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Product Info Summary
| SKU: | EK0481 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (EDTA). |
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Product info
Product Name
Mouse OPG (TNFRSF11B) / Osteoprotegerin ELISA Kit PicoKine®
View all Osteoprotegerin/TNFRSF11B ELISA kits
SKU/Catalog Number
EK0481
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse OPG (TNFRSF11B) / Osteoprotegerin ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Tnfrsf11b in cell culture supernatants, serum and plasma (EDTA). Sensitivity: 5pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse OPG (TNFRSF11B) / Osteoprotegerin ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0481)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: E22-L401
Sensitivity
<5 pg/ml
Assay Range
93.7 pg/ml - 6,000 pg/ml
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0481 is reactive to Tnfrsf11b in Mouse samples
Validated Sample Types
cell culture supernatants, serum and plasma (EDTA).
Application Guarantee
EK0481 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Osteoprotegerin/TNFRSF11B
Osteoprotegerin (OPG) is identical to osteoclastogenesis inhibitory factor (OCIF), a soluble member of the tumor-necrosis factor receptor family that inhibits osteoclastogenesis. OPG is considered to play an important role in the regulation of bone resorption by modifying osteoclast differentiation. Osteoprotegerin is an independent risk factor for the progression of atherosclerosis and onset of cardiovascular disease. It can act as a soluble factor in the regulation of bone mass and imply a utility for OPG in the treatment of osteoporosis associated with increased osteoclast activity. OPG system may play a critical role in the development of osteolytic bone disease in multiple myeloma and that targeting this system may have therapeutic potential. OPG also plays a role in the vascular system.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0481-CAP | Anti-Mouse Tnfrsf11b Pre-coated 96-well strip microplate | 1 |
| EK0481-ST | Mouse Tnfrsf11b Standard | 2 vials, 10 ng/tube |
| EK0481-DA | Mouse Tnfrsf11b Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 93.7 | 187.5 | 375 | 750 | 1500 | 3000 | 6000 |
| O.D. | 0.000 | 0.082 | 0.173 | 0.294 | 0.522 | 0.976 | 1.585 | 2.018 |
Data Example Images
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Mouse OPG (TNFRSF11B) PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Osteoprotegerin/TNFRSF11B, Dilution ratio of 1:1, concentration in serum is around 400pg/ml.
Some articles we found to cite concentrations of Osteoprotegerin/TNFRSF11B in samples: 23331792 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse OPG (TNFRSF11B) / Osteoprotegerin ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 133 | 800 | 3535 | 139 | 767 | 3643 |
| Standard deviation | 5.85 | 54.4 | 187.35 | 7.39 | 55.39 | 225.86 |
| CV (%) | 4.4% | 6.8% | 5.3% | 4.6% | 7.3% | 6.2% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Osteoprotegerin/TNFRSF11B in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 133 | 157 | 157 | 146 | 148 | 9.88 | 6.6% |
| Sample 2 | 800 | 766 | 743 | 710 | 754 | 32.84 | 4.3% |
| Sample 3 | 3535 | 3949 | 3581 | 3584 | 3662 | 166.69 | 4.5% |
Protein Target Info & Infographic
Gene/Protein Information For Tnfrsf11b (Source: Uniprot.Org, NCBI)
Uniprot ID
O08712
Gene ID
18383
Gene Name
Tnfrsf11b
Full Name
Tumor necrosis factor receptor superfamily member 11B
Weight
45.923kDa
Alternative Names
OCIF; OCIFMGC29565; OPGtumor necrosis factor receptor superfamily member 11B; Osteoclastogenesis inhibitory factor; Osteoprotegerin; TNFRSF11B; TR1; tumor necrosis factor receptor superfamily, member 11b Tnfrsf11b|O, OC, OCIF, Opg, TR, TR1|tumor necrosis factor receptor superfamily, member 11b (osteoprotegerin)|tumor necrosis factor receptor superfamily member 11B|osteoclastogenesis inhibitory factor|osteoprotegerin
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Tnfrsf11b, check out the Tnfrsf11b Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Tnfrsf11b: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
Specific Publications For Mouse OPG (TNFRSF11B) / Osteoprotegerin ELISA Kit PicoKine® (EK0481)
EK0481 has been cited in 13 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Liu Hui,Zhou Chuankun,Qi Dahu,Gao Yutong,Zhu Meipeng,Tao Tenghui,Sun Xuying,Xiao Jun,Inhibiting Monoacylglycerol Lipase Suppresses RANKL-Induced Osteoclastogenesis and Alleviates Ovariectomy-Induced Bone Loss.Frontiers in Cell and Developmental Biology,VOLUME=9,YEAR=2021, PAGES=544, URL=https://www.frontiersin.org/article/10.3389/fcell.2021.640867. DOI=10.3389/fcell.2021.640867,ISSN=2296-634X
Species: Mouse
EK0481 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA
Inhibition of PRMT5 suppresses osteoclast differentiation and partially protects against ovariectomy-induced bone loss through downregulation of CXCL10 and RSAD2
Bone loss caused by dopaminergic degeneration and levodopa treatment in Parkinson's disease model mice
Loss of RUNX3 expression inhibits bone invasion of oral squamous cell carcinoma
Amlexanox Suppresses Osteoclastogenesis and Prevents Ovariectomy-Induced Bone Loss
SIRT6 Deficiency Culminates in Low-Turnover Osteopenia
Zhao L, Cai C, Wang J, Zhao L, Li W, Liu C, Guan H, Zhu Y, Xiao J. Front Pharmacol. 2017 Dec 19;8:928. doi: 10.3389/fphar.2017.00928. eCollection 2017. Dihydromyricetin Protects against Bone Loss in Ovariectomized Mice by Suppressing Osteoclast Ac...
