Rat IL-1 Beta/IL-1F2/IL1B ELISA Kit PicoKine™

Rat IL-1 Beta/IL-1F2/IL1B ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Il1b in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 1pg/ml. Cited in 101 publication(s).

Product Info Summary

SKU: EK0393
Size: 96 wells/kit, with removable strips.
Reactive Species: Rat
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Rat IL-1 Beta/IL-1F2/IL1B ELISA Kit PicoKine™

See all IL-1 beta/IL-1F2 products

SKU/Catalog Number

EK0393

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Rat IL-1 Beta/IL-1F2/IL1B ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Il1b in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 1pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Rat IL-1 Beta/IL-1F2/IL1B ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0393)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: V117-S268

Sensitivity

<1 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0393 is reactive to Il1b in Rat samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK0393 is guaranteed for ELISA in Rat by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0393-CAP Anti-Rat Il1b Pre-coated 96-well strip microplate 1
EK0393-ST Rat Il1b Standard 2 vials, 10 ng/tube
EK0393-DA Rat Il1b Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)031.262.512525050010002000
O.D.0.0310.0790.1460.2070.3380.5691.0511.829

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect IL-1 beta/IL-1F2, Dilution ratio of 1:1, concentration in serum is target in serum is below detection limit..

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Rat IL-1 Beta/IL-1F2/IL1B ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)5823396262241881
Standard deviation3.6511.4169.264.2112.2968.71
CV (%)7.8%4.9%7.2%6.8%7.8%7.8%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of IL-1 beta/IL-1F2 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 158595756571.111.9%
Sample 223326823725124713.715.5%
Sample 396282692996492056.166.1%
*number of samples for each test n=16.

Gene/Protein Information For Il1b (Source: Uniprot.Org, NCBI)

Uniprot ID

Q63264

Gene ID

24494

Gene Name

Il1b

Full Name

Interleukin-1 beta

Weight

30.644kDa

Superfamily

IL-1 family

Alternative Names

catabolin; IL1 beta; IL-1 beta; IL-1; IL1B; IL-1b; IL1-BETA; IL-1F2; IL1F2IL-1 beta; interleukin 1, beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta Il1b|IL-1F2|interleukin 1 beta|interleukin-1 beta|IL-1 beta

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Il1b, check out the Il1b Infographic

Il1b infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Il1b: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0393 has been cited in 101 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Chen Q,Lei YQ,Liu JF,Wang ZC,Cao H.Beneficial effects of chlorogenic acid treatment on neuroinflammation after deep hypothermic circulatory arrest may be mediated through CYLD/NF-κB signaling.Brain Res.2021 Jun 30:147572.doi:10.1016/j.brainres.2021.147572.Epub ahead of print.PMID:34216581.
Species: Rat
EK0393 usage in article: APP:ELISA, SAMPLE:BRAIN TISSUE, DILUTION:NA

Hou,C.,Wang,X.,Jiang,W.,Bian,Z.,Zhu,L.,& Li,M.(2021).Peptide 11R‑VIVIT promotes fracture healing in osteoporotic rats.International Journal of Molecular Medicine,48,162.https://doi.org/10.3892/ijmm.2021.4995
Species: Rat
EK0393 usage in article: APP:ELISA, SAMPLE:BMSCS, DILUTION:NA

Li Q,Jin Y,Ye X,Wang W,Deng G,Zhang X.Bone Marrow Mesenchymal Stem Cell-Derived Exosomal MicroRNA-133a Restrains Myocardial Fibrosis and Epithelial-Mesenchymal Transition in Viral Myocarditis Rats Through Suppressing MAML1.Nanoscale Res Lett.2021 Jul 2;16(1):111.doi:10.1186/s11671-021-03559-2.PMID:34215939.
Species: Rat
EK0393 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

Tang Q,Shang F,Wang X,Yang Y,Chen G,Chen Y,Zhang J,Xu X.Combination use of ferulic acid, ligustrazine and tetrahydropalmatine inhibits the growth of ectopic endometrial tissue: a multi-target therapy for endometriosis rats.J Ethnopharmacol.2014 Feb 12;151(3):1218-1225.doi:10.1016/j.jep.2013.12.047.Epub 2014 Jan 2.PMID: 24389027.
Species: Rat
EK0393 usage in article: APP:ELISA, SAMPLE:MACROPHAGES, DILUTION:NA

Wang Y,Chen S,Tan J,Gao Y,Yan H,Liu Y,Yi S,Xiao Z,Wu H.Tryptophan in the diet ameliorates motor deficits in a rotenone-induced rat Parkinson‘s disease model via activating the aromatic hydrocarbon receptor pathway.Brain Behav.2021 Jun 9.doi:10.1002/brb3.2226.Epub ahead of print.PMID:34105899.
Species: Rat
EK0393 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

