SKU EK0393
Size 96wells/kit, with removable strips.
Reactivity Rat
Sample Type cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).
Sensitivity <1pg/ml
Assay Range 31.2pg/ml-2000pg/ml

Overview

Product Name Rat IL-1 Beta PicoKine™ ELISA Kit
SKU/Catalog Number EK0393
Storage & Handling Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Size 96wells/kit, with removable strips.
Description Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat IL-1 beta. 96wells/kit, with removable strips.
Cite This Product Rat IL-1 Beta PicoKine™ ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0393)
Sample Type cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).. Anticoagulant(s): heparin or EDTA
*The recommended anticoagulants are proven to not block the antibody binding sites on the target antigen. Please do not collect blood sample with other anticoagulants thata are not specified above or contact us to check for feasibility.
Sensitivity <1pg/ml
Assay Range 31.2pg/ml-2000pg/ml
Immunogen Expression system for standard: E.coli,V117-S268
Reactivity Rat
Cross Reactivity There is no detectable cross-reactivity with other relevant proteins.

Assay Details

Kit Components

Catalog numberDescriptionQuantity
EK0393-CAP96-well plate precoated with anti-Rat Il1b antibody1
EK0393-STlyophilized recombinant Rat Il1b standard10ng/tube
EK0393-DAbiotinylated anti-Rat Il1b antibody130ul(dilution 1:100)
AR1103Avidin-Biotin-Peroxidase Complex(ABC)130ul(dilution 1:100)
AR1106-1sample diluent buffer30ml
AR1106-2antibody diluent buffer12ml
AR1106-3ABC diluent buffer12ml
AR1104TMB color developing agent10ml
AR1105TMB stop solution10ml
PLA-SEAAdhesive cover4

Materials Required But Not Provided

  • Microplate reader in standard size.
  • Automated plate washer.
  • Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
  • Clean tubes and Eppendorf tubes.
  • Washing buffer (neutral PBS or TBS).
  • Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
  • Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.

Typical Data Obtained from Rat IL-1 Beta PicoKine™ ELISA Kit

(TMB reaction incubate at 37°C for 15-20min)

Concentration(pg/ml)031.262.512525050010002000
O.D.0.1210.1720.1970.2940.4660.7751.3582.167

Intra/Inter Assay Precision

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean(pg/ml)5823396262241881
Standard deviation3.6511.4169.264.2112.2968.71
CV(%)7.8%%%4.9%7.2%6.8%7.8%%7.8%

Reproducibility

Three samples with differing target protein concentrations were assayed using four different lots to measure the CV% lot to lot variance.

Reproducibility

To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.

LotsLot1 (pg/ml)Lot2 (pg/ml)Lot3 (pg/ml)Lot4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 158595756571.111.9%
Sample 223326823725124713.715.5%
Sample 396282692996492056.166.1%
*number of samples for each test n=16.

*The typical data is obtained from Boster's internal QC result and for reference only. It may differ from the lab test results of the end users. It is more important that the user's lab test results reflect the same linearity demonstrated in the typical data than achieving exactly the same O.D. values.

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id Q5BKB0
Gene Name Il1b
Protein Name interleukin 1, beta
Alternative Names Interleukin 1 beta ;Interleukin-1 beta ;Il1b ;rCG_27107 ;
Molecular Weight 30659 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. .
Background The Interleukin 1 family is a group of 11 beta trefoil cytokines. Interleukin-1, produced mainly by blood monocytes, mediates the panoply of host reactions collectively known as acute phase response . Interleukin-1beta(IL-1beta) is a potent stimulator of bone resorption, and has been implicated in the pathogenesis of high bone turnover and osteoporosis. IL-1beta belongs to proinflammatory cytokines, promote cancer cell adhesion and liver metastases by up-regulating the expression of vascular cell adhesion molecule-1(VCAM-1) on hepatic sinusoidal endothelium(HSE).

