Mouse TNFSF11/RANKL ELISA Kit PicoKine™

Mouse TNFSF11/RANKL ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Tnfsf11 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. Cited in 12 publication(s).

Product Info Summary

SKU: EK0843
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Mouse TNFSF11/RANKL ELISA Kit PicoKine™

See all TRANCE/TNFSF11/RANK L products

SKU/Catalog Number

EK0843

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse TNFSF11/RANKL ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Tnfsf11 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Mouse TNFSF11/RANKL ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0843)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: R72-D316

Sensitivity

<10 pg/ml

Assay Range

15.6 pg/ml - 1,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0843 is reactive to Tnfsf11 in Mouse samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK0843 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0843-CAP Anti-Mouse Tnfsf11 Pre-coated 96-well strip microplate 1
EK0843-ST Mouse Tnfsf11 Standard 2 vials, 10 ng/tube
EK0843-DA Mouse Tnfsf11 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015.631.262.51252505001000
O.D.0.0400.1180.1540.2850.4300.7921.3222.032

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect TRANCE/TNFSF11/RANK L, Dilution ratio of 1:1, concentration in serum is 30-290pg/ml.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse TNFSF11/RANKL ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)3413243032199419
Standard deviation1.4210.7217.631.7212.1321.36
CV (%)4.2%5.5%4.1%5.4%6.1%5.1%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of TRANCE/TNFSF11/RANK L in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 134393237352.697.6%
Sample 21951912011861935.492.8%
Sample 343047548048246621.374.5%
*number of samples for each test n=16.

Gene/Protein Information For Tnfsf11 (Source: Uniprot.Org, NCBI)

Uniprot ID

O35235

Gene ID

21943

Gene Name

Tnfsf11

Full Name

Tumor necrosis factor ligand superfamily member 11

Weight

35.003kDa

Superfamily

tumor necrosis factor family

Alternative Names

CD254 antigen; CD254; ODF; OPGL; OPGLOPTB2; Osteoclast differentiation factor; Osteoprotegerin ligand; RANK L; RANKL; RANKLreceptor activator of nuclear factor kappa B ligand; Receptor activator of nuclear factor kappa-B ligand; sOdf; TNF-related activation-induced cytokine; TNFSF11; TRANCE; TRANCEODFhRANKL2; tumor necrosis factor (ligand) superfamily, member 11; tumor necrosis factor ligand superfamily member 11 Tnfsf11|Ly10, Ly109l, O, OD, ODF, OPGL, R, RANKL, Tra, Trance|tumor necrosis factor (ligand) superfamily, member 11|tumor necrosis factor ligand superfamily member 11|OPG ligand|TNF-related activation-induced cytokine|osteoclast differentiation factor|osteoprotegerin ligand|receptor activator of NF-kappaB ligand|receptor activator of nuclear factor kappa-B ligand|tumor necrosis factor-related activation-induced cytokine

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Tnfsf11, check out the Tnfsf11 Infographic

Tnfsf11 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Tnfsf11: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0843 has been cited in 12 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Liu Hui,Zhou Chuankun,Qi Dahu,Gao Yutong,Zhu Meipeng,Tao Tenghui,Sun Xuying,Xiao Jun,Inhibiting Monoacylglycerol Lipase Suppresses RANKL-Induced Osteoclastogenesis and Alleviates Ovariectomy-Induced Bone Loss.Frontiers in Cell and Developmental Biology,VOLUME=9,YEAR=2021, PAGES=544, URL=https://www.frontiersin.org/article/10.3389/fcell.2021.640867. DOI=10.3389/fcell.2021.640867,ISSN=2296-634X
Species: Mouse
EK0843 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

REV-ERB agonism suppresses osteoclastogenesis and prevents ovariectomy-induced bone loss partially via FABP4 upregulation

An in vitro 3D bone metastasis model by using a human bone tissue culture and human sex-related cancer cells

Amlexanox Suppresses Osteoclastogenesis and Prevents Ovariectomy-Induced Bone Loss

Zhao L, Cai C, Wang J, Zhao L, Li W, Liu C, Guan H, Zhu Y, Xiao J. Front Pharmacol. 2017 Dec 19;8:928. doi: 10.3389/fphar.2017.00928. eCollection 2017. Dihydromyricetin Protects against Bone Loss in Ovariectomized Mice by Suppressing Osteoclast Ac...

Guo J, Li W, Wu Y, Jing X, Huang J, Zhang J, Xiang W, Ren R, Lv Z, Xiao J, Guo F. Front Pharmacol. 2017 Oct 4;8:693. doi: 10.3389/fphar.2017.00693. eCollection 2017. Meclizine Prevents Ovariectomy-Induced Bone Loss and Inhibits Osteoclastogenesis ...