Lipopolysaccharides,I.C.I.B.B.,Fesal,M.,Seif,F.,Abdella,A.,& Amin,H.M.Middle East Journal of Applied Sciences Volume:11| Issue: 01|Jan.-March| 2021.
Species: Rat
EK0393 usage in article: APP:ELISA, SAMPLE:LIVER TISSUE, DILUTION:NA

Ming Tang,Xia Gao,Ting Geng,Xialin Chen,Jiajia Wang,Canjie Shen,Huifang Gao,Mengyu Qian,Zhenzhong Wang,Liang Cao,Wei Xiao,Metabolomics analysis of the therapeutic effects of Qiwei Tongbi oral liquid on rheumatoid arthritis in rats,Journal of Pharmaceutical and Biomedical Analysis, Volume 202,2021,114166,ISSN 0731-7085,https://doi.org/10.1016/j.jpba.2021.114166.
Species: Rat
EK0393 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

Effect of α-Tocopherol on Cytokines Production, Oxidative Stress, and Liver Injury Induced by Lipopolysaccharides in Male Albino Rats. Amin,H. M.
Species: Rat
EK0393 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION: NA

Cheng Wang,Liang Xian,Shaorui Zheng et al.Cranial Venous Outflow Obstruction Promotes Neuroinflammation And Edema Via ADAM17/solTNF-α/NF-κB Pathway In A Rat Subdural Hematoma Model,11 May 2021,PREPRINT (Version 1) available at Research Square [https://doi.org/10.21203/rs.3.rs-487715/v1]
Species: Rat
EK0393 usage in article: APP:ELISA, SAMPLE:BRAIN TISSUE, DILUTION:NA

Motavallian A,Bouzari S,Zamani E,Karimian P,Dabirian S,Molavi M,Torshkooh FA.An investigation of the anti-inflammatory effects of gabapentin on acetic acid-induced colitis in rats.Mol Biol Rep.2021 Apr 29.doi:10.1007/s11033-021-06357-2.Epub ahead of print.PMID:33928442.
Species: Rat
EK0393 usage in article: APP:ELISA, SAMPLE:COLON TISSUE, DILUTION:NA

Xu H,Wang Y,Luo Y.OTULIN is a new target of EA treatment in the alleviation of brain injury and glial cell activation via suppression of the NF-κB signalling pathway in acute ischaemic stroke rats.Mol Med.2021 Apr 9;27(1):37.doi:10.1186/s10020-021-00297-0.PMID:33836646;PMCID:PMC8035756.
Species: Rat
EK0393 usage in article: APP:ELISA, SAMPLE:BRAIN TISSUE, DILUTION:NA

Ying-nan Ju,Qi-hang Tai,Guang-xiao Xu,Xiao-qing Zhao,Hai-bin Sun,Wei Gao,"Diannexin Can Ameliorate Acute Respiratory Distress Syndrome in Rats by Promoting Heme Oxygenase-1 Expression",Mediators of Inflammation,vol.2021,Article ID 1946384,10 pages,2021.https://doi.org/10.1155/2021/1946384
Species: Rat
EK0393 usage in article: APP:ELISA, SAMPLE:BALF AND SERUM, DILUTION:NA

Abukhalil MH, Althunibat OY, Aladaileh SH, Al-Amarat W, Obeidat HM, Al-Khawalde AAA, Hussein OE, Alfwuaires MA, Algefare AI, Alanazi KM, Al-Swailmi FK, Arab HH, Mahmoud AM. Galangin attenuates diabetic cardiomyopathy through modulating oxidative stress, inflammation and apoptosis in rats. Biomed Pharmacother. 2021 Mar 19:111410. doi: 10.1016/j.biopha.2021.111410. Epub ahead of print. PMID: 33752930.
Species: Rat
EK0393 usage in article: APP:ELISA, SAMPLE:HEART TISSUE, DILUTION:NA

Elmazoglu Z,Galván-Arzate S,Aschner M,Rangel-López E,Bayraktar O,Santamaría A,Karasu Ç.Redox-active phytoconstituents ameliorate cell damage and inflammation in rat hippocampal neurons exposed to hyperglycemia+Aβ1-42 peptide.Neurochem Int.2021 Feb 19;145:
Species: Rat
EK0393 usage in article: APP:ELISA, SAMPLE:NEURONS, DILUTION:NA

Jia Q,Zhang H,Su Y,Liu X,Bai J,Lang W,Shi Q,Feng M.Strictosamide alleviates the inflammation in an acute ulcerative colitis (UC) model.J Physiol Biochem.2021 Feb 17.doi:10.1007/s13105-021-00796-y.Epub ahead of print.PMID:33595775.
Species: Human,Mouse,Rat
EK0393 usage in article: APP:ELISA, SAMPLE:COLON TISSUE, DILUTION:NA