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Publications

Translational potential of ginsenoside Rb1 in managing progression of osteoarthritis
Theaflavin alleviates inflammatory response and brain injury induced by cerebral hemorrhage via inhibiting the nuclear transcription factor kappa ?-related pathway in rats
3-(1H-Benzo[d]imidazol-6-yl)-5-(4-fluorophenyl)-1,2,4-oxadiazole (DDO7232), a Novel Potent Nrf2/ARE Inducer, Ameliorates DSS-Induced Murine Colitis and Protects NCM460 Cells against Oxidative Stress via ERK1/2 Phosphorylation
Valproic acid attenuates traumatic spinal cord injury-induced inflammation via STAT1 and NF-?B pathway dependent of HDAC3
Omega-3 polyunsaturated fatty acid attenuates the inflammatory response by modulating microglia polarization through SIRT1-mediated deacetylation of the HMGB1/NF-?B pathway following experimental traumatic brain injury
Lentivirus-mediated overexpression of OTULIN ameliorates microglia activation and neuroinflammation by depressing the activation of the NF-?B signaling pathway in cerebral ischemia/reperfusion rats
Pterostilbene protects against acute renal ischemia reperfusion injury and inhibits oxidative stress, inducible nitric oxide synthase expression and inflammation in rats via the Toll-like receptor 4/nuclear factor-?B signaling pathway
Hyperbaric oxygen protects against myocardial reperfusion injury via the inhibition of inflammation and the modulation of autophagy
Salvianolic acid A attenuates early brain injury after subarachnoid hemorrhage in rats by regulating ERK/P38/Nrf2 signaling.
Effects of low-intensity pulsed ultrasound (LIPUS)-pretreated human amnion-derived mesenchymal stem cell (hAD-MSC) transplantation on primary ovarian insufficiency in rats
SOCS2 overexpression alleviates diabetic nephropathy in rats by inhibiting the TLR4/NF-?B pathway
Artesunate prevents rats from the clozapine-induced hepatic steatosis and elevation in plasma triglycerides
NLRP3 inflammasome activation contributes to VSMC phenotypic transformation and proliferation in hypertension
Potassium 2-(1-hydroxypentyl)-benzoate attenuates neuronal apoptosis in neuron?astrocyte co-culture system through neurotrophy and neuroinflammation pathway
Evaluation of the central and peripheral effects of doxepin on carrageenan-induced inflammatory paw edema in rat
Kindlin-1 is a key protein in hyperbaric oxygen therapy for the treatment of neuropathic pain
Omega-3 polyunsaturated fatty acid supplementation attenuates microglial-induced inflammation by inhibiting the HMGB1/TLR4/NF-?B pathway following experimental traumatic brain injury
Picroside II Shows Protective Functions for Severe Acute Pancreatitis in Rats by Preventing NF-?B-Dependent Autophagy
Evaluation of central and peripheral effects of doxepin on acetic acid-induced colitis in rat and the involved mechanisms
Silencing of TRB3 Ameliorates Diabetic Tubule Interstitial Nephropathy via PI3K/AKT Signaling in Rats
Inhibition of BTK protects lungs from trauma-hemorrhagic shock-induced injury in rats
Resveratrol protects against early polymicrobial sepsis-induced acute kidney injury through inhibiting endoplasmic reticulum stress-activated NF-?B pathway
Dusuqing granules (DSQ) suppress inflammation in Klebsiella pneumonia rat via NF-?B/MAPK signaling
Parecoxib reduced ventilation induced lung injury in acute respiratory distress syndrome
Hyperbaric Oxygen Pretreatment Improves Cognition and Reduces Hippocampal Damage Via p38 Mitogen-Activated Protein Kinase in a Rat Model
