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Product Info Summary
| SKU: | EK0943 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Mouse MPO/Myeloperoxidase ELISA Kit PicoKine®
View all Myeloperoxidase/MPO ELISA kits
SKU/Catalog Number
EK0943
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse MPO/Myeloperoxidase ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Mpo in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse MPO/Myeloperoxidase ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0943)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: M16-T718
Sensitivity
<10 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK0943 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0943 is reactive to MPO in Mouse samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK0943 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Myeloperoxidase/MPO
Myeloperoxidase (MPO) is a mammalian phagocyte hemoprotein thought to primarily mediate host defense reactions. It is abundantly expressed in neutrophils and secreted during their activation. Myeloperoxidase is part of the host defense system of human polymorphonuclear leukocytes, responsible for microbicidal activity against a wide range of organisms. It is located in the nucleus as well as in the cytoplasm. Intranuclear MPO may help to protect DNA against damage resulting from oxygen radicals produced during myeloid cell maturation and function.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0943-CAP | Anti-Mouse MPO Pre-coated 96-well strip microplate | 1 |
| EK0943-ST | Mouse MPO Standard | 2 vials, 10 ng/tube |
| EK0943-DA | Mouse MPO Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.
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Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.034 | 0.070 | 0.108 | 0.184 | 0.427 | 0.660 | 1.378 | 2.095 |
Data Example Images
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Mouse MPO PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse MPO/Myeloperoxidase ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 354 | 1809 | 5328 | 362 | 1718 | 5171 |
| Standard deviation | 19.11 | 115.77 | 266.4 | 346.457 | 137.44 | 346.45 |
| CV (%) | 5.4% | 6.4% | 5% | 6.9% | 8% | 6.7% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Myeloperoxidase/MPO in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 354 | 346 | 367 | 309 | 344 | 21.55 | 6.2% |
| Sample 2 | 1809 | 1947 | 1689 | 1851 | 1824 | 92.61 | 5% |
| Sample 3 | 5328 | 4954 | 4733 | 4680 | 4923 | 255.01 | 5.1% |
Protein Target Info & Infographic
Gene/Protein Information For MPO (Source: Uniprot.Org, NCBI)
Gene Name
MPO
Full Name
Myeloperoxidase
Weight
83.869kDa
Superfamily
peroxidase family
Alternative Names
EC 1.11.1; EC 1.11.1.7; Lactoperoxidase; MPO; Myeloperoxidase MPO myeloperoxidase myeloperoxidase
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on MPO, check out the MPO Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for MPO: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse MPO/Myeloperoxidase ELISA Kit PicoKine® (EK0943)
Hello CJ!
EK0943 has been cited in 29 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
A Synthetic Receptor as a Specific Antidote for Paraquat Poisoning
β-Glucan Extracted from Highland Barley Alleviates Dextran Sulfate Sodium-Induced Ulcerative Colitis in C57BL/6J Mice
A Novel Peptide Ameliorates LPS-Induced Intestinal Inflammation and Mucosal Barrier Damage via Its Antioxidant and Antiendotoxin Effects
Combining the Specific Anti-MUC1 Antibody TAB004 and Lip-MSA-IL-2 Limits Pancreatic Cancer Progression in Immune Competent Murine Models of Pancreatic Ductal Adenocarcinoma
C-Terminal Amination of a Cationic Anti-Inflammatory Peptide Improves Bioavailability and Inhibitory Activity Against LPS-Induced Inflammation
A Novel Cecropin-LL37 Hybrid Peptide Protects Mice Against EHEC Infection-Mediated Changes in Gut Microbiota, Intestinal Inflammation, and Impairment of Mucosal Barrier Functions
Design and Development of a Novel Peptide for Treating Intestinal Inflammation
Protective effects of Huangqin Decoction against ulcerative colitis and associated cancer in mice
A novel pH‐controlled hydrogen sulfide donor protects gastric mucosa from aspirin‐induced injury
A highly efficient hybrid peptide ameliorates intestinal inflammation and mucosal barrier damage by neutralizing lipopolysaccharides and antagonizing the lipopolysaccharide‐receptor interaction
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Customer Reviews
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Customer Q&As
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11 Customer Q&As for Mouse MPO/Myeloperoxidase ELISA Kit PicoKine®
Question
Q: Can Myeloperoxidase ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
S. Zhou
Verified customer
Asked: 2020-04-25
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is a must. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2020-04-25
Question
Q: What is the optimal O.D. value for Myeloperoxidase ELISA kit? I performed your Myeloperoxidase ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain Myeloperoxidase even though the O.D. values are not very high?
B. Baker
Verified customer
Asked: 2019-11-27
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. considering your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Myeloperoxidase ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of Myeloperoxidase that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2019-11-27
Question
Our customer would like to know if this product can use tissue samples? https://www.bosterbio.com/mouse-mpo-picokine-trade-elisa-kit-ek0943-boster.html
Verified Customer
Verified customer
Asked: 2019-10-28
Answer
EK0943 hasn't been tested for any tissues. Endogenous biotin in tissue homogenates might introduce false positive signal. We recommend the customer run a pilot test as described below to confirm the affect of endogeous biotin first. Add tissue homogenates into the wells and then add ABC abd TMB without adding any biotinylated detection antibody to see if any signal will be observed. If no signal is produced, the customer can work on the tissue sample by using the kit.
Boster Scientific Support
Answered: 2019-10-28
Question
Is EK0943 suitable for lung fluid samples?
Verified customer
Asked: 2019-10-01
Answer
For the Mouse MPO/Myeloperoxidase ELISA Kit PicoKine™ (EK0943), we have not tested lung fluid samples with this kit, so we cannot guarantee that it would work. In theory the ELISA kit will work for all sample types if the target protein is present at a level that falls within the linear range of the ELISA kit detection range. We guarantee the kit to work on the sample types that we have tested. For sample types that we have not tested for, we suggest to run pilot experiments to decide the optimal sample dilution.
Boster Scientific Support
Answered: 2019-10-01
Question
Q: if the enzyme conjugated Myeloperoxidase antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Myeloperoxidase antigen?
Verified Customer
Verified customer
Asked: 2019-08-09
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2019-08-09
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2019-06-08
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2019-06-08
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
S. Collins
Verified customer
Asked: 2018-12-16
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2018-12-16
Question
Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?
T. Baker
Verified customer
Asked: 2018-11-29
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer may affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2018-11-29
Question
Q: can I use citrate plasma as samples in Mouse Myeloperoxidase Picokine® ELISA Kit (Catalog # EK0943)?
M. Garcia
Verified customer
Asked: 2018-02-23
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of Myeloperoxidase causing degradation of its protein structure. Myeloperoxidase may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the Myeloperoxidase ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2018-02-23
Question
Can the Mouse MPO PicoKine™ ELISA Kit be used on rat samples?
Verified Customer
Verified customer
Asked: 2017-08-21
Answer
It is not recommended to use Mouse MPO PicoKine™ ELISA Kit on rat samples.
Boster Scientific Support
Answered: 2017-08-21
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement Myeloperoxidase using the EK0943 Mouse Myeloperoxidase Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2017-03-26
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples to prevent protein degradation.
Boster Scientific Support
Answered: 2017-03-26
