Mouse IL-5/Interleukin-5 ELISA Kit PicoKine®

IL-5 ELISA kit for Mouse

Mouse IL-5/Interleukin-5 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Il5 in cell culture supernatants and serum. Sensitivity: 2pg/ml. Cited in 24 publication(s).

Product Info Summary

SKU: EK0408
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants and serum.

Product Name

Mouse IL-5/Interleukin-5 ELISA Kit PicoKine®

View all IL-5 ELISA kits

SKU/Catalog Number

EK0408

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse IL-5/Interleukin-5 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Il5 in cell culture supernatants and serum. Sensitivity: 2pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Mouse IL-5/Interleukin-5 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0408)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: M21-G133

Sensitivity

<2 pg/ml

Assay Range

15.6 pg/ml - 1,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0408 is reactive to Il5 in Mouse samples

Validated Sample Types

cell culture supernatants and serum.

Application Guarantee

EK0408 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of IL-5

Interleukin-5 (IL-5) is also known as eosinophil differentiation factor (EDF). IL-5 is a potential candidate gene in the pathogenesis of asthma, as it is the main cytokine controlling eosinophil activity and eosinophils are pivotal in the development of airway inflammation. The predicted amino acid sequence of 134 amino acids is identical with that recently reported for human interleukin-5 but shows no significant homology with other known hemopoietic growth regulators. IL-5 is a lineage-specific hematopoietic growth factor that stimulates the production of eosinophils and eosinophil colonies from normal human bone marrow cells. IL-5 gene to human chromosome 5 at bands q23-31. Human and mouse of IL-5 share 70% amino acid sequence homology.

Kit Components

Catalog Number Description Quantity
EK0408-CAP Anti-Mouse Il5 Pre-coated 96-well strip microplate 1
EK0408-ST Mouse Il5 Standard 2 vials, 10 ng/tube
EK0408-DA Mouse Il5 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015.631.262.51252505001000
O.D.0.0250.0830.1440.2580.4670.8821.5542.052

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect IL-5, Dilution ratio of 1:1.

Some articles we found to cite concentrations of IL-5 in samples: 22166637 (Pubmed IDs).

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse IL-5/Interleukin-5 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)3622045334214478
Standard deviation2.529.2421.292.5810.730.59
CV (%)7%4.2%4.7%7.6%5%6.4%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of IL-5 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 136313735342.276.6%
Sample 222022826725624219.357.9%
Sample 345340239743442123.075.4%
*number of samples for each test n=16.

Gene/Protein Information For Il5 (Source: Uniprot.Org, NCBI)

Uniprot ID

P04401

Gene ID

16191

Gene Name

Il5

Full Name

Interleukin-5

Weight

15.41kDa

Superfamily

IL-5 family

Alternative Names

BCDF mu; B-cell differentiation factor I; BCGFII; EDF; Eo-CSF; Eosinophil differentiation factor; IL5; IL-5; IL-5T-cell replacing factor; interleukin 5 (colony-stimulating factor, eosinophil); interleukin-5; TRF; TRFB cell differentiation factor I Il5|Il, Il-5|interleukin 5|interleukin-5|B-cell growth factor II|BCGF-II|T-cell replacing factor|TRF|cytotoxic T-lymphocyte inducer|eosinophil differentiation factor

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Il5, check out the Il5 Infographic

Il5 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Il5: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0408 has been cited in 24 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Inhibitory effects of catalpol coordinated with budesonide and their relationship with cytokines and Interleukin-13 expression

NK Cells Alleviate Lung Inflammation by Negatively Regulating Group 2 Innate Lymphoid Cells

Knockdown of miR‑205‑5p alleviates the inflammatory response in allergic rhinitis by targeting B‑cell lymphoma 6

The AKT inhibitor MK2206 suppresses airway inflammation and the pro‑remodeling pathway in a TDI‑induced asthma mouse model

Immunomodulatory Effects of Phosphorylated Radix Cyathulae officinalis Polysaccharides in Immunosuppressed Mice

Molecular cloning of enolase from Trichinella spiralis and the protective immunity in mice

Immune potentials of the Mucor-fermented Mao-tofu and especially its soluble extracts for the normal mice

Pollen/TLR4 Innate Immunity Signaling Initiates IL-33/ST2/Th2 Pathways in Allergic Inflammation

Gentiopicroside ameliorates ovalbumin-induced airway inflammation in a mouse model of allergic asthma via regulating SIRT1/NF-κB signaling pathway

Systemic delivery of TNF-armed myxoma virus plus immune checkpoint inhibitor eliminates lung metastatic mouse osteosarcoma

Catalpol alleviates ovalbumin-induced asthma in mice: Reduced eosinophil infiltration in the lung

Fangxiao Formula alleviates airway inflammation and remodeling in rats with asthma via suppression of transforming growth factor-β/Smad3 signaling pathway

Effects of catalpol on bronchial asthma and its relationship with cytokines

Cui H,Cheng Y,He Y,Cheng W,Zhao W,Zhao H,Zhou FH,Wang L,Dong J,Cai S.The AKT inhibitor MK2206 suppresses airway inflammation and the pro‑remodeling pathway in a TDI‑induced asthma mouse model.Mol Med Rep.2020 Nov;22(5):3723-3734.doi:10.3892/mmr.2020.11450.Epub 2020 Aug 21.PMID:33000187;PMCID:PMC7533517.
Species: Mouse
EK0408 usage in article: APP:ELISA, SAMPLE:BALF, DILUTION:NA