Hongbei Xu,You Wang,Yong Luo et al. OTULIN is a New Target for EA Treatment in Alleviating Brain Injury and The Activation of Glial Cells By Depressing NF-κB Signalling Pathway in Acute Ischaemic Stroke Rats,28 January 2021,PREPRINT (Version 1) available
Species: Rat
EK0393 usage in article: APP:ELISA, SAMPLE:BRAIN TISSUE, DILUTION:NA

Haiyang Zhang,Wenjing Jiao,Hailin Cui,Qinghong Sun,Honggang Fan,Combined exposure of alumina nanoparticles and chronic stress exacerbates hippocampal neuronal ferroptosis via activating IFN-γ/ASK1/JNK signaling pathway in rats,Journal of Hazardous Materia
Species: Rat
EK0393 usage in article: APP:ELISA, SAMPLE:HIPPOCAMPUS, DILUTION:NA

Manman Zhao,Junling Gao,Changmeng Cui,Yanan Zhang,Xiaohua Jiang,Jianzhong Cui, "Inhibition of PTEN Ameliorates Secondary Hippocampal Injury and Cognitive Deficits after Intracerebral Hemorrhage: Involvement of AKT/FoxO3a/ATG-Mediated Autophagy",Oxidative
Species: Rat
EK0393 usage in article: APP:ELISA, SAMPLE:HIPPOCAMPUL TISSUE, DILUTION:NA

Chen T,Liu WB,Qian X,Xie KL,Wang YH.The AMPAR antagonist perampanel protects the neurovascular unit against traumatic injury via regulating Sirt3.CNS Neurosci Ther.2021 Jan 9.doi:10.1111/cns.13580.Epub ahead of print.PMID:33421349.
Species: Rat
EK0393 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

Liu Yuan,Luo Pei,Zhang Ji-Zhong,Chen Xiao-Yi,Li Li-Min,Wu You-Jiao,Yin Qiao-Zhi,Huang Li.Identification of Active Compounds From Yi Nationality Herbal Formula Wosi Influencing COX-2 and VCAM-1 Signaling. Frontiers in Pharmacology,11.2020.1690.https://www.
Species: Rat
EK0393 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

Jiang,Guoyong and Lei,AItong and Chen,Yi and Yu,Qiang and Xie,Jianhua and Yang,Ying and Yuan,Tongji and Su, Dan", "Protective effects of Ganoderma atrum polysaccharide against acrylamide-induced inflam mation and oxidative damage in rats","Food Funct.","2
Species: Rat
EK0393 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

Yingchao Kang, Yaping Sun, Tiantian Li, Zelin Ren,Garcinol protects against cerebral ischemia-reperfusion injury in vivo and in vitro by inhibiting inflammation and oxidative stress, Molecular and Cellular Probes ,2020, 101672,ISSN 0890-8508,https://doi.o
Species: Rat
EK0393 usage in article: APP:ELISA, SAMPLE:BRAIN TISSUE AND PC12 CELLS, DILUTION:NA

Wang,Q.,Liu,S.,& Han,Z.(2020).miR-339-3p regulated acute pancreatitis induced by caerulein through targeting TNF receptor-associated factor 3 in AR42J cells, Open Life Sciences , 15(1), 912-922. doi:https://doi.org/10.1515/biol-2020-0084.
Species: Rat
EK0393 usage in article: APP:ELISA, SAMPLE:MACROPHAGE, DILUTION:NA

Yudi Zhang,Chunhe Tao,Donglin Du, et al.Astragaloside IV Protects Against Diabetic Nephropathy by Inhibiting Pyroptosis Based on NOX4/TXNIP/NLRP3 Pathway. Authorea. December 01, 2020. DOI: 10.22541/au.160684072.22355285/v1
Species: Rat
EK0393 usage in article: APP:ELISA, SAMPLE:SERUM AND KIDNEY, DILUTION:NA

Zhang K,Liu R,Gao Y,Ma W,Shen W.Electroacupuncture Relieves LPS-Induced Depression-Like Behaviour in Rats Through IDO-Mediated Tryptophan-Degrading Pathway.Neuropsychiatr Dis Treat.2020 Oct 5;16:2257-2266.doi:10.2147/NDT.S274778.PMID:33116524;PMCID:PMC754
Species: Rat
EK0393 usage in article: APP:ELISA, SAMPLE:HIPPOCAMPUS AND SERUM, DILUTION:NA

Li J,Ma J,Tian Y,Zhao P,Liu X,Dong H,Zheng W,Feng S,Zhang L,Wu M,Zhu L,Liu S,Zhao D.Effective-component compatibility of Bufei Yishen formula II inhibits mucus hypersecretion of chronic obstructive pulmonary disease rats by regulating EGFR/PI3K/mTOR signa
Species: Rat
EK0393 usage in article: APP:ELISA, SAMPLE:SERUM AND BALF, DILUTION:NA

