Human TGFBR2 / Tgf Beta Receptor Ii / TGF beta R2 ELISA Kit PicoKine™

Human TGFBR2 / Tgf Beta Receptor Ii / TGF beta R2 ELISA Kit PicoKine™ (96 Tests). Quantitate Human TGFBR2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Product Info Summary

SKU: EK1307
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Human TGFBR2 / Tgf Beta Receptor Ii / TGF beta R2 ELISA Kit PicoKine™

See all TGF-beta RII products

SKU/Catalog Number

EK1307

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human TGFBR2 / Tgf Beta Receptor Ii / TGF beta R2 ELISA Kit PicoKine™ (96 Tests). Quantitate Human TGFBR2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human TGFBR2 / Tgf Beta Receptor Ii / TGF beta R2 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1307)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: I24-D159

Sensitivity

<10 pg/ml

Assay Range

15.6 pg/ml - 1,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1307 is reactive to TGFBR2 in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK1307 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK1307-CAP Anti-Human TGFBR2 Pre-coated 96-well strip microplate 1
EK1307-ST Human TGFBR2 Standard 2 vials, 10 ng/tube
EK1307-DA Human TGFBR2 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015.631.262.51252505001000
O.D.0.0100.0770.2210.2210.4190.9971.6202.394

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect TGF-beta RII, Dilution ratio of 1:4, concentration in serum is around 600pg/ml.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human TGFBR2 / Tgf Beta Receptor Ii / TGF beta R2 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)3317942130166392
Standard deviation2.347.7%318.12.318.321.16
CV (%)7.1%4.6%4.3%7.7%5%5.4%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of TGF-beta RII in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 133343336341.223.5%
Sample 21791771721601727.384.2%
Sample 342144345343843811.582.6%
*number of samples for each test n=16.

Gene/Protein Information For TGFBR2 (Source: Uniprot.Org, NCBI)

Uniprot ID

P37173

Gene ID

7048

Gene Name

TGFBR2

Full Name

TGF-beta receptor type-2

Weight

64.568kDa

Superfamily

protein kinase superfamily

Alternative Names

AAT3; EC 2.7.11; EC 2.7.11.30; FAA3; HNPCC6; LDS1B; LDS2B; MFS2; RIIC; TAAD2; tbetaR-II; TGF-beta receptor type II; TGF-beta receptor type IIB; TGF-beta receptor type-2; TGF-beta RII; TGF-beta type II receptor; TGFbetaRII; TGFbeta-RII; TGFBR2; TGF-bRII; TGFR-2; transforming growth factor beta receptor type IIC; transforming growth factor, beta receptor II (70/80kDa) isoform 1; transforming growth factor, beta receptor II (70/80kDa) isoform 2; transforming growth factor, beta receptor II (70/80kDa); transforming growth factor, beta receptor II (70-80kD); Transforming growth factor-beta receptor TGFBR2 AAT3, FAA3, LDS1B, LDS2, LDS2B, MFS2, RIIC, TAAD2, TBR-ii, TBRII, TGFR-2, TGFbeta-RII transforming growth factor beta receptor 2 TGF-beta receptor type-2|TGF-beta receptor type IIB|TGF-beta type II receptor|tbetaR-II|transforming growth factor beta receptor II|transforming growth factor beta receptor type IIC|transforming growth factor, beta receptor II (70/80kDa)|transforming growth factor, beta receptor II alpha|transforming growth factor, beta receptor II beta|transforming growth factor, beta receptor II delta|transforming growth factor, beta receptor II epsilon|transforming growth factor, beta receptor II gamma

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on TGFBR2, check out the TGFBR2 Infographic

TGFBR2 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for TGFBR2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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18 Customer Q&As for Human TGFBR2 / Tgf Beta Receptor Ii / TGF beta R2 ELISA Kit PicoKine™

Question

Can you advise whether the capture and detection antibodies host is monoclonal or polyclonal?

Verified customer

Asked: 2020-09-24

Answer

For the Human TGFBR2 / Tgf Beta Receptor Ii / TGF Beta R2 ELISA Kit PicoKine™ (EK1307), the capture and detection antibodies are both goat polyclonal antibodies.

Boster Scientific Support

Answered: 2020-09-24

Question

What is the Expression host of the immunogen for the capture antibody of EK1307?

Verified customer

Asked: 2020-09-23

Answer

The Expression host of the immunogen for the capture antibody of the Human TGFBR2 / Tgf Beta Receptor Ii / TGF Beta R2 ELISA Kit PicoKine™ (EK1307) is goat.

Boster Scientific Support

Answered: 2020-09-23

Question

Is the Immunogen for the capture antibody of EK1307 native or recombinant?

Verified customer

Asked: 2020-09-23

Answer

The Immunogen for the capture antibody of the Human TGFBR2 / Tgf Beta Receptor Ii / TGF Beta R2 ELISA Kit PicoKine™ (EK1307) is recombinant.

