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Mouse OPG (TNFRSF11B) / Osteoprotegerin ELISA Kit PicoKine™
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse OPG (TNFRSF11B). 96wells/kit, with removable strips. Cited in 11 publication(s).
Mouse OPG (TNFRSF11B) / Osteoprotegerin ELISA Kit PicoKine™ Info At A Glance
| SKU: | EK0481 |
|---|---|
| Size: | 96wells/kit, with removable strips. |
| Reactivity: | Mouse |
| Sample Type: | cell culture supernates, serum and plasma(EDTA). |
| Sample Volume: | 100μl per well |
| Sensitivity: | <5pg/ml |
| Assay Range: | 93.7pg/ml-6000pg/ml |
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Overview
| Product Name | Mouse OPG (TNFRSF11B) / Osteoprotegerin ELISA Kit PicoKine™
See all Tnfrsf11b primary antibodies, ELISA kits and proteins |
|---|---|
| SKU/Catalog Number | EK0481 |
| Storage & Handling | Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.) |
| Description | Detect Mouse Osteoprotegerin/TNFRSF11B with <5pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, serum and plasma(EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Osteoprotegerin/TNFRSF11B tissue specificity: Highly expressed in liver, lung, stomach, intestines and calvaria. Highly expressed in decidua and placenta, and in embryo. |
| Cite This Product | Mouse OPG (TNFRSF11B) / Osteoprotegerin ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0481) |
Product Specs
| Reactivity/Species | Mouse |
|---|---|
| Applications | ELISA
*Our Boster Guarantee covers the use of this product in the above tested applications. **For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab. |
| Sample Type | cell culture supernates, serum and plasma(EDTA). |
| Sensitivity | <5pg/ml |
| Assay Range | 93.7pg/ml-6000pg/ml |
| Immunogen | Expression system for standard: NSO; Immunogen sequence: E22-L401 |
| Antibody Clonalities | Capture antibody|Detection antibody monoclonal antibody from rat|polyclonal antibody from goat |
| Cross Reactivity | There is no detectable cross-reactivity with other relevant proteins. |
| Pack Size | 96wells/kit, with removable strips. |
Protein Target Info (Source: Uniprot.org)
| Uniprot Id | O08712 |
|---|---|
| Gene Name | Tnfrsf11b |
| Protein Name | Tumor necrosis factor receptor superfamily member 11B |
| Subcellular Localization | Secreted. |
*if product is indicated to react with multiple species, protein info is based on the human gene.
Ontology
| Protein Function | Acts as decoy receptor for TNFSF11/RANKL and thereby neutralizes its function in osteoclastogenesis. Inhibits the activation of osteoclasts and promotes osteoclast apoptosis in vitro. Bone homeostasis seems to depend on the local ratio between TNFSF11 and TNFRSF11B. May also play a role in preventing arterial calcification. May act as decoy receptor for TNFSF10/TRAIL and protect against apoptosis. TNFSF10/TRAIL binding blocks the inhibition of osteoclastogenesis. . |
|---|---|
| Background | Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. Functions as a hormone. |
Kit Components And Assay QC Data Details Of Mouse OPG (TNFRSF11B) / Osteoprotegerin ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
| Catalog number | Description | Quantity |
|---|---|---|
| EK0481-CAP | 96-well plate precoated with anti-Mouse Tnfrsf11b antibody | 1 |
| EK0481-ST | lyophilized recombinant Mouse Tnfrsf11b standard | 10ng/tube |
| EK0481-DA | biotinylated anti-Mouse Tnfrsf11b antibody | 130ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 130ul |
| AR1106-1 | sample diluent buffer | 30ml |
