Mouse PECAM-1 / CD31 / PECAM1 ELISA Kit PicoKine™
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse PECAM-1/CD31. 96wells/kit, with removable strips. Cited in 2 publication(s).
Mouse PECAM-1 / CD31 / PECAM1 ELISA Kit PicoKine™ Info At A Glance
|Size:||96wells/kit, with removable strips.|
|Sample Type:||cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).|
|Sample Volume:||100μl per well|
|Product Name||Mouse PECAM-1 / CD31 / PECAM1 ELISA Kit PicoKine™
See all CD31/PECAM-1 primary antibodies, ELISA kits and proteins
|Storage & Handling||Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)|
|Description||Detect Mouse Cd31/PECAM1 with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Cd31/PECAM1 tissue specificity: Isoform 1 and isoform 3 are expressed in lung and platelets. .|
|Cite This Product||Mouse PECAM-1 / CD31 / PECAM1 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0874)|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab.
|Sample Type||cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).|
|Immunogen/Standard||Expression system for standard: NSO; Immunogen sequence: E18-K590|
|Antibody Clonalities||Capture antibody|Detection antibody
monoclonal antibody from rat|polyclonal antibody from goat
|Cross Reactivity||There is no detectable cross-reactivity with other relevant proteins.|
|Pack Size||96wells/kit, with removable strips.|
Gene/Protein Basic Information For Pecam1 (Source: Uniprot.org, NCBI)
|NCBI Gene Id||18613|
|Species Of This Entry||Mouse|
|Protein Name||Platelet endothelial cell adhesion molecule|
|Alternative Names||CD31/PECAM-1|adhesion molecule; CD31 antigen; CD31; CD31/EndoCAM; EndoCAM; FLJ34100; FLJ58394; GPIIA'; PECA1; PECAM1; PECAM-1; PECAM-1, CD31/EndoCAM; platelet endothelial cell adhesion molecule; platelet/endothelial cell adhesion molecule|
|Post Tranlational Modifications||Phosphorylation; Cleavage|
|Gene Location||11|11 E1, on Chromasome 11, gene sequence:|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For Pecam1 (Source: Uniprot.org, NCBI)
|Protein Function||Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions (By similarity). Tyr-679 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes (By similarity). Trans-homophilic interaction may play a role in endothelial cell-cell adhesion via cell junctions (By similarity). Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils (By similarity). Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal (By similarity). Promotes macrophage-mediated phagocytosis of apoptotic leukocytes by tethering them to the phagocytic cells; PECAM1-mediated detachment signal appears to be disabled in apoptotic leukocytes (By similarity). Modulates bradykinin receptor BDKRB2 activation (By similarity). Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in endothelial cells (By similarity). Induces susceptibility to atherosclerosis (PubMed:19048083).|
|Tissue Specificity||Isoform 1 and isoform 3 are expressed in lung and platelets.|
|Related Diseases||Tissue Adhesions; Tumor Angiogenesis; Pathologic Neovascularization; Neoplasms; Inflammation; Malignant Neoplasms; Von Willebrand Disease; Infarction; Myocardial Infarction; Atherosclerosis; Malaria; Thrombosis; Sclerosis; Coronary Heart Disease; Carcinoma; Fibrosis; Diabetes Mellitus; Heart Diseases; Multiple Sclerosis; Malaria, Falciparum|
|Related Pathways||Cell Adhesion; Angiogenesis; Localization; Vasculogenesis; Pathogenesis; Cell Migration; Cell Division; Cell Differentiation; Cell-cell Adhesion; Cell Death; Dedifferentiation; Secretion; Transport; Cell Activation; T Cell Activation; Tube Formation; Fibroblast Proliferation; Immune Response; Hypersensitivity; Lymphangiogenesis|
|Background||PECAM-1 is an important target GP in DITP.1 Platelet/endothelial cell adhesion molecule-1(PECAM-1) is a 130-kD member of the Ig gene superfamily that is expressed on the surface of circulating platelets, monocytes, neutrophils, and selective T-cell subsets.2 Platelet endothelial cell adhesion molecule-1(PECAM-1/CD31) is a member of the immunoglobulin(Ig) superfamily that has distinctive features of an immunoreceptor based upon its genomic structure and the presence of intrinsic immunoreceptor tyrosine inhibitory motifs(ITIMs) in its ligand binding polypeptide.3 PECAM-1 has recently been shown to contain functional immunoreceptor tyrosine-based inhibitory motifs(ITIMs) within its cytoplasmic domain, and co-ligation of PECAM-1 with the T-cell antigen receptor(TCR) results in tyrosine phosphorylation of PECAM-1, recruitment of Src homology 2 domain-containing protein tyrosine phosphatase-2(SHP-2), and attenuation of TCR-mediated cellular signaling.4 The standard used in this kit is the product of gene recombinant expression(extracellular part), consisting of 563 amino acids(E28-K590) with the molecular weight of 65.5KDa. After glycosylating, its molecular weight changes to 91-100KDa.|
|Scientific References||PMID: 8516303 by Xie Y., et al. Molecular cloning and adhesive properties of murine platelet/endothelial cell adhesion molecule 1.|
PMID: 1415479 by Bogen S.A., et al. Association of murine CD31 with transmigrating lymphocytes following antigenic stimulation.
Kit Components And Assay QC Data Details Of Mouse PECAM-1 / CD31 / PECAM1 ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
|EK0874-CAP||96-well plate precoated with anti-Mouse CD31/PECAM-1 antibody||1|
|EK0874-ST||lyophilized recombinant Mouse CD31/PECAM-1 standard||10ng/tube|
|EK0874-DA||biotinylated anti-Mouse CD31/PECAM-1 antibody||130ul|
|AR1106-1||sample diluent buffer||30ml|
|AR1106-2||antibody diluent buffer||12ml|
|AR1106-3||ABC diluent buffer||12ml|
|AR1104||TMB color developing agent||10ml|
|AR1105||TMB stop solution||10ml|
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EK0874 Mouse PECAM-1 / CD31 / PECAM1 ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
We measured random samples of Mouse PECAM-1 / CD31 / PECAM1 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We measured the performance consistency of Mouse PECAM-1 / CD31 / PECAM1 ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
|Lots||Lot1 (pg/ml)||Lot2 (pg/ml)||Lot3 (pg/ml)||Lot4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
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Total number of citations: 2
|Wang Tb, Wei Xq, Lin Wh, Shi Hp, Dong Wg. Clin Exp Med. 2012 Jun;12(2):89-95. Doi: 10.1007/S10238-011-0143-8. Epub 2011 Jun 10. The Inhibition Of Endostar On The Angiogenesis And Growth Of Gastrointestinal Stromal Tumor Xenograft.||PubMed ID 21660447|
|Yang S, Zhu X, Cai L, Cheng H, Zhao R, Wang H, Zhao H, Wang Z. Cancer Sci. 2010 Mar;101(3):679-85. Doi: 10.1111/J.1349-7006.2009.01436.X. Epub 2009 Nov 14. Role Of Tumor-Associated Lymphatic Endothelial Cells In Metastasis: A Study Of Epithelial O...||PubMed ID 20028387|
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