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Product Info Summary
| SKU: | EK0943 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Mouse MPO/Myeloperoxidase ELISA Kit PicoKine®
SKU/Catalog Number
EK0943
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse MPO/Myeloperoxidase ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Mpo in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse MPO/Myeloperoxidase ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0943)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: M16-T718
Capture/Detection Antibodies
The capture antibody is polyclonal antibody from goat and the detection antibody is polyclonal antibody from goat.
Sensitivity
<10 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0943 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0943 is reactive to Mpo in Mouse samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK0943 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Mpo
Myeloperoxidase (MPO) is a mammalian phagocyte hemoprotein thought to primarily mediate host defense reactions. It is abundantly expressed in neutrophils and secreted during their activation. Myeloperoxidase is part of the host defense system of human polymorphonuclear leukocytes, responsible for microbicidal activity against a wide range of organisms. It is located in the nucleus as well as in the cytoplasm. Intranuclear MPO may help to protect DNA against damage resulting from oxygen radicals produced during myeloid cell maturation and function.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0943-CAP | Anti-Mouse Mpo Pre-coated 96-well strip microplate | 1 |
| EK0943-ST | Mouse Mpo Standard | 2 vials, 10 ng/tube |
| EK0943-DA | Mouse Mpo Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.038 | 0.155 | 0.261 | 0.449 | 0.831 | 1.277 | 1.825 | 2.085 |
Data Example Images
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Mouse MPO PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Mpo, Dilution ratio of 1:100, concentration in serum is around 100 ng/ml. .
Some articles we found to cite concentrations of Mpo in samples: 25337592 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse MPO/Myeloperoxidase ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 354 | 1809 | 5328 | 362 | 1718 | 5171 |
| Standard deviation | 19.11 | 115.77 | 266.4 | 346.457 | 137.44 | 346.45 |
| CV (%) | 5.4% | 6.4% | 5% | 6.9% | 8% | 6.7% |
Specific Publications For Mouse MPO/Myeloperoxidase ELISA Kit PicoKine® (EK0943)
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Customer Reviews
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Customer Q&As
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11 Customer Q&As for Mouse MPO/Myeloperoxidase ELISA Kit PicoKine®
Question
Q: Can Myeloperoxidase ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
S. Zhou
Verified customer
Asked: 2020-04-25
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is a must. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2020-04-25
Question
Q: What is the optimal O.D. value for Myeloperoxidase ELISA kit? I performed your Myeloperoxidase ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain Myeloperoxidase even though the O.D. values are not very high?
B. Baker
Verified customer
Asked: 2019-11-27
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. considering your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Myeloperoxidase ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of Myeloperoxidase that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2019-11-27
Question
Our customer would like to know if this product can use tissue samples? https://www.bosterbio.com/mouse-mpo-picokine-trade-elisa-kit-ek0943-boster.html
Verified Customer
Verified customer
Asked: 2019-10-28
Answer
EK0943 hasn't been tested for any tissues. Endogenous biotin in tissue homogenates might introduce false positive signal. We recommend the customer run a pilot test as described below to confirm the affect of endogeous biotin first. Add tissue homogenates into the wells and then add ABC abd TMB without adding any biotinylated detection antibody to see if any signal will be observed. If no signal is produced, the customer can work on the tissue sample by using the kit.
Boster Scientific Support
Answered: 2019-10-28
Question
Is EK0943 suitable for lung fluid samples?
Verified customer
Asked: 2019-10-01
Answer
For the Mouse MPO/Myeloperoxidase ELISA Kit PicoKine™ (EK0943), we have not tested lung fluid samples with this kit, so we cannot guarantee that it would work. In theory the ELISA kit will work for all sample types if the target protein is present at a level that falls within the linear range of the ELISA kit detection range. We guarantee the kit to work on the sample types that we have tested. For sample types that we have not tested for, we suggest to run pilot experiments to decide the optimal sample dilution.
Boster Scientific Support
Answered: 2019-10-01
Question
Q: if the enzyme conjugated Myeloperoxidase antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Myeloperoxidase antigen?
Verified Customer
Verified customer
Asked: 2019-08-09
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2019-08-09
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2019-06-08
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/elisa-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/elisa-data-analysis-instructions
Boster Scientific Support
Answered: 2019-06-08
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
S. Collins
Verified customer
Asked: 2018-12-16
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2018-12-16
Question
Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?
T. Baker
Verified customer
Asked: 2018-11-29
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer may affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2018-11-29
Question
Q: can I use citrate plasma as samples in Mouse Myeloperoxidase Picokine® ELISA Kit (Catalog # EK0943)?
M. Garcia
Verified customer
Asked: 2018-02-23
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of Myeloperoxidase causing degradation of its protein structure. Myeloperoxidase may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the Myeloperoxidase ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2018-02-23
Question
Can the Mouse MPO PicoKine™ ELISA Kit be used on rat samples?
Verified Customer
Verified customer
Asked: 2017-08-21
Answer
It is not recommended to use Mouse MPO PicoKine™ ELISA Kit on rat samples.
Boster Scientific Support
Answered: 2017-08-21
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement Myeloperoxidase using the EK0943 Mouse Myeloperoxidase Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2017-03-26
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples to prevent protein degradation.
Boster Scientific Support
Answered: 2017-03-26
