Human Thrombomodulin ELISA Kit PicoKine™
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Thrombomodulin. 96wells/kit, with removable strips. Cited in 1 publication(s).
Human Thrombomodulin ELISA Kit PicoKine™ Info At A Glance
|Size:||96wells/kit, with removable strips.|
|Sample Type:||cell culture supernates, serum, plasma(heparin, EDTA, citrate) and urine.|
|Sample Volume:||100μl per well|
|Product Name||Human Thrombomodulin ELISA Kit PicoKine™
See all Thrombomodulin/BDCA-3 primary antibodies, ELISA kits and proteins
|Storage & Handling||Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)|
|Description||Detect Human Thrombomodulin/THBD with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, serum, plasma(heparin, EDTA, citrate) and urine. This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Thrombomodulin/THBD tissue specificity: Endothelial cells are unique in synthesizing thrombomodulin.|
|Cite This Product||Human Thrombomodulin ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0917)|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab.
|Sample Type||cell culture supernates, serum, plasma(heparin, EDTA, citrate) and urine.|
|Immunogen/Standard||Expression system for standard: NSO; Immunogen sequence: A19-S515|
|Antibody Clonalities||Capture antibody|Detection antibody
monoclonal antibody from mouse|polyclonal antibody from goat
|Cross Reactivity||There is no detectable cross-reactivity with other relevant proteins.|
|Pack Size||96wells/kit, with removable strips.|
Gene/Protein Basic Information For THBD (Source: Uniprot.org, NCBI)
|NCBI Gene Id||7056|
|Species Of This Entry||Human|
|Alternative Names||Thrombomodulin/BDCA-3|BDCA3; BDCA-3; CD141 antigen; CD141; Fetomodulin; THBD; THRM; Thrombomodulin; TM|
|Post Tranlational Modifications||Methylation; Demethylation; Cleavage; Phosphorylation; Deacetylation|
|Subcellular Localization||Membrane; Single-pass type I membrane protein.|
|Gene Location||20p11.21, on Chromasome 20, gene sequence: NC_000020.11 (23045633..23049672, complement)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For THBD (Source: Uniprot.org, NCBI)
|Protein Function||Thrombomodulin is a specific endothelial cell receptor that forms a 1:1 stoichiometric complex with thrombin. This complex is responsible for the conversion of protein C to the activated protein C (protein Ca). Once evolved, protein Ca scissions the activated cofactors of the coagulation mechanism, factor Va and factor VIIIa, and thereby reduces the amount of thrombin generated.|
|Tissue Specificity||Endothelial cells are unique in synthesizing thrombomodulin.|
|Related Diseases||Malignant Neoplasms; Inflammation; Thrombosis; Neoplasms; Disseminated Intravascular Coagulation; Tissue Adhesions; Hemolytic-uremic Syndrome; Von Willebrand Disease; Atherosclerosis; Coronary Artery Disease; Carcinoma; Uremia Syndrome; Cerebrovascular Accident; Kidney Failure; Hemorrhage; Microangiopathic Hemolytic Anemia; Anemia; Thrombophilia; Blood Coagulation Disorders; Anemia, Hemolytic|
|Related Pathways||Coagulation; Pathogenesis; Methylation; Blood Coagulation; Dna Methylation; Inflammatory Response; Hemostasis; Angiogenesis; Fibrinolysis; Cell Activation; Complement Activation; Transdifferentiation; Cell Proliferation; Localization; Demethylation; Cell Adhesion; Gene Silencing; Dna Hypermethylation; Tube Formation; Cell Cycle Arrest|
|Background||Thrombomodulin(TM), CD141 or BDCA-3 is an integral membrane protein expressed on the surface of endothelial cells and serves as a cofactor for thrombin. It reduces blood coagulation by converting thrombin to an anticoagulant enzyme from a procoagulant enzyme. Thrombomodulin is also expressed on human mesothelial cell, monocyte and a dendritic cell subset. In humans, thrombomodulin is encoded by the THBD gene. The protein has a molecular mass of 74kDa, and consists of a single chain with 5 distinct domains. Thrombomodulin functions as a cofactor in the thrombin-induced activation of protein C in the anticoagulant pathway by forming a 1:1 stoichiometric complex with thrombin. This raises the speed of protein C activation thousandfold. Thrombomodulin-bound thrombin has procoagulant effect at the same time by inhibiting fibrinolysis by cleaving thrombin-activatable fibrinolysis inhibitor(TAFI,aka carboxypeptidase B2) into its active form. Thrombomodulin is a glycoprotein on the surface of endothelial cells that, in addition to binding thrombin, regulates C3b inactivation by factor I. Mutations in the thrombomodulin gene(THBD) have also been reported to be associated with atypical hemolytic-uremic syndrome(aHUS).|
|Scientific References||PMID: 2820710 by Suzuki K., et al. Structure and expression of human thrombomodulin, a thrombin receptor on endothelium acting as a cofactor for protein C activation.|
PMID: 2822087 by Wen D., et al. Human thrombomodulin: complete cDNA sequence and chromosome localization of the gene.
Kit Components And Assay QC Data Details Of Human Thrombomodulin ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
|EK0917-CAP||96-well plate precoated with anti-Human Thrombomodulin/BDCA-3 antibody||1|
|EK0917-ST||lyophilized recombinant Human Thrombomodulin/BDCA-3 standard||10ng/tube|
|EK0917-DA||biotinylated anti-Human Thrombomodulin/BDCA-3 antibody||130ul|
|AR1106-1||sample diluent buffer||30ml|
|AR1106-2||antibody diluent buffer||12ml|
|AR1106-3||ABC diluent buffer||12ml|
|AR1104||TMB color developing agent||10ml|
|AR1105||TMB stop solution||10ml|
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EK0917 Human Thrombomodulin ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
We measured random samples of Human Thrombomodulin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We measured the performance consistency of Human Thrombomodulin ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
|Lots||Lot1 (pg/ml)||Lot2 (pg/ml)||Lot3 (pg/ml)||Lot4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
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Total number of citations: 1
|Kupchak, B.R., Kazman, J.B., Vingren, J.L., Levitt, D.E., Lee, E.C., Williamson, K.H.,..., & Deuster, P.A. (2017). Blood Hemostatic Changes During an Ultraendurance Road Cycling Event in a Hot Environment. Wilderness & Environmental Medicine. Adva...||PubMed ID 28739377|
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