Suppressing Syndecan-1 Shedding Ameliorates Intestinal Epithelial Inflammation through Inhibiting NF-?B Pathway and TNF-?
Sequential Treatments with Tongsai and Bufei Yishen Granules Reduce Inflammation and Improve Pulmonary Function in Acute Exacerbation-Risk Window of Chronic Obstructive Pulmonary Disease in Rats
Exposure to nickel oxide nanoparticles induces pulmonary inflammation through NLRP3 inflammasome activation in rats
The neutrophil elastase inhibitor, sivelestat, attenuates sepsis-related kidney injury in rats
Inhibitory Effects of Angelica Polysaccharide on Activation of Mast Cells
Antioxidant and Anti-Inflammatory Effects of Coenzyme Q10 on L-Arginine-Induced Acute Pancreatitis in Rat
Wound healing treatment by high frequency ultrasound, microcurrent, and combined therapy modifies the immune response in rats
Decrease of CD68 Synovial Macrophages in Celastrol Treated Arthritic Rats
Preconditioning with VEGF Enhances Angiogenic and Neuroprotective Effects of Bone Marrow Mononuclear Cell Transplantation in a Rat Model of Chronic Cerebral Hypoperfusion
The effect of curcumin and its nanoformulation on adjuvant-induced arthritis in rats
Chronic NF-?B blockade improves renal angiotensin II type 1 receptor functions and reduces blood pressure in Zucker diabetic rats
Methionine Sulfoxide Reductase A Negatively Controls Microglia-Mediated Neuroinflammation via Inhibiting ROS/MAPKs/NF-?B Signaling Pathways Through a Catalytic Antioxidant Function
Epinephrine Enhances the Response of Macrophages under LPS Stimulation
Puerarin partly counteracts the inflammatory response after cerebral ischemia/reperfusion via activating the cholinergic anti-inflammatory pathway
Treatment of acute lung injury by targeting MG53-mediated cell membrane repair
Ginsenoside Rg1 Prevents Cognitive Impairment and Hippocampus Senescence in a Rat Model of D-Galactose-Induced Aging
Effects and Mechanism of Bufei Yishen Formula in a Rat Chronic Obstructive Pulmonary Disease Model
The Protective Effects of Shen-Fu Injection on Experimental Acute Pancreatitis in a Rat Model
Does Quercetin Improve Cardiovascular Risk factors and Inflammatory Biomarkers in Women with Type 2 Diabetes: A Double-blind Randomized Controlled Clinical Trial
Brain microglia were activated in sporadic CJD but almost unchanged in fatal familial insomnia and G114V genetic CJD
Effects of Extract from Mangifera indica Leaf on Monosodium Urate Crystal-Induced Gouty Arthritis in Rats
Yang S, Zhang J, Wang S, Zhao X, Shi J. Oncotarget. 2017 Aug 24;8(53):91185-91198. doi: 10.18632/oncotarget.20434. eCollection 2017 Oct 31. SOCS2 overexpression alleviates diabetic nephropathy in rats by inhibiting the TLR4/NF-κB pathway
Gao D, Jing S, Zhang Q, Wu G. Exp Ther Med. 2018 Jan;15(1):1029-1035. doi: 10.3892/etm.2017.5479. Epub 2017 Nov 10. Pterostilbene protects against acute renal ischemia reperfusion injury and inhibits oxidative stress, inducible nitric oxide synthase expression and inflammation in rats via the Toll-like receptor 4/nuclear factor-κB signaling pathway
Sun HJ, Ren XS, Xiong XQ, Chen YZ, Zhao MX, Wang JJ, Zhou YB, Han Y, Chen Q, Li YH, Kang YM, Zhu GQ. Cell Death Dis. 2017 Oct 5;8(10):e3074. doi: 10.1038/cddis.2017.470. NLRP3 inflammasome activation contributes to VSMC phenotypic transformation and proliferation in hypertension
