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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants and serum.|
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Mouse IL-5/Interleukin-5 ELISA Kit PicoKine™
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Mouse IL-5/Interleukin-5 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Il5 in cell culture supernatants and serum. Sensitivity: 2pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Cite This Product
Mouse IL-5/Interleukin-5 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0408)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: sf21; Immunogen sequence: M21-G133
15.6 pg/ml - 1,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK0408 is reactive to Il5 in Mouse samples
Validated Sample Types
cell culture supernatants and serum.
EK0408 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
|EK0408-CAP||Anti-Mouse Il5 Pre-coated 96-well strip microplate||1|
|EK0408-ST||Mouse Il5 Standard||2 vials, 10 ng/tube|
|EK0408-DA||Mouse Il5 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Mouse IL-5 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect IL-5, Dilution ratio of 1:1.
Some articles we found to cite concentrations of IL-5 in samples: 22166637 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse IL-5/Interleukin-5 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of IL-5 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For Il5 (Source: Uniprot.Org, NCBI)
BCDF mu; B-cell differentiation factor I; BCGFII; EDF; Eo-CSF; Eosinophil differentiation factor; IL5; IL-5; IL-5T-cell replacing factor; interleukin 5 (colony-stimulating factor, eosinophil); interleukin-5; TRF; TRFB cell differentiation factor I Il5|Il, Il-5|interleukin 5|interleukin-5|B-cell growth factor II|BCGF-II|T-cell replacing factor|TRF|cytotoxic T-lymphocyte inducer|eosinophil differentiation factor*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Il5, check out the Il5 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Il5: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
EK0408 has been cited in 11 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Cui H,Cheng Y,He Y,Cheng W,Zhao W,Zhao H,Zhou FH,Wang L,Dong J,Cai S.The AKT inhibitor MK2206 suppresses airway inflammation and the pro‑remodeling pathway in a TDI‑induced asthma mouse model.Mol Med Rep.2020 Nov;22(5):3723-3734.doi:10.3892/mmr.2020.11450.Epub 2020 Aug 21.PMID:33000187;PMCID:PMC7533517.
EK0408 usage in article: APP:ELISA, SAMPLE:BALF, DILUTION:NA
Catalpol alleviates ovalbumin-induced asthma in mice: Reduced eosinophil infiltration in the lung
5-BDBD ameliorates an OVA-induced allergic asthma by the reduction of Th2 cytokines production
Exogenous interleukin-10 alleviates allergic inflammation but inhibits local interleukin-10 expression in a mouse allergic rhinitis model
Yang N, Zhang H, Cai X, Shang Y. Int J Mol Med. 2018 Feb;41(2):818-828. doi: 10.3892/ijmm.2017.3292. Epub 2017 Nov 28. Epigallocatechin-3-gallate inhibits inflammation and epithelial-mesenchymal transition through the PI3K/AKT pathway via upregula...
Han, X.P., Zhang, F.Q., Tan, X.S., Liu, L., Ma, W.X., Ou-Yang, H.F., & Wu, C.G. (2017). EPO modified MSCs can inhibit asthmatic airway remodeling in an animal model. Journal of Cellular Biochemistry. Advance online publication. doi: 10.1002/jcb.26268
Li J, Li L, Zuo H, Ke C, Yan B, Wen H, Zhang Y, Yang X. Plos One. 2014 Feb 3;9(2):E87887. Doi: 10.1371/Journal.Pone.0087887. Ecollection 2014. T-Helper Type-2 Contact Hypersensitivity Of Balb/C Mice Aggravated By Dibutyl Phthalate Via Long-Term De...
Wang Sb, Deng Yq, Ren J, Xiao Bk, Chen Z, Tao Zz. Scand J Immunol. 2013 Dec;78(6):507-15. Doi: 10.1111/Sji.12118. Lactoferrin Administration Into The Nostril Alleviates Murine Allergic Rhinitis And Its Mechanisms.
Chen Y, Duan Y, Kang Y, Yang X, Jiang M, Zhang L, Li G, Yin Z, Hu W, Dong P, Li X, Hajjar Dp, Han J. J Biol Chem. 2012 Dec 21;287(52):43340-50. Doi: 10.1074/Jbc.M112.403394. Epub 2012 Nov 13. Activation Of Liver X Receptor Induces Macrophage Inter...
Presti I, D'Orazio G, Labra M, La Ferla B, Mezzasalma V, Bizzaro G, Giardina S, Michelotti A, Tursi F, Vassallo M, Di Gennaro P. Appl Microbiol Biotechnol. 2015 Jul;99(13):5613-26. Doi: 10.1007/S00253-015-6482-8. Epub 2015 Mar 7. Evaluation Of The...
Zhu Hc, Qiu T, Liu Xh, Dong Wc, Weng Xd, Hu Ch, Kuang Yl, Gao Rh, Dan C, Tao T. Cell Immunol. 2012;274(1-2):12-8. Doi: 10.1016/J.Cellimm.2012.02.012. Epub 2012 Mar 14. Tolerogenic Dendritic Cells Generated By Relb Silencing Using Shrna Prevent Acu...
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