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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).|
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Mouse PECAM-1 / CD31 / PECAM1 ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Mouse PECAM-1 / CD31 / PECAM1 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Pecam1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse PECAM-1 / CD31 / PECAM1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0874)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: E18-K590
156 pg/ml - 10,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK0874 is reactive to Pecam1 in Mouse samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
EK0874 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of CD31/PECAM-1
PECAM-1 is an important target GP in DITP.1 Platelet/endothelial cell adhesion molecule-1 (PECAM-1) is a 130-kD member of the Ig gene superfamily that is expressed on the surface of circulating platelets, monocytes, neutrophils, and selective T-cell subsets.2 Platelet endothelial cell adhesion molecule-1 (PECAM-1/CD31) is a member of the immunoglobulin (Ig) superfamily that has distinctive features of an immunoreceptor based upon its genomic structure and the presence of intrinsic immunoreceptor tyrosine inhibitory motifs (ITIMs) in its ligand binding polypeptide.3 PECAM-1 has recently been shown to contain functional immunoreceptor tyrosine-based inhibitory motifs (ITIMs) within its cytoplasmic domain, and co-ligation of PECAM-1 with the T-cell antigen receptor (TCR) results in tyrosine phosphorylation of PECAM-1, recruitment of Src homology 2 domain-containing protein tyrosine phosphatase-2 (SHP-2), and attenuation of TCR-mediated cellular signaling.4 The standard used in this kit is the product of gene recombinant expression (extracellular part), consisting of 563 amino acids (E28-K590) with the molecular weight of 65.5KDa. After glycosylating, its molecular weight changes to 91-100KDa.
|EK0874-CAP||Anti-Mouse Pecam1 Pre-coated 96-well strip microplate||1|
|EK0874-ST||Mouse Pecam1 Standard||2 vials, 10 ng/tube|
|EK0874-DA||Mouse Pecam1 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Mouse PECAM-1/CD31 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect CD31/PECAM-1, Dilution ratio of 1:20, concentration in serum is 27.5-48.2ng/ml.
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse PECAM-1 / CD31 / PECAM1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of CD31/PECAM-1 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For Pecam1 (Source: Uniprot.Org, NCBI)
Platelet endothelial cell adhesion molecule
adhesion molecule; CD31 antigen; CD31; CD31/EndoCAM; EndoCAM; FLJ34100; FLJ58394; GPIIA'; PECA1; PECAM1; PECAM-1; PECAM-1, CD31/EndoCAM; platelet endothelial cell adhesion molecule; platelet/endothelial cell adhesion molecule Pecam1|C85791, Cd31, PECAM-1, Pecam|platelet/endothelial cell adhesion molecule 1|platelet endothelial cell adhesion molecule*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Pecam1, check out the Pecam1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Pecam1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
EK0874 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Yang S, Zhu X, Cai L, Cheng H, Zhao R, Wang H, Zhao H, Wang Z. Cancer Sci. 2010 Mar;101(3):679-85. Doi: 10.1111/J.1349-7006.2009.01436.X. Epub 2009 Nov 14. Role Of Tumor-Associated Lymphatic Endothelial Cells In Metastasis: A Study Of Epithelial O...
Wang Tb, Wei Xq, Lin Wh, Shi Hp, Dong Wg. Clin Exp Med. 2012 Jun;12(2):89-95. Doi: 10.1007/S10238-011-0143-8. Epub 2011 Jun 10. The Inhibition Of Endostar On The Angiogenesis And Growth Of Gastrointestinal Stromal Tumor Xenograft.
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