Mouse TGF Beta 1 ELISA Kit PicoKine™

Mouse TGF Beta 1 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Tgfb1 in cell culture supernatants, serum, plasma (EDTA) and urine. Sensitivity: 2pg/ml. Cited in 44 publication(s).

Product Info Summary

SKU: EK0515
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, serum, plasma (EDTA) and urine.

Product Name

Mouse TGF Beta 1 ELISA Kit PicoKine™

See all Tgfb1 products

SKU/Catalog Number

EK0515

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse TGF Beta 1 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Tgfb1 in cell culture supernatants, serum, plasma (EDTA) and urine. Sensitivity: 2pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Mouse TGF Beta 1 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0515)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: CHO; Immunogen sequence: A279-S390

Sensitivity

<2 pg/ml

Assay Range

15.6 pg/ml - 1,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0515 is reactive to Tgfb1 in Mouse samples

Validated Sample Types

cell culture supernatants, serum, plasma (EDTA) and urine.

Application Guarantee

EK0515 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0515-CAP Anti-Mouse Tgfb1 Pre-coated 96-well strip microplate 1
EK0515-ST Mouse Tgfb1 Standard 2 vials, 10 ng/tube
EK0515-DA Mouse Tgfb1 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015.631.262.51252505001000
O.D.0.0570.1380.2200.4060.6111.0601.6122.047

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse TGF Beta 1 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)3616949933183484
Standard deviation1.4711.6631.431.5512.9934.36
CV (%)4.1%6.9%7.1%4.7%7.1%7.1%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Tgfb1 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 136394238382.165.6%
Sample 21691691841721736.183.5%
Sample 349944252149048828.855.9%
*number of samples for each test n=16.

Gene/Protein Information For Tgfb1 (Source: Uniprot.Org, NCBI)

Uniprot ID

P04202

Gene ID

21803

Gene Name

Tgfb1

Full Name

Transforming growth factor beta-1 proprotein

Weight

44.31kDa

Superfamily

TGF-beta family

Alternative Names

CED; DPD1; LAP; TGFB; TGFB1; TGF-beta 1, 2, 3; TGFbeta; transforming growth factor, beta 1 Tgfb1|TGF-beta, TGF-beta1, TGFbeta, TGFbeta1, Tgfb, Tgfb-1|transforming growth factor, beta 1|transforming growth factor beta-1 proprotein

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Tgfb1, check out the Tgfb1 Infographic

Tgfb1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Tgfb1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0515 has been cited in 44 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Low-Dose Anti-Angiogenic Therapy Sensitizes Breast Cancer to PD-1 Blockade
Species: Mouse

Unsaturated polyurethane films grafted with enantiomeric polylysine promotes macrophage polarization to a M2 phenotype through PI3K/Akt1/mTOR axis
Species: Mouse

In vivo restoration of full-thickness cartilage defects by poly(lactide-co-glycolide) sponges filled with fibrin gel, bone marrow mesenchymal stem cells and DNA complexes
Species: Mouse

Clara cell 10-kDa protein inhibits TH17 responses through modulating dendritic cells in the setting of allergic rhinitis
Species: Mouse

Chen J,Wang H,Jia L,He J,Li Y,Liu H,Wu R,Qiu Y,Zhan Y,Yuan Z,Cao Y,Li W,Xu K,Yin P.Bufalin targets the SRC-3/MIF pathway in chemoresistant cells to regulate M2 macrophage polarization in colorectal cancer.Cancer Lett.2021 May 14:S0304-3835(21)00223-8.doi:10.1016/j.canlet.2021.05.008.Epub ahead of print.PMID:34000344.
Species: Human,Mouse
EK0515 usage in article: APP:ELISA, SAMPLE: SERUM, DILUTION:NA

Yilun Luo,Xiaowen Zheng,Peiqi Yuan,Xingyao Ye,Lie Ma,Light-induced dynamic RGD pattern for sequential modulation of macrophage phenotypes,Bioactive Materials,Volume 6,Issue 11,2021,Pages 4065-4072,ISSN 2452-199X,https://doi.org/10.1016/j.bioactmat.2021.04.018.
Species: Mouse
EK0515 usage in article: APP:ELISA, SAMPLE:BMDM, DILUTION:NA