Chen C, Chen W, Li Y, Dong Y, Teng X, Nong Z, Pan X, Lv L, Gao Y, Wu G. Oncotarget. 2017 Dec 4;8(67):111522-111534. doi: 10.18632/oncotarget.22869. eCollection 2017 Dec 19. Hyperbaric oxygen protects against myocardial reperfusion injury via the inhibition of inflammation and the modulation of autophagy
Gu X, Zheng C, Zheng Q, Chen S, Li W, Shang Z, Zhang H. Am J Transl Res. 2017 Dec 15;9(12):5643-5652. eCollection 2017. Salvianolic acid A attenuates early brain injury after subarachnoid hemorrhage in rats by regulating ERK/P38/Nrf2 signaling.
Lin X, Ju YN, Gao W, Li DM, Guo CC. Biomed Res Int. 2018 Jan 29;2018:7507314. doi: 10.1155/2018/7507314. eCollection 2018. Desflurane Attenuates Ventilator-Induced Lung Injury in Rats with Acute Respiratory Distress Syndrome
Zhu R, Guo W, Fang H, Cao S, Yan B, Chen S, Zhang K, Zhang S. Mol Med Rep. 2018 May;17(5):6357-6362. doi: 10.3892/mmr.2018.8646. Epub 2018 Feb 27. Kupffer cell depletion by gadolinium chloride aggravates liver injury after brain death in rats
Chen X, Chen C, Fan S, Wu S, Yang F, Fang Z, Fu H, Li Y. J Neuroinflammation. 2018 Apr 20;15(1):116. doi: 10.1186/s12974-018-1151-3. Omega-3 polyunsaturated fatty acid attenuates the inflammatory response by modulating microglia polarization through SIRT1-mediated deacetylation of the HMGB1/NF-κB pathway following experimental traumatic brain injury
Zabihi M, Hajhashemi V, Talebi A, Minaiyan M. EXCLI J. 2017 Mar 30;16:414-425. doi: 10.17179/excli2016-727. eCollection 2017. Evaluation of central and peripheral effects of doxepin on acetic acid-induced colitis in rat and the involved mechanisms
Piao X, Liu B, Guo L, Meng F, Gao L. Oxid Med Cell Longev. 2017;2017:7085709. doi: 10.1155/2017/7085709. Epub 2017 Jun 21. Picroside II Shows Protective Functions for Severe Acute Pancreatitis in Rats by Preventing NF-κB-Dependent Autophagy
Li Y, Su R, Xu S, Huang Q, Xu H. Neuropsychiatr Dis Treat. 2017 Sep 27;13:2477-2487. doi: 10.2147/NDT.S145069. eCollection 2017. Artesunate prevents rats from the clozapine-induced hepatic steatosis and elevation in plasma triglycerides
Zabihi M, Hajhashemi V, Minaiyan M, Talebi A. Res Pharm Sci. 2017 Aug;12(4):337-345. doi: 10.4103/1735-5362.212052. Evaluation of the central and peripheral effects of doxepin on carrageenan-induced inflammatory paw edema in rat
Ling L, Feng X, Wei T, Wang Y, Wang Y, Zhang W, He L, Wang Z, Zeng Q, Xiong Z. Stem Cell Res Ther. 2017 Dec 19;8(1):283. doi: 10.1186/s13287-017-0739-3. Effects of low-intensity pulsed ultrasound (LIPUS)-pretreated human amnion-derived mesenchymal stem cell (hAD-MSC) transplantation on primary ovarian insufficiency in rats
Chen Q, Wu H, Tao J, Liu C, Deng Z, Liu Y, Chen G, Liu B, Xu C. PLoS One. 2017 Aug 23;12(8):e0183688. doi: 10.1371/journal.pone.0183688. eCollection 2017. Effect of naringin on gp120-induced injury mediated by P2X7 receptors in rat primary cultured microglia
Liu X, Zhang J, Han W, Wang Y, Liu Y, Zhang Y, Zhou D, Xiang L. Mol Med Rep. 2017 Jul;16(1):192-200. doi: 10.3892/mmr.2017.6553. Epub 2017 May 9. Inhibition of BTK protects lungs from trauma-hemorrhagic shock-induced injury in rats
Chen X, Wu S, Chen C, Xie B, Fang Z, Hu W, Chen J, Fu H, He H. J Neuroinflammation. 2017 Jul 24;14(1):143. doi: 10.1186/s12974-017-0917-3. Omega-3 polyunsaturated fatty acid supplementation attenuates microglial-induced inflammation by inhibiting the HMGB1/TLR4/NF-κB pathway following experimental traumatic brain injury
