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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum and plasma (heparin, EDTA, citrate).|
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Mouse CD39/ENTPD1 ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Mouse CD39/ENTPD1 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Entpd1 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse CD39/ENTPD1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2013)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: T38-I478
31.2 pg/ml - 2,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK2013 is reactive to Entpd1 in Mouse samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
EK2013 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of CD39/ENTPD1
Ectonucleoside triphosphate diphosphohydrolase-1 (gene: ENTPD1; protein: NTPDase1) also known as CD39 (Cluster of Differentiation 39), is a typical cell surface-located enzymes with an extracellularly facing catalytic site. This gene is mapped to 19 C3; 19 34.25 cM. NTPDase1 is an ectonucleotidase that catalyse the hydrolysis of γ- and β-phosphate residues of triphospho- and diphosphonucleosides to the monophosphonucleoside derivative. For example, NTPDase1 hydrolyzes P2 receptor ligands, namely ATP, ADP, UTP and UDP with similar efficacy. NTPDase1 can therefore affect P2 receptor activation and functions.
|EK2013-CAP||Anti-Mouse Entpd1 Pre-coated 96-well strip microplate||1|
|EK2013-ST||Mouse Entpd1 Standard||2 vials, 10 ng/tube|
|EK2013-DA||Mouse Entpd1 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Mouse CD39/ENTPD1 PicoKine ELISA Kit Standard Curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect CD39/ENTPD1, Dilution ratio of 1:1, concentration in serum is 800-2500pg/ml.
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse CD39/ENTPD1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of CD39/ENTPD1 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For Entpd1 (Source: Uniprot.Org, NCBI)
Ectonucleoside triphosphate diphosphohydrolase 1
GDA1/CD39 NTPase family
ADPase; ATPDase; ATP-diphosphatase; CD39 antigen; CD39; CD39EC 188.8.131.52; DKFZp686D194; DKFZp686I093; EC 3.6.1; ecto-apyrase; Ecto-ATP diphosphohydrolase 1; Ecto-ATPase 1; Ecto-ATPDase 1; ectonucleoside triphosphate diphosphohydrolase 1; ENTPD1; FLJ40921; FLJ40959; Lymphoid cell activation antigen; NTPD1; NTPDase 1; NTPDase-1 Entpd1|2610206B08Rik, AA408691, Cd39, E130009M23Rik, NTPDa, NTPDase-1, ectoa|ectonucleoside triphosphate diphosphohydrolase 1|ectonucleoside triphosphate diphosphohydrolase 1|CD39 antigen|NTPDase 1|ecto-ATP diphosphohydrolase 1|ecto-ATPDase 1|ecto-ATPase 1|ectoapyrase|lymphoid cell activation antigen*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Entpd1, check out the Entpd1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Entpd1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
No publications found for EK2013
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