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Product Info Summary
| SKU: | EK0437 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
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Product info
Product Name
Human Leptin ELISA Kit PicoKine™
SKU/Catalog Number
EK0437
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human Leptin ELISA Kit PicoKine™ (96 Tests). Quantitate Human LEP in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Leptin ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0437)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: E.coli; Immunogen sequence: V22-C167
Sensitivity
<10 pg/ml
Assay Range
62.5 pg/ml - 4,000 pg/ml
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0437 is reactive to LEP in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK0437 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0437-CAP | Anti-Human LEP Pre-coated 96-well strip microplate | 1 |
| EK0437-ST | Human LEP Standard | 2 vials, 10 ng/tube |
| EK0437-DA | Human LEP Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D. | 0.002 | 0.015 | 0.036 | 0.080 | 0.191 | 0.505 | 0.923 | 1.801 |
Data Example Images
Click image to see more details
Human Leptin PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Leptin/OB, Dilution ratio of 1:10, concentration in serum is 300-16000pg/ml.
Some articles we found to cite concentrations of Leptin/OB in samples: 24900992 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Leptin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 81 | 485 | 1057 | 75 | 444 | 996 |
| Standard deviation | 6.15 | 32.49 | 54.96 | 8 | 35.52 | 69.72 |
| CV (%) | 7.6% | 6.7% | 5.2% | 8.4% | 8% | 7% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Leptin/OB in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 81 | 83 | 84 | 87 | 83 | 2.16 | 2.6% |
| Sample 2 | 485 | 539 | 463 | 476 | 490 | 28.93 | 5.9% |
| Sample 3 | 1057 | 1101 | 1114 | 1116 | 1097 | 23.8 | 2.1% |
Protein Target Info & Infographic
Gene/Protein Information For LEP (Source: Uniprot.Org, NCBI)
Uniprot ID
P41159
Gene ID
3952
Gene Name
LEP
Full Name
Leptin
Weight
18.641kDa
Superfamily
leptin family
Alternative Names
FLJ94114; LEP; leptin (murine obesity homolog); leptin (obesity homolog, mouse); Leptin; OB; Obese protein; obese, mouse, homolog of; Obesity factor; OBOBS LEP LEPD, OB, OBS leptin leptin|leptin (murine obesity homolog)|leptin (obesity homolog, mouse)|obese protein|obese, mouse, homolog of|obesity factor
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on LEP, check out the LEP Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for LEP: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
Specific Publications For Human Leptin ELISA Kit PicoKine™ (EK0437)
EK0437 has been cited in 25 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Turan S,Sarioglu FC,Erbas IM,Cavusoglu B,Karagöz E,Şişman AR,Güney SA,Güleryüz H,Abaci A,Ozturk Y,Akay AP. Altered regional grey matter volume and appetite-related hormone levels in adolescent obesity with or without binge-eating disorder. Eat Weight Diso
Species: Human
EK0437 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA
Leptin partially mediates the association between early-life nutritional supplementation and long-term glycemic status among women in a Guatemalan longitudinal cohort
Adipokines, psoriasis, systemic inflammation, and endothelial dysfunction
Investigation of adropin and leptin levels in pediatric obesity-related nonalcoholic fatty liver disease.
Leptin levels in gingival crevicular fluid during canine retraction: in vivo comparative study
The relation of leptin and soluble leptin receptor levels with metabolic and clinical parameters in obese and healthy children.
Leptin concentration and oxidative stress in diabetic ketoacidosis.
Increased concentrations of serum nesfatin-1 levels in childhood with idiopathic chronic malnutrition
Perilipin polymorphisms are risk factors for the development of obesity in adolescents? A case-control study
The Relationship between Serum Zonulin Level and Clinical and Laboratory Parameters of Childhood Obesity
The effect of vitamin D administration on serum leptin and adiponectin levels in end-stage renal disease patients on hemodialysis with vitamin D deficiency: A placebo-controlled double-blind clinical trial
Elective percutaneous coronary intervention leads to significant changes in serum resistin, leptin, and adiponectin levels regardless of periprocedural myocardial injury: an observational study
Altay C, Se?il M, Demir T, Atik T, Ak?nc? G, ?zdemir Kutbay N, Keskin Temelo?lu E, Y?ld?r?m ?im?ir I, ?z???k S, Demir L, Eren E, Tuna EB, Ayta? H, Onay H, Ak?nc? B. Diagn Interv Radiol. 2017 Nov-Dec;23(6):428-434. doi: 10.5152/dir.2017.17019. Dete...
Algul S, Ozdenk C, Ozcelik O. Biol Sport. 2017 Dec;34(4):339-344. doi: 10.5114/biolsport.2017.69821. Epub 2017 Sep 20. Variations in leptin, nesfatin-1 and irisin levels induced by aerobic exercise in young trained and untrained male subjects
He, S., Le, N.A., Frediani, J.K., Winterberg, P.D., Jin, R., Liverman, R.,..., & Vos, M.B. (2017). Cardiometabolic risks vary by weight status in pediatric kidney and liver transplant recipients: A cross-sectional, single-center study in the USA. ...