Duan Y,Zheng H,Li Z,Yao Y,Ding J,Wang X,Nakkala JR,Zhang D,Wang Z,Zuo X,Zheng X,Ling J,Gao C.Unsaturated polyurethane films grafted with enantiomeric polylysine promotes macrophage polarization to a M2 phenotype through PI3K/Akt1/mTOR axis.Biomaterials.2020 Jul;246:120012.doi:10.1016/j.biomaterials.2020.120012.Epub 2020 Mar 31.PMID:32276198.
Species: Mouse
EK0515 usage in article: APP:ELISA, SAMPLE:MACROPHAGES, DILUTION:NA

Li-Bo Jiang, Sheng-Long Ding, Wang Ding, Di-Han Su, Fang-Xue Zhang, Tai-Wei Zhang, Xiao-Fan Yin, Lan Xiao, Yu-Lin Li, Feng-Lai Yuan, Jian Dong, Injectable Sericin Based Nanocomposite Hydrogel for Multi-modal Imaging-guided Immunomodulatory Bone Regeneration,Chemical Engineering Journal,2021,129323,ISSN 1385-8947,https://doi.org/10.1016/j.cej.2021.129323.
Species: Mouse,Rat
EK0515 usage in article: APP:ELISA, SAMPLE:RAW264.7 CELL, DILUTION:NA

Attia YM, Tawfiq RA, Gibriel AA, Ali AA, Kassem DH, Hammam OA, Elmazar MM. Activation of FXR modulates SOCS3/Jak2/STAT3 signaling axis in a NASH-dependent hepatocellular carcinoma animal model. Biochem Pharmacol. 2021 Mar 3:114497. doi: 10.1016/j.bcp.2021.114497. Epub ahead of print. PMID: 33675775.
Species: Mouse
EK0515 usage in article: APP:ELISA, SAMPLE:LIVER TISSUE, DILUTION:NA

Morrone,Carmela (2020):Cathepsin B, a new player in the progression of Bronchiolitis obliterans syndrome.Dissertation,LMU München:Faculty of Medicine
Species: Mouse
EK0515 usage in article: APP:ELISA, SAMPLE:RAW264.7 CELLS, DILUTION:NA

Liu YN,Guan Y,Shen J,Jia YL,Zhou JC,Sun Y,Jiang JX,Shen HJ,Shu Q,Xie QM,Xie Y. Shp2 positively regulates cigarette smoke-induced epithelial mesenchymal transition by mediating MMP-9 production. Respir Res. 2020 Jun 26;21(1):161.doi:10.1186/s12931-020-0142
Species: Human,Mouse
EK0515 usage in article: APP:ELISA, SAMPLE:NCI-H292 CELL, DILUTION:NA

Zhu J, Yang LK, Chen WL, Lin W, Wang YH, Chen T. Activation of SK/KCa Channel Attenuates Spinal Cord Ischemia-Reperfusion Injury via Anti-oxidative Activity and Inhibition of Mitochondrial Dysfunction in Rabbits. Front Pharmacol. 2019 Apr 2;10:325. doi: 1
Species: Rabbit
EK0515 usage in article: APP:ELISA, SAMPLE:SPINAL CORD, DILUTION:NA

Inhibitive effect of cordyceps sinensis on experimental hepatic fibrosis and its possible mechanism

Protective immunity against acute toxoplasmosis in BALB/c mice induced by a DNA vaccine encoding?Toxoplasma gondii?elongation factor 1-alpha

Signaling via the CXCR5/ERK pathway is mediated by CXCL13 in mice with breast cancer

LincRNA 1700020I14Rik alleviates cell proliferation and fibrosis in diabetic nephropathy via miR-34a-5p/Sirt1/HIF-1? signaling

Toxoplasma gondii excretory/secretory antigens (TgESAs) suppress pro-inflammatory cytokine secretion by inhibiting TLR-induced NF-?B activation in LPS-stimulated murine macrophages

Ilomastat, a synthetic inhibitor of MMPs, prevents lung injury induced by ?-ray irradiation in mice

Effects of Recombinant Toxoplasma gondii Citrate Synthase I on the Cellular Functions of Murine Macrophages In vitro

Oroxylin A inhibits the generation of Tregs in non-small cell lung cancer

The effects of 1,4-dimethylpyridine in metastatic prostate cancer in mice

Toxoplasma gondii Elongation Factor 1-Alpha (TgEF-1?) Is a Novel Vaccine Candidate Antigen against Toxoplasmosis

Mature dendritic?cells?cause Th17/Treg imbalance by secreting TGF-?1 and IL-6 in the pathogenesis of experimental autoimmune encephalomyelitis