You C, Zu J, Liu X, Kong P, Song C, Wei R, Zhou C, Wang Y, Yan J. J Cell Mol Med. 2018 Apr;22(4):2449-2457. doi: 10.1111/jcmm.13549. Epub 2018 Jan 30. Synovial fibroblast‐targeting liposomes encapsulating an NF‐κB‐blocking peptide ameliorates zymosan‐induced synovial inflammation
Xu H, Qin W, Hu X, Mu S, Zhu J, Lu W, Luo Y. J Neuroinflammation. 2018 Mar 15;15(1):83. doi: 10.1186/s12974-018-1117-5. Lentivirus-mediated overexpression of OTULIN ameliorates microglia activation and neuroinflammation by depressing the activation of the NF-κB signaling pathway in cerebral ischemia/reperfusion rats
Gao, W., Meng, Q.M., Cui, X.G. (2017). Budesonide instillation immediately after reperfusion ameliorates ischemia/reperfusion-induced injury in the transplanted lung of rat. Experimental Lung Research, 43(9-10), 439-446. doi: 10.1080/01902148.2017.1405103
Vidal, B., Cascão, R., Vale, A.C., Cavaleiro, I., Vaz, M.F., Brito, J.A.,..., & Fonseca, J.E. (2015). Arthritis Induces Early Bone High Turnover, Structural Degradation and Mechanical Weakness. PLOS One, 10(1), e0117100. doi: 10.1371/journal.pone.0117100
Zhao, B., He, E., Pan, Y., Xu, H., & Song, X. (2017). Kindlin-1 is a key protein in hyperbaric oxygen therapy for the treatment of neuropathic pain. Molecular Pain, 13, 1-10. doi: 10.1177/1744806917730254
Yang, B., Gao, P., Wu, X., Yu, J., Li, Y., Meng, R.,..., & Jin, X. (2017). Epigallocatechin‑3‑gallate attenuates neointimal hyperplasia in a rat model of carotid artery injury by inhibition of high mobility group box 1 expression. Experimental and Therapeutic Medicine, 14(3), 1975-1982. doi: 10.3892/etm.2017.4774
Liu, D., Zhang, M., Rong, X., Li, J., &, Wang, X. (2017). Potassium 2-(1-hydroxypentyl)-benzoate attenuates neuronal apoptosis in neuron–astrocyte co-culture system through neurotrophy and neuroinflammation pathway. Acta Pharmaceutica Sinica B. Advance online publication. doi: 10.1016/j.apsb.2017.06.006
Piao, X., Liu, B., Guo, L., Meng, F., & Gao, L. (2017). Picroside II Shows Protective Functions for Severe Acute Pancreatitis in Rats by Preventing NF-κB-Dependent Autophagy. Oxidative Medicine and Cellular Longevity, 1-14. doi: 10.1155/2017/7085709
Sun, G., Yang, W., Zhang, Y., & Zhao, M. (2017). Esculentoside A ameliorates cecal ligation and puncture-induced acute kidney injury in rats. Experimental Animals. Advance online publication. doi: 10.1538/expanim.16-0102
Ma Y, Chen F, Yang S, Duan Y, Sun Z, Shi J. Med Sci Monit. 2017 Jun 10;23:2816-2824. Silencing of TRB3 Ameliorates Diabetic Tubule Interstitial Nephropathy via PI3K/AKT Signaling in Rats
Piao, X., Liu, B., Guo, L., Meng, F., & Gao, L. (2017). Picroside II shows protective functions for severe acute pancreatitis in rats by preventing NF-?B-dependent autophagy. Retrieved from downloads.hindawi.com/journals/omcl/aip/7085709.pdf
Mei X, Wang HX, Li JS, Liu XH, Lu XF, Li Y, Zhang WY, Tian YG. BMC Complement Altern Med. 2017 Apr 17;17(1):216. doi: 10.1186/s12906-017-1736-x. Dusuqing granules (DSQ) suppress inflammation in Klebsiella pneumonia rat via NF-κB/MAPK signaling.
Ghasemi-Pirbaluti M, Motaghi E, Najafi A, Hosseini MJ. Biomed Pharmacother. 2017 Mar 25;90:153-159. doi: 10.1016/j.biopha.2017.03.038. The effect of theophylline on acetic acid induced ulcerative colitis in rats.
Ye J, Caihong S, Yayan H, Zhang X, Meitian X. J Sci Food Agric. 2017 Mar 23. doi: 10.1002/jsfa.8322. Anti-fatigue activity of sea cucumber peptides prepared from Stichopus japonicas in an endurance swimming rat model.