Süntar I,Cevik CK,Çeribaşı AO,Gökbulut A. Healing effects of Cornus mas L. in experimentally induced ulcerative colitis in rats: From ethnobotany to pharmacology. J Ethnopharmacol.2020 Feb 10;248:112322. doi:10.1016/j.jep.2019.112322. Epub 2019 Oct 20.PMI
Species: Rat
EK0393 usage in article: APP:ELISA, SAMPLE:COLON TISSUE, DILUTION:NA

Wan SY,Li GS,Tu C,Chen WL,Wang XW,Wang YN,Peng LB,Tan F.MicroNAR-194-5p hinders the activation of NLRP3 inflammasomes and alleviates neuroinflammation during intracerebral hemorrhage by blocking the interaction between TRAF6 and NLRP3.Brain Res.2020 Dec 2
Species: Rat
EK0393 usage in article: APP:ELISA, SAMPLE:BRAIN TISSUE, DILUTION:NA

Fahmy EK,El-Sherbiny M,Said E,Elkattawy HA,Qushawy M,Elsherbiny N.Tranilast ameliorated subchronic silver nanoparticles-induced cerebral toxicity in rats: Effect on TLR4/NLRP3 and Nrf-2. Neurotoxicology.2020 Dec 23;82:167-176.doi:10.1016/j.neuro.2020.12.0
Species: Rat
EK0393 usage in article: APP:ELISA, SAMPLE:BRAIN HOMOGENATE, DILUTION:NA

Liu Y,Zhang X,Yan D,Wang Y,Wang N,Liu Y,Tan A,Chen X,Yan H.Chronic acrylamide exposure induced glia cell activation, NLRP3 infl-ammasome upregulation and cognitive impairment.Toxicol Appl Pharmacol.2020 Apr 15;393:114949.doi:10.1016/j.taap.2020.114949.Epu
Species: Rat
EK0393 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

Fan X,Ma W,Zhang Y,Zhang L.P2X7 Receptor (P2X7R) of Microglia Mediates Neuroinflammation by Regulating (NOD)-Like Receptor Protein 3 (NLRP3) Inflammasome-Dependent Inflammation After Spinal Cord Injury. Med Sci Monit.2020 Sep 21;26:e925491.doi:10.12659/MS
Species: Rat
EK0393 usage in article: APP:ELISA, SAMPLE:SPINAL CORD, DILUTION:NA

Tong L,Tang C,Cai C,Guan X. Upregulation of the microRNA rno-miR-146b-5p may be involved in the development of intestinal injury through inhibition of Kruppel-like factor 4 in intestinal sepsis. Bioengineered. 2020 Nov 17.doi:10.1080/21655979.2020.1851476
Species: Rat
EK0393 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

Lu P,Yang G,Jiang L,He W,Wu W,Qi L,Shen S,Rao J,Zhang P,Xue Z,Jiang C,Fan G,Zhu X. Characterizing disease progression of nonalcoholic steatohepatitis in Leptin-deficient rats by integrated transcriptome analysis. Exp Biol Med (Maywood).2020 Dec 10:1535370
Species: Rat, Mouse
EK0393 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

Liu S, Wang Y, Lu S, Hu J, Zeng X, Liu W, Wang Y, Wang Z. Sacubitril/valsartan treatment relieved the progression of established pulmonary hypertension in rat model and its mechanism. Life Sci. 2020 Dec 10:118877. doi: 10.1016/j.lfs.2020.118877. Epub ahea
Species: Rat
EK0393 usage in article: APP:ELISA, SAMPLE:BLOOD, DILUTION:NA

Li L,Zou Y,Liu B,Yang R,Yang J,Sun M,Li Z,Xu X,Li G,Liu S,Greffrath W,Treede RD,Li G,Liang S. Contribution of the P2X4 Receptor in Rat Hippocampus to the Comorbidity of Chronic Pain and Depression. ACS Chem Neurosci.2020 Dec 7.doi:10.1021/acschemneuro.0c0
Species: Rat
EK0393 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

Zheng X,Cai X,Ye F,Li Y,Wang Q,Zuo Z,Huang W,Wang Z. Perioperative Dexmedetomidine attenuates brain ischemia reperfusion injury possibly via up-regulation of astrocyte Connexin 43. BMC Anesthesiol. 2020 Dec 7;20(1):299.doi: 10.1186/s12871-020-01211-7.PMID
Species: Rat
EK0393 usage in article: APP:EISA, SAMPLE:BRAIN TISSUE, DILUTION:NA

Liu Z,Meng Y,Miao Y,Yu L,Yu Q. Propofol reduces renal ischemia/reperfusion-induced acute lung injury by stimulating sirtuin 1 and inhibiting pyroptosis. Aging(Albany NY).2020 Dec1;12.doi:10.18632/aging.202191.Epub ahead of print.PMID:33260147.
Species: Rat
EK0393 usage in article: APP:ELISA, SAMPLE:NR8383 CELL SUPERNATANTS AND RAT LUNG TISSUE, DILUTION:NA