A deleterious role for Th9/IL-9 in hepatic fibrogenesis

Herbal Compound Songyou Yin and Moderate Swimming Suppress Growth and Metastasis of Liver Cancer by Enhancing Immune Function

Fluorofenidone Offers Improved Renoprotection at Early Interventions during the Course of Diabetic Nephropathy in?db/db?Mice via Multiple Pathways

DNA vaccination with a gene encoding?Toxoplasma gondii?Deoxyribose Phosphate Aldolase (TgDPA) induces partial protective immunity against lethal challenge in mice

Exogenous interleukin-10 alleviates allergic inflammation but inhibits local interleukin-10 expression in a mouse allergic rhinitis model

Ganoderma lucidum

Santagata S, Napolitano M, D'Alterio C, Desicato S, Maro SD, Marinelli L, Fragale A, Buoncervello M, Persico F, Gabriele L, Novellino E, Longo N, Pignata S, Perdon

Denslow A, ?witalska M, Jarosz J, Papiernik D, Porshneva K, Nowak M, Wietrzyk J. PLoS One. 2017 Dec 5;12(12):e0188740. doi: 10.1371/journal.pone.0188740. eCollection 2017. Clopidogrel in a combined therapy with anticancer drugs

Zhou XM, Wang GL, Wang XB, Liu L, Zhang Q, Yin Y, Wang QY, Kang J, Hou G. Front Pharmacol. 2017 Dec 12;8:904. doi: 10.3389/fphar.2017.00904. eCollection 2017. GHK Peptide Inhibits Bleomycin-Induced Pulmonary Fibrosis in Mice by Suppressing TGFβ1/S...

Chen Y, Zeng C, Zhan Y, Wang H, Jiang X, Li W. Oncogene. 2017 Aug 17;36(33):4692-4705. doi: 10.1038/onc.2017.100. Epub 2017 Apr 10. Aberrant low expression of p85α in stromal fibroblasts promotes breast cancer cell metastasis through exosome-media...

Yang N, Zhang H, Cai X, Shang Y. Int J Mol Med. 2018 Feb;41(2):818-828. doi: 10.3892/ijmm.2017.3292. Epub 2017 Nov 28. Epigallocatechin-3-gallate inhibits inflammation and epithelial-mesenchymal transition through the PI3K/AKT pathway via upregula...

Wang W, Liu M, Wang Y, Yang T, Li D, Ding F, Sun H, Bai G, Li Q. Evid Based Complement Alternat Med. 2018 Jan 28;2018:2305683. doi: 10.1155/2018/2305683. eCollection 2018. Lycium barbarum Polysaccharide Promotes Maturation of Dendritic Cell via No...

Li, J, Liu, X., Duan, Y., Liu, Y., Wang, H., Lian, S.,..., & Fan, Y. (2017). Combined Blockade of T Cell Immunoglobulin and Mucin Domain 3 and Carcinoembryonic Antigen-Related Cell Adhesion Molecule 1 Results in Durable Therapeutic Efficacy in Mic...

Kabel, A.M., & Elkhoely, A.A. (2017). Targeting proinflammatory cytokines, oxidative stress, TGF-β1 and STAT-3 by rosuvastatin and ubiquinone to ameliorate trastuzumab cardiotoxicity. Biomedicine & Pharmacotherapy, 93, 17-26. Advance online public...

Yang L, Ni L, Duan Q, Wang X, Chen C, Chen S, Chaugai S, Zeldin Dc, Tang Jr, Wang Dw. Prostaglandins Other Lipid Mediat. 2015 Jan-Mar;116-117:64-75. Doi: 10.1016/J.Prostaglandins.2015.01.004. Epub 2015 Feb 14. Cyp Epoxygenase 2J2 Prevents Cardiac ...

Li B, Yang J, Ma L, Li F, Tu Z, Gao C. J Biomed Mater Res A. 2013 Nov;101(11):3097-108. Doi: 10.1002/Jbm.A.34618. Epub 2013 Mar 25. Fabrication Of Poly(Lactide-Co-Glycolide) Scaffold Filled With Fibrin Gel, Mesenchymal Stem Cells, And Poly(Ethylen...

Wang Z, Jinnin M, Kobayashi Y, Kudo H, Inoue K, Nakayama W, Honda N, Makino K, Kajihara I, Makino T, Fukushima S, Inagaki Y, Ihn H. J Dermatol Sci. 2015 Sep;79(3):268-78. Doi: 10.1016/J.Jdermsci.2015.06.008. Epub 2015 Jun 22. Mice Overexpressing I...

