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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).|
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Human Leptin ELISA Kit PicoKine™
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human Leptin ELISA Kit PicoKine™ (96 Tests). Quantitate Human LEP in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Cite This Product
Human Leptin ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0437)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: E.coli; Immunogen sequence: V22-C167
62.5 pg/ml - 4,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK0437 is reactive to LEP in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
EK0437 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
|EK0437-CAP||Anti-Human LEP Pre-coated 96-well strip microplate||1|
|EK0437-ST||Human LEP Standard||2 vials, 10 ng/tube|
|EK0437-DA||Human LEP Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human Leptin PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Leptin/OB, Dilution ratio of 1:10, concentration in serum is 300-16000pg/ml.
Some articles we found to cite concentrations of Leptin/OB in samples: 24900992 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Leptin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of Leptin/OB in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For LEP (Source: Uniprot.Org, NCBI)
FLJ94114; LEP; leptin (murine obesity homolog); leptin (obesity homolog, mouse); Leptin; OB; Obese protein; obese, mouse, homolog of; Obesity factor; OBOBS LEP LEPD, OB, OBS leptin leptin|leptin (murine obesity homolog)|leptin (obesity homolog, mouse)|obese protein|obese, mouse, homolog of|obesity factor*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on LEP, check out the LEP Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for LEP: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
EK0437 has been cited in 25 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Turan S,Sarioglu FC,Erbas IM,Cavusoglu B,Karagöz E,Şişman AR,Güney SA,Güleryüz H,Abaci A,Ozturk Y,Akay AP. Altered regional grey matter volume and appetite-related hormone levels in adolescent obesity with or without binge-eating disorder. Eat Weight Diso
EK0437 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA
Leptin partially mediates the association between early-life nutritional supplementation and long-term glycemic status among women in a Guatemalan longitudinal cohort
Adipokines, psoriasis, systemic inflammation, and endothelial dysfunction
Investigation of adropin and leptin levels in pediatric obesity-related nonalcoholic fatty liver disease.
Leptin levels in gingival crevicular fluid during canine retraction: in vivo comparative study
The relation of leptin and soluble leptin receptor levels with metabolic and clinical parameters in obese and healthy children.
Leptin concentration and oxidative stress in diabetic ketoacidosis.
Increased concentrations of serum nesfatin-1 levels in childhood with idiopathic chronic malnutrition
Perilipin polymorphisms are risk factors for the development of obesity in adolescents? A case-control study
The Relationship between Serum Zonulin Level and Clinical and Laboratory Parameters of Childhood Obesity
The effect of vitamin D administration on serum leptin and adiponectin levels in end-stage renal disease patients on hemodialysis with vitamin D deficiency: A placebo-controlled double-blind clinical trial
Elective percutaneous coronary intervention leads to significant changes in serum resistin, leptin, and adiponectin levels regardless of periprocedural myocardial injury: an observational study
Altay C, Se?il M, Demir T, Atik T, Ak?nc? G, ?zdemir Kutbay N, Keskin Temelo?lu E, Y?ld?r?m ?im?ir I, ?z???k S, Demir L, Eren E, Tuna EB, Ayta? H, Onay H, Ak?nc? B. Diagn Interv Radiol. 2017 Nov-Dec;23(6):428-434. doi: 10.5152/dir.2017.17019. Dete...
Algul S, Ozdenk C, Ozcelik O. Biol Sport. 2017 Dec;34(4):339-344. doi: 10.5114/biolsport.2017.69821. Epub 2017 Sep 20. Variations in leptin, nesfatin-1 and irisin levels induced by aerobic exercise in young trained and untrained male subjects
He, S., Le, N.A., Frediani, J.K., Winterberg, P.D., Jin, R., Liverman, R.,..., & Vos, M.B. (2017). Cardiometabolic risks vary by weight status in pediatric kidney and liver transplant recipients: A cross-sectional, single-center study in the USA. ...
Acar, S., Paketçi, A., Küme, T., Tuhan, H., Gürsoy Çalan, Ö., Demir, K.,..., & Abacı, A. (2017). Serum galectin-1 levels are positively correlated with body fat and negatively with fasting glucose in obese children. Peptides, 95, 51-56. Advance on...
??atl?? G, An??k A, K??me T, ??alan ??g, D??ndar Bn, B??ber E, Abac?? A. J Endocrinol Invest. 2015 Aug;38(8):909-13. Doi: 10.1007/S40618-015-0277-8. Epub 2015 Apr 2. Serum Nesfatin-1 And Leptin Levels In Non-Obese Girls With Premature Thelarche.
Liu L, Gu H, Zhao Y, An L, Yang J. Exp Ther Med. 2014 Dec;8(6):1813-1818. Epub 2014 Sep 30. Glypican 4 May Be Involved In The Adipose Tissue Redistribution In High-Fat Feeding C57Bl/6J Mice With Peroxisome Proliferators-Activated Receptor ?? Agoni...
Rasekhi H, Karandish M, Jalali Mt, Mohammadshahi M, Zarei M, Saki A, Shahbazian H. J Diabetes Metab Disord. 2015 Jan 14;14(1):1. Doi: 10.1186/S40200-014-0127-9. Ecollection 2015. Phylloquinone Supplementation Improves Glycemic Status Independent O...
Wu Cc, Weng Wl, Lai Wl, Tsai Hp, Liu Wh, Lee Mh, Tsai Yc. Evid Based Complement Alternat Med. 2015;2015:391767. Doi: 10.1155/2015/391767. Epub 2015 Feb 23. Effect Of Lactobacillus Plantarum Strain K21 On High-Fat Diet-Fed Obese Mice.
Karadag As, Ertugrul Dt, Kalkan G, Bilgili Sg, Celik Ht, Takci Z, Balahoroglu R, Calka O. Dermatology. 2013;227(2):103-8. Doi: 10.1159/000351769. Epub 2013 Sep 10. The Effect Of Acitretin Treatment On Insulin Resistance, Retinol-Binding Protein-4,...
Yao Y, Li Xb, Zhao W, Zeng Yy, Shen H, Xiang H, Xiao H. Lipids Health Dis. 2010 May 19;9:49. Doi: 10.1186/1476-511X-9-49. Anti-Obesity Effect Of An Isoflavone Fatty Acid Ester On Obese Mice Induced By High Fat Diet And Its Potential Mechanism.
Xie X, Yang S, Zou Y, Cheng S, Wang Y, Jiang Z, Xiao J, Wang Z, Liu Y. Brain Res. 2013 Jan 23;1491:147-55. Doi: 10.1016/J.Brainres.2012.11.007. Epub 2012 Nov 14. Influence Of The Core Circadian Gene "Clock" On Obesity And Leptin Resistance In Mice.
Hajihashemi P, Azadbakht L, Hashemipor M, Kelishadi R, Esmaillzadeh A. Mol Nutr Food Res. 2014 Jun;58(6):1301-8. Doi: 10.1002/Mnfr.201300582. Epub 2014 Jan 30. Whole-Grain Intake Favorably Affects Markers Of Systemic Inflammation In Obese Children...
Yang Y, Zhang X, Fu Y, Yang H. Toxicol Lett. 2014 Jun 16;227(3):172-8. Doi: 10.1016/J.Toxlet.2014.03.009. Epub 2014 Apr 4. Leptin And Il-8: Two Novel Cytokines Screened Out In Childhood Lead Exposure.
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