Xie M,Cheng M,Wang B,Jiao M,Yu L,Zhu H.2-Bromopalmitate attenuates inflammatory pain by maintaining mitochondrial fission/fusion balance and function. Acta Biochim Biophys Sin (Shanghai).2020 Nov 30:gmaa150. doi: 10.1093/abbs/gmaa150.Epub ahead of print.P
Species: Rat
EK0393 usage in article: APP:ELISA, SAMPLE:SPINAL CORD, DILUTION:NA

Wang Q, Xu GX, Tai QH, Wang Y. Lipoxin A4 Reduces Ventilator-Induced Lung Injury in Rats with Large-Volume Mechanical Ventilation. Mediators Inflamm. 2020 Nov 22;2020:6705985. doi: 10.1155/2020/6705985. PMID: 33299377; PMCID: PMC7704204.
Species: Rat
EK0393 usage in article: APP:ELISA, SAMPLE:BALF, DILUTION:NA

Elmazoglu Z, Prnova MS, Santamaria A, Stefek M, Karasu C. Combatting Nitrosative Stress and Inflammation with Novel Substituted Triazinoindole Inhibitors of Aldose Reductase in PC12 Cells Exposed to 6-Hydroxydopamine Plus High Glucose. Neurotox Res. 2020
Species: Rat
EK0393 usage in article: APP:ELISA, SAMPLE:PC12 CELLS, DILUTION:NA

Zhang, B., Guo, Z., Lai, S., & Chen, H. (2020). Interference with miR-210 Alleviated Renal Injury in Septic Rats by Inhibiting JAK-STAT Pathway. Inflammation, 1-10.

Activation of the p38 MAPK/NF-%u03BAB pathway contributes to doxorubicin-induced inflammation and cytotoxicity in H9c2 cardiac cells

Alveolar bone protective and hypoglycemic effects of systemic propolis treatment in experimental periodontitis and diabetes mellitus

Andrographolide alleviates acute brain injury in a rat model of traumatic brain injury: possible involvement of inflammatory signaling

Exogenous H2S protects H9c2 cardiac cells against high glucose-induced injury and inflammation by inhibiting the activation of the NF-%u03BAB and IL-1%u03B2 %u2026

Protection of SAL B with H9C2 cells

Translational potential of ginsenoside Rb1 in managing progression of osteoarthritis

Theaflavin alleviates inflammatory response and brain injury induced by cerebral hemorrhage via inhibiting the nuclear transcription factor kappa ?-related pathway in rats

3-(1H-Benzo[d]imidazol-6-yl)-5-(4-fluorophenyl)-1,2,4-oxadiazole (DDO7232), a Novel Potent Nrf2/ARE Inducer, Ameliorates DSS-Induced Murine Colitis and Protects NCM460 Cells against Oxidative Stress via ERK1/2 Phosphorylation

Valproic acid attenuates traumatic spinal cord injury-induced inflammation via STAT1 and NF-?B pathway dependent of HDAC3

Potassium 2-(1-hydroxypentyl)-benzoate attenuates neuronal apoptosis in neuron?astrocyte co-culture system through neurotrophy and neuroinflammation pathway

Resveratrol protects against early polymicrobial sepsis-induced acute kidney injury through inhibiting endoplasmic reticulum stress-activated NF-?B pathway

Parecoxib reduced ventilation induced lung injury in acute respiratory distress syndrome

Hyperbaric Oxygen Pretreatment Improves Cognition and Reduces Hippocampal Damage Via p38 Mitogen-Activated Protein Kinase in a Rat Model

Suppressing Syndecan-1 Shedding Ameliorates Intestinal Epithelial Inflammation through Inhibiting NF-?B Pathway and TNF-?

Sequential Treatments with Tongsai and Bufei Yishen Granules Reduce Inflammation and Improve Pulmonary Function in Acute Exacerbation-Risk Window of Chronic Obstructive Pulmonary Disease in Rats

Exposure to nickel oxide nanoparticles induces pulmonary inflammation through NLRP3 inflammasome activation in rats

The neutrophil elastase inhibitor, sivelestat, attenuates sepsis-related kidney injury in rats

Inhibitory Effects of Angelica Polysaccharide on Activation of Mast Cells

Antioxidant and Anti-Inflammatory Effects of Coenzyme Q10 on L-Arginine-Induced Acute Pancreatitis in Rat

Wound healing treatment by high frequency ultrasound, microcurrent, and combined therapy modifies the immune response in rats

