Human sE-Selectin / CD62E ELISA Kit PicoKine®

E-Selectin/CD62E ELISA kit for Human

Human sE-Selectin / CD62E ELISA Kit PicoKine™ (96 Tests). Quantitate Human SELE in cell culture supernatants, serum and plasma (heparin, citrate). Sensitivity: 4pg/ml. Cited in 10 publication(s).

Product Info Summary

SKU: EK0501
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, citrate).

Product Name

Human sE-Selectin / CD62E ELISA Kit PicoKine®

View all E-Selectin/CD62E ELISA kits

SKU/Catalog Number

EK0501

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human sE-Selectin / CD62E ELISA Kit PicoKine™ (96 Tests). Quantitate Human SELE in cell culture supernatants, serum and plasma (heparin, citrate). Sensitivity: 4pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human sE-Selectin / CD62E ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0501)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: W22-P556

Sensitivity

<4 pg/ml

Assay Range

125 pg/ml - 8,000 pg/ml

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0501 is reactive to SELE in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, citrate).

Application Guarantee

EK0501 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of E-Selectin/CD62E

E-selectin, also called endothelial-leukocyte adhesion molecule-1 (ELAM-1), is a cell surface glycoprotein expressed by cytokine-activated endothelium that mediates the adhesion of blood neutrophils. An increased expression of E-selectin has been observed in the arterial endothelium interacting with lymphocytes and macrophages in human atherosclerotic lesions. E-selectin plays a critical role in mediating tissue-specific homing of T cells into skin, and of primitive hematopoietic progenitor cells (HPCs) into bone marrow (BM). A structurally and functionally related group of genes, lymph node homing receptor (LHR), granule membrane protein 140 (GMP-140), and endothelial leukocyte adhesion molecule 1 (ELAM-1) are shown to constitute a gene cluster on mouse and human chromosome 1. The standard product used in this kit is recombinant human E-Selectin, excluding intercellular E-Selectin and transmembrane domain. It consists of totally 535 amino acids with the molecular mass of 58.6KDa.

Kit Components

Catalog Number Description Quantity
EK0501-CAP Anti-Human SELE Pre-coated 96-well strip microplate 1
EK0501-ST Human SELE Standard 2 vials, 10 ng/tube
EK0501-DA Human SELE Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)01252505001000200040008000
O.D.0.0110.0860.1770.4140.6831.1971.6612.024

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human sE-Selectin / CD62E ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)1521179514713810815271
Standard deviation1270.74303.6711.0476.75390.05
CV (%)7.9%6%5.9%8%7.1%7.4%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of E-Selectin/CD62E in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 11521501491471491.81.2%
Sample 21179123312211130119040.43.3%
Sample 351475002582459045469399.067.2%
*number of samples for each test n=16.

Gene/Protein Information For SELE (Source: Uniprot.Org, NCBI)

Uniprot ID

P16581

Gene ID

6401

Gene Name

SELE

Full Name

E-selectin

Weight

66.655kDa

Superfamily

selectin/LECAM family

Alternative Names

CD62 antigen-like family member E; CD62E antigen; CD62E; ELAM; ELAM1; ELAM-1; ELAM1E-selectin; endothelial adhesion molecule 1; Endothelial leukocyte adhesion molecule 1; ESEL; E-Selectin; LECAM2; leukocyte endothelial cell adhesion molecule 2; Leukocyte-endothelial cell adhesion molecule 2; SELE; selectin E SELE CD62E, ELAM, ELAM1, ESEL, LECAM2 selectin E E-selectin|CD62 antigen-like family member E|ELAM-1|endothelial adhesion molecule 1|endothelial leukocyte adhesion molecule 1|leukocyte endothelial cell adhesion molecule 2

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on SELE, check out the SELE Infographic

SELE infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for SELE: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0501 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

CFTR ameliorates high glucose-induced oxidative stress and inflammation by mediating the NF-κB and MAPK signaling pathways in endothelial cells

FGF-23/Vitamin D Axis in Type 1 Diabetes: The Potential Role of Mineral Metabolism in Arterial Stiffness

Globular adiponectin counteracts VCAM-1-mediated monocyte adhesion via AdipoR1/NF-κB/COX-2 signaling in human aortic endothelial cells

Haptoglobin genotype is associated with increased endothelial dysfunction serum markers in type 1 diabetes

MicroRNA-181a-5p and microRNA-181a-3p cooperatively restrict vascular inflammation and atherosclerosis

Improvement of hypertension, endothelial function and systemic inflammation following short-term supplementation with red beet ( Beta vulgaris L.) juice: a randomized crossover pilot study

A novel anti-inflammatory mechanism of high density lipoprotein through up-regulating annexin A1 in vascular endothelial cells

Haptoglobin genotype is associated with increased endothelial dysfunction serum markers in type 1 diabetes.

Neurally mediated syncope: Is it really an endothelial dysfunction?

Metoprolol Improves Endothelial Function in Patients with Cardiac Syndrome X

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8 Customer Q&As for Human sE-Selectin / CD62E ELISA Kit PicoKine®

Question

Q: how to proceed with the analysis of ELISA data? I have performed CD62E level in plasma.

Verified Customer

Verified customer

Asked: 2020-10-08

Answer

A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2020-10-08

Question

Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification CD62E using the EK0501 Human CD62E Picokine® ELISA Kit?

D. Varadkar

Verified customer

Asked: 2020-03-04

Answer

A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to avoid protein degradation.

Boster Scientific Support

Answered: 2020-03-04

Question

Q: if the enzyme conjugated CD62E antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the CD62E antigen?

G. Babu

Verified customer

Asked: 2020-01-12

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2020-01-12

Question

Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2018-05-15

Answer

A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer may affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2018-05-15

Question

Q: What is the optimal O.D. value for CD62E ELISA kit? I used your CD62E ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain CD62E even though the O.D. values are not very high?

M. Rodriguez

Verified customer

Asked: 2017-11-16

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this CD62E ELISA kit to have sensitivity of 4pg/ml, that means the minimum amount of CD62E that can be declared/interpreted as positive by the above standard is 4pg/ml.

Boster Scientific Support

Answered: 2017-11-16

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2017-03-21

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2017-03-21

Question

Q: Can CD62E ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

Verified Customer

Verified customer

Asked: 2016-03-18

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is a must. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2016-03-18

Question

Is this kit specifically validated (only detect E-selectin no other selectin family members such as L or P selection).?

Verified Customer

Verified customer

Asked: 2013-01-01

Answer

EK0501 is specific to E-Selectin. No significant cross reactivity was observed with recombinant P­-Selectin, L­-Selectin, ICAM­1 and VCAM­1.

Boster Scientific Support

Answered: 2013-01-01

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