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Product Info Summary
| SKU: | EK1522 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Mouse TREML1/Tlt 1 ELISA Kit PicoKine®
View all TREML1/TLT-1 ELISA kits
SKU/Catalog Number
EK1522
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse TREML1/Tlt 1 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Treml1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse TREML1/Tlt 1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1522)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: D21-P175
Sensitivity
<10 pg/ml
Assay Range
15.6 pg/ml - 1,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1522 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1522 is reactive to Treml1 in Mouse samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK1522 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of TREML1/TLT-1
Trem-like transcript 1 protein is a protein that in humans is encoded by the TREML1 gene. This gene encodes a member of the triggering receptor expressed on myeloid cells-like (TREM) family. The encoded protein is a type 1 single Ig domain orphan receptor localized to the alpha-granule membranes of platelets. This protein is involved in platelet aggregation, inflammation, and cellular activation and has been linked to Gray platelet syndrome. Alternative splicing results in multiple transcript variants.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1522-CAP | Anti-Mouse Treml1 Pre-coated 96-well strip microplate | 1 |
| EK1522-ST | Mouse Treml1 Standard | 2 vials, 10 ng/tube |
| EK1522-DA | Mouse Treml1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
| O.D. | 0.048 | 0.098 | 0.148 | 0.231 | 0.430 | 0.922 | 1.620 | 2.022 |
Data Example Images
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Mouse TREML1 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect TREML1/TLT-1, Dilution ratio of 1:20000, concentration in serum and plasma is 2.9-4.8 ug/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse TREML1/Tlt 1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 27 | 174 | 493 | 28 | 171 | 489 |
| Standard deviation | 1.4 | 12.35 | 26.12 | 1.54 | 12.99 | 33.74 |
| CV (%) | 5.2% | 7.1% | 5.3% | 5.5% | 7.6% | 6.9% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of TREML1/TLT-1 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 27 | 32 | 29 | 32 | 30 | 2.12 | 7% |
| Sample 2 | 174 | 176 | 207 | 204 | 190 | 15.3 | 8% |
| Sample 3 | 493 | 464 | 486 | 477 | 480 | 10.83 | 2.2% |
Protein Target Info & Infographic
Gene/Protein Information For Treml1 (Source: Uniprot.Org, NCBI)
Gene Name
Treml1
Full Name
Trem-like transcript 1 protein
Weight
33.522kDa
Alternative Names
dJ238O23.3; GLTL1825; PRO3438; TLT1; TLT-1; TLT1MGC119173; TREML1; trem-like transcript 1 protein; triggering receptor expressed on myeloid cells-like 1; Triggering receptor expressed on myeloid cells-like protein 1 Treml1|5430401J17Rik, TLT-, TLT-1|triggering receptor expressed on myeloid cells-like 1|trem-like transcript 1 protein|triggering receptor expressed on myeloid cells-like 1c|triggering receptor expressed on myeloid cells-like 1d|triggering receptor expressed on myeloid cells-like 1e|triggering receptor expressed on myeloid cells-like protein 1
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Treml1, check out the Treml1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Treml1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse TREML1/Tlt 1 ELISA Kit PicoKine® (EK1522)
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No publications found for EK1522
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Mouse TREML1/Tlt 1 ELISA Kit PicoKine®
Question
Q: can I use citrate plasma as samples in Mouse TREML1 Picokine® ELISA Kit (Catalog # EK1522)?
M. Zhou
Verified customer
Asked: 2020-10-26
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of TREML1 causing disruption of its protein structure. TREML1 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the TREML1 ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2020-10-26
Question
Q: What is the optimal O.D. value for TREML1 ELISA kit? I used your TREML1 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain TREML1 even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2019-06-17
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. in your example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this TREML1 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of TREML1 that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2019-06-17
Question
Q: if the enzyme conjugated TREML1 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the TREML1 antigen?
W. Green
Verified customer
Asked: 2018-09-17
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2018-09-17
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
W. Babu
Verified customer
Asked: 2018-05-08
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2018-05-08
Question
Q: how to proceed with the analysis of ELISA data? I measured TREML1 level in plasma.
Verified Customer
Verified customer
Asked: 2017-08-30
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2017-08-30
Question
Q: Can TREML1 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2014-03-14
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is needed. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2014-03-14