Decrease of CD68 Synovial Macrophages in Celastrol Treated Arthritic Rats

Preconditioning with VEGF Enhances Angiogenic and Neuroprotective Effects of Bone Marrow Mononuclear Cell Transplantation in a Rat Model of Chronic Cerebral Hypoperfusion

The effect of curcumin and its nanoformulation on adjuvant-induced arthritis in rats

Methionine Sulfoxide Reductase A Negatively Controls Microglia-Mediated Neuroinflammation via Inhibiting ROS/MAPKs/NF-?B Signaling Pathways Through a Catalytic Antioxidant Function

Epinephrine Enhances the Response of Macrophages under LPS Stimulation

Treatment of acute lung injury by targeting MG53-mediated cell membrane repair

Effects of Extract from Mangifera indica Leaf on Monosodium Urate Crystal-Induced Gouty Arthritis in Rats

Yang S, Zhang J, Wang S, Zhao X, Shi J. Oncotarget. 2017 Aug 24;8(53):91185-91198. doi: 10.18632/oncotarget.20434. eCollection 2017 Oct 31. SOCS2 overexpression alleviates diabetic nephropathy in rats by inhibiting the TLR4/NF-κB pathway

Gao D, Jing S, Zhang Q, Wu G. Exp Ther Med. 2018 Jan;15(1):1029-1035. doi: 10.3892/etm.2017.5479. Epub 2017 Nov 10. Pterostilbene protects against acute renal ischemia reperfusion injury and inhibits oxidative stress, inducible nitric oxide syntha...

Sun HJ, Ren XS, Xiong XQ, Chen YZ, Zhao MX, Wang JJ, Zhou YB, Han Y, Chen Q, Li YH, Kang YM, Zhu GQ. Cell Death Dis. 2017 Oct 5;8(10):e3074. doi: 10.1038/cddis.2017.470. NLRP3 inflammasome activation contributes to VSMC phenotypic transformation a...

Chen C, Chen W, Li Y, Dong Y, Teng X, Nong Z, Pan X, Lv L, Gao Y, Wu G. Oncotarget. 2017 Dec 4;8(67):111522-111534. doi: 10.18632/oncotarget.22869. eCollection 2017 Dec 19. Hyperbaric oxygen protects against myocardial reperfusion injury via the i...

Gu X, Zheng C, Zheng Q, Chen S, Li W, Shang Z, Zhang H. Am J Transl Res. 2017 Dec 15;9(12):5643-5652. eCollection 2017. Salvianolic acid A attenuates early brain injury after subarachnoid hemorrhage in rats by regulating ERK/P38/Nrf2 signaling.

Lin X, Ju YN, Gao W, Li DM, Guo CC. Biomed Res Int. 2018 Jan 29;2018:7507314. doi: 10.1155/2018/7507314. eCollection 2018. Desflurane Attenuates Ventilator-Induced Lung Injury in Rats with Acute Respiratory Distress Syndrome

Zhu R, Guo W, Fang H, Cao S, Yan B, Chen S, Zhang K, Zhang S. Mol Med Rep. 2018 May;17(5):6357-6362. doi: 10.3892/mmr.2018.8646. Epub 2018 Feb 27. Kupffer cell depletion by gadolinium chloride aggravates liver injury after brain death in rats

Chen X, Chen C, Fan S, Wu S, Yang F, Fang Z, Fu H, Li Y. J Neuroinflammation. 2018 Apr 20;15(1):116. doi: 10.1186/s12974-018-1151-3. Omega-3 polyunsaturated fatty acid attenuates the inflammatory response by modulating microglia polarization throu...

Zabihi M, Hajhashemi V, Talebi A, Minaiyan M. EXCLI J. 2017 Mar 30;16:414-425. doi: 10.17179/excli2016-727. eCollection 2017. Evaluation of central and peripheral effects of doxepin on acetic acid-induced colitis in rat and the involved mechanisms

Piao X, Liu B, Guo L, Meng F, Gao L. Oxid Med Cell Longev. 2017;2017:7085709. doi: 10.1155/2017/7085709. Epub 2017 Jun 21. Picroside II Shows Protective Functions for Severe Acute Pancreatitis in Rats by Preventing NF-κB-Dependent Autophagy

Li Y, Su R, Xu S, Huang Q, Xu H. Neuropsychiatr Dis Treat. 2017 Sep 27;13:2477-2487. doi: 10.2147/NDT.S145069. eCollection 2017. Artesunate prevents rats from the clozapine-induced hepatic steatosis and elevation in plasma triglycerides

Zabihi M, Hajhashemi V, Minaiyan M, Talebi A. Res Pharm Sci. 2017 Aug;12(4):337-345. doi: 10.4103/1735-5362.212052. Evaluation of the central and peripheral effects of doxepin on carrageenan-induced inflammatory paw edema in rat

Ling L, Feng X, Wei T, Wang Y, Wang Y, Zhang W, He L, Wang Z, Zeng Q, Xiong Z. Stem Cell Res Ther. 2017 Dec 19;8(1):283. doi: 10.1186/s13287-017-0739-3. Effects of low-intensity pulsed ultrasound (LIPUS)-pretreated human amnion-derived mesenchymal...

