Mouse VCAM-1 ELISA Kit PicoKine™

Mouse VCAM-1 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Vcam1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 5pg/ml. Cited in 9 publication(s).

Product Info Summary

SKU: EK0538
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Mouse VCAM-1 ELISA Kit PicoKine™

See all VCAM-1/CD106 products

SKU/Catalog Number

EK0538

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse VCAM-1 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Vcam1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 5pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Mouse VCAM-1 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0538)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: F25-E698

Sensitivity

<5 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0538 is reactive to Vcam1 in Mouse samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK0538 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0538-CAP Anti-Mouse Vcam1 Pre-coated 96-well strip microplate 1
EK0538-ST Mouse Vcam1 Standard 2 vials, 10 ng/tube
EK0538-DA Mouse Vcam1 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631262512502500500010000
O.D.0.0360.0750.1540.3140.4920.9361.3951.997

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect VCAM-1/CD106, Dilution ratio of 1:500, concentration in serum is 960-1200ng/ml.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse VCAM-1 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)3711198438735412984119
Standard deviation26.8%286.25298.3129.02112.92317.16
CV (%)6.8%7.2%6.8%8.2%8.7%7.7%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of VCAM-1/CD106 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 137134937531735323.026.5%
Sample 21198126911791267122840.313.2%
Sample 343874360434539704265171.264%
*number of samples for each test n=16.

Gene/Protein Information For Vcam1 (Source: Uniprot.Org, NCBI)

Uniprot ID

P29533

Gene ID

22329

Gene Name

Vcam1

Full Name

Vascular cell adhesion protein 1

Weight

81.317kDa

Alternative Names

CD106 antigen; CD106; DKFZp779G2333; INCAM-100; L1CAM; MGC99561; vascular cell adhesion molecule 1; vascular cell adhesion protein 1; V-CAM 1; VCAM1; VCAM-1 Vcam1|CD106, Vcam, Vcam-1|vascular cell adhesion molecule 1|vascular cell adhesion protein 1|V-CAM 1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Vcam1, check out the Vcam1 Infographic

Vcam1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Vcam1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0538 has been cited in 9 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Fu DN,Wang Y,Yu LJ, Liu MJ,Zhen D.Silenced long non-coding RNA activated by DNA damage elevates microRNA-495-3p to suppress atherosclerotic plaque formation via reducing Krüppel-like factor 5.Exp Cell Res.2021 Feb 23: 112519.doi:10.1016/j.yexcr.2021.11251
Species: Mouse
EK0538 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

Sun HJ, Xiong SP, Cao X, Cao L, Zhu MY, Wu ZY, Bian JS. Polysulfide-mediated sulfhydration of SIRT1 prevents diabetic nephropathy by suppressing phosphorylation and acetylation of p65 NF-κB and STAT3. Redox Biol. 2020 Nov 25;38:101813. doi: 10.1016/j.redo
Species: Mouse, Human
EK0538 usage in article: APP:ELISA, SAMPLE:RENAL TISSUE, DILUTION:NA

Protective Effect of Irisin on Atherosclerosis via Suppressing Oxidized Low Density Lipoprotein Induced Vascular Inflammation and Endothelial Dysfunction

Apple Polyphenols Decrease Atherosclerosis and Hepatic Steatosis in ApoE?/? Mice through the ROS/MAPK/NF-?B Pathway

Denkovskij, J., Bagdonas, E., Kusleviciute, I., Mackiewicz, Z., Unguryte, A., Porvaneckas, N.,..., & Bernotiene, E. (2017). Paracrine Potential of the Human Adipose Tissue-Derived Stem Cells to Modulate Balance between Matrix Metalloproteinases an...

Asgary S, Keshvari M, Afshani Mr, Amiri M, Laher I, Javanmard Sh. Isrn Nutr. 2014 Mar 4;2014:405867. Doi: 10.1155/2014/405867. Ecollection 2014. Effect Of Fresh Orange Juice Intake On Physiological Characteristics In Healthy Volunteers.

Asgary S, Kelishadi R, Rafieian-Kopaei M, Najafi S, Najafi M, Sahebkar A. Pediatr Cardiol. 2013 Oct;34(7):1729-35. Doi: 10.1007/S00246-013-0693-5. Epub 2013 Apr 27. Investigation Of The Lipid-Modifying And Antiinflammatory Effects Of Cornus Mas L....

Barros Af, Borges Na, Ferreira Dc, Carmo Fl, Rosado As, Fouque D, Mafra D. Future Microbiol. 2015;10(4):517-26. Doi: 10.2217/Fmb.14.140. Is There Interaction Between Gut Microbial Profile And Cardiovascular Risk In Chronic Kidney Disease Patients?

Huang Wy, Wang J, Liu Ym, Zheng Qs, Li Cy. Eur J Pharmacol. 2014 Jan 15;723:67-72. Doi: 10.1016/J.Ejphar.2013.11.041. Epub 2013 Dec 11. Inhibitory Effect Of Malvidin On Tnf-??-Induced Inflammatory Response In Endothelial Cells.

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7 Customer Q&As for Mouse VCAM-1 ELISA Kit PicoKine™

Question

Q: Can CD106 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

Verified Customer

Verified customer

Asked: 2020-09-10

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2020-09-10

Question

Q: if the enzyme conjugated CD106 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the CD106 antigen?

Verified Customer

Verified customer

Asked: 2020-03-04

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2020-03-04

Question

Q: What is the optimal O.D. value for CD106 ELISA kit? I used your CD106 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain CD106 even though the O.D. values are not very high?

L. Tiwari

Verified customer

Asked: 2019-10-26

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. considering your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this CD106 ELISA kit to have sensitivity of 5pg/ml, that means the minimum amount of CD106 that can be declared/interpreted as positive by the above standard is 5pg/ml.

Boster Scientific Support

Answered: 2019-10-26

Question

Q: can you recommend the dilution ratio of serum samples for detection of CD106 in Mouse serum? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have low serum volumes so we like to dilute as much as possible.

P. Sunder

Verified customer

Asked: 2019-04-08

Answer

A: without knowing the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the CD106 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2019-04-08

Question

Q: how to thaw whole blood sample for CD106 ELISA after freezing?

Verified Customer

Verified customer

Asked: 2019-03-16

Answer

A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test CD106 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2019-03-16

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

N. Jha

Verified customer

Asked: 2016-04-07

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2016-04-07

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2016-03-14

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is a must for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2016-03-14

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