Tang Y, Sun F, Li X, Zhou Y, Yin S, Zhou X. J Endod. 2011 Dec;37(12):1653-8. Doi: 10.1016/J.Joen.2011.08.015. Epub 2011 Oct 2. Porphyromonas Endodontalis Lipopolysaccharides Induce Rankl By Mouse Osteoblast In A Way Different From That Of Escheric...

Zhao H, Ning Ll, Wang Zy, Li Ht, Qiao D, Yao Y, Qin Hl. Cell Biochem Biophys. 2014 Nov;70(2):1097-104. Doi: 10.1007/S12013-014-0028-Z. Calcitonin Gene-Related Peptide Inhibits Osteolytic Factors Induced By Osteoblast In Co-Culture System With Brea...

Tang Zn, Zhang F, Tang P, Qi Xw, Jiang J. Biochem Biophys Res Commun. 2011 May 13;408(3):411-6. Doi: 10.1016/J.Bbrc.2011.04.035. Epub 2011 Apr 13. Hypoxia Induces Rank And Rankl Expression By Activating Hif-1?? In Breast Cancer Cells.

Montesi M, Panseri S, Iafisco M, Adamiano A, Tampieri A. Plos One. 2015 Jul 6;10(7):E0132633. Doi: 10.1371/Journal.Pone.0132633. Ecollection 2015. Coupling Hydroxyapatite Nanocrystals With Lactoferrin As A Promising Strategy To Fine Regulate Bone ...

Xu S, Wang Y, Lu J, Xu J. Rheumatol Int. 2012 Nov;32(11):3397-403. Doi: 10.1007/S00296-011-2175-5. Epub 2011 Nov 6. Osteoprotegerin And Rankl In The Pathogenesis Of Rheumatoid Arthritis-Induced Osteoporosis.

Chen B, Li Xd, Liu Dx, Wang H, Xie P, Liu Zy, Hou Gq, Chang B, Du Sx. Phytomedicine. 2012 Aug 15;19(11):1029-34. Doi: 10.1016/J.Phymed.2012.06.002. Epub 2012 Jul 19. Canonical Wnt Signaling Is Required For Panax Notoginseng Saponin-Mediated Attenu...

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7 Customer Q&As for Mouse TNFSF11/RANKL ELISA Kit PicoKine™

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2020-07-11

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2020-07-11

Question

Q: Can TRANCE ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

Verified Customer

Verified customer

Asked: 2020-02-18

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2020-02-18

Question

Will EK0843 react with human samples?

Verified Customer

Verified customer

Asked: 2019-08-09

Answer

EK0843 does not react with human samples.

Boster Scientific Support

Answered: 2019-08-09

Question

Q: how to analyze ELISA data? I have performed TRANCE level in cell lysates.

C. Bhatt

Verified customer

Asked: 2019-01-26

Answer

A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2019-01-26

Question

To Technical Support: I am interested to use this Mouse TNFSF11/RANKL ELISA Kit but I would like to know the minimum amount to run the sample. I will use na

Verified Customer

Verified customer

Asked: 2017-09-19

Answer

It is not possible that we can predict the minimum amount to be use in the samples as the amount of RANKL varies between experimental populations. Stated in the datasheets the sensitivity of this Mouse TNFSF11/RANKL ELISA Kit is <10pg/ml, so if you know how much RANK is present in your sample then it would be possible to calculate the minimum amount. However, we can recommend to run the technique of quick ELISA with your samples. Quick ELISA is when you run your top, middle, bottom and blank standards in the first four wells of the first column on the plate. Then you can use the next four columns in the plate to set up a serial dilution of your samples. Just run the ELISA in those wells only and cut all the incubation times in half. Through this it would give you an idea of the dilution you need to use in order to get your samples to fall within the standard curve.

Boster Scientific Support

Answered: 2017-09-19

Question

As we know that RANKL presents on cell membrane cleaved by ADAM17 and in cytosol as soluble form. Could you tell me whether Mouse TNFSF11/RANKL ELISA Kit reacts with soluble form and membrane-binding form?

Verified Customer

Verified customer

Asked: 2017-04-20

Answer

The Mouse TNFSF11/RANKL ELISA Kit detects the membrane bound form of RANKL, however it does not detect the soluble form. Do check these publication for your further references; Rubin et al. 2004. Prostate Carcinoma Cells that Have Resided in Bone have an Upregulated IGF-IAxis. The Prostate 58:41-49. In this study they used the same clone antibody to detect the membrane bound form of RANKL (mRANKL) and an antibody from another source to detect the soluble form of RANKL (sRANKL)

Boster Scientific Support

Answered: 2017-04-20

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

Verified Customer

Verified customer

Asked: 2016-11-26

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2016-11-26

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