Chen Q, Wu H, Tao J, Liu C, Deng Z, Liu Y, Chen G, Liu B, Xu C. PLoS One. 2017 Aug 23;12(8):e0183688. doi: 10.1371/journal.pone.0183688. eCollection 2017. Effect of naringin on gp120-induced injury mediated by P2X7 receptors in rat primary culture...

Liu X, Zhang J, Han W, Wang Y, Liu Y, Zhang Y, Zhou D, Xiang L. Mol Med Rep. 2017 Jul;16(1):192-200. doi: 10.3892/mmr.2017.6553. Epub 2017 May 9. Inhibition of BTK protects lungs from trauma-hemorrhagic shock-induced injury in rats

Chen X, Wu S, Chen C, Xie B, Fang Z, Hu W, Chen J, Fu H, He H. J Neuroinflammation. 2017 Jul 24;14(1):143. doi: 10.1186/s12974-017-0917-3. Omega-3 polyunsaturated fatty acid supplementation attenuates microglial-induced inflammation by inhibiting ...

You C, Zu J, Liu X, Kong P, Song C, Wei R, Zhou C, Wang Y, Yan J. J Cell Mol Med. 2018 Apr;22(4):2449-2457. doi: 10.1111/jcmm.13549. Epub 2018 Jan 30. Synovial fibroblast‐targeting liposomes encapsulating an NF‐κB‐blocking peptide ameliorates zymo...

Xu H, Qin W, Hu X, Mu S, Zhu J, Lu W, Luo Y. J Neuroinflammation. 2018 Mar 15;15(1):83. doi: 10.1186/s12974-018-1117-5. Lentivirus-mediated overexpression of OTULIN ameliorates microglia activation and neuroinflammation by depressing the activatio...

Gao, W., Meng, Q.M., Cui, X.G. (2017). Budesonide instillation immediately after reperfusion ameliorates ischemia/reperfusion-induced injury in the transplanted lung of rat. Experimental Lung Research, 43(9-10), 439-446. doi: 10.1080/01902148.2017...

Vidal, B., Cascão, R., Vale, A.C., Cavaleiro, I., Vaz, M.F., Brito, J.A.,..., & Fonseca, J.E. (2015). Arthritis Induces Early Bone High Turnover, Structural Degradation and Mechanical Weakness. PLOS One, 10(1), e0117100. doi: 10.1371/journal.pone....

Zhao, B., He, E., Pan, Y., Xu, H., & Song, X. (2017). Kindlin-1 is a key protein in hyperbaric oxygen therapy for the treatment of neuropathic pain. Molecular Pain, 13, 1-10. doi: 10.1177/1744806917730254

Sun, G., Yang, W., Zhang, Y., & Zhao, M. (2017). Esculentoside A ameliorates cecal ligation and puncture-induced acute kidney injury in rats. Experimental Animals. Advance online publication. doi: 10.1538/expanim.16-0102

Ma Y, Chen F, Yang S, Duan Y, Sun Z, Shi J. Med Sci Monit. 2017 Jun 10;23:2816-2824. Silencing of TRB3 Ameliorates Diabetic Tubule Interstitial Nephropathy via PI3K/AKT Signaling in Rats

Mei X, Wang HX, Li JS, Liu XH, Lu XF, Li Y, Zhang WY, Tian YG. BMC Complement Altern Med. 2017 Apr 17;17(1):216. doi: 10.1186/s12906-017-1736-x. Dusuqing granules (DSQ) suppress inflammation in Klebsiella pneumonia rat via NF-κB/MAPK signaling.

Ghasemi-Pirbaluti M, Motaghi E, Najafi A, Hosseini MJ. Biomed Pharmacother. 2017 Mar 25;90:153-159. doi: 10.1016/j.biopha.2017.03.038. The effect of theophylline on acetic acid induced ulcerative colitis in rats.

Ye J, Caihong S, Yayan H, Zhang X, Meitian X. J Sci Food Agric. 2017 Mar 23. doi: 10.1002/jsfa.8322. Anti-fatigue activity of sea cucumber peptides prepared from Stichopus japonicas in an endurance swimming rat model.

Zahedi M, Ghiasvand R, Feizi A, Asgari G, Darvish L. Int J Prev Med. 2013 Jul;4(7):777-85. Does Quercetin Improve Cardiovascular Risk Factors And Inflammatory Biomarkers In Women With Type 2 Diabetes: A Double-Blind Randomized Controlled Clinical ...