Catalpol alleviates ovalbumin-induced asthma in mice: Reduced eosinophil infiltration in the lung

5-BDBD ameliorates an OVA-induced allergic asthma by the reduction of Th2 cytokines production

Exogenous interleukin-10 alleviates allergic inflammation but inhibits local interleukin-10 expression in a mouse allergic rhinitis model

Yang N, Zhang H, Cai X, Shang Y. Int J Mol Med. 2018 Feb;41(2):818-828. doi: 10.3892/ijmm.2017.3292. Epub 2017 Nov 28. Epigallocatechin-3-gallate inhibits inflammation and epithelial-mesenchymal transition through the PI3K/AKT pathway via upregula...

Han, X.P., Zhang, F.Q., Tan, X.S., Liu, L., Ma, W.X., Ou-Yang, H.F., & Wu, C.G. (2017). EPO modified MSCs can inhibit asthmatic airway remodeling in an animal model. Journal of Cellular Biochemistry. Advance online publication. doi: 10.1002/jcb.26268

Li J, Li L, Zuo H, Ke C, Yan B, Wen H, Zhang Y, Yang X. Plos One. 2014 Feb 3;9(2):E87887. Doi: 10.1371/Journal.Pone.0087887. Ecollection 2014. T-Helper Type-2 Contact Hypersensitivity Of Balb/C Mice Aggravated By Dibutyl Phthalate Via Long-Term De...

Wang Sb, Deng Yq, Ren J, Xiao Bk, Chen Z, Tao Zz. Scand J Immunol. 2013 Dec;78(6):507-15. Doi: 10.1111/Sji.12118. Lactoferrin Administration Into The Nostril Alleviates Murine Allergic Rhinitis And Its Mechanisms.

Chen Y, Duan Y, Kang Y, Yang X, Jiang M, Zhang L, Li G, Yin Z, Hu W, Dong P, Li X, Hajjar Dp, Han J. J Biol Chem. 2012 Dec 21;287(52):43340-50. Doi: 10.1074/Jbc.M112.403394. Epub 2012 Nov 13. Activation Of Liver X Receptor Induces Macrophage Inter...

Presti I, D'Orazio G, Labra M, La Ferla B, Mezzasalma V, Bizzaro G, Giardina S, Michelotti A, Tursi F, Vassallo M, Di Gennaro P. Appl Microbiol Biotechnol. 2015 Jul;99(13):5613-26. Doi: 10.1007/S00253-015-6482-8. Epub 2015 Mar 7. Evaluation Of The...

Zhu Hc, Qiu T, Liu Xh, Dong Wc, Weng Xd, Hu Ch, Kuang Yl, Gao Rh, Dan C, Tao T. Cell Immunol. 2012;274(1-2):12-8. Doi: 10.1016/J.Cellimm.2012.02.012. Epub 2012 Mar 14. Tolerogenic Dendritic Cells Generated By Relb Silencing Using Shrna Prevent Acu...

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11 Customer Q&As for Mouse IL-5/Interleukin-5 ELISA Kit PicoKine®

Question

Could you provide the expression host of the standard?

Verified customer

Asked: 2022-06-06

Answer

Hi, thank you for your question. The expression host of the standard is NS0, mouse myeloma cell line.

Boster Scientific Support

Answered: 2022-06-07

Question

Does EK0408 contain any recombinant proteins other than Standard?

Verified customer

Asked: 2022-05-03

Answer

The Mouse IL-5/Interleukin-5 ELISA Kit PicoKine® (EK0408) doesn't contain any recombinant proteins other than Standard.

Boster Scientific Support

Answered: 2022-05-16

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2020-10-30

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2020-10-30

Question

Q: how to proceed with the analysis of ELISA data? I have obtained IL-5 level in serum.

D. Huang

Verified customer

Asked: 2020-04-06

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2020-04-06

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification IL-5 using the EK0408 Mouse IL-5 Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2019-10-17

Answer

A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to prevent protein degradation.

Boster Scientific Support

Answered: 2019-10-17

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2019-08-16

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2019-08-16

Question

Q: can you recommend the dilution ratio of serum samples for detection of IL-5 in Mouse serum? I am trying to measure a a number of analytes and it requires 100ul of diluted samples for each well. We have low serum quantitys so we like to dilute as much as possible.

Verified Customer

Verified customer

Asked: 2019-06-10

Answer

A: unable to know the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the IL-5 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2019-06-10

Question

Q: Can IL-5 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

Verified Customer

Verified customer

Asked: 2018-05-08

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is needed. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2018-05-08

Question

Q: how to thaw whole blood sample for IL-5 ELISA after freezing?

Verified Customer

Verified customer

Asked: 2017-11-14

Answer

A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test IL-5 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2017-11-14

Question

Q: if the enzyme conjugated IL-5 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the IL-5 antigen?

Verified Customer

Verified customer

Asked: 2016-04-19

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2016-04-19

Question

Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2015-05-09

Answer

A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2015-05-09

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