Rat IL-1 Beta/IL-1F2/IL1B ELISA Kit PicoKine®

IL-1 beta/IL-1F2 ELISA kit for Rat

Rat IL-1 Beta/IL-1F2/IL1B ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Il1b in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 1pg/ml. Cited in 10 publication(s).

Product Info Summary

SKU: EK0393
Size: 96 wells/kit, with removable strips.
Reactive Species: Rat
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Rat IL-1 Beta/IL-1F2/IL1B ELISA Kit PicoKine®

View all IL-1 beta/IL-1F2 ELISA kits

SKU/Catalog Number

EK0393

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Rat IL-1 Beta/IL-1F2/IL1B ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Il1b in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 1pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Rat IL-1 Beta/IL-1F2/IL1B ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0393)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: V117-S268

Sensitivity

<1 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0393 is reactive to Il1b in Rat samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK0393 is guaranteed for ELISA in Rat by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of IL-1 beta/IL-1F2

The Interleukin 1 family is a group of 11 beta trefoil cytokines. Interleukin-1, produced mainly by blood monocytes, mediates the panoply of host reactions collectively known as acute phase response . Interleukin-1beta (IL-1beta) is a potent stimulator of bone resorption, and has been implicated in the pathogenesis of high bone turnover and osteoporosis. IL-1beta belongs to proinflammatory cytokines, promote cancer cell adhesion and liver metastases by up-regulating the expression of vascular cell adhesion molecule-1 (VCAM-1) on hepatic sinusoidal endothelium (HSE).

Kit Components

Catalog Number Description Quantity
EK0393-CAP Anti-Rat Il1b Pre-coated 96-well strip microplate 1
EK0393-ST Rat Il1b Standard 2 vials, 10 ng/tube
EK0393-DA Rat Il1b Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)031.262.512525050010002000
O.D.0.0310.0790.1460.2070.3380.5691.0511.829

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect IL-1 beta/IL-1F2, Dilution ratio of 1:1, concentration in serum is target in serum is below detection limit..

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Rat IL-1 Beta/IL-1F2/IL1B ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)5823396262241881
Standard deviation3.6511.4169.264.2112.2968.71
CV (%)7.8%4.9%7.2%6.8%7.8%7.8%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of IL-1 beta/IL-1F2 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 158595756571.111.9%
Sample 223326823725124713.715.5%
Sample 396282692996492056.166.1%
*number of samples for each test n=16.

Gene/Protein Information For Il1b (Source: Uniprot.Org, NCBI)

Uniprot ID

Q63264

Gene ID

24494

Gene Name

Il1b

Full Name

Interleukin-1 beta

Weight

30.644kDa

Superfamily

IL-1 family

Alternative Names

catabolin; IL1 beta; IL-1 beta; IL-1; IL1B; IL-1b; IL1-BETA; IL-1F2; IL1F2IL-1 beta; interleukin 1, beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta Il1b|IL-1F2|interleukin 1 beta|interleukin-1 beta|IL-1 beta

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Il1b, check out the Il1b Infographic

Il1b infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Il1b: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0393 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Salvianolic acid A attenuates early brain injury after subarachnoid hemorrhage in rats by regulating ERK/P38/Nrf2 signaling

Interleukin-1 Receptor-Associated Kinase 1/4 as a Novel Target for Inhibiting Neointimal Formation After Carotid Balloon Injury

Characterization of astrocytes and microglial cells in the hippocampal CA1 region after transient focal cerebral ischemia in rats treated with Ilexonin A

Protective effects of propofol on experimental neonatal acute lung injury

Rapamycin‑induced autophagy attenuates hormone‑imbalance‑induced chronic non‑bacterial prostatitis in rats via the inhibition of NLRP3 inflammasome‑mediated inflammation

‘Psoriasis 1’ reduces psoriasis‑like skin inflammation by inhibiting the VDR‑mediated nuclear NF‑κB and STAT signaling pathways

Allicin attenuates lipopolysaccharide‑induced acute lung injury in neonatal rats via the PI3K/Akt pathway

Activation of the p38 MAPK/NF-κB pathway contributes to doxorubicin-induced inflammation and cytotoxicity in H9c2 cardiac cells

Propofol attenuates inflammatory damage on neurons following cerebral infarction by inhibiting excessive activation of microglia

GPR17 mediates ischemia-like neuronal injury via microglial activation

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12 Customer Q&As for Rat IL-1 Beta/IL-1F2/IL1B ELISA Kit PicoKine®

Question

Q: Can IL-1 beta ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

Verified Customer

Verified customer

Asked: 2020-04-27

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is a must. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2020-04-27

Question

I am interested to purchase the Rat IL-1 Beta ELISA Kit PicoKine antibody, is it suitable to use in rat plasma samples?

Verified Customer

Verified customer

Asked: 2020-04-23

Answer

The product Rat IL-1 Beta ELISA Kit PicoKine antibody would be suitable to with plasma samples.

Boster Scientific Support

Answered: 2020-04-23

Question

Q: how to analyze ELISA data? I have performed IL-1 beta level in plasma.

Verified Customer

Verified customer

Asked: 2020-03-18

Answer

A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2020-03-18

Question

Is there a standardized total protein amount of samples that should be pipetted per well, so that it lies within the assay range of EK0393? Is protein quantification needed?

Verified customer

Asked: 2019-11-05

Answer

For the Rat IL-1 Beta ELISA Kit PicoKine™ (EK0393), add 100ul of diluted sample into each well. It is unnecessary to quantify the amount of total protein.

Boster Scientific Support

Answered: 2019-11-06

Question

What is the recommended dilution for rat serum samples using EK0393?

Verified customer

Asked: 2019-11-05

Answer

The recommended dilution for rat serum samples using the Rat IL-1 Beta ELISA Kit PicoKine™ (EK0393) is 1:1.

Boster Scientific Support

Answered: 2019-11-05

Question

Is it possible to use plasma with heparin in the analyses of the EK0393?

Verified customer

Asked: 2019-10-24

Answer

The Rat IL-1 Beta ELISA Kit PicoKine EK0393 has been validated with plasma with heparin.

Boster Scientific Support

Answered: 2019-10-24

Question

Q: if the enzyme conjugated IL-1 beta antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the IL-1 beta antigen?

D. Tiwari

Verified customer

Asked: 2018-11-29

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2018-11-29

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2018-06-03

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2018-06-03

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2017-08-19

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2017-08-19

Question

Q: how to thaw whole blood sample for IL-1 beta ELISA after freezing?

B. Hernandez

Verified customer

Asked: 2017-07-31

Answer

A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test IL-1 beta for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2017-07-31

Question

Q: is it okay to use citrate plasma as samples in Rat IL-1 beta Picokine® ELISA Kit (Catalog # EK0393)?

Verified Customer

Verified customer

Asked: 2016-08-15

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of IL-1 beta causing disruption of its protein structure. IL-1 beta may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the IL-1 beta ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2016-08-15

Question

Q: how are cell lysates prepared for use in Picokine® ELISA kits?

D. Martin

Verified customer

Asked: 2015-10-29

Answer

A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer may impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2015-10-29

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