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Product Info Summary
| SKU: | EK0393 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Rat |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Rat IL-1 Beta/IL-1F2/IL1B ELISA Kit PicoKine®
SKU/Catalog Number
EK0393
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Rat IL-1 Beta/IL-1F2/IL1B ELISA Kit PicoKine® (96 Tests). Quantitate Rat Il1b in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Rat IL-1 Beta/IL-1F2/IL1B ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0393)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: E.coli; Immunogen sequence: V117-S268
Sensitivity
<1 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0393 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0393 is reactive to Il1b in Rat samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK0393 is guaranteed for ELISA in Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Il1b
The Interleukin 1 family is a group of 11 beta trefoil cytokines. Interleukin-1, produced mainly by blood monocytes, mediates the panoply of host reactions collectively known as acute phase response . Interleukin-1beta (IL-1beta) is a potent stimulator of bone resorption, and has been implicated in the pathogenesis of high bone turnover and osteoporosis. IL-1beta belongs to proinflammatory cytokines, promote cancer cell adhesion and liver metastases by up-regulating the expression of vascular cell adhesion molecule-1 (VCAM-1) on hepatic sinusoidal endothelium (HSE).
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0393-CAP | Anti-Rat Il1b Pre-coated 96-well strip microplate | 1 |
| EK0393-ST | Rat Il1b Standard | 2 vials, 10 ng/tube |
| EK0393-DA | Rat Il1b Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.013 | 0.082 | 0.142 | 0.259 | 0.482 | 0.899 | 1.579 | 2.772 |
Data Example Images
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Rat IL-1 beta PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Il1b, Dilution ratio of 1:1, concentration in serum and plasma is less than the lowest standard, 31.2 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Rat IL-1 Beta/IL-1F2/IL1B ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 58 | 233 | 962 | 62 | 241 | 881 |
| Standard deviation | 3.65 | 11.41 | 69.26 | 4.21 | 12.29 | 68.71 |
| CV (%) | 7.8% | 4.9% | 7.2% | 6.8% | 7.8% | 7.8% |
Specific Publications For Rat IL-1 Beta/IL-1F2/IL1B ELISA Kit PicoKine® (EK0393)
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Customer Reviews
0 Reviews For Rat IL-1 Beta/IL-1F2/IL1B ELISA Kit PicoKine®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
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Submit your question
12 Customer Q&As for Rat IL-1 Beta/IL-1F2/IL1B ELISA Kit PicoKine®
Question
Q: Can IL-1 beta ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2020-04-27
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is a must. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2020-04-27
Question
I am interested to purchase the Rat IL-1 Beta ELISA Kit PicoKine antibody, is it suitable to use in rat plasma samples?
Verified Customer
Verified customer
Asked: 2020-04-23
Answer
The product Rat IL-1 Beta ELISA Kit PicoKine antibody would be suitable to with plasma samples.
Boster Scientific Support
Answered: 2020-04-23
Question
Q: how to analyze ELISA data? I have performed IL-1 beta level in plasma.
Verified Customer
Verified customer
Asked: 2020-03-18
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/elisa-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/elisa-data-analysis-online
Boster Scientific Support
Answered: 2020-03-18
Question
What is the recommended dilution for rat serum samples using EK0393?
Verified customer
Asked: 2019-11-05
Answer
The recommended dilution for rat serum samples using the Rat IL-1 Beta ELISA Kit PicoKine™ (EK0393) is 1:1.
Boster Scientific Support
Answered: 2019-11-05
Question
Is there a standardized total protein amount of samples that should be pipetted per well, so that it lies within the assay range of EK0418, EK0514 and EK0393? Is protein quantification needed?
Verified customer
Asked: 2019-11-05
Answer
For the Rat IL-10 ELISA Kit PicoKine™ (EK0418), Rat TGF Beta 1 ELISA Kit PicoKine™ (EK0514) and Rat IL-1 Beta ELISA Kit PicoKine™ (EK0393), add 100ul of diluted sample into each well. It is unnecessary to quantify the amount of total protein.
Boster Scientific Support
Answered: 2019-11-05
Question
Is it possible to use plasma with heparin in the analyses of the EK0393?
Verified customer
Asked: 2019-10-24
Answer
The Rat IL-1 Beta ELISA Kit PicoKine EK0393 has been validated with plasma with heparin.
Boster Scientific Support
Answered: 2019-10-24
Question
Q: if the enzyme conjugated IL-1 beta antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the IL-1 beta antigen?
D. Tiwari
Verified customer
Asked: 2018-11-29
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2018-11-29
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2018-06-03
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2018-06-03
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2017-08-19
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2017-08-19
Question
Q: how to thaw whole blood sample for IL-1 beta ELISA after freezing?
B. Hernandez
Verified customer
Asked: 2017-07-31
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test IL-1 beta for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2017-07-31
Question
Q: is it okay to use citrate plasma as samples in Rat IL-1 beta Picokine® ELISA Kit (Catalog # EK0393)?
Verified Customer
Verified customer
Asked: 2016-08-15
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of IL-1 beta causing disruption of its protein structure. IL-1 beta may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the IL-1 beta ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2016-08-15
Question
Q: how are cell lysates prepared for use in Picokine® ELISA kits?
D. Martin
Verified customer
Asked: 2015-10-29
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer may impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2015-10-29
