Mouse PECAM-1 / CD31 / PECAM1 ELISA Kit PicoKine™

Mouse PECAM-1 / CD31 / PECAM1 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Pecam1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. Cited in 2 publication(s).

Product Info Summary

SKU: EK0874
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Mouse PECAM-1 / CD31 / PECAM1 ELISA Kit PicoKine™

See all CD31/PECAM-1 products

SKU/Catalog Number

EK0874

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse PECAM-1 / CD31 / PECAM1 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Pecam1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Mouse PECAM-1 / CD31 / PECAM1 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0874)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: E18-K590

Sensitivity

<10 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0874 is reactive to Pecam1 in Mouse samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK0874 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0874-CAP Anti-Mouse Pecam1 Pre-coated 96-well strip microplate 1
EK0874-ST Mouse Pecam1 Standard 2 vials, 10 ng/tube
EK0874-DA Mouse Pecam1 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631262512500025000500010000
O.D.0.0320.1490.2450.4130.7511.2461.9522.170

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect CD31/PECAM-1, Dilution ratio of 1:20, concentration in serum is 27.5-48.2ng/ml.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse PECAM-1 / CD31 / PECAM1 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)2281326566323013985316
Standard deviation17.3274.25407.7318.1799.25409.33
CV (%)7.6%5.6%7.2%7.9%7.1%7.7%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of CD31/PECAM-1 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 122821722119921610.74.9%
Sample 21326135911601320129177.215.9%
Sample 356636219581453405759315.995.4%
*number of samples for each test n=16.

Gene/Protein Information For Pecam1 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q08481

Gene ID

18613

Gene Name

Pecam1

Full Name

Platelet endothelial cell adhesion molecule

Weight

81.263kDa

Alternative Names

adhesion molecule; CD31 antigen; CD31; CD31/EndoCAM; EndoCAM; FLJ34100; FLJ58394; GPIIA'; PECA1; PECAM1; PECAM-1; PECAM-1, CD31/EndoCAM; platelet endothelial cell adhesion molecule; platelet/endothelial cell adhesion molecule Pecam1|C85791, Cd31, PECAM-1, Pecam|platelet/endothelial cell adhesion molecule 1|platelet endothelial cell adhesion molecule

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Pecam1, check out the Pecam1 Infographic

Pecam1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Pecam1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0874 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Yang S, Zhu X, Cai L, Cheng H, Zhao R, Wang H, Zhao H, Wang Z. Cancer Sci. 2010 Mar;101(3):679-85. Doi: 10.1111/J.1349-7006.2009.01436.X. Epub 2009 Nov 14. Role Of Tumor-Associated Lymphatic Endothelial Cells In Metastasis: A Study Of Epithelial O...

Wang Tb, Wei Xq, Lin Wh, Shi Hp, Dong Wg. Clin Exp Med. 2012 Jun;12(2):89-95. Doi: 10.1007/S10238-011-0143-8. Epub 2011 Jun 10. The Inhibition Of Endostar On The Angiogenesis And Growth Of Gastrointestinal Stromal Tumor Xenograft.

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6 Customer Q&As for Mouse PECAM-1 / CD31 / PECAM1 ELISA Kit PicoKine™

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

L. Hernandez

Verified customer

Asked: 2020-09-08

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is a must for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2020-09-08

Question

Q: What is the optimal O.D. value for CD31 ELISA kit? I used your CD31 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain CD31 even though the O.D. values are not very high?

Verified Customer

Verified customer

Asked: 2020-08-01

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. considering your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this CD31 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of CD31 that can be declared/interpreted as positive by the above standard is 10pg/ml.

Boster Scientific Support

Answered: 2020-08-01

Question

Q: how to thaw whole blood sample for CD31 ELISA after freezing?

H. Green

Verified customer

Asked: 2019-05-06

Answer

A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test CD31 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2019-05-06

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

M. Moore

Verified customer

Asked: 2019-03-11

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2019-03-11

Question

Q: if the enzyme conjugated CD31 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the CD31 antigen?

Verified Customer

Verified customer

Asked: 2018-08-19

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2018-08-19

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

M. Wang

Verified customer

Asked: 2014-03-24

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2014-03-24

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