Human MIA ELISA Kit PicoKine™
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human MIA. 96wells/kit, with removable strips.
Human MIA ELISA Kit PicoKine™ Info At A Glance
|Size:||96wells/kit, with removable strips.|
|Sample Type:||cell culture supernates, serum, plasma(heparin, EDTA) and saliva.|
|Sample Volume:||100μl per well|
|Product Name||Human MIA ELISA Kit PicoKine™
See all MIA primary antibodies, ELISA kits and proteins
|Storage & Handling||Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)|
|Description||Detect Human MIA with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, serum, plasma(heparin, EDTA) and saliva. This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. MIA tissue specificity: All malignant melanoma cell lines tested and infrequently in glioma cell lines.|
|Cite This Product||Human MIA ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0941)|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab.
|Sample Type||cell culture supernates, serum, plasma(heparin, EDTA) and saliva.|
|Immunogen/Standard||Expression system for standard: E.coli; Immunogen sequence: G25-131Q|
|Antibody Clonalities||Capture antibody|Detection antibody
monoclonal antibody from mouse|polyclonal antibody from goat
|Cross Reactivity||There is no detectable cross-reactivity with other relevant proteins.|
|Pack Size||96wells/kit, with removable strips.|
Gene/Protein Basic Information For MIA (Source: Uniprot.org, NCBI)
|NCBI Gene Id||8190|
|Species Of This Entry||Human|
|Protein Name||Melanoma-derived growth regulatory protein|
|Alternative Names||MIA|CD-RAP; Melanoma inhibitory activity protein; melanoma inhibitory activity; melanoma-derived growth regulatory protein; MIA|
|Post Tranlational Modifications||Phosphorylation; Cleavage; Methylation; Dephosphorylation; Oxidation; Reduction; Farnesylation; Acetylation; Demethylation; Prenylation; Glycosylation; Carboxylation; Phosphorothioation; Glutathionylation; Deamination; Acylation; Ribosylation; Deamidation; Ubiquitination; Iodination|
|Gene Location||19q13.2, on Chromasome 19, gene sequence: NC_000019.10 (40775160..40777490)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For MIA (Source: Uniprot.org, NCBI)
|Protein Function||Elicits growth inhibition on melanoma cells in vitro as well as some other neuroectodermal tumors, including gliomas.|
|Tissue Specificity||All malignant melanoma cell lines tested and infrequently in glioma cell lines.|
|Related Diseases||Malignant Neoplasms; Malignant Neoplasm Of Pancreas; Pancreatic Neoplasm; Neoplasms; Carcinoma; Melanoma; Adenocarcinoma; Neoplasm Metastasis; Malignant Paraganglionic Neoplasm; Degenerative Polyarthritis; Cell Invasion; Pain; Pancreatic Adenocarcinoma; Skin Neoplasms; Neoplasm Invasiveness; Inflammation; Tissue Adhesions; Malignant Neoplasm Of Breast; Liver Neoplasms|
|Related Pathways||Cell Growth; Cell Proliferation; Cell Cycle; Cell Death; Secretion; Transport; Localization; Angiogenesis; Cell Cycle Arrest; Induction Of Apoptosis; Pathogenesis; Sensitization; Cell Migration; Cell Adhesion; Translation; Glycolysis; Hypersensitivity; Regeneration; S Phase; Rna Interference|
|Background||Melanoma Inhibiting Activity(MIA), also known as cartilage-derived retinoic acid-sensitive protein(CD-RAP), is an approximately 11-15 kDa protein that is expressed as a noncovalent homodimer. MIA is structurally related to OTOR/Otoraplin and MIA-2 in a small family of secreted proteins with one SH3 domain(1-3). And the MIA gene was mapped to 19q13.32-q13.33 by fluorescence in situ hybridization. Beside, It is a marker for malignant melanoma. MIA functions as a chemoattractant for mesenchymal stem cells and enhances their BMP-2 and TGF-beta3 induced differentiation into chondrocytes . MIA-deficient mice exhibit delayed chondrocyte differentiation but enhanced chondrocyte proliferation and cartilage repair .|
|Scientific References||PMID: 7923218 by Blesch A., et al. Cloning of a novel malignant melanoma-derived growth-regulatory protein, MIA.|
PMID: 8550608 by Bosserhoff A.-K., et al. Structure and promoter analysis of the gene encoding the human melanoma-inhibiting protein MIA.
Kit Components And Assay QC Data Details Of Human MIA ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
|EK0941-CAP||96-well plate precoated with anti-Human MIA antibody||1|
|EK0941-ST||lyophilized recombinant Human MIA standard||1ng/tube|
|EK0941-DA||biotinylated anti-Human MIA antibody||130ul|
|AR1106-1||sample diluent buffer||30ml|
|AR1106-2||antibody diluent buffer||12ml|
|AR1106-3||ABC diluent buffer||12ml|
|AR1104||TMB color developing agent||10ml|
|AR1105||TMB stop solution||10ml|
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EK0941 Human MIA ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
We measured random samples of Human MIA ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We measured the performance consistency of Human MIA ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
|Lots||Lot1 (pg/ml)||Lot2 (pg/ml)||Lot3 (pg/ml)||Lot4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
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