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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum and plasma (heparin).|
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Human SPINK1/TATI ELISA Kit PicoKine™
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human SPINK1/TATI ELISA Kit PicoKine™ (96 Tests). Quantitate Human SPINK1 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Cite This Product
Human SPINK1/TATI ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1241)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: E.coli; Immunogen sequence: D24-C79
156 pg/ml - 10,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK1241 is reactive to SPINK1 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin).
EK1241 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
|EK1241-CAP||Anti-Human SPINK1 Pre-coated 96-well strip microplate||1|
|EK1241-ST||Human SPINK1 Standard||2 vials, 10 ng/tube|
|EK1241-DA||Human SPINK1 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human SPINK1/TATI PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect SPINK1, Dilution ratio of 1:1, concentration in serum is around 2ng/ml.
Some articles we found to cite concentrations of SPINK1 in samples: 24254562 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human SPINK1/TATI ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of SPINK1 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For SPINK1 (Source: Uniprot.Org, NCBI)
Serine protease inhibitor Kazal-type 1
pancreatic secretory trypsin inhibitor; PCTT; PCTTSpink3; PSTI; PSTISerine protease inhibitor Kazal-type 1; serine peptidase inhibitor, Kazal type 1; serine protease inhibitor, Kazal type 1; SPINK1; Spink3; TATI; TATITumor-associated trypsin inhibitor SPINK1 PCTT, PSTI, Spink3, TATI, TCP serine peptidase inhibitor Kazal type 1 serine protease inhibitor Kazal-type 1|pancreatic secretory trypsin inhibitor|serine protease inhibitor, Kazal type 1|tumor-associated trypsin inhibitor*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on SPINK1, check out the SPINK1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for SPINK1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
EK1241 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Serine protease inhibitor Kazal type 1 (SPINK1) drives proliferation and anoikis resistance in a subset of ovarian cancers
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