Click image to see more details
Product Info Summary
| SKU: | FEK0842 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) |
| Assay Time: | 90 Minutes |
How Quick Picokine(R) ELISA kits work: Get data in 90 minutes
Product info
Product Name
Human TRANCE/RANKL/TNFSF11 PicoKine® Quick ELISA Kit
SKU/Catalog Number
FEK0842
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human TRANCE/RANKL/TNFSF11 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human TRANCE/RANKL/TNFSF11 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)
Cite This Product
Human TRANCE/RANKL/TNFSF11 PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0842)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: G64-D245
Capture/Detection Antibodies
The capture antibody is polyclonal antibody from goat and the detection antibody is polyclonal antibody from goat.
Sensitivity
<10 pg/ml
Assay Range
78 pg/ml - 5,000 pg/ml
Standard Dilution Instructions
See datasheet of FEK0842 for more details
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
FEK0842 is reactive to TNFSF11 in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)
Application Guarantee
FEK0842 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of TNFSF11
Receptor activator of nuclear factor kappa-B ligand(RANKL), also known as tumor necrosis factor ligand superfamily member 11(TNFSF11), is a protein that in humans is encoded by the TNFSF11 gene. This gene encodes a member of the tumor necrosis factor(TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. This gene is mapped to chromosome13q14.11. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. This gene may play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy.
Kit Components
| Catalog number | Description | Quantity | Volume |
|---|---|---|---|
| FEK0842-CAP | Anti-tag Pre-coated 96-well Strip Microplate | 1 | 12 strips of 8 wells |
| EK0842-ST | Human TNFSF11 Standard | 2 | 10 ng/tube |
| FEK0842-A | Human TNFSF11 Antibody A | 1 | 3ml |
| FEK0842-B | Human TNFSF11 Antibody B | 1 | 3ml |
| AR1106-1 | Sample Diluent | 1 | 15ml |
| AR1106-7 | TBS-T Wash Buffer (25x) | 1 | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 1 | 10ml |
| AR1105 | Stop Solution | 1 | 10ml |
| PLA-SEA | Adhesive Plate Sealers | 2 | Piece |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional)
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
- Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 78 | 156 | 312 | 625 | 1,250 | 2,500 | 5,000 |
| O.D. | 0.034 | 0.139 | 0.151 | 0.262 | 0.453 | 0.783 | 1.331 | 2.026 |
Data Example Images
Click image to see more details
Human TNFSF11 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human TRANCE/RANKL/TNFSF11 PicoKine® Quick ELISA Kit within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 117 | 867 | 2244 | 116 | 799 | 2281 |
| Standard deviation | 5.85 | 55.48 | 109.95 | 6.26 | 60.72 | 120.89 |
| CV(%) | 5.0% | 6.4% | 4.9% | 5.4% | 7.6% | 5.3% |
Protein Target Info & Infographic
Gene/Protein Information For TNFSF11 (Source: Uniprot.Org, NCBI)
Gene Name
TNFSF11
Full Name
Weight
35.5 kDa
For more info on TNFSF11, check out the TNFSF11 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for TNFSF11: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us support@bosterbio.com.
Specific Publications For Human TRANCE/RANKL/TNFSF11 PicoKine® Quick ELISA Kit (FEK0842)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
0 Reviews For Human TRANCE/RANKL/TNFSF11 PicoKine® Quick ELISA Kit
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
1 Customer Q&As for Human TRANCE/RANKL/TNFSF11 PicoKine® Quick ELISA Kit
Question
What exactly is the difference between Human TRANCE/RANKL/TNFSF11 PicoKine Quick ELISA Kit (FEK0842) versus Human TNFSF11/RANKL ELISA Kit PicoKine (EK0842)?
Verified customer
Asked: 2022-03-08
Answer
The main difference between FEK0842 and EK0842 is that FEK0842 (Quick ELISA) takes 90 minutes to run the assay while EK0842 takes the standard ~3 hours to run the assay. For FEK0842, the incubation times have been shortened while maintaining the quality of the product.
Boster Scientific Support
Answered: 2022-03-08