Sharifi N, Amani R, Hajiani E, Cheraghian B. Endocrine. 2014 Sep;47(1):70-80. Doi: 10.1007/S12020-014-0336-5. Epub 2014 Jun 27. Does Vitamin D Improve Liver Enzymes, Oxidative Stress, And Inflammatory Biomarkers In Adults With Non-Alcoholic Fatty ...

Huang Xq, Zhang Xy, Wang Xr, Yu Sy, Fang Sh, Lu Yb, Zhang Wp, Wei Eq. J Neuroinflammation. 2012 Jun 26;9:145. Doi: 10.1186/1742-2094-9-145. Transforming Growth Factor ??1-Induced Astrocyte Migration Is Mediated In Part By Activating 5-Lipoxygenase...

Gao L, Huang W, Li J. Mol Cell Biochem. 2013 Oct;382(1-2):185-91. Doi: 10.1007/S11010-013-1733-4. Epub 2013 Jun 26. Nox1 Abet Mesangial Fibrogenesis Via Inos Induction In Diabetes.

Wu Yh, Hu Sq, Liu J, Cao Hc, Xu W, Li Yj, Li Lj. Int J Mol Med. 2014 Jun;33(6):1498-506. Doi: 10.3892/Ijmm.2014.1730. Epub 2014 Apr 7. Nature And Mechanisms Of Hepatocyte Apoptosis Induced By D-Galactosamine/Lipopolysaccharide Challenge In Mice.

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16 Customer Q&As for Mouse TGF Beta 1 ELISA Kit PicoKine™

Question

Q: if the enzyme conjugated TGFB1 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the TGFB1 antigen?

T. Phillips

Verified customer

Asked: 2020-11-10

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2020-11-10

Question

Q: Can TGFB1 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

W. Zhao

Verified customer

Asked: 2020-10-07

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is needed. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2020-10-07

Question

Q: What is the optimal O.D. value for TGFB1 ELISA kit? I used your TGFB1 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain TGFB1 even though the O.D. values are not very high?

Verified Customer

Verified customer

Asked: 2020-07-05

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. in your example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this TGFB1 ELISA kit to have sensitivity of 2pg/ml, that means the minimum amount of TGFB1 that can be declared/interpreted as positive by the above standard is 2pg/ml.

Boster Scientific Support

Answered: 2020-07-05

Question

Is your mouse monoclonal to TGF-beta in the kit specific to mouse, or will it cross with human TGF-beta or Bovine TGF-beta that may be present in the media from the FBS? Keyword: specificity

Verified Customer

Verified customer

Asked: 2019-11-26

Answer

TGFb1 is conservative, EK0515 also cross reacts with human and bovine TGFb1.

Boster Scientific Support

Answered: 2019-11-26

Question

What does the EK0515 kit detect after sample´s activation?

Verified customer

Asked: 2019-11-11

Answer

The EK0515 Mouse TGF Beta 1 ELISA Kit PicoKine detects Active TGF beta after actitvating the sample.

Boster Scientific Support

Answered: 2019-11-12

Question

Does your mouse TGF-beta kit detect TGF-beta when in complex with its binding proteins or latent complex? Do I have to perform some kind of extraction or treat their supernatants with RIPA or other preparation to expose the actual free TGF-beta or will your monoclonal detect the TGF beta even when it is in the latent forms? Keyword: applications, protocol, detection

Verified Customer

Verified customer

Asked: 2019-10-14

Answer

TGFβ1 is mostly contained as inactive form in samples, please activate it before assay. Don't activate recombinant TGFβ1. Please contact us for recommended protocol to activate the sample (if want to analyze the active form).

Boster Scientific Support

Answered: 2019-10-14

Question

What is a reference range for mouse serum samples? Keyword: experimental data

Verified Customer

Verified customer

Asked: 2019-09-26

Answer

TGF-β1 level in mouse serum is about 80-123ng/ml.

Boster Scientific Support

Answered: 2019-09-26

Question

What is the protocol for performing EK0515 to test for the active form of TGF-b (acidification of the tissue homogenate sample)?

