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Product Info Summary
| SKU: | EK0941 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum, plasma (heparin, EDTA) and saliva. |
Product info
Product Name
Human MIA ELISA Kit PicoKine®
SKU/Catalog Number
EK0941
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human MIA ELISA Kit PicoKine™ (96 Tests). Quantitate Human MIA in cell culture supernatants, serum, plasma (heparin, EDTA) and saliva. Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human MIA ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0941)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: E.coli; Immunogen sequence: G25-131Q
Sensitivity
<10 pg/ml
Assay Range
15.6 pg/ml - 1,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK0941 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0941 is reactive to MIA in Human samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin, EDTA) and saliva.
Application Guarantee
EK0941 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of MIA
Melanoma Inhibiting Activity (MIA), also known as cartilage-derived retinoic acid-sensitive protein (CD-RAP), is an approximately 11-15 kDa protein that is expressed as a noncovalent homodimer. MIA is structurally related to OTOR/Otoraplin and MIA-2 in a small family of secreted proteins with one SH3 domain (1-3). And the MIA gene was mapped to 19q13.32-q13.33 by fluorescence in situ hybridization. Beside, It is a marker for malignant melanoma. MIA functions as a chemoattractant for mesenchymal stem cells and enhances their BMP-2 and TGF-beta3 induced differentiation into chondrocytes . MIA-deficient mice exhibit delayed chondrocyte differentiation but enhanced chondrocyte proliferation and cartilage repair .
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0941-CAP | Anti-Human MIA Pre-coated 96-well strip microplate | 1 |
| EK0941-ST | Human MIA Standard | 2 vials, 1 ng/tube |
| EK0941-DA | Human MIA Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.021 | 0.054 | 0.089 | 0.156 | 0.352 | 0.892 | 1.396 | 2.046 |
Data Example Images
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Human MIA PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect MIA, Dilution ratio of 1:10, concentration in serum and plasma is around 2 ng/ml..
Some articles we found to cite concentrations of MIA in samples: 15906071 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human MIA ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 29 | 172 | 485 | 26 | 165 | 509 |
| Standard deviation | 1.91 | 13.41 | 32.98 | 1.97 | 15.84 | 36.92 |
| CV (%) | 6.6% | 7.8% | 6.8% | 7.6% | 9.6% | 6.9% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of MIA in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 29 | 28 | 27 | 29 | 28 | 0.82 | 2.9% |
| Sample 2 | 172 | 166 | 171 | 170 | 169 | 2.27 | 1.3% |
| Sample 3 | 485 | 453 | 511 | 458 | 476 | 23.22 | 4.8% |
Protein Target Info & Infographic
Gene/Protein Information For MIA (Source: Uniprot.Org, NCBI)
Gene Name
MIA
Full Name
Melanoma-derived growth regulatory protein
Weight
14.509kDa
Superfamily
MIA/OTOR family
Alternative Names
CD-RAP; Melanoma inhibitory activity protein; melanoma inhibitory activity; melanoma-derived growth regulatory protein; MIA MIA CD-RAP MIA SH3 domain containing melanoma-derived growth regulatory protein|melanoma inhibitory activity
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on MIA, check out the MIA Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for MIA: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human MIA ELISA Kit PicoKine® (EK0941)
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Customer Q&As
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8 Customer Q&As for Human MIA ELISA Kit PicoKine®
Question
Can standard Dulbecco's Phosphate Buffered Saline(contains KCl and MgCl) be used instead of the 0.01 M PBS in EK0941?
Verified customer
Asked: 2020-12-01
Answer
The standard Dulbecco's Phosphate Buffered Saline(contains KCl and MgCl) can be used as wash buffer for the Human MIA ELISA Kit PicoKine™ (EK0941).
Boster Scientific Support
Answered: 2020-12-02
Question
Q: What is the optimal O.D. value for MIA ELISA kit? I performed your MIA ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain MIA even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2019-12-31
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the above example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this MIA ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of MIA that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2019-12-31
Question
Q: is it okay to use citrate plasma as samples in Human MIA Picokine® ELISA Kit (Catalog # EK0941)?
Verified Customer
Verified customer
Asked: 2019-08-21
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of MIA causing disruption of its protein structure. MIA may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the MIA ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2019-08-21
Question
Q: how can I thaw whole blood sample for MIA ELISA after freezing?
Verified Customer
Verified customer
Asked: 2019-05-16
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test MIA for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2019-05-16
Question
Q: how do I analyze ELISA data? I have obtained MIA level in serum.
Verified Customer
Verified customer
Asked: 2018-06-07
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2018-06-07
Question
Q: if the enzyme conjugated MIA antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the MIA antigen?
J. Collins
Verified customer
Asked: 2018-02-19
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2018-02-19
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
K. Nelson
Verified customer
Asked: 2017-12-18
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2017-12-18
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
V. Krishna
Verified customer
Asked: 2017-06-27
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2017-06-27
