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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants and serum.|
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Rat CD86/B7-2 ELISA Kit PicoKine™
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Rat CD86/B7-2 ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Cd86 in cell culture supernatants and serum. Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Rat CD86/B7-2 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0712)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: V29-I250
62.5 pg/ml - 4,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK0712 is reactive to Cd86 in Rat samples
Validated Sample Types
cell culture supernatants and serum.
EK0712 is guaranteed for ELISA in Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
|EK0712-CAP||Anti-Rat Cd86 Pre-coated 96-well strip microplate||1|
|EK0712-ST||Rat Cd86 Standard||2 vials, 10 ng/tube|
|EK0712-DA||Rat Cd86 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Rat CD86/B7-2 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect B7-2/CD86, Dilution ratio of 1:1, concentration in serum is around 100pg/ml.
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Rat CD86/B7-2 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of B7-2/CD86 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For Cd86 (Source: Uniprot.Org, NCBI)
T-lymphocyte activation antigen CD86
Activation B7-2 antigen; B70; B7-2 antigen; B72; B7-2; B-lymphocyte activation antigen B7-2; BU63; CD28 antigen ligand 2; CD28LG2B7-2 antigen); CD86 antigen; CD86 molecule; CD86; CTLA-4 counter-receptor B7.2; FUN-1; LAB72; MGC34413; T-lymphocyte activation antigen CD86 CD86 B7-2, B7.2, B70, CD28LG2, LAB72 CD86 molecule T-lymphocyte activation antigen CD86|B-lymphocyte activation antigen B7-2|BU63|CD86 antigen (CD28 antigen ligand 2, B7-2 antigen)|CTLA-4 counter-receptor B7.2|FUN-1*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Cd86, check out the Cd86 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Cd86: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
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