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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).|
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Mouse VCAM-1 ELISA Kit PicoKine™
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Mouse VCAM-1 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Vcam1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 5pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Cite This Product
Mouse VCAM-1 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0538)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: F25-E698
156 pg/ml - 10,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK0538 is reactive to Vcam1 in Mouse samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
EK0538 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
|EK0538-CAP||Anti-Mouse Vcam1 Pre-coated 96-well strip microplate||1|
|EK0538-ST||Mouse Vcam1 Standard||2 vials, 10 ng/tube|
|EK0538-DA||Mouse Vcam1 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Mouse VCAM-1 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect VCAM-1/CD106, Dilution ratio of 1:500, concentration in serum is 960-1200ng/ml.
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse VCAM-1 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of VCAM-1/CD106 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For Vcam1 (Source: Uniprot.Org, NCBI)
Vascular cell adhesion protein 1
CD106 antigen; CD106; DKFZp779G2333; INCAM-100; L1CAM; MGC99561; vascular cell adhesion molecule 1; vascular cell adhesion protein 1; V-CAM 1; VCAM1; VCAM-1 Vcam1|CD106, Vcam, Vcam-1|vascular cell adhesion molecule 1|vascular cell adhesion protein 1|V-CAM 1*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Vcam1, check out the Vcam1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Vcam1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
EK0538 has been cited in 9 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Fu DN,Wang Y,Yu LJ, Liu MJ,Zhen D.Silenced long non-coding RNA activated by DNA damage elevates microRNA-495-3p to suppress atherosclerotic plaque formation via reducing Krüppel-like factor 5.Exp Cell Res.2021 Feb 23: 112519.doi:10.1016/j.yexcr.2021.11251
EK0538 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA
Sun HJ, Xiong SP, Cao X, Cao L, Zhu MY, Wu ZY, Bian JS. Polysulfide-mediated sulfhydration of SIRT1 prevents diabetic nephropathy by suppressing phosphorylation and acetylation of p65 NF-κB and STAT3. Redox Biol. 2020 Nov 25;38:101813. doi: 10.1016/j.redo
Species: Mouse, Human
EK0538 usage in article: APP:ELISA, SAMPLE:RENAL TISSUE, DILUTION:NA
Protective Effect of Irisin on Atherosclerosis via Suppressing Oxidized Low Density Lipoprotein Induced Vascular Inflammation and Endothelial Dysfunction
Apple Polyphenols Decrease Atherosclerosis and Hepatic Steatosis in ApoE?/? Mice through the ROS/MAPK/NF-?B Pathway
Denkovskij, J., Bagdonas, E., Kusleviciute, I., Mackiewicz, Z., Unguryte, A., Porvaneckas, N.,..., & Bernotiene, E. (2017). Paracrine Potential of the Human Adipose Tissue-Derived Stem Cells to Modulate Balance between Matrix Metalloproteinases an...
Asgary S, Keshvari M, Afshani Mr, Amiri M, Laher I, Javanmard Sh. Isrn Nutr. 2014 Mar 4;2014:405867. Doi: 10.1155/2014/405867. Ecollection 2014. Effect Of Fresh Orange Juice Intake On Physiological Characteristics In Healthy Volunteers.
Asgary S, Kelishadi R, Rafieian-Kopaei M, Najafi S, Najafi M, Sahebkar A. Pediatr Cardiol. 2013 Oct;34(7):1729-35. Doi: 10.1007/S00246-013-0693-5. Epub 2013 Apr 27. Investigation Of The Lipid-Modifying And Antiinflammatory Effects Of Cornus Mas L....
Barros Af, Borges Na, Ferreira Dc, Carmo Fl, Rosado As, Fouque D, Mafra D. Future Microbiol. 2015;10(4):517-26. Doi: 10.2217/Fmb.14.140. Is There Interaction Between Gut Microbial Profile And Cardiovascular Risk In Chronic Kidney Disease Patients?
Huang Wy, Wang J, Liu Ym, Zheng Qs, Li Cy. Eur J Pharmacol. 2014 Jan 15;723:67-72. Doi: 10.1016/J.Ejphar.2013.11.041. Epub 2013 Dec 11. Inhibitory Effect Of Malvidin On Tnf-??-Induced Inflammatory Response In Endothelial Cells.
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