Custom Multiplex ELISA Assay Development

Build a custom Q-Plex™ multiplex ELISA panel around your target biomarkers, sample type, and assay performance requirements.

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When to Consider a Custom Multiplex ELISA Assay

Custom assay development is ideal when a catalog panel does not fully match your biomarker targets, sample type, or research workflow.

No Existing Panel Fits

You need a biomarker combination that is not available as a standard pre-made multiplex ELISA kit.

Limited Sample Volume

You want to measure multiple analytes from limited or precious biological samples.

Specific Sample Needs

Your assay requires optimization for a particular species, matrix, sample type, or application.

Development Support

You need a structured path from feasibility review to optimization, verification, and pilot kit production.

Custom Multiplex ELISA Assays Built to Your Specifications

Top-of-the-line ISO 13485 certified assay development

With decades of experience developing sensitive and specific multiplex arrays for a wide range of applications, Boster can help build a custom multiplex tool to meet your research needs. Our assay developers, engineers, product managers, and specialized quality team support projects from feasibility assessment through assay optimization and production readiness.

Our controlled manufacturing environment allows us to produce specialized multiplex ELISA kits with lyophilized reagents for long-term stability. We can also develop controls used for standard curve generation. Costs for a custom ELISA array vary depending on reagent availability, desired biomarkers, and the time required to develop, optimize, and validate the assay.

Start Your Custom Assay Project

Development Workflow

Boster follows a milestone-based development process to evaluate feasibility, optimize assay conditions, verify performance, and prepare custom kits for pilot production.

1

Initial Feasibility

Review your target biomarkers, sample type, project goals, and assay requirements to determine the best development path.

Request a feasibility assessment

2

Proof of Concept

Qualitative assays built

Reagent functionality and compatibility tested
Antibody and matched pair tested
Foreground-to-background signal determined
Intra-panel cross-reactivity tested

3

Assay Optimization

Calibrator formulation and protocol refinement

Detection system review
Diluent optimization
Referencing standard
Protocol and incubation time optimization
Assay characterization

4

Performance Characterization

Precision
Linearity and recovery
Sensitivity
Cross-reactivity
Backfit
Sample correlation
Interference

5

Design Verification

Iterative design and verification help confirm assay performance before moving toward beta kit production.

Precision verification
Drift assessment
Edge effect review
Sample correlation and interference testing

6

Pilot Production & QC Release

Once development and verification are complete, Boster can support beta kit testing, pilot production, and QC release.

Create production documentation
Prepare plates and reagents
QC testing and release

Get Started on Your Custom Assay

What We Help Optimize

Custom multiplex assay development requires more than combining targets into one panel. Boster helps evaluate compatibility, signal quality, protocol conditions, and key assay performance parameters.

Reagent and Pair Compatibility

Evaluate antibody pair functionality, matched pair performance, intra-panel compatibility, and foreground-to-background signal.

Assay Conditions

Optimize diluent, detection conditions, referencing standards, protocol structure, and incubation times.

Performance Parameters

Characterize precision, sensitivity, linearity, recovery, backfit, sample correlation, cross-reactivity, and interference.

Production Readiness

Support beta kit production, production documentation, plate and reagent preparation, QC testing, and release.

Why Develop Your Custom Multiplex Assay with Boster?

Boster combines Q-Plex™ multiplex ELISA technology with assay development, manufacturing, and quality support to help move custom projects from concept to usable research tools.

Q-Plex™ Platform

Develop multiplex ELISA arrays tailored to your biomarker panel and research application.

Assay Development Expertise

Work with a team experienced in feasibility review, optimization, characterization, and verification.

Controlled Manufacturing

Produce specialized multiplex ELISA kits in a controlled manufacturing environment.

QC and Stability Support

Support lyophilized reagents, long-term stability, production documentation, QC testing, and release.

Start with a Feasibility Assessment

Tell us your target biomarkers, species, sample type, expected sample volume, and desired assay format. Our team will review your project and discuss the best development path for your custom multiplex ELISA assay.

Request a Feasibility Assessment