Mouse MPO/Myeloperoxidase ELISA Kit PicoKine™

Mouse MPO/Myeloperoxidase ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Mpo in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. Cited in 10 publication(s).

Product Info Summary

SKU: EK0943
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Mouse MPO/Myeloperoxidase ELISA Kit PicoKine™

See all Myeloperoxidase/MPO products

SKU/Catalog Number

EK0943

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse MPO/Myeloperoxidase ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Mpo in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Mouse MPO/Myeloperoxidase ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0943)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: M16-T718

Sensitivity

<10 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0943 is reactive to Mpo in Mouse samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK0943 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0943-CAP Anti-Mouse Mpo Pre-coated 96-well strip microplate 1
EK0943-ST Mouse Mpo Standard 2 vials, 10 ng/tube
EK0943-DA Mouse Mpo Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631262512502500500010000
O.D.0.0340.0700.1080.1840.4270.6601.3782.095

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse MPO/Myeloperoxidase ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)3541809532836217185171
Standard deviation19.11115.77266.4346.457137.44346.45
CV (%)5.4%6.4%5%6.9%8%6.7%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Myeloperoxidase/MPO in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 135434636730934421.556.2%
Sample 21809194716891851182492.615%
Sample 353284954473346804923255.015.1%
*number of samples for each test n=16.

Gene/Protein Information For Mpo (Source: Uniprot.Org, NCBI)

Uniprot ID

P11247

Gene ID

17523

Gene Name

Mpo

Full Name

Myeloperoxidase

Weight

81.182kDa

Superfamily

peroxidase family

Alternative Names

EC 1.11.1; EC 1.11.1.7; Lactoperoxidase; MPO; Myeloperoxidase Mpo|mKIAA4033|myeloperoxidase|myeloperoxidase

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Mpo, check out the Mpo Infographic

Mpo infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Mpo: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0943 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Porcine lactoferrin-derived peptide LFP-20 protects intestinal barrier by maintaining tight junction complex and modulating inflammatory response

Peptoanaerobacter stomatis Primes Human Neutrophils and Induces Granule Exocytosis

A novel pH?controlled hydrogen sulfide donor protects gastric mucosa from aspirin?induced injury

MicroRNA-206 is involved in the pathogenesis of ulcerative colitis via regulation of adenosine A3 receptor

Protective effects of Huangqin Decoction against ulcerative colitis and associated cancer in mice

Chen G, Yang Y, Liu M, Teng Z, Ye J, Xu Y, Cai X, Cheng X, Yang J, Hu C, Wang M, Cao P. J Ethnopharmacol. 2015 May 26;166:149-56. Doi: 10.1016/J.Jep.2015.03.027. Epub 2015 Mar 17. Banxia Xiexin Decoction Protects Against Dextran Sulfate Sodium-Ind...

Chen J, Chen X, Qin J. Brain Inj. 2011;25(3):292-9. Doi: 10.3109/02699052.2010.546824. Epub 2011 Jan 31. Effects Of Polysaccharides Of The Euphoria Longan (Lour.) Steud On Focal Cerebral Ischemia/Reperfusion Injury And Its Underlying Mechanism.

Wang W, Tang L, Li Y, Wang Y. J Neurol Sci. 2015 Jan 15;348(1-2):121-5. Doi: 10.1016/J.Jns.2014.11.018. Epub 2014 Nov 18. Biochanin A Protects Against Focal Cerebral Ischemia/Reperfusion In Rats Via Inhibition Of P38-Mediated Inflammatory Responses.

Li X, Liu Z, Jin H, Fan X, Yang X, Tang W, Yan J, Liang H. Biomed Res Int. 2014;2014:583736. Doi: 10.1155/2014/583736. Epub 2014 Aug 27. Agmatine Protects Against Zymosan-Induced Acute Lung Injury In Mice By Inhibiting Nf-??b-Mediated Inflammatory...

Wu Z, Ming Y, Zhang Z, Wu J, Wu Z, Yao S. Chin Med J (Engl). 2014;127(5):968-70. Edaravone Rescues The Lung By Inhibiting Lipid Peroxidation And Pro-Inflammatory Cytokines In A Rat Model.

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11 Customer Q&As for Mouse MPO/Myeloperoxidase ELISA Kit PicoKine™

Question

Q: Can Myeloperoxidase ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

S. Zhou

Verified customer

Asked: 2020-04-25

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is a must. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2020-04-25

Question

Q: What is the optimal O.D. value for Myeloperoxidase ELISA kit? I performed your Myeloperoxidase ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain Myeloperoxidase even though the O.D. values are not very high?

B. Baker

Verified customer

Asked: 2019-11-27

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. considering your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Myeloperoxidase ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of Myeloperoxidase that can be declared/interpreted as positive by the above standard is 10pg/ml.

Boster Scientific Support

Answered: 2019-11-27

Question

Our customer would like to know if this product can use tissue samples? https://www.bosterbio.com/mouse-mpo-picokine-trade-elisa-kit-ek0943-boster.html

Verified Customer

Verified customer

Asked: 2019-10-28

Answer

EK0943 hasn't been tested for any tissues. Endogenous biotin in tissue homogenates might introduce false positive signal. We recommend the customer run a pilot test as described below to confirm the affect of endogeous biotin first. Add tissue homogenates into the wells and then add ABC abd TMB without adding any biotinylated detection antibody to see if any signal will be observed. If no signal is produced, the customer can work on the tissue sample by using the kit.

Boster Scientific Support

Answered: 2019-10-28

Question

Is EK0943 suitable for lung fluid samples?

Verified customer

Asked: 2019-10-01

Answer

For the Mouse MPO/Myeloperoxidase ELISA Kit PicoKine™ (EK0943), we have not tested lung fluid samples with this kit, so we cannot guarantee that it would work. In theory the ELISA kit will work for all sample types if the target protein is present at a level that falls within the linear range of the ELISA kit detection range. We guarantee the kit to work on the sample types that we have tested. For sample types that we have not tested for, we suggest to run pilot experiments to decide the optimal sample dilution.

Boster Scientific Support

Answered: 2019-10-01

Question

Q: if the enzyme conjugated Myeloperoxidase antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Myeloperoxidase antigen?

Verified Customer

Verified customer

Asked: 2019-08-09

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2019-08-09

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

Verified Customer

Verified customer

Asked: 2019-06-08

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2019-06-08

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

S. Collins

Verified customer

Asked: 2018-12-16

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2018-12-16

Question

Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?

T. Baker

Verified customer

Asked: 2018-11-29

Answer

A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer may affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2018-11-29

Question

Q: can I use citrate plasma as samples in Mouse Myeloperoxidase Picokine® ELISA Kit (Catalog # EK0943)?

M. Garcia

Verified customer

Asked: 2018-02-23

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of Myeloperoxidase causing degradation of its protein structure. Myeloperoxidase may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the Myeloperoxidase ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2018-02-23

Question

Can the Mouse MPO PicoKine™ ELISA Kit be used on rat samples?

Verified Customer

Verified customer

Asked: 2017-08-21

Answer

It is not recommended to use Mouse MPO PicoKine™ ELISA Kit on rat samples.

Boster Scientific Support

Answered: 2017-08-21

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement Myeloperoxidase using the EK0943 Mouse Myeloperoxidase Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2017-03-26

Answer

A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples to prevent protein degradation.

Boster Scientific Support

Answered: 2017-03-26

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