Customer Q&As

Q: Does the TMB color developing agent contain H2O2?
A: Yes, the TMB color developing agent contain H2O2.
Q: Does this ELISA kit contain any product produced in humans or primates?
Keywords: component, ingredient
A: None of the components in our ELISA kits are produced in humans or primates.
Q: What is the volume of the recombinant protein control? What is its shelf life?
Keywords: expiration, storage, temperature, size
A: The volume of the control is around 100pg. If unopened, the shelf life of the control is the same as the whole kit - "Store at 4˚C for 6 months, at -20˚C for 12 months." The reconstituted control can be stored at -20˚C for 2 days.
Q: Will it be okay to run the experiment if I accidentally used the wrong buffer (e.g. sample diluent buffer) for antibody dilution instead of the antibody diluent buffer? Keywords: dilution, replace, substitute, sample buffer
A: Using the sample diluent buffer for antibody dilution instead of the antibody diluent buffer will negatively affect the test result to some extent. The values of both standard and sample might be lower than the normal values.
Q: The protocol says to use neutral PBS and provides a recipe. Could I use neutral PBS made with KCl or KH2PO4 (common constituents for most PBS recipes) instead? Keywords: protocol, alternative buffer, applications, reagent recipe
A: Yes, it is ok to use common PBS recipes. We have tried many types of buffers with common constituents, and no significant difference was observed whatsoever.
Q: Why are my O.D. values different than your values on the datasheet? Keyword: troubleshooting, reference
A: We detected the kit in our lab and got our values on the datasheet before delivery, but protein activity will decrease as time goes on, so you may get lower O.D. values than ours. However, it should still be in a reasonable range and the standards can be used to calculate sample values. Good linearity of curve is more important than the actual numerical value.
Q: On the ELISA kit datasheet it says "HRP substrate TMB was used to visualize HRP enzymatic reaction." What is HRP and which part of the kit contains HRP? Keyword: kit components, reagents, protocol
A: It means Avidin-Biotin-Peroxidase Complex(AR1103) and you could find it in Kit Components.
Q: Do you offer any ELISA kits that can work with whole blood samples instead of plasma or urine? Keyword: applications
A: We test serum and plasma routinely, and there is very little difference between serum, plasma and whole blood. The whole blood also contains proteins we need to test. The kit can be used to test whole blood in theory.
Q: Does silicic acid (formula SiOH4) affect the results of the ELISA assay? Keyword: protocol, reagents
A: The acid may affect the binding of antigen to antibody, it is not recommended to use. That being said, it is unlikely to affect the reaction if the solution remains an overall neutral environment.
Q: Is there lot-to-lot variation of the ELISA kits? What are Boster's general services when I have questions about your kits? Keyword: technical support, help, customer service
A: The NIBSC/WHO 1st International Standard is evaluated, we always test our kits before delivery, and customers can find the test result on the protocol. If the customer needs technical support from us to analyze their data, please contact us and we ask that you provide us the following information: the lot# and production date, and when did the customer detect the kit.
Q: The results of my standards do not look correct, what could be the problem? Keyword: troubleshoot, protocol
A: Double check the protocol for plate washing. It should be examined on three aspects: 1) Did you make the washing buffer correctly? 2) How long did you let the buffer stay in wells? 3) What was the volume of the washing buffer added to each well? In addition, pay attention when adding sample to avoid contamination. And we suggest testing the standards again. Values of standards at low concentration are more affected than that at higher concentration, so customer can still get expected values at high concentration even if there is an error. If problems persist, please contact technical support with the catalog and lot number of your product.
Q: What is the well depth of the 96 well plates for the ELISA kits? Keyword: well capacity, product size
A: The well depth is 300ul, and the max capacity is 350ul. The height of the well is 1.1 cm, and the internal size is 1 cm.
Q: The kit does not include wash solution, what should I do? Keyword: wash buffer
A: Our Elisa kits do not come with wash solution, but we have included information about how to make washing buffer on the datasheet. Please refer to the "Material Required But Not Provided" section. We also offer washing buffer for sale separately (Phosphate Buffered Saline Powder SKU: AR0030).
Q: For your ELISA kits, are the capture and detection antibodies polyclonals or monoclonals? Keyword: antibody clonality
