Health Dis. 2010 May 19;9:49. Doi: 10.1186/1476-511X-9-49. Anti-Obesity Effect Of An Isoflavone Fatty Acid Ester On Obese Mice Induced By High Fat Diet And Its Potential Mechanism. | PubMed ID 20482839 |
| Karadag As, Ertugrul Dt, Kalkan G, Bilgili Sg, Celik Ht, Takci Z, Balahoroglu R, Calka O. Dermatology. 2013;227(2):103-8. Doi: 10.1159/000351769. Epub 2013 Sep 10. The Effect Of Acitretin Treatment On Insulin Resistance, Retinol-Binding Protein-4,... | PubMed ID 24021889 |
| Wu Cc, Weng Wl, Lai Wl, Tsai Hp, Liu Wh, Lee Mh, Tsai Yc. Evid Based Complement Alternat Med. 2015;2015:391767. Doi: 10.1155/2015/391767. Epub 2015 Feb 23. Effect Of Lactobacillus Plantarum Strain K21 On High-Fat Diet-Fed Obese Mice. | PubMed ID 25802537 |
| Rasekhi H, Karandish M, Jalali Mt, Mohammadshahi M, Zarei M, Saki A, Shahbazian H. J Diabetes Metab Disord. 2015 Jan 14;14(1):1. Doi: 10.1186/S40200-014-0127-9. Ecollection 2015. Phylloquinone Supplementation Improves Glycemic Status Independent O... | PubMed ID 25654061 |
| Liu L, Gu H, Zhao Y, An L, Yang J. Exp Ther Med. 2014 Dec;8(6):1813-1818. Epub 2014 Sep 30. Glypican 4 May Be Involved In The Adipose Tissue Redistribution In High-Fat Feeding C57Bl/6J Mice With Peroxisome Proliferators-Activated Receptor ?? Agoni... | PubMed ID 25371737 |
| ??atl?? G, An??k A, K??me T, ??alan ??g, D??ndar Bn, B??ber E, Abac?? A. J Endocrinol Invest. 2015 Aug;38(8):909-13. Doi: 10.1007/S40618-015-0277-8. Epub 2015 Apr 2. Serum Nesfatin-1 And Leptin Levels In Non-Obese Girls With Premature Thelarche. | PubMed ID 25833360 |
| Acar, S., Paketçi, A., Küme, T., Tuhan, H., Gürsoy Çalan, Ö., Demir, K.,..., & Abacı, A. (2017). Serum galectin-1 levels are positively correlated with body fat and negatively with fasting glucose in obese children. Peptides, 95, 51-56. Advance on... | PubMed ID 28728946 |
| He, S., Le, N.A., Frediani, J.K., Winterberg, P.D., Jin, R., Liverman, R.,..., & Vos, M.B. (2017). Cardiometabolic risks vary by weight status in pediatric kidney and liver transplant recipients: A cross-sectional, single-center study in the USA. ... | PubMed ID 28718192 |
| Algul S, Ozdenk C, Ozcelik O. Biol Sport. 2017 Dec;34(4):339-344. doi: 10.5114/biolsport.2017.69821. Epub 2017 Sep 20. Variations in leptin, nesfatin-1 and irisin levels induced by aerobic exercise in young trained and untrained male subjects | PubMed ID 29472736 |
| Altay C, Se?il M, Demir T, Atik T, Ak?nc? G, ?zdemir Kutbay N, Keskin Temelo?lu E, Y?ld?r?m ?im?ir I, ?z???k S, Demir L, Eren E, Tuna EB, Ayta? H, Onay H, Ak?nc? B. Diagn Interv Radiol. 2017 Nov-Dec;23(6):428-434. doi: 10.5152/dir.2017.17019. Dete... | PubMed ID 29044029 |
| Elective percutaneous coronary intervention leads to significant changes in serum resistin, leptin, and adiponectin levels regardless of periprocedural myocardial injury: an observational study | PubMed ID 27443475 |
| The effect of vitamin D administration on serum leptin and adiponectin levels in end-stage renal disease patients on hemodialysis with vitamin D deficiency: A placebo-controlled double-blind clinical trial | PubMed ID 27904547 |
| The Relationship between Serum Zonulin Level and Clinical and Laboratory Parameters of Childhood Obesity | PubMed ID 28008865 |
| Perilipin polymorphisms are risk factors for the development of obesity in adolescents? A case-control study | PubMed ID 28274232 |
| Increased concentrations of serum nesfatin-1 levels in childhood with idiopathic chronic malnutrition | PubMed ID 29714458 |
| Eicosapentaenoic acid-enriched phospholipid ameliorates insulin resistance and lipid metabolism in diet-induced-obese mice. | PubMed ID 23876229 |
| Oxidative stress and adipokine levels were significantly correlated in diabetic patients with hyperglycemic crises. | PubMed ID 30774721 |
| Leptin concentration and oxidative stress in diabetic ketoacidosis. | PubMed ID 30053313 |
| The relation of leptin and soluble leptin receptor levels with metabolic and clinical parameters in obese and healthy children. | PubMed ID 24703965 |
| Leptin levels in gingival crevicular fluid during canine retraction: in vivo comparative study | PubMed ID 31056072 |
| Investigation of adropin and leptin levels in pediatric obesity-related nonalcoholic fatty liver disease. | PubMed ID 24468600 |
| Adipokines, psoriasis, systemic inflammation, and endothelial dysfunction | PubMed ID 28762474 |
| Leptin partially mediates the association between early-life nutritional supplementation and long-term glycemic status among women in a Guatemalan longitudinal cohort | PubMed ID 32069352 |
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Domestically in USA and Canada, ships next business day if available in stock.
Note: sample size takes longer to process, average 5-7 days.
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| (888) 466-3604 |
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