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Looking to get most data out of limited samples? Boster Bio offers multiplex ELISA service at industry's lowest rates, starting from $1.00 per datapoint with our premade panels.
Boster Bio has been a leading manufacturer of singleplex sandwich ELISA kits
since 1993. As a manufacturer of ELISA compatible antibodies, we can easily customize multiplex
immunoassay panels with hundreds of multiplex-optimized biomarkers. Compared with ELISA which
detects a single analyte at a time, multiplex assays allow for simultaneous detection of multiple
targets from the same sample, saving both time and precious samples. Using our pre-made panels
significantly lowers the cost per analyte.
We can perform both microplate-based and bead-based multiplex assays. We will recommend platforms based on panel availability per your project needs.
For microplate, we use the Q-Plex™ platform, a chemiluminescent sandwich ELISA microarray. This assay measures up to 18 analytes using only 25 µL of samples per well. Click here to read more details
For bead assays, our experienced assay developers can work with most platforms. Click here to read more details
Multiplex immunoassays, such as multiplex ELISA (enzyme-linked immunosorbent assay), allow for the quantification of multiple analytes in a single sample. Multi-protein signatures lead to improved insights in disease mechanisms, diagnostics, and the effects of personalized medicine. They are suitable for robust, high throughput, standardized, and affordable analysis of protein targets and biomarkers. Planar assays and microbead-based assays are utilized as capture technologies in multiplex systems.
As easy as 1, 2, 3.
As low as 25 µL/well.
Also more robust.
Get results in 5-10 days.
Industry leading cost.
It is our project concierges' mission to make your project experience as smooth and memorable as possible. They are subject matter experts who are easily accessible around the clock, always happy to help you solve problems, make recommendations and sort through options.
Quantitative technologies compared
||Quantify multiple proteins|
|Western Blot||· High Sensitivity & specificity||
||Detection of single protein|
||Quantify single protein|
The more the better.
Mulitplex Immunoassays such as the planer multiplex ELISA or microbead based suspension assays allow for the simultaneous detection of multiple analytes in a single well or reaction. Multiplex immunoassays yield a wealth of information on the roles of multiple proteins and other biomolecules in complex biological processes in a single sample, thereby providing clinicians with insight into the identification and assessment of disease progression.
Immunoassays have been widely used in many important areas of pharmaceutical analysis such as diagnosis of diseases, therapeutic drug monitoring, clinical pharmacokinetic and bioequivalence studies in drug discovery and pharmaceutical industries.
Let us know what you need tested and we can provide you a quote.
concierge like service
ELISA kits developed
The planar format includes platforms such as Boster’s Q–Plex™ array whereby high-affinity capture ligands are immobilised discretely on a solid phase, 2D support, usually in a microtiter plate format. The immobilised ligands are subsequently exposed to treatment with the sample and probing with detection antibodies labelled with a reporter system. The suspension format includes platforms such as Luminex™, whereby high-affinity capture ligands are immobilized discretely on fluorescently activated plastic microbeads and mixed with the sample in liquid phase. Subsequent addition of detection antibodies labelled with a reporter dye enables high-resolution analysis of specific fluorescent signal via flow cytometric methods.
The Q-Plex™ multiplex assays utilize a sandwich enzyme immunoassay technique for the measurement of multiple targets. These assays use two different antibodies specific for their respective targets. Analyte specific antibodies are immobilized in specific locations on the array. Samples or calibrators are pipetted into wells, and after washing away any unbound protein, a mixture containing biotinylated analyte specific antibodies is added, completing the sandwich for each specific arrayed analyte. After washing away unbound biotinylated antibody, streptavidin-horseradish peroxidase (SHRP) is added, washed, and the amount of SHRP remaining on each location of the array is proportional to the amount of the target initially captured. The amount of conjugated enzyme on each location of the array is measured with the addition of a chemiluminescent substrate using a Q-Plex plate reader.
Microbead-based assays such as the Luminex xMAP technology, utilize the same sandwich ELISA principle as above to multiplex up to 100 different assays within a single sample. This technique involves distinctly colored bead sets, antibody pairs to specific analytes and reporter dyes. One antibody to a specific analyte is attached to a set of beads with the same color, and the second antibody to the analyte is attached to a fluorescent reporter dye label. The use of different colored beads enables the simultaneous multiplex detection of many other analytes in the same sample. A dual detection flow cytometer or Luminex machine is used to sort out the different assays by bead colors in one channel and determine the analyte concentration by measuring the reporter dye fluorescence in another channel.
The advantage of using this platform is that it gives you the ability for large scale
screening of many analytes against your sample at one time. However one disadvantage of larger
panels may be the loss of resolution for lower expressing markers