Mouse RANK / CD265 ELISA Kit PicoKine™

Mouse RANK / CD265 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Tnfrsf11a in cell culture supernatants and serum. Sensitivity: 2pg/ml. Cited in 1 publication(s).

Product Info Summary

SKU: EK0830
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants and serum.

Product Name

Mouse RANK / CD265 ELISA Kit PicoKine™

See all RANK/TNFRSF11A products

SKU/Catalog Number

EK0830

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse RANK / CD265 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Tnfrsf11a in cell culture supernatants and serum. Sensitivity: 2pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Mouse RANK / CD265 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0830)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q30-P213

Sensitivity

<2 pg/ml

Assay Range

62.5 pg/ml - 4,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0830 is reactive to Tnfrsf11a in Mouse samples

Validated Sample Types

cell culture supernatants and serum.

Application Guarantee

EK0830 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0830-CAP Anti-Mouse Tnfrsf11a Pre-coated 96-well strip microplate 1
EK0830-ST Mouse Tnfrsf11a Standard 2 vials, 10 ng/tube
EK0830-DA Mouse Tnfrsf11a Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)04000200040002000400020004000
O.D.0.0060.1090.2050.4320.7191.1841.6592.038

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect RANK/TNFRSF11A, Dilution ratio of 1:1, concentration in serum is 200-1000pg/ml.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse RANK / CD265 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)2107191032218725978
Standard deviation11.9730.1946.4415.8%542.0553.79
CV (%)5.7%4.2%4.5%7.5%5.8%5.5%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of RANK/TNFRSF11A in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 121021619818620211.525.7%
Sample 271968570766669420.362.9%
Sample 31032104610901088106425.492.3%
*number of samples for each test n=16.

Gene/Protein Information For Tnfrsf11a (Source: Uniprot.Org, NCBI)

Uniprot ID

O35305

Gene ID

21934

Gene Name

Tnfrsf11a

Full Name

Tumor necrosis factor receptor superfamily member 11A

Weight

66.621kDa

Alternative Names

CD265 antigen; CD265; FEO; loss of heterozygosity, 18, chromosomal region 1; ODFR; ODFROSTS; OFE; OPTB7; Osteoclast differentiation factor receptor; PDB2; RANK; RANKLOH18CR1; Receptor activator of NF-KB; receptor activator of nuclear factor-kappa B; TNFRSF11A; TRANCE R; TRANCER; tumor necrosis factor receptor superfamily member 11A; tumor necrosis factor receptor superfamily, member 11a, activator of NFKB; tumor necrosis factor receptor superfamily, member 11a, NFKB activator Tnfrsf11a|Ly109, ODFR, OFE, R, Rank, TRAN, TRANCE-R, mRANK|tumor necrosis factor receptor superfamily, member 11a, NFKB activator|tumor necrosis factor receptor superfamily member 11A|osteoclast differentiation factor receptor|receptor activator of NF-KB|receptor activator of NF-kappaB

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Tnfrsf11a, check out the Tnfrsf11a Infographic

Tnfrsf11a infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Tnfrsf11a: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0830 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Xiaogang Wang,Yuanman Yu,Luli Ji,Zhen Geng,Jing Wang,Changsheng Liu,Calcium phosphate-based materials regulate osteoclast-mediated osseointegration,Bioactive Materials,Volume 6,Issue 12,2021,Pages 4517-4530,ISSN 2452-199X,https://doi.org/10.1016/j.bioactmat.2021.05.003.
Species: Mouse
EK0830 usage in article: APP:ELISA, SAMPLE:BMMS, DILUTION:NA

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5 Customer Q&As for Mouse RANK / CD265 ELISA Kit PicoKine™

Question

Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring RANK using the EK0830 Mouse RANK Picokine® ELISA Kit?

G. Green

Verified customer

Asked: 2018-10-25

Answer

A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples to prevent protein degradation.

Boster Scientific Support

Answered: 2018-10-25

Question

Q: we need your recommendation regarding the dilution ratio of serum samples for detection of RANK in Mouse serum? I am trying to measure a a number of analytes and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.

D. Varma

Verified customer

Asked: 2018-04-03

Answer

A: unable to understand the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the RANK ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2018-04-03

Question

Q: Can RANK ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

Verified Customer

Verified customer

Asked: 2016-01-25

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is a must. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2016-01-25

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

Verified Customer

Verified customer

Asked: 2016-01-11

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2016-01-11

Question

Q: if the enzyme conjugated RANK antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the RANK antigen?

Verified Customer

Verified customer

Asked: 2015-07-11

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2015-07-11

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