| AR1106-2 | antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EK0481 Mouse OPG (TNFRSF11B) / Osteoprotegerin ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
| Concentration(pg/ml) | 0 | 93.7 | 187.5 | 375 | 750 | 13000 | 3000 | 6000 |
| O.D. | 0.000 | 0.082 | 0.173 | 0.294 | 0.522 | 0.976 | 1.585 | 2.118 |
We measured random samples of Mouse OPG (TNFRSF11B) / Osteoprotegerin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 133 | 800 | 3535 | 139 | 767 | 3643 |
| Standard deviation | 5.85 | 54.4 | 187.35 | 7.39 | 55.39 | 225.86 |
| CV(%) | 4.4% | 6.8% | 5.3% | 4.6% | 7.3% | 6.2% |
We measured the performance consistency of Mouse OPG (TNFRSF11B) / Osteoprotegerin ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 133 | 157 | 157 | 146 | 148 | 9.88 | 6.6% |
| Sample 2 | 800 | 766 | 743 | 710 | 754 | 32.84 | 4.3% |
| Sample 3 | 3535 | 3949 | 3581 | 3584 | 3662 | 166.69 | 4.5% |
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
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Total number of citations: 11
| Chen B, Li Xd, Liu Dx, Wang H, Xie P, Liu Zy, Hou Gq, Chang B, Du Sx. Phytomedicine. 2012 Aug 15;19(11):1029-34. Doi: 10.1016/J.Phymed.2012.06.002. Epub 2012 Jul 19. Canonical Wnt Signaling Is Required For Panax Notoginseng Saponin-Mediated Attenu... | PubMed ID 22818895 |
| Xu S, Wang Y, Lu J, Xu J. Rheumatol Int. 2012 Nov;32(11):3397-403. Doi: 10.1007/S00296-011-2175-5. Epub 2011 Nov 6. Osteoprotegerin And Rankl In The Pathogenesis Of Rheumatoid Arthritis-Induced Osteoporosis. | PubMed ID 22057136 |
| Mai Qg, Zhang Zm, Xu S, Lu M, Zhou Rp, Zhao L, Jia Ch, Wen Zh, Jin Dd, Bai Xc. J Cell Biochem. 2011 Oct;112(10):2902-9. Doi: 10.1002/Jcb.23206. Metformin Stimulates Osteoprotegerin And Reduces Rankl Expression In Osteoblasts And Ovariectomized Rats. | PubMed ID 21618594 |
| Montesi M, Panseri S, Iafisco M, Adamiano A, Tampieri A. Plos One. 2015 Jul 6;10(7):E0132633. Doi: 10.1371/Journal.Pone.0132633. Ecollection 2015. Coupling Hydroxyapatite Nanocrystals With Lactoferrin As A Promising Strategy To Fine Regulate Bone ... | PubMed ID 26148296 |
| Wu, H., Wu, Z., Li, P., Cong, Q., Chen, R., Xu, W.,…, & Li, B. (2017). Bone Size and Quality Regulation: Concerted Actions of mTOR in Mesenchymal Stromal Cells and Osteoclasts. Stem Cell Reports, 8(6), 1600-1616. doi: 10.1016/j.stemcr.2017.04.005. | PubMed ID 28479301 |
| Guo J, Li W, Wu Y, Jing X, Huang J, Zhang J, Xiang W, Ren R, Lv Z, Xiao J, Guo F. Front Pharmacol. 2017 Oct 4;8:693. doi: 10.3389/fphar.2017.00693. eCollection 2017. Meclizine Prevents Ovariectomy-Induced Bone Loss and Inhibits Osteoclastogenesis ... | PubMed ID 29046637 |
| Zhao L, Cai C, Wang J, Zhao L, Li W, Liu C, Guan H, Zhu Y, Xiao J. Front Pharmacol. 2017 Dec 19;8:928. doi: 10.3389/fphar.2017.00928. eCollection 2017. Dihydromyricetin Protects against Bone Loss in Ovariectomized Mice by Suppressing Osteoclast Ac... | PubMed ID 29311931 |
| SIRT6 Deficiency Culminates in Low-Turnover Osteopenia | PubMed ID 26189760 |
| Amlexanox Suppresses Osteoclastogenesis and Prevents Ovariectomy-Induced Bone Loss | PubMed ID 26338477 |
| Loss of RUNX3 expression inhibits bone invasion of oral squamous cell carcinoma | PubMed ID 28030842 |
| Bone loss caused by dopaminergic degeneration and levodopa treatment in Parkinson's disease model mice | PubMed ID 31551490 |
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Domestically in USA and Canada, ships next business day if available in stock.
Note: sample size takes longer to process, average 5-7 days.
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| (888) 466-3604 |
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