Human MIF ELISA Kit PicoKine™

Human MIF ELISA Kit PicoKine™ (96 Tests). Quantitate Human MIF in cell culture supernatants, serum, plasma (heparin, EDTA) and human milk. Sensitivity: 20pg/ml. Cited in 1 publication(s).

Product Info Summary

SKU: EK0813
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum, plasma (heparin, EDTA) and human milk.

Product Name

Human MIF ELISA Kit PicoKine™

See all MIF products

SKU/Catalog Number

EK0813

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human MIF ELISA Kit PicoKine™ (96 Tests). Quantitate Human MIF in cell culture supernatants, serum, plasma (heparin, EDTA) and human milk. Sensitivity: 20pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human MIF ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0813)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: P2-A115

Sensitivity

<20 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0813 is reactive to MIF in Human samples

Validated Sample Types

cell culture supernatants, serum, plasma (heparin, EDTA) and human milk.

Application Guarantee

EK0813 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0813-CAP Anti-Human MIF Pre-coated 96-well strip microplate 1
EK0813-ST Human MIF Standard 2 vials, 10 ng/tube
EK0813-DA Human MIF Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631262512502500500010000
O.D.0.0230.1120.1980.3180.6111.1001.6962.117

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect MIF, Dilution ratio of 1:5, concentration in serum is around 5ng/ml.

Some articles we found to cite concentrations of MIF in samples: 22846129, 25328446 (Pubmed IDs).

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human MIF ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)2801655455930516944885
Standard deviation12.0466.2206.3516.4777.92307.75
CV (%)4.3%4%4.5%5.4%4.6%6.3%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of MIF in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 12802662562732688.873.3%
Sample 21655156217831751168786.545.1%
Sample 345594531389946124400290.856.6%
*number of samples for each test n=16.

Gene/Protein Information For MIF (Source: Uniprot.Org, NCBI)

Uniprot ID

P14174

Gene ID

4282

Gene Name

MIF

Full Name

Macrophage migration inhibitory factor

Weight

12.476kDa

Superfamily

MIF family

Alternative Names

EC 5.3.2.1; EC 5.3.3.12; GIFmacrophage migration inhibitory factor; GLIF; Glycosylation-inhibiting factor; L-dopachrome isomerase; L-dopachrome tautomerase; macrophage migration inhibitory factor (glycosylation-inhibiting factor); MIF; MMIF; Phenylpyruvate tautomerase MIF GIF, GLIF, MMIF macrophage migration inhibitory factor macrophage migration inhibitory factor|L-dopachrome isomerase|L-dopachrome tautomerase|epididymis secretory sperm binding protein|macrophage migration inhibitory factor (glycosylation-inhibiting factor)|phenylpyruvate tautomerase

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on MIF, check out the MIF Infographic

MIF infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for MIF: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0813 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Farag AGA, Hammam MA, Habib MS, Elnaidany NF, Kamh ME. An Bras Dermatol. 2018 Mar;93(2):191-196. doi: 10.1590/abd1806-4841.20186068. Macrophage migration inhibitory factor as an incriminating agent in vitiligo*

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7 Customer Q&As for Human MIF ELISA Kit PicoKine™

Question

Can this kit be used on plasma collected with citrate instead of heparin or EDTA?

Verified Customer

Verified customer

Asked: 2020-04-28

Answer

We have not validated this kit with plasma collected with citrate. However, you can try to determine if the kit would work for your sample.

Boster Scientific Support

Answered: 2020-04-28

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

K. Roberts

Verified customer

Asked: 2019-05-25

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2019-05-25

Question

Q: Can MIF ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

Verified Customer

Verified customer

Asked: 2019-04-03

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2019-04-03

Question

Q: if the enzyme conjugated MIF antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the MIF antigen?

Verified Customer

Verified customer

Asked: 2019-02-25

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2019-02-25

Question

Q: What is the optimal O.D. value for MIF ELISA kit? I used your MIF ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain MIF even though the O.D. values are not very high?

B. Sunder

Verified customer

Asked: 2017-12-31

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the above example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this MIF ELISA kit to have sensitivity of 20pg/ml, that means the minimum amount of MIF that can be declared/interpreted as positive by the above standard is 20pg/ml.

Boster Scientific Support

Answered: 2017-12-31

Question

Q: how are cell lysates prepared for use in Picokine® ELISA kits?

S. Parker

Verified customer

Asked: 2016-10-08

Answer

A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2016-10-08

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

H. Davis

Verified customer

Asked: 2016-05-08

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2016-05-08

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