A: This information can be found for each kit under the "Properties" section, and you can find the immunogen sequence information in the "Overview" section.
Q: Do your ELISA kits come with sealants or plate covers? Keyword: storage, sequential use
A: The kit can be used within a month sequentially if it's opened and stored at 4 degree. There is no need to use sealants, the plate can be packaged with aluminum foil bag, and for other reagents keep bottle tightly closed.
Q: What is the concentration of Sodium Azide in your Elisa kits? Keyword: preservative
A: Our Elisa kits contain 0.02% Sodium Azide. All of the components except TMB colour developing reagent and stop solution contains 0.02% Sodium azide as the preservative.
Q: Are there positive and negative controls available for my ELISA kit? Keyword: positive control, negative control
A: We can provide a recombinant protein as a control. It costs $50 per control and takes 2 weeks to manufacture. We cannot provide a positive or negative control in serum.
Q: Why do I get positive results for my knockout (KO) model when used as a control?
A: The knockout (KO) model may contain truncated forms of the target protein which can be detected by the ELISA assay.
Q: How long do I soak my plate in the wash buffer?
A: The plate should soak in 0.3mL PBS or TBS wash buffer for at least 1-2 minutes in an automated wash station.
Q: Can I use Tween in my wash buffer?
A: While it is not recommended to use Tween in your wash buffer, small amounts (<0.1% concentration) may decrease background due to insufficient blocking.
Q: What plate type do I use to set up the microplate reader?
A: Our plates are made with the Corning costar plates similar to the DNA-BIND 96 -well plates.
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the normal level of this protein in my sample of interest?
A: We have reviewed literature and have gathered this information for most of our ELISA kits. You can find this information on the product page or contact us if you cannot find it. However this information is only suggestive and cannot replace pilot studies in determining the optimal sample dilution ratio.
Q: Is the plate separable? Can I use only part of the kit and save the rest for later? How many samples can I run with one kit?
A: Yes the plate is separable. There are 12 strips of 8 wells each, all removable from the plate. The amount of samples you can run depend on a few factors. In the most common ELISA set up, you will use two strips for standards, and 10 strips for samples using duplicates, which let you run up to 40 samples per kit. Contact us if you have questions regarding other situations.
Q: Does the kit contain sample preparation reagents? How do I prepare my samples?
A: Since different sample types require different reagents, we do not include them in the ELISA kit. However we do have each reagent mentioned in the file below available at very reasonable prices. Be sure to check them out. For sample preparation protocols please download the file below: https://www.bosterbio.com/media/pdf/Boster_Sample_Preparation_Protocols.pdf
Q: Can this ELISA kit work on brain tissue homogenate, cell culture supernatant, saliva, urine, serum, whole blood or any other sample type?
A: In theory the ELISA kit will work for all sample types if the target protein is present at a level that falls within the linear range of the ELISA kit detection range. We guarantee the kit to work on the sample types that we have tested. If you want dilution ratio suggestions on these sample types please contact our technical support. For sample types that we have not tested for, we suggest you run pilot experiments to decide the optimal sample dilution.
Q: Can this ELISA kit react with the pro-form of the target protein? Can this ELISA kit react with an isoform of the protein?
A: In general, unless otherwise specified, the ELISA kit is pan-specific, meaning that it will react with all different forms of the target protein if they share the majority of the target protein's sequence. The capture and detection antibodies are reactive to the entire sequence of the standard protein. You can find the sequence information of the standard protein in the "immunogen" section. For more information about the specificity of the kit for your particular experiment, please contact our techincal support.
Q: Can this ELISA kit react with human, mouse, rat or other species?
A: If the kit is reactive to another commonly used species (human, mouse, and/or rat), we would have listed it as a separate product. If you want to check cross-reactivity to a species that is not included in the 3 species listed above, please contact our technical support for more information. As a rule of thumb, if the sequences are 90%+ identical, there is a high chance of cross-reactivity for your species of interest.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to rat il 1b elisa kit, rat il 1 beta elisa kit, rat il 1 elisa kit, rat il1 beta elisa kit, rat il-1 beta elisa kit, rat il1b elisa kit