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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).|
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Human VCAM-1 ELISA Kit PicoKine™
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human VCAM-1 ELISA Kit PicoKine™ (96 Tests). Quantitate Human VCAM1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Cite This Product
Human VCAM-1 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0537)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: F25-E698
156 pg/ml - 10,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK0537 is reactive to VCAM1 in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
EK0537 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
|EK0537-CAP||Anti-Human VCAM1 Pre-coated 96-well strip microplate||1|
|EK0537-ST||Human VCAM1 Standard||2 vials, 10 ng/tube|
|EK0537-DA||Human VCAM1 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human VCAM-1 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect VCAM-1/CD106, Dilution ratio of 1:300, concentration in serum is around 500ng/ml.
Some articles we found to cite concentrations of VCAM-1/CD106 in samples: 19236760 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human VCAM-1 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of VCAM-1/CD106 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For VCAM1 (Source: Uniprot.Org, NCBI)
Vascular cell adhesion protein 1
CD106 antigen; CD106; DKFZp779G2333; INCAM-100; L1CAM; MGC99561; vascular cell adhesion molecule 1; vascular cell adhesion protein 1; V-CAM 1; VCAM1; VCAM-1 VCAM1 CD106, INCAM-100 vascular cell adhesion molecule 1 vascular cell adhesion protein 1|CD106 antigen*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on VCAM1, check out the VCAM1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for VCAM1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
EK0537 has been cited in 19 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Resistance exercise: a strategy to attenuate inflammation and protein-energy wasting in hemodialysis patients?
Protective Role of Antioxidant Huskless Barley Extracts on TNF-?-Induced Endothelial Dysfunction in Human Vascular Endothelial Cells
Signaling via the CXCR5/ERK pathway is mediated by CXCL13 in mice with breast cancer
Metoprolol Improves Endothelial Function in Patients with Cardiac Syndrome X
Evaluation of the effect of Vaccinium arctostaphylos L fruit extract on serum inflammatory biomarkers in adult hyperlipidemic patients: a randomized double-blind placebo-controlled clinical trial
Neurally mediated syncope: Is it really an endothelial dysfunction?
Endothelial dysfunction state in migraine headache and neutrally mediated syncope in children and young adults
FGF-23/Vitamin D Axis in Type 1 Diabetes: The Potential Role of Mineral Metabolism in Arterial Stiffness
Sun HJ, Chen D, Wang PY, Wan MY, Zhang CX, Zhang ZX, Lin W, Zhang F. Oxid Med Cell Longev. 2017;2017:6905217. doi: 10.1155/2017/6905217. Epub 2017 Nov 19. Salusin-β Is Involved in Diabetes Mellitus-Induced Endothelial Dysfunction via Degradation o...
Maggi P, Bruno G, Perilli F, Saracino A, Volpe A, Santoro C, Ladisa N, Angarano G. In Vivo. 2017 Jan 2;31(1):125-131. Effects of Therapy with Maraviroc on the Carotid Intima Media Thickness in HIV-1/HCV Co-infected Patients
Denkovskij, J., Bagdonas, E., Kusleviciute, I., Mackiewicz, Z., Unguryte, A., Porvaneckas, N.,..., & Bernotiene, E. (2017). Paracrine Potential of the Human Adipose Tissue-Derived Stem Cells to Modulate Balance between Matrix Metalloproteinases an...
Sun H, Zhu X, Cai W, Qiu L. Sun H1, Zhu X2, Cai W3, Qiu L4. Hypaphorine Attenuates Lipopolysaccharide-Induced Endothelial Inflammation via Regulation of TLR4 and PPAR-γ Dependent on PI3K/Akt/mTOR Signal Pathway.
Zhang X, Zheng Z, Shu B, Liu X, Zhang Z, Liu Y, Bai B, Hu Q, Mao P, Wang H. J Virol. 2013 Nov;87(22):12407-21. Doi: 10.1128/Jvi.02090-13. Epub 2013 Sep 11. Human Astrocytic Cells Support Persistent Coxsackievirus B3 Infection.
Asgary S, Keshvari M, Sahebkar A, Hashemi M, Rafieian-Kopaei M. Arya Atheroscler. 2013 Nov;9(6):326-31. Clinical Investigation Of The Acute Effects Of Pomegranate Juice On Blood Pressure And Endothelial Function In Hypertensive Individuals.
Asgary S, Keshvari M, Afshani Mr, Amiri M, Laher I, Javanmard Sh. Isrn Nutr. 2014 Mar 4;2014:405867. Doi: 10.1155/2014/405867. Ecollection 2014. Effect Of Fresh Orange Juice Intake On Physiological Characteristics In Healthy Volunteers.
Zhang R, Jiang F, Chen Cs, Wang T, Feng J, Tao T, Qin X. Mediators Inflamm. 2015;2015:956082. Doi: 10.1155/2015/956082. Epub 2015 May 31. Serum Levels Of Il-1 ?? , Il-6, Tgf- ?? , And Mmp-9 In Patients Undergoing Carotid Artery Stenting And Regula...
Asgary S, Kelishadi R, Rafieian-Kopaei M, Najafi S, Najafi M, Sahebkar A. Pediatr Cardiol. 2013 Oct;34(7):1729-35. Doi: 10.1007/S00246-013-0693-5. Epub 2013 Apr 27. Investigation Of The Lipid-Modifying And Antiinflammatory Effects Of Cornus Mas L....
Barros Af, Borges Na, Ferreira Dc, Carmo Fl, Rosado As, Fouque D, Mafra D. Future Microbiol. 2015;10(4):517-26. Doi: 10.2217/Fmb.14.140. Is There Interaction Between Gut Microbial Profile And Cardiovascular Risk In Chronic Kidney Disease Patients?
Huang Wy, Wang J, Liu Ym, Zheng Qs, Li Cy. Eur J Pharmacol. 2014 Jan 15;723:67-72. Doi: 10.1016/J.Ejphar.2013.11.041. Epub 2013 Dec 11. Inhibitory Effect Of Malvidin On Tnf-??-Induced Inflammatory Response In Endothelial Cells.
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