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Product Info Summary
| SKU: | EK2061 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA, citrate). |
Product info
Product Name
Human MAG ELISA Kit PicoKine®
View all MAG/Siglec-4a ELISA kits
SKU/Catalog Number
EK2061
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human MAG ELISA Kit PicoKine™ (96 Tests). Quantitate Human MAG in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 50pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human MAG ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2061)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: G20-P516
Sensitivity
<50 pg/ml
Assay Range
312 pg/ml - 20,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK2061 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2061 is reactive to MAG in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
Application Guarantee
EK2061 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of MAG/Siglec-4a
Myelin-associated glycoprotein (MAG, Siglec-4) is a cell membrane glycoprotein that is a member of the SIGLEC family of proteins and is a functional ligand of the NoGo-66 receptor (NgR). This gene is mapped to 19q13.12. The protein encoded by this gene is a type I membrane protein and member of the immunoglobulin superfamily. It is thought to be involved in the process of myelination. It is a lectin that binds to sialylated glycoconjugates and mediates certain myelin-neuron cell-cell interactions. Three alternatively spliced transcripts encoding different isoforms have been described for this gene.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2061-CAP | Anti-Human MAG Pre-coated 96-well strip microplate | 1 |
| EK2061-ST | Human MAG Standard | 2 vials, 20 ng/tube |
| EK2061-DA | Human MAG Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 312 | 625 | 1250 | 2500 | 5000 | 10000 | 20000 |
| O.D. | 0.055 | 0.125 | 0.169 | 0.299 | 0.545 | 0.956 | 1.437 | 2.064 |
Data Example Images
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Human MAG PicoKine ELISA Kit Standard Curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human MAG ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 384 | 2313 | 8445 | 492 | 2436 | 9458 |
| Standard deviation | 17.28 | 131.84 | 388.47 | 25.09 | 143.72 | 699.89 |
| CV (%) | 4.5% | 5.7% | 4.6% | 5.1% | 5.9% | 7.4% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of MAG/Siglec-4a in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 384 | 401 | 375 | 392 | 388 | 9.61 | 2.4% |
| Sample 2 | 2313 | 1997 | 2156 | 1982 | 2112 | 134.59 | 6.3% |
| Sample 3 | 8445 | 7435 | 7826 | 7234 | 7735 | 461.89 | 5.9% |
Protein Target Info & Infographic
Gene/Protein Information For MAG (Source: Uniprot.Org, NCBI)
Gene Name
MAG
Full Name
Myelin-associated glycoprotein
Weight
69.069kDa
Superfamily
immunoglobulin superfamily
Alternative Names
GMAsialic acid binding Ig-like lectin 4A; MAG; myelin associated glycoprotein; myelin-associated glycoprotein; sialic acid-binding immunoglobulin-like lectin 4A; Siglec4a; Siglec-4a; S-MAG MAG GMA, S-MAG, SIGLEC-4A, SIGLEC4A, SPG75 myelin associated glycoprotein myelin-associated glycoprotein|sialic acid binding Ig-like lectin 4A|sialic acid-binding immunoglobulin-like lectin 4A
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on MAG, check out the MAG Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for MAG: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human MAG ELISA Kit PicoKine® (EK2061)
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No publications found for EK2061
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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8 Customer Q&As for Human MAG ELISA Kit PicoKine®
Question
Q: if the enzyme conjugated MAG antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the MAG antigen?
Verified Customer
Verified customer
Asked: 2020-05-20
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2020-05-20
Question
Q: how are cell lysates prepared for use in Picokine® ELISA kits?
K. Rodriguez
Verified customer
Asked: 2020-04-29
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2020-04-29
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2019-02-24
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2019-02-24
Question
Q: What is the optimal O.D. value for MAG ELISA kit? I used your MAG ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain MAG even though the O.D. values are not very high?
B. Taylor
Verified customer
Asked: 2018-10-27
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the example mentioned, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this MAG ELISA kit to have sensitivity of 50pg/ml, that means the minimum amount of MAG that can be declared/interpreted as positive by the above standard is 50pg/ml.
Boster Scientific Support
Answered: 2018-10-27
Question
Q: Can MAG ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
L. Chen
Verified customer
Asked: 2018-03-27
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is needed. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2018-03-27
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2017-05-18
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2017-05-18
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
R. Lopez
Verified customer
Asked: 2017-04-05
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is a must for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2017-04-05
Question
Q: is it okay to use heparin plasma as samples in Human MAG Picokine® ELISA Kit (Catalog # EK2061)?
A. Young
Verified customer
Asked: 2014-12-14
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of MAG causing disruption of its protein structure. MAG may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the MAG ELISA, treating samples with different anticoagulants and decided that heparin, EDTA or citrate can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2014-12-14
