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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).|
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Mouse TNFSF11/RANKL ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Mouse TNFSF11/RANKL ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Tnfsf11 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse TNFSF11/RANKL ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0843)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: R72-D316
15.6 pg/ml - 1,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK0843 is reactive to Tnfsf11 in Mouse samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
EK0843 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of TRANCE/TNFSF11/RANK L
Receptor activator of nuclear factor kappa-B ligand (RANKL), also known as tumor necrosis factor ligand superfamily member 11 (TNFSF11), is a protein that in humans is encoded by the TNFSF11 gene. This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This gene is mapped to chromosome13q14.11. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. This gene may play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy.
|EK0843-CAP||Anti-Mouse Tnfsf11 Pre-coated 96-well strip microplate||1|
|EK0843-ST||Mouse Tnfsf11 Standard||2 vials, 10 ng/tube|
|EK0843-DA||Mouse Tnfsf11 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Mouse TNFSF11/RANKL PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect TRANCE/TNFSF11/RANK L, Dilution ratio of 1:1, concentration in serum is 30-290pg/ml.
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse TNFSF11/RANKL ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of TRANCE/TNFSF11/RANK L in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For Tnfsf11 (Source: Uniprot.Org, NCBI)
Tumor necrosis factor ligand superfamily member 11
tumor necrosis factor family
CD254 antigen; CD254; ODF; OPGL; OPGLOPTB2; Osteoclast differentiation factor; Osteoprotegerin ligand; RANK L; RANKL; RANKLreceptor activator of nuclear factor kappa B ligand; Receptor activator of nuclear factor kappa-B ligand; sOdf; TNF-related activation-induced cytokine; TNFSF11; TRANCE; TRANCEODFhRANKL2; tumor necrosis factor (ligand) superfamily, member 11; tumor necrosis factor ligand superfamily member 11 Tnfsf11|Ly10, Ly109l, O, OD, ODF, OPGL, R, RANKL, Tra, Trance|tumor necrosis factor (ligand) superfamily, member 11|tumor necrosis factor ligand superfamily member 11|OPG ligand|TNF-related activation-induced cytokine|osteoclast differentiation factor|osteoprotegerin ligand|receptor activator of NF-kappaB ligand|receptor activator of nuclear factor kappa-B ligand|tumor necrosis factor-related activation-induced cytokine*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Tnfsf11, check out the Tnfsf11 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Tnfsf11: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
EK0843 has been cited in 12 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Liu Hui,Zhou Chuankun,Qi Dahu,Gao Yutong,Zhu Meipeng,Tao Tenghui,Sun Xuying,Xiao Jun,Inhibiting Monoacylglycerol Lipase Suppresses RANKL-Induced Osteoclastogenesis and Alleviates Ovariectomy-Induced Bone Loss.Frontiers in Cell and Developmental Biology,VOLUME=9,YEAR=2021, PAGES=544, URL=https://www.frontiersin.org/article/10.3389/fcell.2021.640867. DOI=10.3389/fcell.2021.640867,ISSN=2296-634X
EK0843 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA
REV-ERB agonism suppresses osteoclastogenesis and prevents ovariectomy-induced bone loss partially via FABP4 upregulation
An in vitro 3D bone metastasis model by using a human bone tissue culture and human sex-related cancer cells
Amlexanox Suppresses Osteoclastogenesis and Prevents Ovariectomy-Induced Bone Loss
Zhao L, Cai C, Wang J, Zhao L, Li W, Liu C, Guan H, Zhu Y, Xiao J. Front Pharmacol. 2017 Dec 19;8:928. doi: 10.3389/fphar.2017.00928. eCollection 2017. Dihydromyricetin Protects against Bone Loss in Ovariectomized Mice by Suppressing Osteoclast Ac...
Guo J, Li W, Wu Y, Jing X, Huang J, Zhang J, Xiang W, Ren R, Lv Z, Xiao J, Guo F. Front Pharmacol. 2017 Oct 4;8:693. doi: 10.3389/fphar.2017.00693. eCollection 2017. Meclizine Prevents Ovariectomy-Induced Bone Loss and Inhibits Osteoclastogenesis ...
Tang Y, Sun F, Li X, Zhou Y, Yin S, Zhou X. J Endod. 2011 Dec;37(12):1653-8. Doi: 10.1016/J.Joen.2011.08.015. Epub 2011 Oct 2. Porphyromonas Endodontalis Lipopolysaccharides Induce Rankl By Mouse Osteoblast In A Way Different From That Of Escheric...
Zhao H, Ning Ll, Wang Zy, Li Ht, Qiao D, Yao Y, Qin Hl. Cell Biochem Biophys. 2014 Nov;70(2):1097-104. Doi: 10.1007/S12013-014-0028-Z. Calcitonin Gene-Related Peptide Inhibits Osteolytic Factors Induced By Osteoblast In Co-Culture System With Brea...
Tang Zn, Zhang F, Tang P, Qi Xw, Jiang J. Biochem Biophys Res Commun. 2011 May 13;408(3):411-6. Doi: 10.1016/J.Bbrc.2011.04.035. Epub 2011 Apr 13. Hypoxia Induces Rank And Rankl Expression By Activating Hif-1?? In Breast Cancer Cells.
Montesi M, Panseri S, Iafisco M, Adamiano A, Tampieri A. Plos One. 2015 Jul 6;10(7):E0132633. Doi: 10.1371/Journal.Pone.0132633. Ecollection 2015. Coupling Hydroxyapatite Nanocrystals With Lactoferrin As A Promising Strategy To Fine Regulate Bone ...
Xu S, Wang Y, Lu J, Xu J. Rheumatol Int. 2012 Nov;32(11):3397-403. Doi: 10.1007/S00296-011-2175-5. Epub 2011 Nov 6. Osteoprotegerin And Rankl In The Pathogenesis Of Rheumatoid Arthritis-Induced Osteoporosis.
Chen B, Li Xd, Liu Dx, Wang H, Xie P, Liu Zy, Hou Gq, Chang B, Du Sx. Phytomedicine. 2012 Aug 15;19(11):1029-34. Doi: 10.1016/J.Phymed.2012.06.002. Epub 2012 Jul 19. Canonical Wnt Signaling Is Required For Panax Notoginseng Saponin-Mediated Attenu...
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