Boster Scientific Support

Answered: 2020-09-23

Question

Is the Immunogen for the capture antibody of EK1307 full-length or partial?

Verified customer

Asked: 2020-09-23

Answer

The Immunogen for the capture antibody of the Human TGFBR2 / Tgf Beta Receptor Ii / TGF Beta R2 ELISA Kit PicoKine™ (EK1307) is partial.

Boster Scientific Support

Answered: 2020-09-23

Question

What animal species would the immunogen sequence for the capture antibody of EK1307 comes from?

Verified customer

Asked: 2020-09-23

Answer

The animal species the immunogen sequence for the capture antibody of the Human TGFBR2 / Tgf Beta Receptor Ii / TGF Beta R2 ELISA Kit PicoKine™ (EK1307) is Human.

Boster Scientific Support

Answered: 2020-09-23

Question

What animal species would the immunogen sequence for the detection antibody of EK1307 comes from?

Verified customer

Asked: 2020-09-23

Answer

The animal species the immunogen sequence for the detection antibody of the Human TGFBR2 / Tgf Beta Receptor Ii / TGF Beta R2 ELISA Kit PicoKine™ (EK1307) is Human.

Boster Scientific Support

Answered: 2020-09-23

Question

What is the Expression host of the immunogen for the detection antibody of EK1307?

Verified customer

Asked: 2020-09-23

Answer

The Expression host of the immunogen for the detection antibody of the Human TGFBR2 / Tgf Beta Receptor Ii / TGF Beta R2 ELISA Kit PicoKine™ (EK1307) is goat.

Boster Scientific Support

Answered: 2020-09-23

Question

Is the Immunogen for the detection antibody of EK1307 native or recombinant?

Verified customer

Asked: 2020-09-23

Answer

The Immunogen for the detection antibody of the Human TGFBR2 / Tgf Beta Receptor Ii / TGF Beta R2 ELISA Kit PicoKine™ (EK1307) is recombinant.

Boster Scientific Support

Answered: 2020-09-23

Question

Is the Immunogen for the detection antibody of EK1307 full-length or partial?

Verified customer

Asked: 2020-09-23

Answer

The Immunogen for the detection antibody of the Human TGFBR2 / Tgf Beta Receptor Ii / TGF Beta R2 ELISA Kit PicoKine™ (EK1307) is partial.

Boster Scientific Support

Answered: 2020-09-23

Question

What animal species for the standard immunogen sequence of EK1307 comes from?

Verified customer

Asked: 2020-09-23

Answer

The animal species for the standard immunogen sequence of the Human TGFBR2 / Tgf Beta Receptor Ii / TGF Beta R2 ELISA Kit PicoKine™ (EK1307) is Human?

Boster Scientific Support

Answered: 2020-09-23

Question

What is the Expression host for the standard immunogen sequence of EK1307?

Verified customer

Asked: 2020-09-23

Answer

The Expression host for the standard immunogen sequence of the Human TGFBR2 / Tgf Beta Receptor Ii / TGF Beta R2 ELISA Kit PicoKine™ (EK1307) is NSO.

Boster Scientific Support

Answered: 2020-09-23

Question

Is the Immunogen standard of EK1307 native or recombinant?

Verified customer

Asked: 2020-09-23

Answer

The Immunogen standard of the Human TGFBR2 / Tgf Beta Receptor Ii / TGF Beta R2 ELISA Kit PicoKine™ (EK1307) is recombinant.

Boster Scientific Support

Answered: 2020-09-23

Question

Is the Immunogen standard of EK1307 full-length or partial?

Verified customer

Asked: 2020-09-23

Answer

The Immunogen standard of the Human TGFBR2 / Tgf Beta Receptor Ii / TGF Beta R2 ELISA Kit PicoKine™ (EK1307) is partial.

Boster Scientific Support

Answered: 2020-09-23

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

Verified Customer

Verified customer

Asked: 2020-09-06

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2020-09-06

Question

Q: can I use citrate plasma as samples in Human TGF-beta RII Picokine® ELISA Kit (Catalog # EK1307)?

K. Wilson

Verified customer

Asked: 2020-06-28

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of TGF-beta RII causing degradation of its protein structure. TGF-beta RII may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the TGF-beta RII ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2020-06-28

Question

Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement TGF-beta RII using the EK1307 Human TGF-beta RII Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2018-09-12

Answer

A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples in order to prevent protein degradation.

Boster Scientific Support

Answered: 2018-09-12

Question

Q: which procedure should I follow in order to thaw whole blood sample for TGF-beta RII ELISA after freezing?

C. Harris

Verified customer

Asked: 2018-07-11

Answer

A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test TGF-beta RII for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2018-07-11

Question

Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2016-01-24

Answer

A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer may affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2016-01-24

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