Verified customer

Asked: 2019-09-03

Answer

For the Mouse TGF Beta 1 ELISA Kit PicoKine™ (EK0515), please see the following protocol for activating tissue homogenate samples; 1. Mince tissue and then rinse tissue in ice-cold PBS. 2. Place minced tissue in tissue homogenizer. 3. Add ice-cold PBS. For a 0.5g piece of tissue, add 5ml of ice-cold PBS(Volumes of PBS must be determined in relation to the amount of tissue present.) 5. Homogenize for several minutes at high speed. 6. Sonicate until no tissue chunks remain. 7. Centrifuge at ~10000 x g for 10 minutes. The centrifugation force and time can be varied depending on the sample type. 8. Aspirate supernatant. 10.Add activating reagent into the supernatant pro rata, i.e. add 20μl of Solution A into 100μl of sample, 10 min later, add 20μl of Solution B. PH 7.0-7.6. Solution A: 1N HCl: add 8.33ml of 12N HCl into 91.67ml of H2O. Solution B: 1.2N NaOH/0.5M HEPES: add 12ml of 10N NaOH and 11.9g HEPES into 75ml of H2O, add H2O to adjust volume to 100ml. Note: Sample was diluted partly after adding activating reagent, so please pay attention to this when calculate target protein concentration. Please perform the endogenous biotin test first.

Boster Scientific Support

Answered: 2019-09-03

Question

Will EK0515 work for mouse tracheal tissue homogenates with samples approximately 4mm^3?

Verified customer

Asked: 2019-07-30

Answer

For the Mouse TGF Beta 1 ELISA Kit PicoKine™ (EK0515), the mouse tracheal tissue homogenates may work regardless of sample size as long as the endogenous biotin test has been done first.

Boster Scientific Support

Answered: 2019-07-31

Question

Q: we need your recommendation regarding the dilution ratio of serum samples for detection of TGFB1 in Mouse urine? I am trying to measure a multiple analytes and it requires 100ul of diluted samples for each well. We have low serum quantitys so we like to dilute as much as possible.

H. Perez

Verified customer

Asked: 2019-04-30

Answer

A: unable to understand the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the TGFB1 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2019-04-30

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2018-12-24

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is a must for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2018-12-24

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

H. Martinez

Verified customer

Asked: 2018-09-02

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2018-09-02

Question

Q: can I use heparin plasma as samples in Mouse TGFB1 Picokine® ELISA Kit (Catalog # EK0515)?

W. Davis

Verified customer

Asked: 2018-08-14

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of TGFB1 causing degradation of its protein structure. TGFB1 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the TGFB1 ELISA, treating samples with a number of anticoagulants and decided that EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2018-08-14

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification TGFB1 using the EK0515 Mouse TGFB1 Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2017-07-25

Answer

A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples to limit protein degradation.

Boster Scientific Support

Answered: 2017-07-25

Question

Thanks for the info. Would you be able to provide a protocol for performing the ELISA to test for the active form of TGF-b (acidification of the tissue homogenate sample)?

Verified Customer

Verified customer

Asked: 2015-03-11

Answer

Please see the following protocol for activating tissue homogenate samples 1. Mince tissue and then rinse tissue in ice-cold PBS. 2. Place minced tissue in tissue homogenizer. 3. Add ice-cold PBS. For a 0.5g piece of tissue, add 5ml of ice-cold PBS(Volumes of PBS must be determined in relation to the amount of tissue present.) 5. Homogenize for several minutes at high speed. 6. Sonicate until no tissue chunks remain. 7. Centrifuge at ~10000 x g for 10 minutes. The centrifugation force and time can be varied depending on the sample type. 8. Aspirate supernatant. 10.Add activating reagent into the supernatant pro rata, i.e. add 20?l of Solution A into 100?l of sample, 10 min later, add 20?l of Solution B. PH 7.0-7.6. Solution A: 1N HCl: add 8.33ml of 12N HCl into 91.67ml of H2O. Solution B: 1.2N NaOH/0.5M HEPES: add 12ml of 10N NaOH and 11.9g HEPES into 75ml of H2O, add H2O to adjust volume to 100ml. Note: Sample was diluted partly after adding activating reagent, so please pay attention to this when calculate target protein concentration. Please perform the endogenous biotin test first.

Boster Scientific Support

Answered: 2015-03-11

Question

I'm interested in purchasing the Mouse TGF Beta 1 PicoKine

Verified Customer

Verified customer

Asked: 2013-04-17

Answer

We haven't tested mouse tracheal tissue homogenates with EK0412. Endogenous biotin in tissue homogenates might introduce false positive signal. We recommend the customer run a pilot test as described below to confirm the affect of endogeous biotin first. Add tissue homogenates into the wells and then add ABC abd TMB without adding any biotinylated detection antibody to see if any signal will be observed. If no signal is produced, the customer can work on the tissue sample by using the kit.

Boster Scientific Support

Answered: 2013-04-17

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