Luo H,, Wang X,, Wang J,, Chen C,, Wang N,, Xu Z,, Chen S,, Zeng C,. Cardiovasc Diabetol. 2015 Jun 10;14:76. Doi: 10.1186/S12933-015-0239-7. Chronic Nf-??b Blockade Improves Renal Angiotensin Ii Type 1 Receptor Functions And Reduces Blood Pressure...

Li J, Yang L, Yao Q, Li Y, Tian Y, Li S, Jiang S, Wang Y, Li X, Guo Z. Evid Based Complement Alternat Med. 2014;2014:381976. Doi: 10.1155/2014/381976. Epub 2014 May 14. Effects And Mechanism Of Bufei Yishen Formula In A Rat Chronic Obstructive Pul...

Liu X, Mei Z, Qian J, Zeng Y, Wang M. Neural Regen Res. 2013 Dec 5;8(34):3203-15. Doi: 10.3969/J.Issn.1673-5374.2013.34.004. Puerarin Partly Counteracts The Inflammatory Response After Cerebral Ischemia/Reperfusion Via Activating The Cholinergic A...

Zhu J, Mu X, Zeng J, Xu C, Liu J, Zhang M, Li C, Chen J, Li T, Wang Y. Plos One. 2014 Jun 30;9(6):E101291. Doi: 10.1371/Journal.Pone.0101291. Ecollection 2014. Ginsenoside Rg1 Prevents Cognitive Impairment And Hippocampus Senescence In A Rat Model...

Huang L, Cao J. Oxid Med Cell Longev. 2014;2014:248786. Doi: 10.1155/2014/248786. Epub 2014 Mar 5. The Protective Effects Of Shen-Fu Injection On Experimental Acute Pancreatitis In A Rat Model.

Shi Q, Xie Wl, Zhang B, Chen Ln, Xu Y, Wang K, Ren K, Zhang Xm, Chen C, Zhang J, Dong Xp. Virol J. 2013 Jul 1;10:216. Doi: 10.1186/1743-422X-10-216. Brain Microglia Were Activated In Sporadic Cjd But Almost Unchanged In Fatal Familial Insomnia And...

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12 Customer Q&As for Rat IL-1 Beta/IL-1F2/IL1B ELISA Kit PicoKine™

Question

Q: Can IL-1 beta ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

Verified Customer

Verified customer

Asked: 2020-04-27

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is a must. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2020-04-27

Question

I am interested to purchase the Rat IL-1 Beta ELISA Kit PicoKine antibody, is it suitable to use in rat plasma samples?

Verified Customer

Verified customer

Asked: 2020-04-23

Answer

The product Rat IL-1 Beta ELISA Kit PicoKine antibody would be suitable to with plasma samples.

Boster Scientific Support

Answered: 2020-04-23

Question

Q: how to analyze ELISA data? I have performed IL-1 beta level in plasma.

Verified Customer

Verified customer

Asked: 2020-03-18

Answer

A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2020-03-18

Question

Is there a standardized total protein amount of samples that should be pipetted per well, so that it lies within the assay range of EK0393? Is protein quantification needed?

Verified customer

Asked: 2019-11-05

Answer

For the Rat IL-1 Beta ELISA Kit PicoKine™ (EK0393), add 100ul of diluted sample into each well. It is unnecessary to quantify the amount of total protein.

Boster Scientific Support

Answered: 2019-11-06

Question

What is the recommended dilution for rat serum samples using EK0393?

Verified customer

Asked: 2019-11-05

Answer

The recommended dilution for rat serum samples using the Rat IL-1 Beta ELISA Kit PicoKine™ (EK0393) is 1:1.

Boster Scientific Support

Answered: 2019-11-05

Question

Is it possible to use plasma with heparin in the analyses of the EK0393?

Verified customer

Asked: 2019-10-24

Answer

The Rat IL-1 Beta ELISA Kit PicoKine EK0393 has been validated with plasma with heparin.

Boster Scientific Support

Answered: 2019-10-24

Question

Q: if the enzyme conjugated IL-1 beta antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the IL-1 beta antigen?

D. Tiwari

Verified customer

Asked: 2018-11-29

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2018-11-29

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2018-06-03

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2018-06-03

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2017-08-19

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2017-08-19

Question

Q: how to thaw whole blood sample for IL-1 beta ELISA after freezing?

B. Hernandez

Verified customer

Asked: 2017-07-31

Answer

A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test IL-1 beta for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2017-07-31

Question

Q: is it okay to use citrate plasma as samples in Rat IL-1 beta Picokine® ELISA Kit (Catalog # EK0393)?

Verified Customer

Verified customer

Asked: 2016-08-15

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of IL-1 beta causing disruption of its protein structure. IL-1 beta may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the IL-1 beta ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2016-08-15

Question

Q: how are cell lysates prepared for use in Picokine® ELISA kits?

D. Martin

Verified customer

Asked: 2015-10-29

Answer

A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer may impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2015-10-29

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