Acar, S., Paketçi, A., Küme, T., Tuhan, H., Gürsoy Çalan, Ö., Demir, K.,..., & Abacı, A. (2017). Serum galectin-1 levels are positively correlated with body fat and negatively with fasting glucose in obese children. Peptides, 95, 51-56. Advance on...
??atl?? G, An??k A, K??me T, ??alan ??g, D??ndar Bn, B??ber E, Abac?? A. J Endocrinol Invest. 2015 Aug;38(8):909-13. Doi: 10.1007/S40618-015-0277-8. Epub 2015 Apr 2. Serum Nesfatin-1 And Leptin Levels In Non-Obese Girls With Premature Thelarche.
Liu L, Gu H, Zhao Y, An L, Yang J. Exp Ther Med. 2014 Dec;8(6):1813-1818. Epub 2014 Sep 30. Glypican 4 May Be Involved In The Adipose Tissue Redistribution In High-Fat Feeding C57Bl/6J Mice With Peroxisome Proliferators-Activated Receptor ?? Agoni...
Rasekhi H, Karandish M, Jalali Mt, Mohammadshahi M, Zarei M, Saki A, Shahbazian H. J Diabetes Metab Disord. 2015 Jan 14;14(1):1. Doi: 10.1186/S40200-014-0127-9. Ecollection 2015. Phylloquinone Supplementation Improves Glycemic Status Independent O...
Wu Cc, Weng Wl, Lai Wl, Tsai Hp, Liu Wh, Lee Mh, Tsai Yc. Evid Based Complement Alternat Med. 2015;2015:391767. Doi: 10.1155/2015/391767. Epub 2015 Feb 23. Effect Of Lactobacillus Plantarum Strain K21 On High-Fat Diet-Fed Obese Mice.
Karadag As, Ertugrul Dt, Kalkan G, Bilgili Sg, Celik Ht, Takci Z, Balahoroglu R, Calka O. Dermatology. 2013;227(2):103-8. Doi: 10.1159/000351769. Epub 2013 Sep 10. The Effect Of Acitretin Treatment On Insulin Resistance, Retinol-Binding Protein-4,...
Yao Y, Li Xb, Zhao W, Zeng Yy, Shen H, Xiang H, Xiao H. Lipids Health Dis. 2010 May 19;9:49. Doi: 10.1186/1476-511X-9-49. Anti-Obesity Effect Of An Isoflavone Fatty Acid Ester On Obese Mice Induced By High Fat Diet And Its Potential Mechanism.
Xie X, Yang S, Zou Y, Cheng S, Wang Y, Jiang Z, Xiao J, Wang Z, Liu Y. Brain Res. 2013 Jan 23;1491:147-55. Doi: 10.1016/J.Brainres.2012.11.007. Epub 2012 Nov 14. Influence Of The Core Circadian Gene "Clock" On Obesity And Leptin Resistance In Mice.
Hajihashemi P, Azadbakht L, Hashemipor M, Kelishadi R, Esmaillzadeh A. Mol Nutr Food Res. 2014 Jun;58(6):1301-8. Doi: 10.1002/Mnfr.201300582. Epub 2014 Jan 30. Whole-Grain Intake Favorably Affects Markers Of Systemic Inflammation In Obese Children...
Yang Y, Zhang X, Fu Y, Yang H. Toxicol Lett. 2014 Jun 16;227(3):172-8. Doi: 10.1016/J.Toxlet.2014.03.009. Epub 2014 Apr 4. Leptin And Il-8: Two Novel Cytokines Screened Out In Childhood Lead Exposure.
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Customer Reviews
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Customer Q&As
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17 Customer Q&As for Human Leptin ELISA Kit PicoKine™
Question
Do you have any information available documenting the effects of aprotinin on the ELISA results using EK0437?
Verified customer
Asked: 2020-12-01
Answer
Our lab hasn't validated effects of aprotinin on the ELISA results using the Human Leptin ELISA Kit PicoKine™ (EK0437). Please run a pilot test to see if it has any effect.
Boster Scientific Support
Answered: 2020-12-01
Question
We have acquired the products leptin (EK0437), adiponectin (EK0595) and endothelin (EK0945) from BOSTER and our sample type is humans' plasma (EDTA). We have read the respective datasheets. Even so, we need helping, since we have not conditions to test sample dilution proportion. What dilution ratio do you suggest for using with these kits? 1:10, 1:100, 1:1000 or another one? Please, give us numbers.
