Human VWF-A2/VWF ELISA Kit PicoKine™

Human VWF-A2/VWF ELISA Kit PicoKine™ (96 Tests). Quantitate Human VWF in cell culture supernatants, serum and plasma (heparin, EDTA, citrate ). Sensitivity: 50pg/ml. Cited in 1 publication(s).

Product Info Summary

SKU: EK1743
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA, citrate ).

Product Name

Human VWF-A2/VWF ELISA Kit PicoKine™

See all VWF products

SKU/Catalog Number

EK1743

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human VWF-A2/VWF ELISA Kit PicoKine™ (96 Tests). Quantitate Human VWF in cell culture supernatants, serum and plasma (heparin, EDTA, citrate ). Sensitivity: 50pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human VWF-A2/VWF ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1743)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: D1498-V1665

Sensitivity

<50 pg/ml

Assay Range

312 pg/ml - 20,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1743 is reactive to VWF in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA, citrate ).

Application Guarantee

EK1743 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK1743-CAP Anti-Human VWF Pre-coated 96-well strip microplate 1
EK1743-ST Human VWF Standard 2 vials, 20 ng/tube
EK1743-DA Human VWF Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)03126251250250050001000020000
O.D.0.0370.1800.3060.5111.0131.4482.0892.531

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect VWF, Dilution ratio of 1:5, concentration in serum is around 12ng/ml.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human VWF-A2/VWF ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)5711728976260019879834
Standard deviation35.473.3497.8640.2147.04717.9
CV (%)6.2%4.3%5.1%6.7%7.4%7.3%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of VWF in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 157155261458658022.663.9%
Sample 21728165916121716167846.522.7%
Sample 397629879885581359157711.167.7%
*number of samples for each test n=16.

Gene/Protein Information For VWF (Source: Uniprot.Org, NCBI)

Uniprot ID

P04275

Gene ID

7450

Gene Name

VWF

Full Name

von Willebrand factor

Weight

309.265kDa

Alternative Names

coagulation factor VIII VWF; F8; F8VWF; von Willebrand factor; VWD; vWF VWF F8VWF, VWD von Willebrand factor von Willebrand factor|coagulation factor VIII VWF

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on VWF, check out the VWF Infographic

VWF infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for VWF: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK1743 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Yang N, Qin S, Wang M, Chen B, Yuan N, Fang Y, Yao S, Jiao P, Yu Y, Zhang Y, Wang J. Cytotechnology. 2013 Aug;65(4):541-51. Doi: 10.1007/S10616-012-9502-X. Epub 2012 Nov 22. Pinocembrin, A Major Flavonoid In Propolis, Improves The Biological Funct...

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7 Customer Q&As for Human VWF-A2/VWF ELISA Kit PicoKine™

Question

Do you have information on the antibodies of EK1743 where they bind?

Verified customer

Asked: 2020-11-24

Answer

The immunogen sequence of antibody pairs in the Human VWF-A2 ELISA Kit PicoKine™ (EK1743) is D1498-V1665.

Boster Scientific Support

Answered: 2020-11-25

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

R. Martinez

Verified customer

Asked: 2020-09-26

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2020-09-26

Question

What is the isotype of the monoclonal antibody used as the capture antibody in the EK1743 kit?

Verified customer

Asked: 2019-10-01

Answer

The capture antibody and detection antibody in the Human VWF-A2 ELISA Kit PicoKine EK1743 kit are polyclonal antibody from sheep.

Boster Scientific Support

Answered: 2019-10-08

Question

Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification VWF using the EK1743 Human VWF Picokine® ELISA Kit?

C. Bhasin

Verified customer

Asked: 2019-07-29

Answer

A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples to limit protein degradation.

Boster Scientific Support

Answered: 2019-07-29

Question

Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?

E. Lee

Verified customer

Asked: 2018-06-21

Answer

A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can have an affect on immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2018-06-21

Question

Q: Can VWF ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

P. Anand

Verified customer

Asked: 2017-10-16

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2017-10-16

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2017-08-13

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2017-08-13

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