Human SPINK1/TATI ELISA Kit PicoKine™

Human SPINK1/TATI ELISA Kit PicoKine™ (96 Tests). Quantitate Human SPINK1 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 10pg/ml. Cited in 1 publication(s).

Product Info Summary

SKU: EK1241
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin).

Product Name

Human SPINK1/TATI ELISA Kit PicoKine™

See all SPINK1 products

SKU/Catalog Number

EK1241

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human SPINK1/TATI ELISA Kit PicoKine™ (96 Tests). Quantitate Human SPINK1 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human SPINK1/TATI ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1241)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: D24-C79

Sensitivity

<10 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1241 is reactive to SPINK1 in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin).

Application Guarantee

EK1241 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK1241-CAP Anti-Human SPINK1 Pre-coated 96-well strip microplate 1
EK1241-ST Human SPINK1 Standard 2 vials, 10 ng/tube
EK1241-DA Human SPINK1 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631262512502500500010000
O.D.0.0680.1010.1490.2600.3840.8671.4111.892

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect SPINK1, Dilution ratio of 1:1, concentration in serum is around 2ng/ml.

Some articles we found to cite concentrations of SPINK1 in samples: 24254562 (Pubmed IDs).

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human SPINK1/TATI ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)2451862581725417846136
Standard deviation16.9137.22418.8217.78148.07468.33
CV (%)6.9%7.3%7.2%7%8.3%7.6%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of SPINK1 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 12452382392252367.293%
Sample 218621592183118321779108.826.1%
Sample 358175596582359185788118.142%
*number of samples for each test n=16.

Gene/Protein Information For SPINK1 (Source: Uniprot.Org, NCBI)

Uniprot ID

P00995

Gene ID

6690

Gene Name

SPINK1

Full Name

Serine protease inhibitor Kazal-type 1

Weight

8.507kDa

Alternative Names

pancreatic secretory trypsin inhibitor; PCTT; PCTTSpink3; PSTI; PSTISerine protease inhibitor Kazal-type 1; serine peptidase inhibitor, Kazal type 1; serine protease inhibitor, Kazal type 1; SPINK1; Spink3; TATI; TATITumor-associated trypsin inhibitor SPINK1 PCTT, PSTI, Spink3, TATI, TCP serine peptidase inhibitor Kazal type 1 serine protease inhibitor Kazal-type 1|pancreatic secretory trypsin inhibitor|serine protease inhibitor, Kazal type 1|tumor-associated trypsin inhibitor

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on SPINK1, check out the SPINK1 Infographic

SPINK1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for SPINK1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK1241 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Serine protease inhibitor Kazal type 1 (SPINK1) drives proliferation and anoikis resistance in a subset of ovarian cancers

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7 Customer Q&As for Human SPINK1/TATI ELISA Kit PicoKine™

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

C. Hill

Verified customer

Asked: 2020-10-10

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2020-10-10

Question

Q: how can I thaw whole blood sample for SPINK1 ELISA after freezing?

P. King

Verified customer

Asked: 2019-12-12

Answer

A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test SPINK1 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.

Boster Scientific Support

Answered: 2019-12-12

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

S. Brown

Verified customer

Asked: 2019-02-22

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2019-02-22

Question

Q: can I use citrate plasma as samples in Human SPINK1 Picokine® ELISA Kit (Catalog # EK1241)?

W. Baker

Verified customer

Asked: 2019-02-13

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of SPINK1 causing disruption of its protein structure. SPINK1 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the SPINK1 ELISA, treating samples with various anticoagulants and decided that heparin can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2019-02-13

Question

Q: we need your recommendation regarding the dilution ratio of serum samples for detection of SPINK1 in Human plasma? I am trying to measure a few parameters and it requires 100ul of diluted samples for each well. We have low serum quantitys so we like to dilute as much as possible.

Verified Customer

Verified customer

Asked: 2018-10-31

Answer

A: without having an understanding the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the SPINK1 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2018-10-31

Question

Q: What is the optimal O.D. value for SPINK1 ELISA kit? I performed your SPINK1 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain SPINK1 even though the O.D. values are not very high?

Verified Customer

Verified customer

Asked: 2018-01-16

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the example mentioned, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this SPINK1 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of SPINK1 that can be declared/interpreted as positive by the above standard is 10pg/ml.

Boster Scientific Support

Answered: 2018-01-16

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification SPINK1 using the EK1241 Human SPINK1 Picokine® ELISA Kit?

L. Thompson

Verified customer

Asked: 2016-01-26

Answer

A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples to avoid protein degradation.

Boster Scientific Support

Answered: 2016-01-26

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