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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants and serum.|
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Mouse RANK / CD265 ELISA Kit PicoKine™
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Mouse RANK / CD265 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Tnfrsf11a in cell culture supernatants and serum. Sensitivity: 2pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Cite This Product
Mouse RANK / CD265 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0830)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: Q30-P213
62.5 pg/ml - 4,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK0830 is reactive to Tnfrsf11a in Mouse samples
Validated Sample Types
cell culture supernatants and serum.
EK0830 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
|EK0830-CAP||Anti-Mouse Tnfrsf11a Pre-coated 96-well strip microplate||1|
|EK0830-ST||Mouse Tnfrsf11a Standard||2 vials, 10 ng/tube|
|EK0830-DA||Mouse Tnfrsf11a Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Mouse RANK PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect RANK/TNFRSF11A, Dilution ratio of 1:1, concentration in serum is 200-1000pg/ml.
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse RANK / CD265 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of RANK/TNFRSF11A in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For Tnfrsf11a (Source: Uniprot.Org, NCBI)
Tumor necrosis factor receptor superfamily member 11A
CD265 antigen; CD265; FEO; loss of heterozygosity, 18, chromosomal region 1; ODFR; ODFROSTS; OFE; OPTB7; Osteoclast differentiation factor receptor; PDB2; RANK; RANKLOH18CR1; Receptor activator of NF-KB; receptor activator of nuclear factor-kappa B; TNFRSF11A; TRANCE R; TRANCER; tumor necrosis factor receptor superfamily member 11A; tumor necrosis factor receptor superfamily, member 11a, activator of NFKB; tumor necrosis factor receptor superfamily, member 11a, NFKB activator Tnfrsf11a|Ly109, ODFR, OFE, R, Rank, TRAN, TRANCE-R, mRANK|tumor necrosis factor receptor superfamily, member 11a, NFKB activator|tumor necrosis factor receptor superfamily member 11A|osteoclast differentiation factor receptor|receptor activator of NF-KB|receptor activator of NF-kappaB*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Tnfrsf11a, check out the Tnfrsf11a Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Tnfrsf11a: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
EK0830 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Xiaogang Wang,Yuanman Yu,Luli Ji,Zhen Geng,Jing Wang,Changsheng Liu,Calcium phosphate-based materials regulate osteoclast-mediated osseointegration,Bioactive Materials,Volume 6,Issue 12,2021,Pages 4517-4530,ISSN 2452-199X,https://doi.org/10.1016/j.bioactmat.2021.05.003.
EK0830 usage in article: APP:ELISA, SAMPLE:BMMS, DILUTION:NA
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