Verified Customer
Verified customer
Asked: 2020-02-28
Answer
Please see the following recommended dilution ratios for plasma (EDTA) from normal healthy human. leptin (EK0437), 1:20 adiponectin (EK0595) around 1:2000 endothelin (EK0945) 1:1
Boster Scientific Support
Answered: 2020-02-28
Question
Dear Technical Support: I am interested with Human Leptin ELISA Kit PicoKine for human population. My query is; how does these Human Leptin ELISA Kit PicoKine work well for urine sample in human adolescent? Is it required to concentrate the sample in order to measure the hormone?
Verified Customer
Verified customer
Asked: 2020-01-27
Answer
The product Human Leptin ELISA Kit PicoKine has not been tested particularly in human adolescents, however the typical human urine does not need to be concentrated and can be diluted using these Human Leptin ELISA Kit PicoKine if necessary.
Boster Scientific Support
Answered: 2020-01-27
Question
Q: if the enzyme conjugated Leptin antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Leptin antigen?
R. Allen
Verified customer
Asked: 2019-12-05
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2019-12-05
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2019-10-11
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2019-10-11
Question
What is the suggested dilution ratio for cell culture supernates using the Human Leptin ELISA Kit PicoKine™ (EK0437)?
Verified customer
Asked: 2019-09-19
Answer
For cell culture supernates, concentrations of the samples can vary greatly. We suggest to run pilot tests for different group samples to figure out the optimal dilution ratio. Our lab tested unstimulated human peripheral blood mononuclear cells with a dilution of 1:1 and didn't detect any measurable value.
Boster Scientific Support
Answered: 2019-09-20
Question
What is the suggested dilution ratio for cell lysates using the Human Leptin ELISA Kit PicoKine™ (EK0437)?
Verified customer
Asked: 2019-09-19
Answer
For cell lysates, concentrations of the samples can vary greatly. We suggest to run pilot tests for different group samples to figure out the optimal dilution ratio. Our lab tested unstimulated human peripheral blood mononuclear cells with a dilution of 1:1 and didn't detect any measurable value.
Boster Scientific Support
Answered: 2019-09-20
Question
What is the suggested dilution ratio for serum and plasma (heparin, EDTA) using the Human Leptin ELISA Kit PicoKine™ (EK0437)?
Verified customer
Asked: 2019-09-19
Answer
For serum and plasma (heparin, EDTA), the suggested dilution ratio from normal healthy human is 1:20.
Boster Scientific Support
Answered: 2019-09-20
Question
I want to purchase your Human Leptin ELISA Kit PicoKine, but I have some questions; does this kit would be useful in rabbit serum?
Verified Customer
Verified customer
Asked: 2019-08-15
Answer
The product Human Leptin ELISA Kit PicoKine have not been tested for any cross reactivity with rabbit samples, thus we do not know if the kit will work with rabbit serum samples. In this kit both capture and detection antibody are monoclonal so unless the homology between human and rabbit is very high, there would be a possibility that it will cross react, but we cannot guarantee.
Boster Scientific Support
Answered: 2019-08-15
Question
Q: What is the optimal O.D. value for Leptin ELISA kit? I used your Leptin ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain Leptin even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2019-06-20
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Leptin ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of Leptin that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2019-06-20
Question
Please help with this. On page 6 of the attached insert...it states the dilution of sample must be made with the sample diluent. But it does not state the target dilutions to be prepared by the user. I looked through this on the attached insert but also cannot understand. Please clarify for me asap Sample Dilution The target protein concentration should be estimated and appropriate sample dilutions should be selected such that the final protein concentration lies near the middle of the linear dynamic range of the assay. It is recommended to prepare 150 ?l of sample for each replicate to be assayed. The samples should be diluted with sample diluent and mixed gently.
Verified Customer
Verified customer
Asked: 2019-06-07
Answer
Please see the following recommended dilution ratios for Human Leptin PicoKine
Boster Scientific Support
Answered: 2019-06-07
Question
Q: Can Leptin ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
S. Garcia
Verified customer
Asked: 2019-05-29
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is a must. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2019-05-29
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2019-01-14
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can change immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2019-01-14
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis Leptin using the EK0437 Human Leptin Picokine® ELISA Kit?
D. Varadkar
Verified customer
Asked: 2018-09-19
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples to prevent protein degradation.
Boster Scientific Support
Answered: 2018-09-19
Question
Q: can I use citrate plasma as samples in Human Leptin Picokine® ELISA Kit (Catalog # EK0437)?
Verified Customer
Verified customer
Asked: 2018-04-16
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of Leptin causing degradation of its protein structure. Leptin may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the Leptin ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2018-04-16
Question
Q: which procedure should I follow in order to thaw whole blood sample for Leptin ELISA after freezing?
Verified Customer
Verified customer
Asked: 2016-06-17
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Leptin for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2016-06-17
Question
EK0437 Human Leptin PicoKine ELISA Kit I have a customer who is interested in the kit. They want to test it with human serum samples. Please provide your QC data for the current lot of kit along with reference values for serum samples.
Verified Customer
Verified customer
Asked: 2015-05-19
Answer
COA
Boster Scientific Support
Answered: 2015-05-19