Guo J, Li W, Wu Y, Jing X, Huang J, Zhang J, Xiang W, Ren R, Lv Z, Xiao J, Guo F. Front Pharmacol. 2017 Oct 4;8:693. doi: 10.3389/fphar.2017.00693. eCollection 2017. Meclizine Prevents Ovariectomy-Induced Bone Loss and Inhibits Osteoclastogenesis ...
Wu, H., Wu, Z., Li, P., Cong, Q., Chen, R., Xu, W.,…, & Li, B. (2017). Bone Size and Quality Regulation: Concerted Actions of mTOR in Mesenchymal Stromal Cells and Osteoclasts. Stem Cell Reports, 8(6), 1600-1616. doi: 10.1016/j.stemcr.2017.04.005.
Montesi M, Panseri S, Iafisco M, Adamiano A, Tampieri A. Plos One. 2015 Jul 6;10(7):E0132633. Doi: 10.1371/Journal.Pone.0132633. Ecollection 2015. Coupling Hydroxyapatite Nanocrystals With Lactoferrin As A Promising Strategy To Fine Regulate Bone ...
Mai Qg, Zhang Zm, Xu S, Lu M, Zhou Rp, Zhao L, Jia Ch, Wen Zh, Jin Dd, Bai Xc. J Cell Biochem. 2011 Oct;112(10):2902-9. Doi: 10.1002/Jcb.23206. Metformin Stimulates Osteoprotegerin And Reduces Rankl Expression In Osteoblasts And Ovariectomized Rats.
Xu S, Wang Y, Lu J, Xu J. Rheumatol Int. 2012 Nov;32(11):3397-403. Doi: 10.1007/S00296-011-2175-5. Epub 2011 Nov 6. Osteoprotegerin And Rankl In The Pathogenesis Of Rheumatoid Arthritis-Induced Osteoporosis.
Chen B, Li Xd, Liu Dx, Wang H, Xie P, Liu Zy, Hou Gq, Chang B, Du Sx. Phytomedicine. 2012 Aug 15;19(11):1029-34. Doi: 10.1016/J.Phymed.2012.06.002. Epub 2012 Jul 19. Canonical Wnt Signaling Is Required For Panax Notoginseng Saponin-Mediated Attenu...
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
13 Customer Q&As for Mouse OPG (TNFRSF11B) / Osteoprotegerin ELISA Kit PicoKine®
Question
Q: Can Osteoprotegerin ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2020-11-03
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is needed. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please check the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2020-11-03
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
V. Hernandez
Verified customer
Asked: 2020-07-10
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2020-07-10
Question
We currently used your Mouse OPG (TNFRSF11B) / Osteoprotegerin ELISA Kit for testing in mouse plasma. I was wondering if there is any cross-reactivity with these antibody to human plasma.
Verified Customer
Verified customer
Asked: 2020-04-23
Answer
The antibody Mouse OPG (TNFRSF11B) / Osteoprotegerin ELISA Kit, the capture antibody is 100% cross reactivity with human OPG however, the detection antibody shows close to 0%, so when it used as a pair we cannot expect significant cross reactivity with human OPG.
Boster Scientific Support
Answered: 2020-04-23
Question
Q: can I use heparin plasma as samples in Mouse Osteoprotegerin Picokine® ELISA Kit (Catalog # EK0481)?
Verified Customer
Verified customer
Asked: 2020-04-02
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of Osteoprotegerin causing degradation of its protein structure. Osteoprotegerin may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the Osteoprotegerin ELISA, treating samples with different anticoagulants and decided that EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2020-04-02
Question
We used your Mouse OPG (TNFRSF11B) / Osteoprotegerin PicoKine
Verified Customer
Verified customer
Asked: 2019-12-31
Answer
Unfortunately, our records shows 0% cross reactivity of this FEK0481 antibody with human
Boster Scientific Support
Answered: 2019-12-31
Question
Q: which procedure should I follow in order to thaw whole blood sample for Osteoprotegerin ELISA after freezing?
D. Johnson
Verified customer
Asked: 2019-12-17
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Osteoprotegerin for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2019-12-17
Question
Q: if the enzyme conjugated Osteoprotegerin antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Osteoprotegerin antigen?
Verified Customer
Verified customer
Asked: 2019-03-03
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2019-03-03
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement Osteoprotegerin using the EK0481 Mouse Osteoprotegerin Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2018-11-15
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to avoid protein degradation.
Boster Scientific Support
Answered: 2018-11-15
Question
Q: What is the optimal O.D. value for Osteoprotegerin ELISA kit? I performed your Osteoprotegerin ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain Osteoprotegerin even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2018-06-09
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Osteoprotegerin ELISA kit to have sensitivity of 5pg/ml, that means the minimum amount of Osteoprotegerin that can be declared/interpreted as positive by the above standard is 5pg/ml.
Boster Scientific Support
Answered: 2018-06-09
Question
Q: can you suggest the dilution ratio of serum samples for detection of Osteoprotegerin in Mouse serum? I am trying to measure a multiple analytes and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.
Z. Varadkar
Verified customer
Asked: 2017-06-07
Answer
A: unable to understand the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Osteoprotegerin ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2017-06-07
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
C. Singh
Verified customer
Asked: 2016-02-13
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2016-02-13
Question
Dear Support: I would like to ask whether Mouse OPG (TNFRSF11B) / Osteoprotegerin PicoKine
Verified Customer
Verified customer
Asked: 2015-11-25
Answer
The Mouse OPG (TNFRSF11B) / Osteoprotegerin PicoKine
Boster Scientific Support
Answered: 2015-11-25
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Z. Wilson
Verified customer
Asked: 2014-09-26
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2014-09-26
