ENTPD1 Antibodies

Ectonucleoside triphosphate diphosphohydrolase 1 (ENTPD1)

In the nervous system, could hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. Could also be implicated in the prevention of platelet aggregation by hydrolyzing platelet-activating ADP to AMP.

Hydrolyzes ATP and ADP equally well. .

Gene Information

Human
Gene Name:	ENTPD1
Uniprot:	P49961
Entrez:	953

Family

Belongs to:
GDA1/CD39 NTPase family

Alternative Names

ADPase; ATPDase; ATP-diphosphatase; CD39 antigen; CD39; CD39EC 3.6.1.5; DKFZp686D194; DKFZp686I093; EC 3.6.1; ecto-apyrase; Ecto-ATP diphosphohydrolase 1; Ecto-ATPase 1; Ecto-ATPDase 1; ectonucleoside triphosphate diphosphohydrolase 1; ENTPD1; FLJ40921; FLJ40959; Lymphoid cell activation antigen; NTPD1; NTPDase 1; NTPDase-1

Molecular Weight

Mass (kDA):
57.965 kDA

Genomic Context

Human
Location:	10q24.1
Sequence:	10; NC_000010.11 (95710901..95877266)

Tissue Specificity

Expressed primarily on activated lymphoid cells. Also expressed in endothelial tissues. Isoform 1 and isoform 3 are present in both placenta and umbilical vein, whereas isoform 2 is present in placenta only.

Subcellular Localizations

Membrane; Multi-pass membrane protein.

Diseases

• Neoplasms
• Inflammation
• Tumor Angiogenesis
• Malignant Neoplasms
• Diabetes Mellitus
• Melanoma, Experimental
• Decreased Immunologic Activity [pe]
• Vascular System Injuries
• Tissue Adhesions
• Thrombosis
• Neoplasm Metastasis
• Malnutrition

• Diabetic Nephropathy
• Melanoma
• Ischemia
• Chronic Inflammation
• Malignant Paraganglionic Neoplasm
• Diabetes Mellitus, Non-insulin-dependent
• Insulin Resistance

Interacting Proteins

• RANBP9

Pathways

• Angiogenesis
• Cell Proliferation
• Endothelial Cell Proliferation
• Glucose Homeostasis
• Cell Death
• Cell Adhesion
• Platelet Activation
• Regeneration
• Reverse Transcription
• Receptor Internalization
• Response To Atp

• Cell Growth
• Liver Regeneration
• Vasoconstriction
• Pathogenesis
• Exocytosis
• Autophagy

PTMs

• Phosphorylation

• Dephosphorylation

Research Areas

• Lipid and Metabolism

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/ENTPD1

Boster Bio - The Best Uses Of The ENTPD1 Marker

This article discusses the ENTPD1 marker and its uses in molecular biology. It provides information about its validation, application, and optimization. You can download the validation images and procedure for free. This guide will help you choose the best ELISA kit for your needs. There are many advantages of using an ELISA kit for molecular biology. We'll look at three of them:

ENTPD1 Marker

ENTPD1, also known as NTPDase-1 or CD39, is a protein found on the surface of most cells, including B lymphocytes. It hydrolyzes beta-phosphate residues in nucleotides and prefers ATP as its substrate. Among its functions, ENTPD1 is involved in purinergic signaling and may have therapeutic implications for vascular diseases.

Validation

The ENTPD1 gene is responsible for the hydrolysis of extracellular purine NTP. These molecules are toxic to human cells and may be used in cancer diagnosis. The gene's expression profile has been analyzed and mapped onto the murine atlas. The expression of the genes that define cytotoxic and effector memory T cells is consistent with the results obtained with ProjecTILs. The gene's expression was also enriched in tumor-infiltrating lymphocytes (TILs) from patients with melanoma.

Application

The ENTPD1 marker has several functional associations with other genes, including IL-6, IL-33, and CCR7. These molecules are associated with various interleukin and chemokine signaling genes. They are also associated with TCR repertoire, which postulates a role in modulating these pathways. The present study used this marker to identify the effect of COVID-19 on the TCR repertoire and the expression of ENTPD1 and CD39 in COVID-19 patients.

The ENTPD1 marker can be used in the detection of cancer cells, inflammatory agents, and various developmental diseases. Boster Bio antibody kits are highly sensitive, with sensitivity reaching picogram levels. They can be purchased from tebu-bio. These antibodies are made from purified human serum. Boster Bio provides a wide range of immunological reagents for various applications, including IHC, WB, and Flow.

The ENTPD1 gene, which belongs to the family of entp-like proteins, plays a pivotal role in the immune system. Polymorphisms in its promoter region are associated with low levels of mRNA. The ENTPD1 gene has been found to be essential for the immune system and may even be a marker of disease severity. But its significance cannot be overstated.

The ENTPD1 gene is regulated by the endogenous antisense RNA (EST-1). The ENTPD1 RNA is a multiple splice variant of ENTPD1, which has a corresponding antisense position in the 10q24.1 region of the chromosome. The ENTPD1 RNA expression levels were pooled in severe and convalescent COVID-19 patients and in healthy controls. ENTPD1-AS gene expression levels were determined, and the genes associated with high and low levels were identified. The lines in each subgroup represented the individual gene sets, with upregulated genes appearing on the left side and downregulated genes appearing on the right.

Optimization

Optimizing the ENTPD1 marker is one of the most important steps in molecular biology. The methods for DNA manipulation and investigation are widely used in the scientific community. Boster bio provides users with extensive technical support, blogs, and disease information. In addition, its high-quality lysis buffers ensure good results and lessen cross-linking intensity. This guide will help users optimize ENTPD1 expression levels in various biological conditions and achieve high-quality results.

Safety

A new drug candidate containing a soluble ecto-enzyme of ENTPD1 may have therapeutic potential in the treatment of cancers. It is able to inhibit the activity of ENTPD1, which might also have anticancer properties. However, its exact mechanism is not yet known. However, the drug may be safe for use in humans. Safety of the ENTPD1 marker depends on its utility in research and development.

ENTPD1 is a cellular enzyme that metabolizes ATP and ADP. The enzyme is expressed on many cells in the body and is found to be expressed on many types of cell surfaces. It is involved in thromboregulation, purinergic signaling, and hydrolysis of other nucleotides. The drug is designed to detect the activity of the ENTPD1 enzyme in a cell, including lymphocytes.