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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum and plasma (heparin, EDTA).|
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Human LY9 ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human LY9 ELISA Kit PicoKine™ (96 Tests). Quantitate Human LY9 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 15pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human LY9 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1932)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: K48-K454
78 pg/ml - 5,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK1932 is reactive to LY9 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
EK1932 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of CD229/SLAMF3/Lymphocyte Antigen 9
T-lymphocyte surface antigen Ly-9 is a protein that in humans is encoded by the LY9 gene. This gene is mapped to 17q21.31. LY9 has also recently been designated CD229. LY9 belongs to the SLAM family of immunomodulatory receptors and interacts with the adaptor molecule SAP.
|EK1932-CAP||Anti-Human LY9 Pre-coated 96-well strip microplate||1|
|EK1932-ST||Human LY9 Standard||2 vials, 10 ng/tube|
|EK1932-DA||Human LY9 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human LY9 PicoKine ELISA Kitstandard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect CD229/SLAMF3/Lymphocyte Antigen 9, Dilution ratio of 1:1, concentration in serum is around 1000pg/ml.
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human LY9 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of CD229/SLAMF3/Lymphocyte Antigen 9 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For LY9 (Source: Uniprot.Org, NCBI)
T-lymphocyte surface antigen Ly-9
CD229 antigen; CD229; cell-surface molecule Ly-9; hly9; Ly9; lymphocyte antigen 9Cell surface molecule Ly-9; mLY9; SLAMF3; T-lymphocyte surface antigen Ly-9 LY9 CD229, SLAMF3, hly9, mLY9 lymphocyte antigen 9 T-lymphocyte surface antigen Ly-9|SLAM family member 3|cell surface molecule Ly-9|signaling lymphocytic activation molecule 3*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on LY9, check out the LY9 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for LY9: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
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