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Rat CD86/B7-2 ELISA Kit PicoKine™
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat CD86/B7-2. 96wells/kit, with removable strips.
Rat CD86/B7-2 ELISA Kit PicoKine™ Info At A Glance
| SKU: | EK0712 |
|---|---|
| Size: | 96wells/kit, with removable strips. |
| Reactivity: | Rat |
| Sample Type: | cell culture supernates and serum. |
| Sample Volume: | 100μl per well |
| Sensitivity: | <10pg/ml |
| Assay Range: | 62.5pg/ml-4000pg/ml |
Overview
| Product Name | Rat CD86/B7-2 ELISA Kit PicoKine™
See all Cd86 primary antibodies, ELISA kits and proteins |
|---|---|
| SKU/Catalog Number | EK0712 |
| Storage & Handling | Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.) |
| Description | Detect Rat B7-2/CD86 with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates and serum. This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. B7-2/CD86 tissue specificity: |
| Cite This Product | Rat CD86/B7-2 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0712) |
Product Specs
| Reactivity/Species | Rat |
|---|---|
| Applications | ELISA
*Our Boster Guarantee covers the use of this product in the above tested applications. **For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab. |
| Sample Type | cell culture supernates and serum. |
| Sensitivity | <10pg/ml |
| Assay Range | 62.5pg/ml-4000pg/ml |
| Immunogen | Expression system for standard: NSO; Immunogen sequence: V29-I250 |
| Antibody Clonalities | Capture antibody|Detection antibody monoclonal antibody from mouse|polyclonal antibody from goat |
| Cross Reactivity | There is no detectable cross-reactivity with other relevant proteins. |
| Pack Size | 96wells/kit, with removable strips. |
Protein Target Info (Source: Uniprot.org)
| Uniprot Id | Q62810 |
|---|---|
| Gene Name | Cd86 |
| Protein Name | T-lymphocyte activation antigen CD86 |
*if product is indicated to react with multiple species, protein info is based on the human gene.
Ontology
| Background | Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. Functions as a hormone. |
|---|
Kit Components And Assay QC Data Details Of Rat CD86/B7-2 ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
| Catalog number | Description | Quantity |
|---|---|---|
| EK0712-CAP | 96-well plate precoated with anti-Rat Cd86 antibody | 1 |
| EK0712-ST | lyophilized recombinant Rat Cd86 standard | 10ng/tube |
| EK0712-DA | biotinylated anti-Rat Cd86 antibody | 130ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 130ul |
| AR1106-1 | sample diluent buffer | 30ml |
| AR1106-2 | antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EK0712 Rat CD86/B7-2 ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
| Concentration(pg/ml) | 0 | 4000 | 2000 | 4000 | 2000 | 4000 | 2000 | 4000 |
| O.D. | 0.058 | 0.135 | 0.208 | 0.365 | 0.614 | 0.986 | 1.727 | 2.367 |
We measured random samples of Rat CD86/B7-2 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 179 | 612 | 1878 | 193 | 658 | 1765 |
| Standard deviation | 10.2 | 44.67 | 110.8 | 13.89 | 51.98 | 112.96 |
| CV(%) | 5.7% | 7.3% | 5.9% | 7.2% | 7.9% | 6.4% |
We measured the performance consistency of Rat CD86/B7-2 ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 179 | 159 | 158 | 184 | 170 | 11.64 | 6.8% |
| Sample 2 | 612 | 691 | 664 | 617 | 646 | 32.96 | 5.1% |
| Sample 3 | 1878 | 1659 | 1901 | 1936 | 1843 | 108.5 | 5.8% |
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
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Domestically in USA and Canada, ships next business day if available in stock.
Note: sample size takes longer to process, average 5-7 days.
Contact Information
| [email protected] | |
| (888) 466-3604 |
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All Boster antibodies and ELISA kits are guaranteed to meet the specifications on the data sheet.
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EK0712
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